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The map label for this gene is mdeA [H]

Identifier: 83590156

GI number: 83590156

Start: 1352794

End: 1354080

Strand: Reverse

Name: mdeA [H]

Synonym: Moth_1309

Alternate gene names: 83590156

Gene position: 1354080-1352794 (Counterclockwise)

Preceding gene: 83590157

Following gene: 83590155

Centisome position: 51.51

GC content: 57.73

Gene sequence:

>1287_bases
ATGACTACCAATCACCAGTATCGTTTTGCCACCCTGGCCGTTCAAGCCGGCCAGGAGCCCGATCCGGCTACGGGAGCTCG
CGCCGTGCCTATCTACCAGACTACTTCCTATGTCTTCCGGGATGCCGATCATGCCGCCGCCCTCTTCGGCCTGAGGGAAG
AAGGTAATATATATACCCGGATTATGAATCCCACTACCGATGTCTTTGAAAAAAGGATGGCTGCCCTGGAGGGCGGTGTC
GGCGCCCTGGCAACGGCTTCCGGCCAGGCGGCCATCACCCTGGCCATAGCTAATATCGCCACCGCCGGACAGGAGATTGT
TGCTTCCACCAGCCTTTACGGCGGCACCTTCAGTCTTTTTAATTCAACCCTGCCCAAGTTTGGTATTAAAACCCGTTTTG
TCGACAGCGCCGAACCGGAGGATTTCCGGGCTGCCATTACAGACCGGACCAGGGCCCTTTATGTGGAAATCCTGGGCAAT
CCCAAGCTGGACGTGCCGGACCTGGAGGCCCTGGCTGCCATCGCCCACGAGGCTGGTATTCCTTTAATCGTTGATAACAC
CTTCGCCACGCCTTACCTGTGCCGTCCGTTTGAATTCGGAGCCGATATCGTCGTGCACTCGGCGACCAAGTTTATCGGCG
GCCATGGTACTTCCATTGGCGGCATAATTGTCGATTCCGGTAAATTCAATTGGGATAACGGTAAATTTCCCGGACTGGTA
GAACCCGATCCCAGTTATCACGGCCTCAGTTACGTCCAGTCCTTTGGCCCGGCGGCCTACATCGTCAAGGCGCGGGTCCA
GCTCTTGCGGGACCTGGGACCGGCCTTAAGTCCTTTCAATGCCTTCCTTTTCCTGCAGGGACTGGAAACTCTGCACCTGC
GGATGGAGCGCCACGTCCAAAATGCTACCAGGATCGCCGGCTGGCTGGCAGAGCACCCGGCTGTCGCCTGGGTGAGCTAT
CCGGGCCTACCCGGCCATCCCTACTACGAACGGGCCCGAAAATACCTGCCTAAAGGAGCGGGGGCCATTTTGACCTTTGG
TATTAAGGGCGGCAAGGAGGCCGGTAAGAAGTTTATCAACAGCGTGAAACTCTTCTCCCTCCTGGCCAACGTGGGTGATG
CCCATTCCCTGGTCATTCACCCGGCCAGTACCACCCATCAGCAGCTGACACCGGAGGAACAGCTGGCCTCGGGTGTTACC
GAAGATCTGGTCCGCATCTCCGTGGGCCTGGAGGACGTAGAAGACCTGATTGCCGACCTGGACCAGGCATTAAACAGGAG
CCGGTAG

Upstream 100 bases:

>100_bases
CTGAGAGATAAGGGGGGTAAGAATTGAGTGCCTCTTCTTATCGGAGAGGTTTTTTTATTCCCCTGCAGCCAGCAAGTTAA
TCATGGAGAGGGGAAGTAAA

Downstream 100 bases:

>100_bases
CATGGATGGAGTCGGTATTGTAACAACCAGGTTTTATGAATGGTCGCAATGCCTCCACCTGGAAAGCGGGGCCCAGCTGG
GCTCCCTTACCATAGCCTAT

Product: O-acetylhomoserine/O-acetylserine sulfhydrylase

Products: NA

Alternate protein names: L-methioninase [H]

Number of amino acids: Translated: 428; Mature: 427

Protein sequence:

>428_residues
MTTNHQYRFATLAVQAGQEPDPATGARAVPIYQTTSYVFRDADHAAALFGLREEGNIYTRIMNPTTDVFEKRMAALEGGV
GALATASGQAAITLAIANIATAGQEIVASTSLYGGTFSLFNSTLPKFGIKTRFVDSAEPEDFRAAITDRTRALYVEILGN
PKLDVPDLEALAAIAHEAGIPLIVDNTFATPYLCRPFEFGADIVVHSATKFIGGHGTSIGGIIVDSGKFNWDNGKFPGLV
EPDPSYHGLSYVQSFGPAAYIVKARVQLLRDLGPALSPFNAFLFLQGLETLHLRMERHVQNATRIAGWLAEHPAVAWVSY
PGLPGHPYYERARKYLPKGAGAILTFGIKGGKEAGKKFINSVKLFSLLANVGDAHSLVIHPASTTHQQLTPEEQLASGVT
EDLVRISVGLEDVEDLIADLDQALNRSR

Sequences:

>Translated_428_residues
MTTNHQYRFATLAVQAGQEPDPATGARAVPIYQTTSYVFRDADHAAALFGLREEGNIYTRIMNPTTDVFEKRMAALEGGV
GALATASGQAAITLAIANIATAGQEIVASTSLYGGTFSLFNSTLPKFGIKTRFVDSAEPEDFRAAITDRTRALYVEILGN
PKLDVPDLEALAAIAHEAGIPLIVDNTFATPYLCRPFEFGADIVVHSATKFIGGHGTSIGGIIVDSGKFNWDNGKFPGLV
EPDPSYHGLSYVQSFGPAAYIVKARVQLLRDLGPALSPFNAFLFLQGLETLHLRMERHVQNATRIAGWLAEHPAVAWVSY
PGLPGHPYYERARKYLPKGAGAILTFGIKGGKEAGKKFINSVKLFSLLANVGDAHSLVIHPASTTHQQLTPEEQLASGVT
EDLVRISVGLEDVEDLIADLDQALNRSR
>Mature_427_residues
TTNHQYRFATLAVQAGQEPDPATGARAVPIYQTTSYVFRDADHAAALFGLREEGNIYTRIMNPTTDVFEKRMAALEGGVG
ALATASGQAAITLAIANIATAGQEIVASTSLYGGTFSLFNSTLPKFGIKTRFVDSAEPEDFRAAITDRTRALYVEILGNP
KLDVPDLEALAAIAHEAGIPLIVDNTFATPYLCRPFEFGADIVVHSATKFIGGHGTSIGGIIVDSGKFNWDNGKFPGLVE
PDPSYHGLSYVQSFGPAAYIVKARVQLLRDLGPALSPFNAFLFLQGLETLHLRMERHVQNATRIAGWLAEHPAVAWVSYP
GLPGHPYYERARKYLPKGAGAILTFGIKGGKEAGKKFINSVKLFSLLANVGDAHSLVIHPASTTHQQLTPEEQLASGVTE
DLVRISVGLEDVEDLIADLDQALNRSR

Specific function: Methionine biosynthesis; second step. [C]

COG id: COG2873

COG function: function code E; O-acetylhomoserine sulfhydrylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the trans-sulfuration enzymes family [H]

Homologues:

Organism=Homo sapiens, GI21361334, Length=422, Percent_Identity=36.0189573459716, Blast_Score=225, Evalue=5e-59,
Organism=Homo sapiens, GI299473759, Length=422, Percent_Identity=32.7014218009479, Blast_Score=186, Evalue=5e-47,
Organism=Homo sapiens, GI25453487, Length=421, Percent_Identity=31.5914489311164, Blast_Score=172, Evalue=8e-43,
Organism=Escherichia coli, GI1790375, Length=413, Percent_Identity=33.1719128329298, Blast_Score=226, Evalue=2e-60,
Organism=Escherichia coli, GI1789383, Length=425, Percent_Identity=25.8823529411765, Blast_Score=140, Evalue=2e-34,
Organism=Caenorhabditis elegans, GI71980708, Length=425, Percent_Identity=33.1764705882353, Blast_Score=224, Evalue=7e-59,
Organism=Caenorhabditis elegans, GI17538055, Length=421, Percent_Identity=34.6793349168646, Blast_Score=211, Evalue=6e-55,
Organism=Caenorhabditis elegans, GI17560056, Length=421, Percent_Identity=35.1543942992874, Blast_Score=206, Evalue=3e-53,
Organism=Saccharomyces cerevisiae, GI6323333, Length=432, Percent_Identity=53.4722222222222, Blast_Score=419, Evalue=1e-118,
Organism=Saccharomyces cerevisiae, GI6319307, Length=433, Percent_Identity=35.796766743649, Blast_Score=224, Evalue=2e-59,
Organism=Saccharomyces cerevisiae, GI6321170, Length=356, Percent_Identity=27.247191011236, Blast_Score=129, Evalue=6e-31,
Organism=Saccharomyces cerevisiae, GI6321254, Length=443, Percent_Identity=25.5079006772009, Blast_Score=126, Evalue=6e-30,
Organism=Saccharomyces cerevisiae, GI6321904, Length=422, Percent_Identity=25.5924170616114, Blast_Score=103, Evalue=7e-23,
Organism=Drosophila melanogaster, GI19922554, Length=419, Percent_Identity=33.6515513126492, Blast_Score=221, Evalue=1e-57,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000277
- InterPro:   IPR006237
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF01053 Cys_Met_Meta_PP [H]

EC number: =4.4.1.11 [H]

Molecular weight: Translated: 46104; Mature: 45973

Theoretical pI: Translated: 6.32; Mature: 6.32

Prosite motif: PS00868 CYS_MET_METAB_PP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
1.2 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
0.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTNHQYRFATLAVQAGQEPDPATGARAVPIYQTTSYVFRDADHAAALFGLREEGNIYTR
CCCCCCEEEEEEEEECCCCCCCCCCCEEEEEEECHHHHHCCCCHHHHHEEECCCCCEEEE
IMNPTTDVFEKRMAALEGGVGALATASGQAAITLAIANIATAGQEIVASTSLYGGTFSLF
EECCCHHHHHHHHHHHCCCCCEEEECCCCEEEEEEEHHHHHCCHHHHHHHCCCCCHHHHH
NSTLPKFGIKTRFVDSAEPEDFRAAITDRTRALYVEILGNPKLDVPDLEALAAIAHEAGI
HHCCCCCCCCEEECCCCCCHHHHHHHHCCCEEEEEEEECCCCCCCCCHHHHHHHHHHCCC
PLIVDNTFATPYLCRPFEFGADIVVHSATKFIGGHGTSIGGIIVDSGKFNWDNGKFPGLV
CEEEECCCCCCCEECCHHCCCCEEEEHHHHHHCCCCCCCCEEEEECCEECCCCCCCCCCC
EPDPSYHGLSYVQSFGPAAYIVKARVQLLRDLGPALSPFNAFLFLQGLETLHLRMERHVQ
CCCCCCCHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
NATRIAGWLAEHPAVAWVSYPGLPGHPYYERARKYLPKGAGAILTFGIKGGKEAGKKFIN
HHHHHHHHHHCCCCEEEEECCCCCCCHHHHHHHHHCCCCCCEEEEEECCCCHHHHHHHHH
SVKLFSLLANVGDAHSLVIHPASTTHQQLTPEEQLASGVTEDLVRISVGLEDVEDLIADL
HHHHHHHHHCCCCCCEEEEECCCCCHHHCCCHHHHHCCHHHHHHHHHCCHHHHHHHHHHH
DQALNRSR
HHHHHCCC
>Mature Secondary Structure 
TTNHQYRFATLAVQAGQEPDPATGARAVPIYQTTSYVFRDADHAAALFGLREEGNIYTR
CCCCCEEEEEEEEECCCCCCCCCCCEEEEEEECHHHHHCCCCHHHHHEEECCCCCEEEE
IMNPTTDVFEKRMAALEGGVGALATASGQAAITLAIANIATAGQEIVASTSLYGGTFSLF
EECCCHHHHHHHHHHHCCCCCEEEECCCCEEEEEEEHHHHHCCHHHHHHHCCCCCHHHHH
NSTLPKFGIKTRFVDSAEPEDFRAAITDRTRALYVEILGNPKLDVPDLEALAAIAHEAGI
HHCCCCCCCCEEECCCCCCHHHHHHHHCCCEEEEEEEECCCCCCCCCHHHHHHHHHHCCC
PLIVDNTFATPYLCRPFEFGADIVVHSATKFIGGHGTSIGGIIVDSGKFNWDNGKFPGLV
CEEEECCCCCCCEECCHHCCCCEEEEHHHHHHCCCCCCCCEEEEECCEECCCCCCCCCCC
EPDPSYHGLSYVQSFGPAAYIVKARVQLLRDLGPALSPFNAFLFLQGLETLHLRMERHVQ
CCCCCCCHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
NATRIAGWLAEHPAVAWVSYPGLPGHPYYERARKYLPKGAGAILTFGIKGGKEAGKKFIN
HHHHHHHHHHCCCCEEEEECCCCCCCHHHHHHHHHCCCCCCEEEEEECCCCHHHHHHHHH
SVKLFSLLANVGDAHSLVIHPASTTHQQLTPEEQLASGVTEDLVRISVGLEDVEDLIADL
HHHHHHHHHCCCCCCEEEEECCCCCHHHCCCHHHHHCCHHHHHHHHHCCHHHHHHHHHHH
DQALNRSR
HHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8586629; 9190812; 3365412 [H]