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The map label for this gene is hisA

Identifier: 83594924

GI number: 83594924

Start: 4137192

End: 4137917

Strand: Reverse

Name: hisA

Synonym: Rru_A3595

Alternate gene names: 83594924

Gene position: 4137917-4137192 (Counterclockwise)

Preceding gene: 83594925

Following gene: 83594923

Centisome position: 95.06

GC content: 69.15

Gene sequence:

>726_bases
ATGATCTTGTTTCCCGCCATCGACTTGAAGGACGGCCAGTGCGTTCGCCTGCGCAAGGGCGAGATGGATCAGGCGACCGT
CTTCAATGACAGCCCGGCCGATCAGGCCGCCGCCTTCGCCCTGGCCGGCTGCCGGTGGATCCATGTCGTCGATCTCAACG
GCGCCTTCGCCGGCCGGCCGGTCAACGCCCCGGCGGTCGAAGCCATTCTGGCGGCCGTCGATCTGCCGGTGCAGCTTGGC
GGCGGCATCCGTGACTTGGCGGCGATCGATCTGTGGCTCGACAAGGGGGTGCGCCGGGTGATCCTGGGCACCGTGGCCCT
GCGCGATCCGCAACTTGTGCGCGAGGCCTGCAAACGCCATCCTGGACGGATCGCCGTTGGCATCGACGCCCGCGGCGGCA
TGGTCGCCGTCGAGGGCTGGGCCGAAACCAGCGCCGTCAGCGCCCTTGATCTGGCTTTGCGCTTCGAAGACGCCGGGGTG
GCGGCCATCGTCCATACCGACATCGACCGCGACGGCCTGCTCGCCGGACCCAATCTCGACGCCACGGCCGCCCTGGCCGA
GCGGCTGACCACGCCGGTGATCGTCTCGGGCGGGGTGGCCAGCCTTGACGATCTGCGCGCCATCAGGGCGCGGGCGGCGG
CCGGTCTGGAAGGCGTGATCAGCGGTCGGGCGCTCTATGACGGTCGCATCGATCTTGCCGAGGCTCTGGCGGTTCTGGCC
GATTAA

Upstream 100 bases:

>100_bases
CTGCGCCTGATCGCCAATTTCCTGCGCTGGGCCCCGTGACCCCCGCCCTGCGCGCGGCCCTTCCGTCCTTTGCCCCCGCC
CCTTGTTTTAAGAGACCCCG

Downstream 100 bases:

>100_bases
GCGCGAAGGACGGATCATGTTGAAGATGCGCATCATCCCCTGTCTCGACGTCAAGGACGGCCGGGTGGTCAAAGGGGTTA
ACTTCGTCGGCCTGCGCGAT

Product: 1-(5-phosphoribosyl)-5-[(5- phosphoribosylamino)methylideneamino] imidazole-4-carboxamide isomerase

Products: NA

Alternate protein names: Phosphoribosylformimino-5-aminoimidazole carboxamide ribotide isomerase

Number of amino acids: Translated: 241; Mature: 241

Protein sequence:

>241_residues
MILFPAIDLKDGQCVRLRKGEMDQATVFNDSPADQAAAFALAGCRWIHVVDLNGAFAGRPVNAPAVEAILAAVDLPVQLG
GGIRDLAAIDLWLDKGVRRVILGTVALRDPQLVREACKRHPGRIAVGIDARGGMVAVEGWAETSAVSALDLALRFEDAGV
AAIVHTDIDRDGLLAGPNLDATAALAERLTTPVIVSGGVASLDDLRAIRARAAAGLEGVISGRALYDGRIDLAEALAVLA
D

Sequences:

>Translated_241_residues
MILFPAIDLKDGQCVRLRKGEMDQATVFNDSPADQAAAFALAGCRWIHVVDLNGAFAGRPVNAPAVEAILAAVDLPVQLG
GGIRDLAAIDLWLDKGVRRVILGTVALRDPQLVREACKRHPGRIAVGIDARGGMVAVEGWAETSAVSALDLALRFEDAGV
AAIVHTDIDRDGLLAGPNLDATAALAERLTTPVIVSGGVASLDDLRAIRARAAAGLEGVISGRALYDGRIDLAEALAVLA
D
>Mature_241_residues
MILFPAIDLKDGQCVRLRKGEMDQATVFNDSPADQAAAFALAGCRWIHVVDLNGAFAGRPVNAPAVEAILAAVDLPVQLG
GGIRDLAAIDLWLDKGVRRVILGTVALRDPQLVREACKRHPGRIAVGIDARGGMVAVEGWAETSAVSALDLALRFEDAGV
AAIVHTDIDRDGLLAGPNLDATAALAERLTTPVIVSGGVASLDDLRAIRARAAAGLEGVISGRALYDGRIDLAEALAVLA
D

Specific function: Histidine biosynthesis; fourth step. [C]

COG id: COG0106

COG function: function code E; Phosphoribosylformimino-5-aminoimidazole carboxamide ribonucleotide (ProFAR) isomerase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the hisA/hisF family

Homologues:

Organism=Escherichia coli, GI87082028, Length=243, Percent_Identity=34.156378600823, Blast_Score=129, Evalue=1e-31,
Organism=Escherichia coli, GI1788336, Length=252, Percent_Identity=25, Blast_Score=62, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): HIS4_RHORT (Q2RNA6)

Other databases:

- EMBL:   CP000230
- RefSeq:   YP_428676.1
- ProteinModelPortal:   Q2RNA6
- SMR:   Q2RNA6
- STRING:   Q2RNA6
- GeneID:   3837051
- GenomeReviews:   CP000230_GR
- KEGG:   rru:Rru_A3595
- NMPDR:   fig|1085.1.peg.1902
- eggNOG:   COG0106
- HOGENOM:   HBG541613
- OMA:   SIIYTDI
- BioCyc:   RRUB269796:RRU_A3595-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01014
- InterPro:   IPR013785
- InterPro:   IPR006062
- InterPro:   IPR006063
- InterPro:   IPR023016
- InterPro:   IPR011060
- Gene3D:   G3DSA:3.20.20.70
- TIGRFAMs:   TIGR00007

Pfam domain/function: PF00977 His_biosynth; SSF51366 RibP_bind_barrel

EC number: =5.3.1.16

Molecular weight: Translated: 25064; Mature: 25064

Theoretical pI: Translated: 4.70; Mature: 4.70

Prosite motif: NA

Important sites: ACT_SITE 8-8 ACT_SITE 129-129

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MILFPAIDLKDGQCVRLRKGEMDQATVFNDSPADQAAAFALAGCRWIHVVDLNGAFAGRP
CEEECEEECCCCCEEEEECCCCCCEEEECCCCHHHHHHHHHHCCEEEEEEECCCCCCCCC
VNAPAVEAILAAVDLPVQLGGGIRDLAAIDLWLDKGVRRVILGTVALRDPQLVREACKRH
CCCHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHCHHEEHHHHHCCCCHHHHHHHHHCC
PGRIAVGIDARGGMVAVEGWAETSAVSALDLALRFEDAGVAAIVHTDIDRDGLLAGPNLD
CCEEEEEEECCCCEEEEECCCCHHHHHHEEEEEEECCCCEEEEEEECCCCCCEEECCCCC
ATAALAERLTTPVIVSGGVASLDDLRAIRARAAAGLEGVISGRALYDGRIDLAEALAVLA
HHHHHHHHCCCCEEEECCCCCHHHHHHHHHHHHCCCHHHCCCCEEECCCCCHHHHHHHHC
D
C
>Mature Secondary Structure
MILFPAIDLKDGQCVRLRKGEMDQATVFNDSPADQAAAFALAGCRWIHVVDLNGAFAGRP
CEEECEEECCCCCEEEEECCCCCCEEEECCCCHHHHHHHHHHCCEEEEEEECCCCCCCCC
VNAPAVEAILAAVDLPVQLGGGIRDLAAIDLWLDKGVRRVILGTVALRDPQLVREACKRH
CCCHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHCHHEEHHHHHCCCCHHHHHHHHHCC
PGRIAVGIDARGGMVAVEGWAETSAVSALDLALRFEDAGVAAIVHTDIDRDGLLAGPNLD
CCEEEEEEECCCCEEEEECCCCHHHHHHEEEEEEECCCCEEEEEEECCCCCCEEECCCCC
ATAALAERLTTPVIVSGGVASLDDLRAIRARAAAGLEGVISGRALYDGRIDLAEALAVLA
HHHHHHHHCCCCEEEECCCCCHHHHHHHHHHHHCCCHHHCCCCEEECCCCCHHHHHHHHC
D
C

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA