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The map label for this gene is hisH

Identifier: 83594925

GI number: 83594925

Start: 4137979

End: 4138623

Strand: Reverse

Name: hisH

Synonym: Rru_A3596

Alternate gene names: 83594925

Gene position: 4138623-4137979 (Counterclockwise)

Preceding gene: 83594926

Following gene: 83594924

Centisome position: 95.08

GC content: 67.44

Gene sequence:

>645_bases
ATGCGTGTCGCGCTTATCGATTATGGCTCGGGCAATCTCCGTTCGGCGGCGAAAGCCCTCGAACGGGCCGCTCGCGACGG
TGGAGTCGCCGCCGAAATCGTCGTTACCCGCGATGTCGAGGGGGTCCTGGGGGCCGATCGCGTGGTGCTGCCCGGCGTCG
GCGCCTTCGCCAATTGCCGGCGCGGCTTGCAGGGCATCGATGGCATGGTCGAGGCCCTGGCCGAGGTGGCGCTGGTCCGC
GCCCGCCCCTTCCTTGGCATCTGCGTCGGCATGCAGCTGCTGGCCGACGAGGGCGTGGAATATGGCCGTCACCCCGGTCT
GGGATGGATCGGCGGCACCGTGGAAGCGATCAGCCCGGCCGACCCCGCCCTCAAGATCCCCCACATGGGATGGAACAGCC
TGGATTTTCAGGCCGATAGTCATGCCTTGCTCGCCGGCATCGAGCCGGGGACCCATGTCTATTTCGTCCATAGCTACCAC
TTCCGCCCCGCCGACCCGGCGACGCGCTTGGCCAGCGTTGAATACGGCGGGCCGCTGACCGCGATGATCGGCCGGGAAAA
TCTGGTCGGAACCCAGTTCCATCCCGAGAAAAGCCAGGCCGCCGGGCTGCGCCTGATCGCCAATTTCCTGCGCTGGGCCC
CGTGA

Upstream 100 bases:

>100_bases
TGCGATTTTCCACGGTCTTTTGGGTCTTTTCCGCTTTTTTGACAGGCTCCGGGCCCGGGTTGGCGCCCTGCCCGGCGCCC
TCATCGGGGTCCAGGCTGTG

Downstream 100 bases:

>100_bases
CCCCCGCCCTGCGCGCGGCCCTTCCGTCCTTTGCCCCCGCCCCTTGTTTTAAGAGACCCCGATGATCTTGTTTCCCGCCA
TCGACTTGAAGGACGGCCAG

Product: imidazole glycerol phosphate synthase subunit HisH

Products: NA

Alternate protein names: IGP synthase glutamine amidotransferase subunit; IGP synthase subunit hisH; ImGP synthase subunit hisH; IGPS subunit hisH

Number of amino acids: Translated: 214; Mature: 214

Protein sequence:

>214_residues
MRVALIDYGSGNLRSAAKALERAARDGGVAAEIVVTRDVEGVLGADRVVLPGVGAFANCRRGLQGIDGMVEALAEVALVR
ARPFLGICVGMQLLADEGVEYGRHPGLGWIGGTVEAISPADPALKIPHMGWNSLDFQADSHALLAGIEPGTHVYFVHSYH
FRPADPATRLASVEYGGPLTAMIGRENLVGTQFHPEKSQAAGLRLIANFLRWAP

Sequences:

>Translated_214_residues
MRVALIDYGSGNLRSAAKALERAARDGGVAAEIVVTRDVEGVLGADRVVLPGVGAFANCRRGLQGIDGMVEALAEVALVR
ARPFLGICVGMQLLADEGVEYGRHPGLGWIGGTVEAISPADPALKIPHMGWNSLDFQADSHALLAGIEPGTHVYFVHSYH
FRPADPATRLASVEYGGPLTAMIGRENLVGTQFHPEKSQAAGLRLIANFLRWAP
>Mature_214_residues
MRVALIDYGSGNLRSAAKALERAARDGGVAAEIVVTRDVEGVLGADRVVLPGVGAFANCRRGLQGIDGMVEALAEVALVR
ARPFLGICVGMQLLADEGVEYGRHPGLGWIGGTVEAISPADPALKIPHMGWNSLDFQADSHALLAGIEPGTHVYFVHSYH
FRPADPATRLASVEYGGPLTAMIGRENLVGTQFHPEKSQAAGLRLIANFLRWAP

Specific function: IGPS catalyzes the conversion of PRFAR and glutamine to IGP, AICAR and glutamate. The hisH subunit provides the glutamine amidotransferase activity that produces the ammonia necessary to hisF for the synthesis of IGP and AICAR

COG id: COG0118

COG function: function code E; Glutamine amidotransferase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-1 domain

Homologues:

Organism=Escherichia coli, GI1788334, Length=212, Percent_Identity=40.0943396226415, Blast_Score=126, Evalue=9e-31,
Organism=Saccharomyces cerevisiae, GI6319725, Length=220, Percent_Identity=31.8181818181818, Blast_Score=103, Evalue=3e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): HIS5_RHORT (Q2RNA5)

Other databases:

- EMBL:   CP000230
- RefSeq:   YP_428677.1
- ProteinModelPortal:   Q2RNA5
- SMR:   Q2RNA5
- STRING:   Q2RNA5
- GeneID:   3837052
- GenomeReviews:   CP000230_GR
- KEGG:   rru:Rru_A3596
- NMPDR:   fig|1085.1.peg.1901
- eggNOG:   COG0118
- HOGENOM:   HBG292341
- OMA:   INTFASS
- PhylomeDB:   Q2RNA5
- ProtClustDB:   PRK13146
- BioCyc:   RRUB269796:RRU_A3596-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00278
- InterPro:   IPR017926
- InterPro:   IPR000991
- InterPro:   IPR010139
- InterPro:   IPR016226
- PIRSF:   PIRSF000495
- TIGRFAMs:   TIGR01855

Pfam domain/function: PF00117 GATase

EC number: 2.4.2.-

Molecular weight: Translated: 22686; Mature: 22686

Theoretical pI: Translated: 6.63; Mature: 6.63

Prosite motif: PS51273 GATASE_TYPE_1; PS00442 GATASE_TYPE_I

Important sites: ACT_SITE 88-88 ACT_SITE 194-194 ACT_SITE 196-196

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRVALIDYGSGNLRSAAKALERAARDGGVAAEIVVTRDVEGVLGADRVVLPGVGAFANCR
CEEEEEECCCCCHHHHHHHHHHHHHCCCCEEEEEEEECCCCHHCCCEEEECCCCHHHHHH
RGLQGIDGMVEALAEVALVRARPFLGICVGMQLLADEGVEYGRHPGLGWIGGTVEAISPA
HHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCHHCCCCCCCCCCCCCEEECCCC
DPALKIPHMGWNSLDFQADSHALLAGIEPGTHVYFVHSYHFRPADPATRLASVEYGGPLT
CCCEECCCCCCCCCCCCCCCCEEEEECCCCCEEEEEEEECCCCCCHHHHEEEECCCCCEE
AMIGRENLVGTQFHPEKSQAAGLRLIANFLRWAP
EEECCCCCCCCCCCCCHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MRVALIDYGSGNLRSAAKALERAARDGGVAAEIVVTRDVEGVLGADRVVLPGVGAFANCR
CEEEEEECCCCCHHHHHHHHHHHHHCCCCEEEEEEEECCCCHHCCCEEEECCCCHHHHHH
RGLQGIDGMVEALAEVALVRARPFLGICVGMQLLADEGVEYGRHPGLGWIGGTVEAISPA
HHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCHHCCCCCCCCCCCCCEEECCCC
DPALKIPHMGWNSLDFQADSHALLAGIEPGTHVYFVHSYHFRPADPATRLASVEYGGPLT
CCCEECCCCCCCCCCCCCCCCEEEEECCCCCEEEEEEEECCCCCCHHHHEEEECCCCCEE
AMIGRENLVGTQFHPEKSQAAGLRLIANFLRWAP
EEECCCCCCCCCCCCCHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferases; Glycosyltransferases; Pentosyltransferases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA