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The map label for this gene is hisF

Identifier: 83594923

GI number: 83594923

Start: 4136417

End: 4137175

Strand: Reverse

Name: hisF

Synonym: Rru_A3594

Alternate gene names: 83594923

Gene position: 4137175-4136417 (Counterclockwise)

Preceding gene: 83594924

Following gene: 83594922

Centisome position: 95.05

GC content: 67.72

Gene sequence:

>759_bases
ATGTTGAAGATGCGCATCATCCCCTGTCTCGACGTCAAGGACGGCCGGGTGGTCAAAGGGGTTAACTTCGTCGGCCTGCG
CGATGCCGGCGATCCGGTGGAACAGGCCCGGCTTTACGACGCGGCCGGGGCCGACGAGTTGTGCTTCCTTGACATCACCG
CCAGCCACGAGAACCGCGACACCTTGCTTGATGTCGTCGGCCGCACCGCCGATCAGTGCTTCATGCCGCTGACCGTTGGC
GGCGGCGTGCGCACGACCGAGGATATCCGCAAGCTGCTGCTGGCCGGGGCCGACAAGGTGTCGATCAACACCGCCGCCGT
CACCCGCCCCGAGTTCGTGCGCGAGGCGGCCGAGACGTTCGGCGCCCAATGCGTGGTGGTGGCCCTGGACGCCAAATCGG
TGGGGCCGGGACGGTTCGAGATCTTCACCCATGGCGGCCGCAAGGCCACCGGCCTGGACGCCGTGGACTGGGCCAAGCGC
ATGGTGGCGCTGGGGGCGGGAGAAATCCTGCTGACCTCGATGGACCGCGACGGCACCCGCCAGGGCTTTAACTTGCCGCT
GACCCGGGCGATCGCCGATGCGGTGGCGGTGCCGGTCATCGCCTCGGGCGGGGTGGGTACCTTGGATCATCTGGTCGAAG
GCGTGCGCGACGGTCACGCCACCGCCGTGCTTGCCGCCTCGATCTTCCACTTCGGAGAGTATACCATCGCCCAGGCCAAG
GCCCATATGGCGGCGGCCGGACTCCCCATGCGGCTTTAG

Upstream 100 bases:

>100_bases
GGTCTGGAAGGCGTGATCAGCGGTCGGGCGCTCTATGACGGTCGCATCGATCTTGCCGAGGCTCTGGCGGTTCTGGCCGA
TTAAGCGCGAAGGACGGATC

Downstream 100 bases:

>100_bases
GGCGGCACGCGGAACAGCGGCTTCGCCCGTCGCCGCCATAACGCCGGCTCCCCATTCAAACGGAAGATCGCCGATGACCG
ATATCCCCCCCGCCGTCTCC

Product: imidazole glycerol phosphate synthase subunit HisF

Products: NA

Alternate protein names: IGP synthase cyclase subunit; IGP synthase subunit hisF; ImGP synthase subunit hisF; IGPS subunit hisF

Number of amino acids: Translated: 252; Mature: 252

Protein sequence:

>252_residues
MLKMRIIPCLDVKDGRVVKGVNFVGLRDAGDPVEQARLYDAAGADELCFLDITASHENRDTLLDVVGRTADQCFMPLTVG
GGVRTTEDIRKLLLAGADKVSINTAAVTRPEFVREAAETFGAQCVVVALDAKSVGPGRFEIFTHGGRKATGLDAVDWAKR
MVALGAGEILLTSMDRDGTRQGFNLPLTRAIADAVAVPVIASGGVGTLDHLVEGVRDGHATAVLAASIFHFGEYTIAQAK
AHMAAAGLPMRL

Sequences:

>Translated_252_residues
MLKMRIIPCLDVKDGRVVKGVNFVGLRDAGDPVEQARLYDAAGADELCFLDITASHENRDTLLDVVGRTADQCFMPLTVG
GGVRTTEDIRKLLLAGADKVSINTAAVTRPEFVREAAETFGAQCVVVALDAKSVGPGRFEIFTHGGRKATGLDAVDWAKR
MVALGAGEILLTSMDRDGTRQGFNLPLTRAIADAVAVPVIASGGVGTLDHLVEGVRDGHATAVLAASIFHFGEYTIAQAK
AHMAAAGLPMRL
>Mature_252_residues
MLKMRIIPCLDVKDGRVVKGVNFVGLRDAGDPVEQARLYDAAGADELCFLDITASHENRDTLLDVVGRTADQCFMPLTVG
GGVRTTEDIRKLLLAGADKVSINTAAVTRPEFVREAAETFGAQCVVVALDAKSVGPGRFEIFTHGGRKATGLDAVDWAKR
MVALGAGEILLTSMDRDGTRQGFNLPLTRAIADAVAVPVIASGGVGTLDHLVEGVRDGHATAVLAASIFHFGEYTIAQAK
AHMAAAGLPMRL

Specific function: IGPS catalyzes the conversion of PRFAR and glutamine to IGP, AICAR and glutamate. The hisF subunit catalyzes the cyclization activity that produces IGP and AICAR from PRFAR using the ammonia provided by the hisH subunit

COG id: COG0107

COG function: function code E; Imidazoleglycerol-phosphate synthase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the hisA/hisF family

Homologues:

Organism=Escherichia coli, GI1788336, Length=257, Percent_Identity=40.8560311284047, Blast_Score=199, Evalue=1e-52,
Organism=Escherichia coli, GI87082028, Length=240, Percent_Identity=30.4166666666667, Blast_Score=97, Evalue=1e-21,
Organism=Saccharomyces cerevisiae, GI6319725, Length=314, Percent_Identity=34.0764331210191, Blast_Score=148, Evalue=6e-37,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): HIS6_RHORT (Q2RNA7)

Other databases:

- EMBL:   CP000230
- RefSeq:   YP_428675.1
- ProteinModelPortal:   Q2RNA7
- SMR:   Q2RNA7
- STRING:   Q2RNA7
- GeneID:   3837050
- GenomeReviews:   CP000230_GR
- KEGG:   rru:Rru_A3594
- NMPDR:   fig|1085.1.peg.1903
- eggNOG:   COG0107
- HOGENOM:   HBG541613
- OMA:   RVVKGTN
- PhylomeDB:   Q2RNA7
- ProtClustDB:   PRK02083
- BioCyc:   RRUB269796:RRU_A3594-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01013
- InterPro:   IPR013785
- InterPro:   IPR006062
- InterPro:   IPR004651
- InterPro:   IPR011060
- Gene3D:   G3DSA:3.20.20.70
- TIGRFAMs:   TIGR00735

Pfam domain/function: PF00977 His_biosynth; SSF51366 RibP_bind_barrel

EC number: 4.1.3.-

Molecular weight: Translated: 26596; Mature: 26596

Theoretical pI: Translated: 6.25; Mature: 6.25

Prosite motif: NA

Important sites: ACT_SITE 11-11 ACT_SITE 130-130

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLKMRIIPCLDVKDGRVVKGVNFVGLRDAGDPVEQARLYDAAGADELCFLDITASHENRD
CCEEEEEEEECCCCCCEEECEEEEEECCCCCHHHHHHHHHCCCCCCEEEEEEECCCCCHH
TLLDVVGRTADQCFMPLTVGGGVRTTEDIRKLLLAGADKVSINTAAVTRPEFVREAAETF
HHHHHHCCCHHHEEEEEEECCCCCCHHHHHHHHHCCCCCEEEEHHCCCCHHHHHHHHHHC
GAQCVVVALDAKSVGPGRFEIFTHGGRKATGLDAVDWAKRMVALGAGEILLTSMDRDGTR
CCEEEEEEEECCCCCCCEEEEEECCCCCCCCCCHHHHHHHHHHHCCCCEEEEECCCCCCC
QGFNLPLTRAIADAVAVPVIASGGVGTLDHLVEGVRDGHATAVLAASIFHFGEYTIAQAK
CCCCCCHHHHHHHHHHHHEEECCCCCHHHHHHHHHCCCCHHHHHHHHHHHHCCHHHHHHH
AHMAAAGLPMRL
HHHHHCCCCCCC
>Mature Secondary Structure
MLKMRIIPCLDVKDGRVVKGVNFVGLRDAGDPVEQARLYDAAGADELCFLDITASHENRD
CCEEEEEEEECCCCCCEEECEEEEEECCCCCHHHHHHHHHCCCCCCEEEEEEECCCCCHH
TLLDVVGRTADQCFMPLTVGGGVRTTEDIRKLLLAGADKVSINTAAVTRPEFVREAAETF
HHHHHHCCCHHHEEEEEEECCCCCCHHHHHHHHHCCCCCEEEEHHCCCCHHHHHHHHHHC
GAQCVVVALDAKSVGPGRFEIFTHGGRKATGLDAVDWAKRMVALGAGEILLTSMDRDGTR
CCEEEEEEEECCCCCCCEEEEEECCCCCCCCCCHHHHHHHHHHHCCCCEEEEECCCCCCC
QGFNLPLTRAIADAVAVPVIASGGVGTLDHLVEGVRDGHATAVLAASIFHFGEYTIAQAK
CCCCCCHHHHHHHHHHHHEEECCCCCHHHHHHHHHCCCCHHHHHHHHHHHHCCHHHHHHH
AHMAAAGLPMRL
HHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Lyases; Carbon-Nitrogen Lyases; Amidine-Lyases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA