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The map label for this gene is cobQ [H]

Identifier: 83594878

GI number: 83594878

Start: 4088074

End: 4089624

Strand: Direct

Name: cobQ [H]

Synonym: Rru_A3549

Alternate gene names: 83594878

Gene position: 4088074-4089624 (Clockwise)

Preceding gene: 83594877

Following gene: 83594879

Centisome position: 93.92

GC content: 69.05

Gene sequence:

>1551_bases
GTGACCGATCCCACCGCGCCGATCGATTTTCCCGCCCTGTCGCCCAAAGCTCCACGCCGGGCCAAGGCCCTGATGATCCA
GGGCACCAGTTCCGATGTCGGCAAATCGTTGCTGGTTGCCGGCCTGTGCCGGGCCTATACGCGCCGAGGGCTGTCCGTGC
GCCCGTTCAAGGCGCAGAACATGAGCAACAACGCCGCCGTCGCCGCCGATGGCGATGACGACTCGACCACCAAAGGCGAG
ATCGGCCGGGCCCAGGCCCTGCAGGCCCGCGCCTGCAAGGTGGCGCCGTCGATCCATATGAACCCGGTGCTGCTCAAGCC
GCAGACGCTGATCGGCGCCCAGGTCGTGCTGCGCGGCCGGGTTCTTGGCAATTGTCCGGCACGCCAATACCACCTGATGA
AGCCGCAGTTGATGCCGGCGGTGATGGACAGCTTCAACCGCACCGCCGCCCTGGCCGATCTGGTGATCGTCGAGGGGGCG
GGCAGCAATGCCGAGGTCTATCTGCGGGCCTCGGACATCACCAATATGAAGCTGGCCGAGAACGCCGATCTGCCGGTGGT
GGTGGTCGGCGACATCGATCGCGGCGGGGCGCTGGCCGCCATCGTCGGCACCCACGCCCTGCTGACCGAGGACGAGCGGG
CGCGGGTGGTCGGTTATATCATCAACAAATTTCGCGGCGATCTGTCGCTGTTCGAACCGGCCTGCGCGGTGATGACCGAG
CGCACCGGCTGGCCGTGGCTGGGCGTGGTGCGCTGGTTTGAAAGCGCCGACCGCCTGCCCGCCGAAGACGCCCTGGCCCT
GGAACGCCCGGCCGAGGGGTCGGGCAAGGGCCTGCGGGTGGCGGTGCCCCAGTTGTCGCGGGTGGCCAATTTCGACGATA
TCGATCCGTTGGCCGCCGAGCCGGGCGTCGAGGTGCGCTGGATCCGCCCCGGCCATCCGATTCCGGCGGAAACCGATGTG
GTCATCCTGCCCGGCTCGAAGGTGACGCGGGCCGATCTTGACGTGCTGCGCCGCGAGGGCTGGGATATCGACATCCTGGC
CCATGTCCGCCGGGGCGGCCGGGTGGTCGGGCTGTGCGCCGGCTTCCAGATGCTGGGCAAGGTGGTGCGCGATCCCGAAG
GCATCGAAGGGCCGCCGGGCGAGACGGCCGGGCTGGGCCTGCTCGATATCGAAACGGTGATCGGCGGCGAAAAGCGCCTT
GTCGATATCGCCGTGACCGACCAACGCAGCGGCCAGCCGATCCGCGGCTATGAAATGCACATGGGCCGCACCACCGGCCC
CGGGCTTGAGCGCCCGTGGTTCCTGCTCGACGACGGCGCGGGCGGGCCGGCGCGCCGCGAAGGCGCGGTTTCGGCCGATG
GTCTGGTCAGCGGCGCCTATATCCACGGCCTGTTCGGCGGCGACGACTTCCGGAGCCACTGGCTGACCGGCCTGGGCGCG
GCGGCTTCGGGGCTGGATTTCGAGGCGCGCACCGAAACCGCGCTCGAGGCTTTGGCCGACCATCTGGAGGCCAATCTCGA
CCTCGACGCCCTGCTTGCCTTCGCCCGCTGA

Upstream 100 bases:

>100_bases
TTCCGGGGCGGGCGGACCGCCTTGACGGGCTGGAGAGACCTCGGGGAAGATCGCTCTTTTCCACCCCCCCAATCCGCCAA
GCCGTATCAAGGGCCCCGCC

Downstream 100 bases:

>100_bases
GCGGAGGGGGCGATGACCGATGATCCGACGATCGCCTTTCAGCGGCTGAAAAACCCCCACCCCCTCCGGGCGCGCGGCCT
GATGCTGCAGGGCACGGCCT

Product: cobyric acid synthase

Products: ADP; phosphate; adenosylcobyric acid; L-glutamate

Alternate protein names: NA

Number of amino acids: Translated: 516; Mature: 515

Protein sequence:

>516_residues
MTDPTAPIDFPALSPKAPRRAKALMIQGTSSDVGKSLLVAGLCRAYTRRGLSVRPFKAQNMSNNAAVAADGDDDSTTKGE
IGRAQALQARACKVAPSIHMNPVLLKPQTLIGAQVVLRGRVLGNCPARQYHLMKPQLMPAVMDSFNRTAALADLVIVEGA
GSNAEVYLRASDITNMKLAENADLPVVVVGDIDRGGALAAIVGTHALLTEDERARVVGYIINKFRGDLSLFEPACAVMTE
RTGWPWLGVVRWFESADRLPAEDALALERPAEGSGKGLRVAVPQLSRVANFDDIDPLAAEPGVEVRWIRPGHPIPAETDV
VILPGSKVTRADLDVLRREGWDIDILAHVRRGGRVVGLCAGFQMLGKVVRDPEGIEGPPGETAGLGLLDIETVIGGEKRL
VDIAVTDQRSGQPIRGYEMHMGRTTGPGLERPWFLLDDGAGGPARREGAVSADGLVSGAYIHGLFGGDDFRSHWLTGLGA
AASGLDFEARTETALEALADHLEANLDLDALLAFAR

Sequences:

>Translated_516_residues
MTDPTAPIDFPALSPKAPRRAKALMIQGTSSDVGKSLLVAGLCRAYTRRGLSVRPFKAQNMSNNAAVAADGDDDSTTKGE
IGRAQALQARACKVAPSIHMNPVLLKPQTLIGAQVVLRGRVLGNCPARQYHLMKPQLMPAVMDSFNRTAALADLVIVEGA
GSNAEVYLRASDITNMKLAENADLPVVVVGDIDRGGALAAIVGTHALLTEDERARVVGYIINKFRGDLSLFEPACAVMTE
RTGWPWLGVVRWFESADRLPAEDALALERPAEGSGKGLRVAVPQLSRVANFDDIDPLAAEPGVEVRWIRPGHPIPAETDV
VILPGSKVTRADLDVLRREGWDIDILAHVRRGGRVVGLCAGFQMLGKVVRDPEGIEGPPGETAGLGLLDIETVIGGEKRL
VDIAVTDQRSGQPIRGYEMHMGRTTGPGLERPWFLLDDGAGGPARREGAVSADGLVSGAYIHGLFGGDDFRSHWLTGLGA
AASGLDFEARTETALEALADHLEANLDLDALLAFAR
>Mature_515_residues
TDPTAPIDFPALSPKAPRRAKALMIQGTSSDVGKSLLVAGLCRAYTRRGLSVRPFKAQNMSNNAAVAADGDDDSTTKGEI
GRAQALQARACKVAPSIHMNPVLLKPQTLIGAQVVLRGRVLGNCPARQYHLMKPQLMPAVMDSFNRTAALADLVIVEGAG
SNAEVYLRASDITNMKLAENADLPVVVVGDIDRGGALAAIVGTHALLTEDERARVVGYIINKFRGDLSLFEPACAVMTER
TGWPWLGVVRWFESADRLPAEDALALERPAEGSGKGLRVAVPQLSRVANFDDIDPLAAEPGVEVRWIRPGHPIPAETDVV
ILPGSKVTRADLDVLRREGWDIDILAHVRRGGRVVGLCAGFQMLGKVVRDPEGIEGPPGETAGLGLLDIETVIGGEKRLV
DIAVTDQRSGQPIRGYEMHMGRTTGPGLERPWFLLDDGAGGPARREGAVSADGLVSGAYIHGLFGGDDFRSHWLTGLGAA
ASGLDFEARTETALEALADHLEANLDLDALLAFAR

Specific function: Catalyzes amidations at positions B, D, E, and G on adenosylcobyrinic A,C-diamide. NH(2) groups are provided by glutamine, and one molecule of ATP is hydrogenolyzed for each amidation [H]

COG id: COG1492

COG function: function code H; Cobyric acid synthase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 GATase cobBQ-type domain [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002586
- InterPro:   IPR017929
- InterPro:   IPR004459
- InterPro:   IPR011698 [H]

Pfam domain/function: PF01656 CbiA; PF07685 GATase_3 [H]

EC number: 6.3.5.10

Molecular weight: Translated: 54942; Mature: 54811

Theoretical pI: Translated: 5.53; Mature: 5.53

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDPTAPIDFPALSPKAPRRAKALMIQGTSSDVGKSLLVAGLCRAYTRRGLSVRPFKAQN
CCCCCCCCCCCCCCCCCCCCEEEEEEECCCCHHHHHHHHHHHHHHHHHCCCEECCEECCC
MSNNAAVAADGDDDSTTKGEIGRAQALQARACKVAPSIHMNPVLLKPQTLIGAQVVLRGR
CCCCEEEEECCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCEEEECCHHHHHHHHEEECH
VLGNCPARQYHLMKPQLMPAVMDSFNRTAALADLVIVEGAGSNAEVYLRASDITNMKLAE
HHCCCCCCCEECCCCHHHHHHHHHCCHHHHEEEEEEEECCCCCCEEEEEECCCCCEEECC
NADLPVVVVGDIDRGGALAAIVGTHALLTEDERARVVGYIINKFRGDLSLFEPACAVMTE
CCCCCEEEEECCCCCCEEEEEECCHHEECCCCHHHHHHHHHHHHCCCCHHHCCHHHHHCC
RTGWPWLGVVRWFESADRLPAEDALALERPAEGSGKGLRVAVPQLSRVANFDDIDPLAAE
CCCCCHHHHHHHHHHHCCCCCCCCCEECCCCCCCCCCEEEECCHHHHHCCCCCCCCCCCC
PGVEVRWIRPGHPIPAETDVVILPGSKVTRADLDVLRREGWDIDILAHVRRGGRVVGLCA
CCCEEEEECCCCCCCCCCCEEEECCCCCCHHHHHHHHHCCCCEEEHHHHHCCCEEEEEHH
GFQMLGKVVRDPEGIEGPPGETAGLGLLDIETVIGGEKRLVDIAVTDQRSGQPIRGYEMH
HHHHHHHHHCCCCCCCCCCCCCCCCEEEEEHHHHCCCEEEEEEEEECCCCCCCCCCEEEE
MGRTTGPGLERPWFLLDDGAGGPARREGAVSADGLVSGAYIHGLFGGDDFRSHWLTGLGA
CCCCCCCCCCCCEEEEECCCCCCCCCCCCCCCCCCEECEEEEEECCCCHHHHHHHHHCCC
AASGLDFEARTETALEALADHLEANLDLDALLAFAR
CCCCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHCC
>Mature Secondary Structure 
TDPTAPIDFPALSPKAPRRAKALMIQGTSSDVGKSLLVAGLCRAYTRRGLSVRPFKAQN
CCCCCCCCCCCCCCCCCCCEEEEEEECCCCHHHHHHHHHHHHHHHHHCCCEECCEECCC
MSNNAAVAADGDDDSTTKGEIGRAQALQARACKVAPSIHMNPVLLKPQTLIGAQVVLRGR
CCCCEEEEECCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCEEEECCHHHHHHHHEEECH
VLGNCPARQYHLMKPQLMPAVMDSFNRTAALADLVIVEGAGSNAEVYLRASDITNMKLAE
HHCCCCCCCEECCCCHHHHHHHHHCCHHHHEEEEEEEECCCCCCEEEEEECCCCCEEECC
NADLPVVVVGDIDRGGALAAIVGTHALLTEDERARVVGYIINKFRGDLSLFEPACAVMTE
CCCCCEEEEECCCCCCEEEEEECCHHEECCCCHHHHHHHHHHHHCCCCHHHCCHHHHHCC
RTGWPWLGVVRWFESADRLPAEDALALERPAEGSGKGLRVAVPQLSRVANFDDIDPLAAE
CCCCCHHHHHHHHHHHCCCCCCCCCEECCCCCCCCCCEEEECCHHHHHCCCCCCCCCCCC
PGVEVRWIRPGHPIPAETDVVILPGSKVTRADLDVLRREGWDIDILAHVRRGGRVVGLCA
CCCEEEEECCCCCCCCCCCEEEECCCCCCHHHHHHHHHCCCCEEEHHHHHCCCEEEEEHH
GFQMLGKVVRDPEGIEGPPGETAGLGLLDIETVIGGEKRLVDIAVTDQRSGQPIRGYEMH
HHHHHHHHHCCCCCCCCCCCCCCCCEEEEEHHHHCCCEEEEEEEEECCCCCCCCCCEEEE
MGRTTGPGLERPWFLLDDGAGGPARREGAVSADGLVSGAYIHGLFGGDDFRSHWLTGLGA
CCCCCCCCCCCCEEEEECCCCCCCCCCCCCCCCCCEECEEEEEECCCCHHHHHHHHHCCC
AASGLDFEARTETALEALADHLEANLDLDALLAFAR
CCCCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; adenosylcobyrinic acid a,c-diamide; L-glutamine; H2O

Specific reaction: 4 ATP + adenosylcobyrinic acid a,c-diamide + 4 L-glutamine + 4 H2O = 4 ADP + 4 phosphate + adenosylcobyric acid + 4 L-glutamate

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7635831; 9256491 [H]