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The map label for this gene is emrB [H]

Identifier: 83594812

GI number: 83594812

Start: 4007437

End: 4009071

Strand: Direct

Name: emrB [H]

Synonym: Rru_A3483

Alternate gene names: 83594812

Gene position: 4007437-4009071 (Clockwise)

Preceding gene: 83594811

Following gene: 83594813

Centisome position: 92.07

GC content: 61.59

Gene sequence:

>1635_bases
ATGTCCGGGCCCAGTAGTCCTCCCGCTCCGCCCGCCGCTCCGCCGATGTCGACGGGGCGGTCAACCATTTATATCGCGGC
TTCGGTCCTGCTCTGGATGACCCAGGGCCTCGGCATGAACCTGATCGCCGTGAATACCTTGCAGATCCAGGGGTCTCTGG
GGGCGACCTTGACGGAAACATCCTGGCTGATCGCGGCCTATATGGCGCCCAATGTCAGCTTGACGATCTTGCTGACCAAG
ATCCGCATTCAATTCGGCCTGCGCCGGTTTGCCGAACTCAGCTTGATTATTTTCGCCGTCACCTCGCTGCTTCATCTGGT
CATCTTTGATTTGTGGACGGCCCTGCCCATTCGGTTTCTGGCCGGCATGGCGGCCGCGCCGGTTTCCACCTTGGGCTTTC
TTTACATGCTGGAGGCCTTTCCCCCGGCCAAGAAACTGACCTGGGCGGTTAGCCTGTCGCTGACCTGTTCGGCGGCGACG
CCGATGGTGGCCCGGGTGATCTCGCCGGTTCTGCTTGATCTCGGCCAATGGCAACAGCTTTACACCCTGGAACTCGGTCT
GGCCCTGCTTGCCTTCTCCGTCGTTTTCCTGCTGCCCCTAACCCCCTTGCCTTATGCCAAGGTCCTGCATTGGCGCGACT
TTATTACTTATCCCTTGGTTGCCATCGGCTTTGGCCTGCTGGCGGTGGTGCTGGCTTTGGGGCGCTTTTACTGGTGGTTC
GAGGCGCCGTGGATTGGCATCTGCTTGGCTATCGCCGCGCTGTGCATCGGCGGCGCGGCGGCCGTGGAACTGCACCGCGA
CACGCCGCTGCTGAATATCCGTTGGTTGACCAGCCCCGAAATCGCCACCCTCGCCCTGACCTTGCTGATCTTTCGCATCG
TGCTGGCCGAACAGACATCGGGCGCCATCGGCCTGTTCCAGACCCTTGGTCTGCTCAACGAGCAAAGCAGGACTCTTTAT
CTCATCCTTCTGGCGGCGACCCTGGCGGGTGGACTGATCTGCGGCCTCACCCTGACCCTGGCCCGCGTGCCGCTGTTTCA
TGGGATCGCTTTGATCTGTATCGCCGGCGGCGCGGTCATGGATGGCCATGCCACCGCGCTCACCCGGCCCGAGACCATGT
ATTTCAGTCAGGCCCTGATCGCCTTCGGCGGTGCCCTGTTCCTGCCCCCGGCCATGCATTCGGGGCTGGTCAAGACTTTC
AAGCAAGGGCCGATGTTCATCACCAGTTTCATCGCCGTCTTTTTGTTTACCCAAAGCATCGGCGGGCTGATGGGCTCGGC
GTTGTTTGGCAGCCTGGTTACCCTGCGTGAGAAGGTCCATTCCGCCCATCTGGTCGAGCAGATCCAACTGACCAATCCGA
TCGTTGCCGATCGTCTTCATACCTTGGCCGGGGCCTATCATCCCCTCCTGAGCGATCCGCAGCAGCTTATGACCGAAGGC
ATGGCCCTGTTGTCCCAGCAGGTCACGCGTGAGGCCACCGTTCTTGCCTATAACGATGCCTTCCTTCTGCTTGCCGCCAT
CGCCAGCGCCTTCCTGGCGGCCCTTGGTCTGCATATGGCCTATCGCCGCCTGCGCGGCCGTCTGGACCGTTCACGCGGCC
CGGACGCCCTGATCCCCCAATCGAGACCCACCTGA

Upstream 100 bases:

>100_bases
AAGATCGCGCTGGCGGTGCTGCGTCAGGTGGTCAGCAACGCGGAAGCGGCCCGCTCCGATGACGCCGACCTCACCGCGCC
CCCGGCGGATGGCAAAGACC

Downstream 100 bases:

>100_bases
ACCATGTCAAAGGTCAAACATTTTTTCCCTGCCGGGCTCGCGCTGTCCATCGGCCTCATCGGTGCCCTGCTCGTTCTCTA
TGCGTGGCACCTGCCGCCCT

Product: major facilitator transporter

Products: Proton [Cytoplasm]; multidrug [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 544; Mature: 543

Protein sequence:

>544_residues
MSGPSSPPAPPAAPPMSTGRSTIYIAASVLLWMTQGLGMNLIAVNTLQIQGSLGATLTETSWLIAAYMAPNVSLTILLTK
IRIQFGLRRFAELSLIIFAVTSLLHLVIFDLWTALPIRFLAGMAAAPVSTLGFLYMLEAFPPAKKLTWAVSLSLTCSAAT
PMVARVISPVLLDLGQWQQLYTLELGLALLAFSVVFLLPLTPLPYAKVLHWRDFITYPLVAIGFGLLAVVLALGRFYWWF
EAPWIGICLAIAALCIGGAAAVELHRDTPLLNIRWLTSPEIATLALTLLIFRIVLAEQTSGAIGLFQTLGLLNEQSRTLY
LILLAATLAGGLICGLTLTLARVPLFHGIALICIAGGAVMDGHATALTRPETMYFSQALIAFGGALFLPPAMHSGLVKTF
KQGPMFITSFIAVFLFTQSIGGLMGSALFGSLVTLREKVHSAHLVEQIQLTNPIVADRLHTLAGAYHPLLSDPQQLMTEG
MALLSQQVTREATVLAYNDAFLLLAAIASAFLAALGLHMAYRRLRGRLDRSRGPDALIPQSRPT

Sequences:

>Translated_544_residues
MSGPSSPPAPPAAPPMSTGRSTIYIAASVLLWMTQGLGMNLIAVNTLQIQGSLGATLTETSWLIAAYMAPNVSLTILLTK
IRIQFGLRRFAELSLIIFAVTSLLHLVIFDLWTALPIRFLAGMAAAPVSTLGFLYMLEAFPPAKKLTWAVSLSLTCSAAT
PMVARVISPVLLDLGQWQQLYTLELGLALLAFSVVFLLPLTPLPYAKVLHWRDFITYPLVAIGFGLLAVVLALGRFYWWF
EAPWIGICLAIAALCIGGAAAVELHRDTPLLNIRWLTSPEIATLALTLLIFRIVLAEQTSGAIGLFQTLGLLNEQSRTLY
LILLAATLAGGLICGLTLTLARVPLFHGIALICIAGGAVMDGHATALTRPETMYFSQALIAFGGALFLPPAMHSGLVKTF
KQGPMFITSFIAVFLFTQSIGGLMGSALFGSLVTLREKVHSAHLVEQIQLTNPIVADRLHTLAGAYHPLLSDPQQLMTEG
MALLSQQVTREATVLAYNDAFLLLAAIASAFLAALGLHMAYRRLRGRLDRSRGPDALIPQSRPT
>Mature_543_residues
SGPSSPPAPPAAPPMSTGRSTIYIAASVLLWMTQGLGMNLIAVNTLQIQGSLGATLTETSWLIAAYMAPNVSLTILLTKI
RIQFGLRRFAELSLIIFAVTSLLHLVIFDLWTALPIRFLAGMAAAPVSTLGFLYMLEAFPPAKKLTWAVSLSLTCSAATP
MVARVISPVLLDLGQWQQLYTLELGLALLAFSVVFLLPLTPLPYAKVLHWRDFITYPLVAIGFGLLAVVLALGRFYWWFE
APWIGICLAIAALCIGGAAAVELHRDTPLLNIRWLTSPEIATLALTLLIFRIVLAEQTSGAIGLFQTLGLLNEQSRTLYL
ILLAATLAGGLICGLTLTLARVPLFHGIALICIAGGAVMDGHATALTRPETMYFSQALIAFGGALFLPPAMHSGLVKTFK
QGPMFITSFIAVFLFTQSIGGLMGSALFGSLVTLREKVHSAHLVEQIQLTNPIVADRLHTLAGAYHPLLSDPQQLMTEGM
ALLSQQVTREATVLAYNDAFLLLAAIASAFLAALGLHMAYRRLRGRLDRSRGPDALIPQSRPT

Specific function: Translocase that confers resistance to substances of high hydrophobicity [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. EmrB family [H]

Homologues:

Organism=Escherichia coli, GI1789042, Length=515, Percent_Identity=24.6601941747573, Blast_Score=69, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004638
- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 58549; Mature: 58417

Theoretical pI: Translated: 9.30; Mature: 9.30

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSGPSSPPAPPAAPPMSTGRSTIYIAASVLLWMTQGLGMNLIAVNTLQIQGSLGATLTET
CCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCEEEEEEEEEEECCCCCCCHHH
SWLIAAYMAPNVSLTILLTKIRIQFGLRRFAELSLIIFAVTSLLHLVIFDLWTALPIRFL
HHHHHHHHCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AGMAAAPVSTLGFLYMLEAFPPAKKLTWAVSLSLTCSAATPMVARVISPVLLDLGQWQQL
HHHHHCHHHHHHHHHHHHHCCCHHHEEEEEEEEEEECCCHHHHHHHHHHHHHHHHCHHHH
YTLELGLALLAFSVVFLLPLTPLPYAKVLHWRDFITYPLVAIGFGLLAVVLALGRFYWWF
HHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EAPWIGICLAIAALCIGGAAAVELHRDTPLLNIRWLTSPEIATLALTLLIFRIVLAEQTS
CCCHHHHHHHHHHHHHCCHHEEEEECCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHCCC
GAIGLFQTLGLLNEQSRTLYLILLAATLAGGLICGLTLTLARVPLFHGIALICIAGGAVM
CHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEE
DGHATALTRPETMYFSQALIAFGGALFLPPAMHSGLVKTFKQGPMFITSFIAVFLFTQSI
CCCCHHCCCCHHHHHHHHHHHHCCHHHCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHH
GGLMGSALFGSLVTLREKVHSAHLVEQIQLTNPIVADRLHTLAGAYHPLLSDPQQLMTEG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCHHCCCHHHHHHHH
MALLSQQVTREATVLAYNDAFLLLAAIASAFLAALGLHMAYRRLRGRLDRSRGPDALIPQ
HHHHHHHHHHHHEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCC
SRPT
CCCC
>Mature Secondary Structure 
SGPSSPPAPPAAPPMSTGRSTIYIAASVLLWMTQGLGMNLIAVNTLQIQGSLGATLTET
CCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCEEEEEEEEEEECCCCCCCHHH
SWLIAAYMAPNVSLTILLTKIRIQFGLRRFAELSLIIFAVTSLLHLVIFDLWTALPIRFL
HHHHHHHHCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AGMAAAPVSTLGFLYMLEAFPPAKKLTWAVSLSLTCSAATPMVARVISPVLLDLGQWQQL
HHHHHCHHHHHHHHHHHHHCCCHHHEEEEEEEEEEECCCHHHHHHHHHHHHHHHHCHHHH
YTLELGLALLAFSVVFLLPLTPLPYAKVLHWRDFITYPLVAIGFGLLAVVLALGRFYWWF
HHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EAPWIGICLAIAALCIGGAAAVELHRDTPLLNIRWLTSPEIATLALTLLIFRIVLAEQTS
CCCHHHHHHHHHHHHHCCHHEEEEECCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHCCC
GAIGLFQTLGLLNEQSRTLYLILLAATLAGGLICGLTLTLARVPLFHGIALICIAGGAVM
CHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEE
DGHATALTRPETMYFSQALIAFGGALFLPPAMHSGLVKTFKQGPMFITSFIAVFLFTQSI
CCCCHHCCCCHHHHHHHHHHHHCCHHHCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHH
GGLMGSALFGSLVTLREKVHSAHLVEQIQLTNPIVADRLHTLAGAYHPLLSDPQQLMTEG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCHHCCCHHHHHHHH
MALLSQQVTREATVLAYNDAFLLLAAIASAFLAALGLHMAYRRLRGRLDRSRGPDALIPQ
HHHHHHHHHHHHEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCC
SRPT
CCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; multidrug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + multidrug [Cytoplasm] = Proton [Cytoplasm] + multidrug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]