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The map label for this gene is emrA [H]

Identifier: 83594813

GI number: 83594813

Start: 4009075

End: 4010139

Strand: Direct

Name: emrA [H]

Synonym: Rru_A3484

Alternate gene names: 83594813

Gene position: 4009075-4010139 (Clockwise)

Preceding gene: 83594812

Following gene: 83594815

Centisome position: 92.1

GC content: 64.6

Gene sequence:

>1065_bases
ATGTCAAAGGTCAAACATTTTTTCCCTGCCGGGCTCGCGCTGTCCATCGGCCTCATCGGTGCCCTGCTCGTTCTCTATGC
GTGGCACCTGCCGCCCTTCCGCAGCGCCGTCGAAACGACCGACAACGCCTATGTGCGCGCTCAGGTCACCATGATCAGTC
CGCAAAGCGCCGGCTATGTGACCGCGGTCACCGTCCATGATTTCCAGACGGTCAAGGCCGGCACGCCGCTGTTTCAGATC
GATGACCGGATCTATGCGCAAAAGCTGAAGCAGGCCCAGGCGACCTTAGAAATGAAACGCGCCGCCCTGGAGAATTCCGA
GCAGAGCGAGCGGGCGGCGCAGGCGCGGATCCGCTCCAGCGAGGCCGAGATCGCCAGCGCCATCGCCGCCCTGGATGTCG
CCCGGTCCACCGCCCGCCGCACCGAGGCTTTGCTGGCGCGCGGCGTCTCGACGCAAAGCTCCGCCGATCAGGCCCGCTCC
ACCCTGGCCCAGGCCGAAGCCCTGGTACACAAAAGCCAAGCCGCCCTTGACGTTTCCCGCGAGGATCTGCGCTCGATCCG
CGTTAATCGGGACTCGCTTCAGGCCGATATCGCCAATGCCCAGGCGGCGGTCGAACTGGCGACGATTGATTTGCACAACA
CCAGCATCGTCGCGCCAATCGATGGCACGCTGGGCGAGATCGGGGTGCGGTTGGGGCAGTATGTTTCGGCCGGAACCCAG
CTCGCCGCCCTGGTGCCCGCAAGACGCTGGGTCATCGCCAATTTCAAGGAAACCCAGTTGGCCGGCATCCAGATCGGTCA
GGCGGTGAGCTTTCGCGTCGATGCCCTGCAGGGGACGGCCCTGGCCGGGCATGTCGAAGCCTTTTCGCCCGCCACGGGCT
CAGAGTTTACCGTGCTGAAATCCGACAACGCCACGGGCAACTTCACCAAGGTCACCCAGCGGCTTCCCGTTCGCATCGCC
ATCGATCCCGATCAGGCGCAGGCCGCCCTGTTGGCGCCGGGAATGTCGGTGGTGGTGCGCATCGATACCGCCCCATCCGA
GAGGGACGGAAAGCACCCGTCTTAA

Upstream 100 bases:

>100_bases
GGCCCTTGGTCTGCATATGGCCTATCGCCGCCTGCGCGGCCGTCTGGACCGTTCACGCGGCCCGGACGCCCTGATCCCCC
AATCGAGACCCACCTGAACC

Downstream 100 bases:

>100_bases
AGCAGATCCCCGGGCGATCTAGGGGGAGTGCTGATCCAGGCGCTATTTATTGGCGCGCAGGGTTTCCAGGCGGAAGGTGG
GGGGCAGGGGTTCCTTGCGG

Product: secretion protein HlyD

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 354; Mature: 353

Protein sequence:

>354_residues
MSKVKHFFPAGLALSIGLIGALLVLYAWHLPPFRSAVETTDNAYVRAQVTMISPQSAGYVTAVTVHDFQTVKAGTPLFQI
DDRIYAQKLKQAQATLEMKRAALENSEQSERAAQARIRSSEAEIASAIAALDVARSTARRTEALLARGVSTQSSADQARS
TLAQAEALVHKSQAALDVSREDLRSIRVNRDSLQADIANAQAAVELATIDLHNTSIVAPIDGTLGEIGVRLGQYVSAGTQ
LAALVPARRWVIANFKETQLAGIQIGQAVSFRVDALQGTALAGHVEAFSPATGSEFTVLKSDNATGNFTKVTQRLPVRIA
IDPDQAQAALLAPGMSVVVRIDTAPSERDGKHPS

Sequences:

>Translated_354_residues
MSKVKHFFPAGLALSIGLIGALLVLYAWHLPPFRSAVETTDNAYVRAQVTMISPQSAGYVTAVTVHDFQTVKAGTPLFQI
DDRIYAQKLKQAQATLEMKRAALENSEQSERAAQARIRSSEAEIASAIAALDVARSTARRTEALLARGVSTQSSADQARS
TLAQAEALVHKSQAALDVSREDLRSIRVNRDSLQADIANAQAAVELATIDLHNTSIVAPIDGTLGEIGVRLGQYVSAGTQ
LAALVPARRWVIANFKETQLAGIQIGQAVSFRVDALQGTALAGHVEAFSPATGSEFTVLKSDNATGNFTKVTQRLPVRIA
IDPDQAQAALLAPGMSVVVRIDTAPSERDGKHPS
>Mature_353_residues
SKVKHFFPAGLALSIGLIGALLVLYAWHLPPFRSAVETTDNAYVRAQVTMISPQSAGYVTAVTVHDFQTVKAGTPLFQID
DRIYAQKLKQAQATLEMKRAALENSEQSERAAQARIRSSEAEIASAIAALDVARSTARRTEALLARGVSTQSSADQARST
LAQAEALVHKSQAALDVSREDLRSIRVNRDSLQADIANAQAAVELATIDLHNTSIVAPIDGTLGEIGVRLGQYVSAGTQL
AALVPARRWVIANFKETQLAGIQIGQAVSFRVDALQGTALAGHVEAFSPATGSEFTVLKSDNATGNFTKVTQRLPVRIAI
DPDQAQAALLAPGMSVVVRIDTAPSERDGKHPS

Specific function: The emr locus confers resistance to substances of high hydrophobicity. EmrA probably participate in a transport system to extrude toxins and drugs from the cell [H]

COG id: COG1566

COG function: function code V; Multidrug resistance efflux pump

Gene ontology:

Cell location: Cell inner membrane; Single-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the membrane fusion protein (MFP) (TC 8.A.1) family [H]

Homologues:

Organism=Escherichia coli, GI1788711, Length=343, Percent_Identity=30.3206997084548, Blast_Score=136, Evalue=2e-33,
Organism=Escherichia coli, GI1789041, Length=346, Percent_Identity=31.5028901734104, Blast_Score=119, Evalue=2e-28,
Organism=Escherichia coli, GI1790519, Length=304, Percent_Identity=31.9078947368421, Blast_Score=116, Evalue=2e-27,
Organism=Escherichia coli, GI1789637, Length=363, Percent_Identity=25.068870523416, Blast_Score=75, Evalue=7e-15,
Organism=Escherichia coli, GI87081951, Length=328, Percent_Identity=23.780487804878, Blast_Score=74, Evalue=2e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005694
- InterPro:   IPR006143 [H]

Pfam domain/function: PF00529 HlyD [H]

EC number: NA

Molecular weight: Translated: 37682; Mature: 37551

Theoretical pI: Translated: 8.85; Mature: 8.85

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
1.1 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
0.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKVKHFFPAGLALSIGLIGALLVLYAWHLPPFRSAVETTDNAYVRAQVTMISPQSAGYV
CCCCCHHCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHCCCCEEEEEEEEEECCCCCCEE
TAVTVHDFQTVKAGTPLFQIDDRIYAQKLKQAQATLEMKRAALENSEQSERAAQARIRSS
EEEEEECCEECCCCCCEEEECHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHH
EAEIASAIAALDVARSTARRTEALLARGVSTQSSADQARSTLAQAEALVHKSQAALDVSR
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCH
EDLRSIRVNRDSLQADIANAQAAVELATIDLHNTSIVAPIDGTLGEIGVRLGQYVSAGTQ
HHHHHHHCCHHHHHHHHHCCCEEEEEEEEEECCCEEEEECCCCHHHHHHHHHHHHHCCCH
LAALVPARRWVIANFKETQLAGIQIGQAVSFRVDALQGTALAGHVEAFSPATGSEFTVLK
HHHHHCCCCEEEECCCCCEECCEEECCEEEEEEECCCCCEEECCHHHCCCCCCCEEEEEE
SDNATGNFTKVTQRLPVRIAIDPDQAQAALLAPGMSVVVRIDTAPSERDGKHPS
CCCCCCCHHHHHHCCCEEEEECCCCHHHEEECCCCEEEEEEECCCCCCCCCCCC
>Mature Secondary Structure 
SKVKHFFPAGLALSIGLIGALLVLYAWHLPPFRSAVETTDNAYVRAQVTMISPQSAGYV
CCCCHHCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHCCCCEEEEEEEEEECCCCCCEE
TAVTVHDFQTVKAGTPLFQIDDRIYAQKLKQAQATLEMKRAALENSEQSERAAQARIRSS
EEEEEECCEECCCCCCEEEECHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHH
EAEIASAIAALDVARSTARRTEALLARGVSTQSSADQARSTLAQAEALVHKSQAALDVSR
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCH
EDLRSIRVNRDSLQADIANAQAAVELATIDLHNTSIVAPIDGTLGEIGVRLGQYVSAGTQ
HHHHHHHCCHHHHHHHHHCCCEEEEEEEEEECCCEEEEECCCCHHHHHHHHHHHHHCCCH
LAALVPARRWVIANFKETQLAGIQIGQAVSFRVDALQGTALAGHVEAFSPATGSEFTVLK
HHHHHCCCCEEEECCCCCEECCEEECCEEEEEEECCCCCEEECCHHHCCCCCCCEEEEEE
SDNATGNFTKVTQRLPVRIAIDPDQAQAALLAPGMSVVVRIDTAPSERDGKHPS
CCCCCCCHHHHHHCCCEEEEECCCCHHHEEECCCCEEEEEEECCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]