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The map label for this gene is stp [H]

Identifier: 83594290

GI number: 83594290

Start: 3409475

End: 3410995

Strand: Direct

Name: stp [H]

Synonym: Rru_A2959

Alternate gene names: 83594290

Gene position: 3409475-3410995 (Clockwise)

Preceding gene: 83594288

Following gene: 83594291

Centisome position: 78.33

GC content: 65.94

Gene sequence:

>1521_bases
ATGCTTCATTCCACGTCGTCCAGGAATCAGGTCGCCCTGTCGGCTGTCTGCCTGTCTGCCTTGATGTTCGGATTGGAGAT
ATCAAGCGTCCCGACCATCCTGCCGACGCTGGAACAGCTTTTTTCGGCTGATTTCCGGCAACTGCAGTGGGTGATGAACG
CCTACACCACCGCCATGACCGCCTGTCTGATGGCCATGGGGGCCTTGGCCGACCGCTTTGGCCGCAAGCGGGTCTTTCTG
GCGGGACAGGTTGTGTTCGGCGTCGCGTCGCTGGCTTGCGGGCTGGCCGACAGCCCATCGATGCTGATCGGCGCCCGCGC
GCTTCAGGGCGCGGCGGCGGCGGCGTTGCTCACCTGTCAGATCGCGGTGTTGTCCCAGCAATTCCGCGACGGACGGGAAC
GAGCGCTTGCTTTCGGCTGGTGGGGGGTGACCTTCGGCGTCGGCCTGGGATTCGGGCCGTTGATCGGCGGGCTGACGGCG
GTGGTTCTGGGATGGGAATGGGTGTTCCTGGTCCATGTGATCCTGGCGGCGGTCGCGGTGATCCTGATCCAGGTGGGGGT
CGAGGAAAGCACCGATCCGCACGCCCTGCGCCTTGACGTCGCGGGGATGGTGACATTGTCGCTGGCCGTATTCTGCCTGG
TCTATCTGATCACGCGCGGGCGTATGCCGGGGCCCGGAGACATCTCCGGGCTAGCGCTTGCCGCACTGGGCGTGGCCTGT
TTCAGCGCGTTCGTGGTGGTCGAAACGCGGGTGGCCCGGCCGATGTTCGACTTCACCGCTTTCCGCACCACGAATTTCTC
GGGCGCGCTGCTGGCGTCAGCGGGCATGAACTTCAGCTTCTGGCCGTTCATCATCTACCTGCCGGTCTATTTCCAGGGCG
TCCTGGGTCTGGACAGCCTGGACGCCGGGCTGTGCCTGCTGGCCTATACCTTGCCGCCGCTGCTGGCGCCGCCGATTGCC
GAGCGGTTATTGCTGCATTTCGGACCACAGGTGGTCATTCCCCTGGGGCTGGTGATCATCGGGTGCGGTTTCGTCCTGAT
GCGGGCGGCGGCCCTTGGGGGAGATGCAAGCTGGGTGGACATGCTGCCCGGCTGCATCCTTGCCGGAAGCGGCCTGGGGC
TGGCCAACACCACGGCGACCAACACCGCCTCGGCCGCCCTGCCGCCGGAACGGGCCGGCATGGCCTCGGGCATGAACATG
AGCGCCAGCATGATCGCCCTGGCGATCAACATCGCCCTAATGGGTTTCATCCTGCTGCAAGGAACCCAGGCAGGGCTGGA
ACGGCTGTTGCCCGCCGCGACCGGGGACGAGTTGTCACTGCTGGCGGAAAGCGTCGCCGCCGGGGGGGAGGCGGTGGCGG
CCGGGGCCGGCCTGCCGATCGCGCTTGTCCGCCAGTCCCTGGTTCACGGCTTCGACTGGGTGATGTTGTACGGTGCGGGC
GGCGTCTGGCTGTTCGCGGCGATCTGCAAGCTGGTCTTCGGTTCAGCAAAACGGAGCGGTCCGGCACCATGCGACAGGTA
A

Upstream 100 bases:

>100_bases
AAATGTGTTTGCGCGTATAAAATGGTTAATTAAACTTAGACTCGCAACGGACAAAATTCCGCGGAACAAATTTTGACATT
CACAAGGGTGAATTTCCGCC

Downstream 100 bases:

>100_bases
CGGCCCAGGGGGCTGGCGGCAAGCCTGCCCTGAAAGGGGCACCTTATGCGGCCCCCTGGCGAGAGGATCGCGCGGGGGCG
GCTTTCTTATCCCGGTTGTG

Product: major facilitator transporter

Products: NA

Alternate protein names: Spectinomycin tetracycline efflux pump [H]

Number of amino acids: Translated: 506; Mature: 506

Protein sequence:

>506_residues
MLHSTSSRNQVALSAVCLSALMFGLEISSVPTILPTLEQLFSADFRQLQWVMNAYTTAMTACLMAMGALADRFGRKRVFL
AGQVVFGVASLACGLADSPSMLIGARALQGAAAAALLTCQIAVLSQQFRDGRERALAFGWWGVTFGVGLGFGPLIGGLTA
VVLGWEWVFLVHVILAAVAVILIQVGVEESTDPHALRLDVAGMVTLSLAVFCLVYLITRGRMPGPGDISGLALAALGVAC
FSAFVVVETRVARPMFDFTAFRTTNFSGALLASAGMNFSFWPFIIYLPVYFQGVLGLDSLDAGLCLLAYTLPPLLAPPIA
ERLLLHFGPQVVIPLGLVIIGCGFVLMRAAALGGDASWVDMLPGCILAGSGLGLANTTATNTASAALPPERAGMASGMNM
SASMIALAINIALMGFILLQGTQAGLERLLPAATGDELSLLAESVAAGGEAVAAGAGLPIALVRQSLVHGFDWVMLYGAG
GVWLFAAICKLVFGSAKRSGPAPCDR

Sequences:

>Translated_506_residues
MLHSTSSRNQVALSAVCLSALMFGLEISSVPTILPTLEQLFSADFRQLQWVMNAYTTAMTACLMAMGALADRFGRKRVFL
AGQVVFGVASLACGLADSPSMLIGARALQGAAAAALLTCQIAVLSQQFRDGRERALAFGWWGVTFGVGLGFGPLIGGLTA
VVLGWEWVFLVHVILAAVAVILIQVGVEESTDPHALRLDVAGMVTLSLAVFCLVYLITRGRMPGPGDISGLALAALGVAC
FSAFVVVETRVARPMFDFTAFRTTNFSGALLASAGMNFSFWPFIIYLPVYFQGVLGLDSLDAGLCLLAYTLPPLLAPPIA
ERLLLHFGPQVVIPLGLVIIGCGFVLMRAAALGGDASWVDMLPGCILAGSGLGLANTTATNTASAALPPERAGMASGMNM
SASMIALAINIALMGFILLQGTQAGLERLLPAATGDELSLLAESVAAGGEAVAAGAGLPIALVRQSLVHGFDWVMLYGAG
GVWLFAAICKLVFGSAKRSGPAPCDR
>Mature_506_residues
MLHSTSSRNQVALSAVCLSALMFGLEISSVPTILPTLEQLFSADFRQLQWVMNAYTTAMTACLMAMGALADRFGRKRVFL
AGQVVFGVASLACGLADSPSMLIGARALQGAAAAALLTCQIAVLSQQFRDGRERALAFGWWGVTFGVGLGFGPLIGGLTA
VVLGWEWVFLVHVILAAVAVILIQVGVEESTDPHALRLDVAGMVTLSLAVFCLVYLITRGRMPGPGDISGLALAALGVAC
FSAFVVVETRVARPMFDFTAFRTTNFSGALLASAGMNFSFWPFIIYLPVYFQGVLGLDSLDAGLCLLAYTLPPLLAPPIA
ERLLLHFGPQVVIPLGLVIIGCGFVLMRAAALGGDASWVDMLPGCILAGSGLGLANTTATNTASAALPPERAGMASGMNM
SASMIALAINIALMGFILLQGTQAGLERLLPAATGDELSLLAESVAAGGEAVAAGAGLPIALVRQSLVHGFDWVMLYGAG
GVWLFAAICKLVFGSAKRSGPAPCDR

Specific function: Contributes to spectinomycin and tetracycline resistance [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. EmrB family [H]

Homologues:

Organism=Escherichia coli, GI87081983, Length=388, Percent_Identity=27.5773195876289, Blast_Score=106, Evalue=3e-24,
Organism=Escherichia coli, GI1789042, Length=394, Percent_Identity=25.3807106598985, Blast_Score=103, Evalue=3e-23,
Organism=Escherichia coli, GI1788710, Length=312, Percent_Identity=24.6794871794872, Blast_Score=87, Evalue=2e-18,
Organism=Escherichia coli, GI1788392, Length=437, Percent_Identity=27.4599542334096, Blast_Score=76, Evalue=6e-15,
Organism=Escherichia coli, GI1790195, Length=374, Percent_Identity=23.7967914438503, Blast_Score=69, Evalue=9e-13,
Organism=Escherichia coli, GI1790146, Length=162, Percent_Identity=27.1604938271605, Blast_Score=64, Evalue=2e-11,
Organism=Saccharomyces cerevisiae, GI6324954, Length=394, Percent_Identity=22.8426395939086, Blast_Score=86, Evalue=2e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004638
- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196
- InterPro:   IPR001411 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 52560; Mature: 52560

Theoretical pI: Translated: 6.34; Mature: 6.34

Prosite motif: PS50850 MFS ; PS00216 SUGAR_TRANSPORT_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
5.9 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
5.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLHSTSSRNQVALSAVCLSALMFGLEISSVPTILPTLEQLFSADFRQLQWVMNAYTTAMT
CCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ACLMAMGALADRFGRKRVFLAGQVVFGVASLACGLADSPSMLIGARALQGAAAAALLTCQ
HHHHHHHHHHHHHCCCEEEEHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHH
IAVLSQQFRDGRERALAFGWWGVTFGVGLGFGPLIGGLTAVVLGWEWVFLVHVILAAVAV
HHHHHHHHHCCHHHEEEEEEHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ILIQVGVEESTDPHALRLDVAGMVTLSLAVFCLVYLITRGRMPGPGDISGLALAALGVAC
HHHHHCCCCCCCCCEEEEEHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHH
FSAFVVVETRVARPMFDFTAFRTTNFSGALLASAGMNFSFWPFIIYLPVYFQGVLGLDSL
HHHHHHHHHHHHCHHHHHHHHCCCCCCCHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCCC
DAGLCLLAYTLPPLLAPPIAERLLLHFGPQVVIPLGLVIIGCGFVLMRAAALGGDASWVD
HHHHHHHHHHHHHHCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHH
MLPGCILAGSGLGLANTTATNTASAALPPERAGMASGMNMSASMIALAINIALMGFILLQ
HCCCHHEECCCCCCCCCCCCCCCCCCCCCHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHC
GTQAGLERLLPAATGDELSLLAESVAAGGEAVAAGAGLPIALVRQSLVHGFDWVMLYGAG
CCHHHHHHHCCCCCCCHHHHHHHHHHCCCCCEECCCCCHHHHHHHHHHHCCCEEEEECCC
GVWLFAAICKLVFGSAKRSGPAPCDR
HHHHHHHHHHHHHCCCCCCCCCCCCC
>Mature Secondary Structure
MLHSTSSRNQVALSAVCLSALMFGLEISSVPTILPTLEQLFSADFRQLQWVMNAYTTAMT
CCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ACLMAMGALADRFGRKRVFLAGQVVFGVASLACGLADSPSMLIGARALQGAAAAALLTCQ
HHHHHHHHHHHHHCCCEEEEHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHH
IAVLSQQFRDGRERALAFGWWGVTFGVGLGFGPLIGGLTAVVLGWEWVFLVHVILAAVAV
HHHHHHHHHCCHHHEEEEEEHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ILIQVGVEESTDPHALRLDVAGMVTLSLAVFCLVYLITRGRMPGPGDISGLALAALGVAC
HHHHHCCCCCCCCCEEEEEHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHH
FSAFVVVETRVARPMFDFTAFRTTNFSGALLASAGMNFSFWPFIIYLPVYFQGVLGLDSL
HHHHHHHHHHHHCHHHHHHHHCCCCCCCHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCCC
DAGLCLLAYTLPPLLAPPIAERLLLHFGPQVVIPLGLVIIGCGFVLMRAAALGGDASWVD
HHHHHHHHHHHHHHCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHH
MLPGCILAGSGLGLANTTATNTASAALPPERAGMASGMNMSASMIALAINIALMGFILLQ
HCCCHHEECCCCCCCCCCCCCCCCCCCCCHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHC
GTQAGLERLLPAATGDELSLLAESVAAGGEAVAAGAGLPIALVRQSLVHGFDWVMLYGAG
CCHHHHHHHCCCCCCCHHHHHHHHHHCCCCCEECCCCCHHHHHHHHHHHCCCEEEEECCC
GVWLFAAICKLVFGSAKRSGPAPCDR
HHHHHHHHHHHHHCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9634230; 12218036 [H]