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The map label for this gene is ppdK [H]

Identifier: 83594288

GI number: 83594288

Start: 3405623

End: 3408307

Strand: Direct

Name: ppdK [H]

Synonym: Rru_A2956

Alternate gene names: 83594288

Gene position: 3405623-3408307 (Clockwise)

Preceding gene: 83594287

Following gene: 83594290

Centisome position: 78.24

GC content: 66.44

Gene sequence:

>2685_bases
ATGTCCAAGTGGGTTTACAGCTTCGGCAACGGCAAGGCCGAGGGCCGCGCCGATATGAAGAACCTCCTGGGCGGCAAGGG
TGCCAACCTGGCGGAAATGAGCAATCTCGGCATCGCCGTTCCCGCCGGGTTCACCATCACCACCGAGGTCTGCACGGCCT
TTTACGACAACGACCACGCCTATCCGGCCGGATTGACCGATCAGGTGGTCACCGCCCTGGCGCGGGTCGAGGAAATCATG
GGCGCGCGCTTTGGCGACGCCAACAACCCGCTGCTCGTCTCGGTGCGCTCGGGCGCCCGCGCCTCGATGCCGGGCATGAT
GGACACGGTGCTCAACCTCGGGCTTAACGACCAGACGGTTCAGGGGTTGATCAAGCAATCGGGCGACGCCCGCTTCGCCT
ATGACAGCTACCGCCGCTTCATCCAGATGTTCTCGGACGTGGTGCTCGGCGTCGAGCATTACCACTTCGAAGAAATCCTG
GAAAACCACAAGGAAGACAAAGGCCTCAGCCTTGATACCGAGCTGGGCGCCGACGACTGGAAGCTGCTGGTCGAGCGCTT
CAAGGCCAAGGTCCAGCAGGAATTGGGCAAGCCCTTCCCCGAAAACCCCGAAGAGCAGCTGTGGGCGGCCGTCGGCGCGG
TCTTCGGCTCGTGGATGAACCAGCGCGCCATCACCTATCGCCGCCTCAACGACATTCCCCAGGAATGGGGCACGGCGGTG
ACCGTGCAGTCGATGGTCTTTGGCAATATGGGCGATGATTGCGCGACGGGCGTCTGCTTCAGCCGCGATCCGTCGACCGG
CGAGAACACCTTCTACGGCGAATATCTGGTCAACGCCCAGGGCGAGGACGTGGTGGCGGGCATCCGCACCCCCCAGCAGA
TCTCGATCGCCGGCAAGGAAAAGAACGCCTCGGCCCTGCCCGCCATGGAAGAGGTGATGCCCGGCCTTTACAAGGAGTTG
GTCGCCACCCGCGACGTGCTCGAGGCCCATTACCGCGACATGCAGGACATGGAGTTCACCATCCAGGGCGGCAAGCTGTG
GATGCTCCAAACCCGCACCGGCAAGCGCACCGCCGGCGCCGCCCTGCGCATCGCCGTTGAAATGGCCCGCGAAGGCATCA
TCACCAGCGACGAGGCGATTTTGCGCATCGATCCCGAGGCGCTGGACCAGCTTTTGCACCCCACCCTTGATCCCAAGGCC
GCGAAGATCGTGCTGACCAAGGGCCTGCCCGCCTCGCCGGGCGCCGCCTCGGGCAAGGTGGTGTTCTCGGCCGAGGTCGC
CGAAGACTGGGTGCAGCGCGGCGAGAAGGTTATCCTCGTGCGCATCGAGACCAGCCCCGAGGATATCGGCGGCATGCATG
TCTCGCAGGGCATCTTGACGACGCGCGGCGGCATGACCAGCCACGCCGCCGTGGTGGCGCGCGGCATGGGCACGCCCTGC
GTGGCCGGCGCCGGCGAAATCCGCGTCGATTATAAGGCCGGGCTGTTCACCGTTGGCGGCAAGACCGTGCGCGAGGGCGA
CATCATCACCCTTGATGGCGCCGCCGGCACGGTGATGCTGGGCGCGGTGCCGACCATCCAGCCCGAACTGACCGGCGATT
TCGGCGTGCTGATGGACTGGGCCGACGCCAAGCGCACGATGAAGGTGCGGGCCAACGCCGAGACCCCGGTCGACGCCGAG
GCCGCCAAGCGCTTCGGCTGCGAAGGCATCGGGCTCTGTCGCACCGAGCATATGTTCTTCGATCCCCGGCGCATCATCAA
TATCCGCCGCATGATCCTGGCCTCCGACGAGGCCGGCCGTCGCGCCGCCCTGGCCGAGCTTCTGCCCTACCAGCGCGGCG
ATTTCGTCGAGCTGTTCCGCATCATGGCCGGGCTGCCCGTCACCATCCGCCTGCTCGACCCGCCGCTGCACGAGTTCCTG
CCCCATACGGATGGCGAGATCGCCGAGGTCGCCGCCATCGCCGGCGTTGACGAATCCCTGGTTCGCCACCGCGCCAGCGT
GCTGTCGGAAAGCAACCCGATGCTCGGCCATCGCGGCTGCCGCCTGGGGGTGACCTATCCCGAGATCTACGAGATGCAGG
CCCGGGCCATTTTCGAAGCGGCGCTCGAGATCGCCCAGGAGGTCGGCGAGGCGCCGGTGCCCGAGATCATGATCCCGCTG
GTCGCCACCAAGCGCGAGCTTGACCTGATGAAGGCGGTGGTCGACGCCGCCGCCAAGGCGGTCTTCGCCGAAAAGGGCGG
TGAGGTCACCTATAGCGTCGGCACGATGATCGAACTGCCGCGCGCCGCCCTGCAGGCCGGCAGCATCGCCCAGACCGCCC
AGTTCTTCAGCTTCGGCACCAACGACCTGACCCAGACCACGCTTGGCCTGTCGCGCGATGACAGCGCGTCGTTCCTGGAA
ACCTACCGGGTCAAGGGCATCTACGAGCGCGATCCCTTCGCCAGCCTGGATGTCGAGGGCGTGGGCGAATTGGTCAAGAT
CGCCGCCGAACGCGGCCGGGCGACCCGCCCCGGCCTGAAGCTGGGCATCTGCGGCGAACATGGCGGCGATCCGCAGTCCA
TCGCCTTCTGCCAGTCGGTCGGCCTGGATTACGTCTCGTGCTCGCCCTACCGGGTGCCGATCGCCCGGCTGGCCGCCGCC
CAAGCCGCCCTGCGCAGCGTCGTCAAGCAACCCGCGATGGTGTAA

Upstream 100 bases:

>100_bases
CCGATACGCCCGCTCTTCGCAACAATCGCCTGCGCCTGCTCGCGCGCATCGGCACCGCCATGGCCACCCTGGCGGACTTC
TCAAAAATCGAGGGATGATC

Downstream 100 bases:

>100_bases
GCCAGACCTTTCGCCACCGACCGTGATCGCCGAATGGCTCACCAGCGTCCTGACGCCCGCCTCTGTCGAGGCGCGGCGTT
TGGGCGTGGTGGCCGAGGCG

Product: pyruvate phosphate dikinase

Products: NA

Alternate protein names: Pyruvate, orthophosphate dikinase [H]

Number of amino acids: Translated: 894; Mature: 893

Protein sequence:

>894_residues
MSKWVYSFGNGKAEGRADMKNLLGGKGANLAEMSNLGIAVPAGFTITTEVCTAFYDNDHAYPAGLTDQVVTALARVEEIM
GARFGDANNPLLVSVRSGARASMPGMMDTVLNLGLNDQTVQGLIKQSGDARFAYDSYRRFIQMFSDVVLGVEHYHFEEIL
ENHKEDKGLSLDTELGADDWKLLVERFKAKVQQELGKPFPENPEEQLWAAVGAVFGSWMNQRAITYRRLNDIPQEWGTAV
TVQSMVFGNMGDDCATGVCFSRDPSTGENTFYGEYLVNAQGEDVVAGIRTPQQISIAGKEKNASALPAMEEVMPGLYKEL
VATRDVLEAHYRDMQDMEFTIQGGKLWMLQTRTGKRTAGAALRIAVEMAREGIITSDEAILRIDPEALDQLLHPTLDPKA
AKIVLTKGLPASPGAASGKVVFSAEVAEDWVQRGEKVILVRIETSPEDIGGMHVSQGILTTRGGMTSHAAVVARGMGTPC
VAGAGEIRVDYKAGLFTVGGKTVREGDIITLDGAAGTVMLGAVPTIQPELTGDFGVLMDWADAKRTMKVRANAETPVDAE
AAKRFGCEGIGLCRTEHMFFDPRRIINIRRMILASDEAGRRAALAELLPYQRGDFVELFRIMAGLPVTIRLLDPPLHEFL
PHTDGEIAEVAAIAGVDESLVRHRASVLSESNPMLGHRGCRLGVTYPEIYEMQARAIFEAALEIAQEVGEAPVPEIMIPL
VATKRELDLMKAVVDAAAKAVFAEKGGEVTYSVGTMIELPRAALQAGSIAQTAQFFSFGTNDLTQTTLGLSRDDSASFLE
TYRVKGIYERDPFASLDVEGVGELVKIAAERGRATRPGLKLGICGEHGGDPQSIAFCQSVGLDYVSCSPYRVPIARLAAA
QAALRSVVKQPAMV

Sequences:

>Translated_894_residues
MSKWVYSFGNGKAEGRADMKNLLGGKGANLAEMSNLGIAVPAGFTITTEVCTAFYDNDHAYPAGLTDQVVTALARVEEIM
GARFGDANNPLLVSVRSGARASMPGMMDTVLNLGLNDQTVQGLIKQSGDARFAYDSYRRFIQMFSDVVLGVEHYHFEEIL
ENHKEDKGLSLDTELGADDWKLLVERFKAKVQQELGKPFPENPEEQLWAAVGAVFGSWMNQRAITYRRLNDIPQEWGTAV
TVQSMVFGNMGDDCATGVCFSRDPSTGENTFYGEYLVNAQGEDVVAGIRTPQQISIAGKEKNASALPAMEEVMPGLYKEL
VATRDVLEAHYRDMQDMEFTIQGGKLWMLQTRTGKRTAGAALRIAVEMAREGIITSDEAILRIDPEALDQLLHPTLDPKA
AKIVLTKGLPASPGAASGKVVFSAEVAEDWVQRGEKVILVRIETSPEDIGGMHVSQGILTTRGGMTSHAAVVARGMGTPC
VAGAGEIRVDYKAGLFTVGGKTVREGDIITLDGAAGTVMLGAVPTIQPELTGDFGVLMDWADAKRTMKVRANAETPVDAE
AAKRFGCEGIGLCRTEHMFFDPRRIINIRRMILASDEAGRRAALAELLPYQRGDFVELFRIMAGLPVTIRLLDPPLHEFL
PHTDGEIAEVAAIAGVDESLVRHRASVLSESNPMLGHRGCRLGVTYPEIYEMQARAIFEAALEIAQEVGEAPVPEIMIPL
VATKRELDLMKAVVDAAAKAVFAEKGGEVTYSVGTMIELPRAALQAGSIAQTAQFFSFGTNDLTQTTLGLSRDDSASFLE
TYRVKGIYERDPFASLDVEGVGELVKIAAERGRATRPGLKLGICGEHGGDPQSIAFCQSVGLDYVSCSPYRVPIARLAAA
QAALRSVVKQPAMV
>Mature_893_residues
SKWVYSFGNGKAEGRADMKNLLGGKGANLAEMSNLGIAVPAGFTITTEVCTAFYDNDHAYPAGLTDQVVTALARVEEIMG
ARFGDANNPLLVSVRSGARASMPGMMDTVLNLGLNDQTVQGLIKQSGDARFAYDSYRRFIQMFSDVVLGVEHYHFEEILE
NHKEDKGLSLDTELGADDWKLLVERFKAKVQQELGKPFPENPEEQLWAAVGAVFGSWMNQRAITYRRLNDIPQEWGTAVT
VQSMVFGNMGDDCATGVCFSRDPSTGENTFYGEYLVNAQGEDVVAGIRTPQQISIAGKEKNASALPAMEEVMPGLYKELV
ATRDVLEAHYRDMQDMEFTIQGGKLWMLQTRTGKRTAGAALRIAVEMAREGIITSDEAILRIDPEALDQLLHPTLDPKAA
KIVLTKGLPASPGAASGKVVFSAEVAEDWVQRGEKVILVRIETSPEDIGGMHVSQGILTTRGGMTSHAAVVARGMGTPCV
AGAGEIRVDYKAGLFTVGGKTVREGDIITLDGAAGTVMLGAVPTIQPELTGDFGVLMDWADAKRTMKVRANAETPVDAEA
AKRFGCEGIGLCRTEHMFFDPRRIINIRRMILASDEAGRRAALAELLPYQRGDFVELFRIMAGLPVTIRLLDPPLHEFLP
HTDGEIAEVAAIAGVDESLVRHRASVLSESNPMLGHRGCRLGVTYPEIYEMQARAIFEAALEIAQEVGEAPVPEIMIPLV
ATKRELDLMKAVVDAAAKAVFAEKGGEVTYSVGTMIELPRAALQAGSIAQTAQFFSFGTNDLTQTTLGLSRDDSASFLET
YRVKGIYERDPFASLDVEGVGELVKIAAERGRATRPGLKLGICGEHGGDPQSIAFCQSVGLDYVSCSPYRVPIARLAAAQ
AALRSVVKQPAMV

Specific function: Catalyzes the reversible phosphorylation of pyruvate and phosphate [H]

COG id: COG0574

COG function: function code G; Phosphoenolpyruvate synthase/pyruvate phosphate dikinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PEP-utilizing enzyme family [H]

Homologues:

Organism=Escherichia coli, GI1788756, Length=457, Percent_Identity=24.945295404814, Blast_Score=112, Evalue=1e-25,
Organism=Escherichia coli, GI1787994, Length=713, Percent_Identity=25.2454417952314, Blast_Score=103, Evalue=3e-23,
Organism=Escherichia coli, GI1789193, Length=450, Percent_Identity=26, Blast_Score=102, Evalue=1e-22,
Organism=Escherichia coli, GI1788726, Length=586, Percent_Identity=23.037542662116, Blast_Score=87, Evalue=3e-18,

Paralogues:

None

Copy number: 360 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2659 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 4,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR008279
- InterPro:   IPR018274
- InterPro:   IPR023151
- InterPro:   IPR000121
- InterPro:   IPR002192
- InterPro:   IPR010121
- InterPro:   IPR015813 [H]

Pfam domain/function: PF00391 PEP-utilizers; PF02896 PEP-utilizers_C; PF01326 PPDK_N [H]

EC number: =2.7.9.1 [H]

Molecular weight: Translated: 96645; Mature: 96513

Theoretical pI: Translated: 4.91; Mature: 4.91

Prosite motif: PS00370 PEP_ENZYMES_PHOS_SITE ; PS00742 PEP_ENZYMES_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKWVYSFGNGKAEGRADMKNLLGGKGANLAEMSNLGIAVPAGFTITTEVCTAFYDNDHA
CCCEEEECCCCCCCCHHHHHHHHCCCCCCHHHHCCCCEEECCCCEEHHHHHHHHHCCCCC
YPAGLTDQVVTALARVEEIMGARFGDANNPLLVSVRSGARASMPGMMDTVLNLGLNDQTV
CCCCCHHHHHHHHHHHHHHHCCCCCCCCCCEEEEECCCCCCCCCHHHHHHHHCCCCHHHH
QGLIKQSGDARFAYDSYRRFIQMFSDVVLGVEHYHFEEILENHKEDKGLSLDTELGADDW
HHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHH
KLLVERFKAKVQQELGKPFPENPEEQLWAAVGAVFGSWMNQRAITYRRLNDIPQEWGTAV
HHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHCCEE
TVQSMVFGNMGDDCATGVCFSRDPSTGENTFYGEYLVNAQGEDVVAGIRTPQQISIAGKE
EEHHHHHCCCCCHHHCCCEECCCCCCCCCCEEEEEEECCCCCCEEEECCCCCEEEEECCC
KNASALPAMEEVMPGLYKELVATRDVLEAHYRDMQDMEFTIQGGKLWMLQTRTGKRTAGA
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCEEEEEEECCCCCCCCH
ALRIAVEMAREGIITSDEAILRIDPEALDQLLHPTLDPKAAKIVLTKGLPASPGAASGKV
HHHHHHHHHHCCCCCCCCEEEEECHHHHHHHHCCCCCCCCEEEEEECCCCCCCCCCCCCE
VFSAEVAEDWVQRGEKVILVRIETSPEDIGGMHVSQGILTTRGGMTSHAAVVARGMGTPC
EEEHHHHHHHHHCCCEEEEEEEECCCCHHCCEEHHCCEEEECCCCCHHHHHHHCCCCCCC
VAGAGEIRVDYKAGLFTVGGKTVREGDIITLDGAAGTVMLGAVPTIQPELTGDFGVLMDW
CCCCCCEEEEECCCEEEECCCEEECCCEEEECCCCCEEEECCCCCCCCCCCCCHHHEEEC
ADAKRTMKVRANAETPVDAEAAKRFGCEGIGLCRTEHMFFDPRRIINIRRMILASDEAGR
CCCCCEEEEEECCCCCCCHHHHHHCCCCCCCEEEECCEECCHHHHHHHHHHHHCCCCCCH
RAALAELLPYQRGDFVELFRIMAGLPVTIRLLDPPLHEFLPHTDGEIAEVAAIAGVDESL
HHHHHHHCCCCCCCHHHHHHHHCCCCEEEEEECCCHHHHCCCCCCCHHHHHHHHCCHHHH
VRHRASVLSESNPMLGHRGCRLGVTYPEIYEMQARAIFEAALEIAQEVGEAPVPEIMIPL
HHHHHHHHCCCCCCCCCCCCEECCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHH
VATKRELDLMKAVVDAAAKAVFAEKGGEVTYSVGTMIELPRAALQAGSIAQTAQFFSFGT
HHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCHHHHHHHHHHCCCHHHHHHHHHCCC
NDLTQTTLGLSRDDSASFLETYRVKGIYERDPFASLDVEGVGELVKIAAERGRATRPGLK
CCHHHHHCCCCCCCCHHHHHHHHHCCEECCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCE
LGICGEHGGDPQSIAFCQSVGLDYVSCSPYRVPIARLAAAQAALRSVVKQPAMV
EEECCCCCCCHHHHHHHHHCCCCEECCCCCCCHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure 
SKWVYSFGNGKAEGRADMKNLLGGKGANLAEMSNLGIAVPAGFTITTEVCTAFYDNDHA
CCEEEECCCCCCCCHHHHHHHHCCCCCCHHHHCCCCEEECCCCEEHHHHHHHHHCCCCC
YPAGLTDQVVTALARVEEIMGARFGDANNPLLVSVRSGARASMPGMMDTVLNLGLNDQTV
CCCCCHHHHHHHHHHHHHHHCCCCCCCCCCEEEEECCCCCCCCCHHHHHHHHCCCCHHHH
QGLIKQSGDARFAYDSYRRFIQMFSDVVLGVEHYHFEEILENHKEDKGLSLDTELGADDW
HHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHH
KLLVERFKAKVQQELGKPFPENPEEQLWAAVGAVFGSWMNQRAITYRRLNDIPQEWGTAV
HHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHCCEE
TVQSMVFGNMGDDCATGVCFSRDPSTGENTFYGEYLVNAQGEDVVAGIRTPQQISIAGKE
EEHHHHHCCCCCHHHCCCEECCCCCCCCCCEEEEEEECCCCCCEEEECCCCCEEEEECCC
KNASALPAMEEVMPGLYKELVATRDVLEAHYRDMQDMEFTIQGGKLWMLQTRTGKRTAGA
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCEEEEEEECCCCCCCCH
ALRIAVEMAREGIITSDEAILRIDPEALDQLLHPTLDPKAAKIVLTKGLPASPGAASGKV
HHHHHHHHHHCCCCCCCCEEEEECHHHHHHHHCCCCCCCCEEEEEECCCCCCCCCCCCCE
VFSAEVAEDWVQRGEKVILVRIETSPEDIGGMHVSQGILTTRGGMTSHAAVVARGMGTPC
EEEHHHHHHHHHCCCEEEEEEEECCCCHHCCEEHHCCEEEECCCCCHHHHHHHCCCCCCC
VAGAGEIRVDYKAGLFTVGGKTVREGDIITLDGAAGTVMLGAVPTIQPELTGDFGVLMDW
CCCCCCEEEEECCCEEEECCCEEECCCEEEECCCCCEEEECCCCCCCCCCCCCHHHEEEC
ADAKRTMKVRANAETPVDAEAAKRFGCEGIGLCRTEHMFFDPRRIINIRRMILASDEAGR
CCCCCEEEEEECCCCCCCHHHHHHCCCCCCCEEEECCEECCHHHHHHHHHHHHCCCCCCH
RAALAELLPYQRGDFVELFRIMAGLPVTIRLLDPPLHEFLPHTDGEIAEVAAIAGVDESL
HHHHHHHCCCCCCCHHHHHHHHCCCCEEEEEECCCHHHHCCCCCCCHHHHHHHHCCHHHH
VRHRASVLSESNPMLGHRGCRLGVTYPEIYEMQARAIFEAALEIAQEVGEAPVPEIMIPL
HHHHHHHHCCCCCCCCCCCCEECCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHH
VATKRELDLMKAVVDAAAKAVFAEKGGEVTYSVGTMIELPRAALQAGSIAQTAQFFSFGT
HHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCHHHHHHHHHHCCCHHHHHHHHHCCC
NDLTQTTLGLSRDDSASFLETYRVKGIYERDPFASLDVEGVGELVKIAAERGRATRPGLK
CCHHHHHCCCCCCCCHHHHHHHHHCCEECCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCE
LGICGEHGGDPQSIAFCQSVGLDYVSCSPYRVPIARLAAAQAALRSVVKQPAMV
EEECCCCCCCHHHHHHHHHCCCCEECCCCCCCHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11481430; 9168612 [H]