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The map label for this gene is gsiB [H]

Identifier: 83593513

GI number: 83593513

Start: 2529029

End: 2530714

Strand: Reverse

Name: gsiB [H]

Synonym: Rru_A2178

Alternate gene names: 83593513

Gene position: 2530714-2529029 (Counterclockwise)

Preceding gene: 83593516

Following gene: 83593512

Centisome position: 58.14

GC content: 63.35

Gene sequence:

>1686_bases
ATGAGATCAGTGAAATCGACGGCGCGAACGGCCTTTCTCGCCCTGTCTTGCGCCGCCCTGGCTGGCGGCCTGTGGGCGGC
CTCCGGACCCGCGGCCCAGGCCGAGGAGGGCAAGCGCGGCGGATCCCTGCGGCTGCTGGCCCGCGCCGGTGGCGGCACGC
TTGATCCCCATATCAATTATACCGCCCAGTATTTCCAAACGAATTTCATTCTTTATGACGGCTTGGTCGGCTTCAAGAAG
GCCAATGGTAAGGACGGCAACGCCATCGTCGCCGATCTGGCCGAGGCCCTGCCGACGGTCAGCGACGACGGCAAGACCTA
TACCTTCACCCTGCGCAAGGGTGTCCGCTTCTCCAATGGTCAGCCGGTGACCACCGGCGATGTCGTCGCCTCGTTCCAGC
GCATCTTCAAGGTGTCCAGCCCGACCGCCGGAACCTTCTACAACGGCATCCTCGGCGCCGATCACTGTCTGGCCGAGCCG
GCGGGGTGCACCCTGGCCGAGGGGGTGAGCGCCGATCCGGCCAGCGGCACGATCGTTATCAAGCTGGCGGCGGCCGATGC
CGAGTTCCTGCAAAAGCTGTCCTTGCCCCATGCCGCCATCCTGCCCGCCGACGCCCCGGCCAAGGATGTCGGCACCGATG
CCATCCCCGGCACCGGGCCCTATAAGATCGAAAGCTACGACCCCAATACCCGCCTGCGTCTGGTGCGCAATCCCTATTTC
AAGGAATGGAGCAAGGACGCCCAGCCCGACGGCTATGTCGACGAGATCAACTATGACTTCGGTCTGACGGAAGAGGCGGC
GATCACCGCCATCCAGAACGGTCAGGCCGACTGGATGTTCGATCCGCCGCCGCCCGATCGCCTGCCCGAGCTCGGCACCA
AATACGCCAAGCAGGTTCACCTCAATCTGCTCGCGGCGATGTGGTATGCGCCGATGAACGTCAATCTGGCACCGTTCAAT
GACGAGCGGGTGCGCAAGGCGGTCAATTACGCCGTCGATCGCGGCGCCCTGACCAAGCTGTTTGGCGGGGCCAATCTGGC
GACGCCGGCCTGCCAGATCCTGCCGCCCGACTTCCCCTCCTATGAGGCCTATTGCCCCTATACCCAGGCGCCCGGCGCCA
AATGGTCCAAGCCCGATCTGGAAAAGGCCAAGGCCCTGGTCAAGGACTCGGGGACGGCGGGGCAGAAGGTGACGGTGATC
GTCGAGGATAACGCGGTGGCGCGCTCCATCGGCGTCTATCTTCAGGGCGTGCTTGCGCAGCTTGGCTATGACGCCTCGGT
CAAGCCGATCTCGCAGAACATCCAGTTCACCTATATCCAGAACACCGCCAACGCGGTGCAGATCAGCGTCTCGCAGTGGT
ATCAGGATTACCCGGCGGCCTCGAACTTCCTGAACGTGCTGTTTAGCTGCGGCTCCTTCCACCCGGGCTCGGATTCCTCG
ATCAATATGTCGGGCTTCTGCGACAAGACCATCGATGCCCAGATCACCGAGGCCCTGGTCACGGCGACGAAGGATCCGGC
GAAGGCGGCGACCCAATGGACGGCCATCGATAAGGCGATCACCGACAAGGCGCCGGTGGCCGTGTTGTTCACCCCCAAGC
AGGTCACCTTCCTGTCGGCGCGGGTGAAGGGCTTCGTCTTCAATAATCAGACGAAATGGCTGATCAGCCAGTCGTGGGTC
GAGTGA

Upstream 100 bases:

>100_bases
TCCGGGCCTTGCAGTGCTGATCCAATGCTGAGCGTCCCTATCGCGATCAGAAGCTTTCCACCACAGTCCGCAACGGCACA
GGTCCAAGGGTGAAATGACA

Downstream 100 bases:

>100_bases
GCCGAGAGGATCGTCGCGTCGTGAGCTTCGCCTTTTCCACCCGCCCGCCGGCGGGCCAACCAGACGACAAGGGGGGGCGG
GCGCCCCCCCTCACTTCCGA

Product: extracellular solute-binding protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 561; Mature: 561

Protein sequence:

>561_residues
MRSVKSTARTAFLALSCAALAGGLWAASGPAAQAEEGKRGGSLRLLARAGGGTLDPHINYTAQYFQTNFILYDGLVGFKK
ANGKDGNAIVADLAEALPTVSDDGKTYTFTLRKGVRFSNGQPVTTGDVVASFQRIFKVSSPTAGTFYNGILGADHCLAEP
AGCTLAEGVSADPASGTIVIKLAAADAEFLQKLSLPHAAILPADAPAKDVGTDAIPGTGPYKIESYDPNTRLRLVRNPYF
KEWSKDAQPDGYVDEINYDFGLTEEAAITAIQNGQADWMFDPPPPDRLPELGTKYAKQVHLNLLAAMWYAPMNVNLAPFN
DERVRKAVNYAVDRGALTKLFGGANLATPACQILPPDFPSYEAYCPYTQAPGAKWSKPDLEKAKALVKDSGTAGQKVTVI
VEDNAVARSIGVYLQGVLAQLGYDASVKPISQNIQFTYIQNTANAVQISVSQWYQDYPAASNFLNVLFSCGSFHPGSDSS
INMSGFCDKTIDAQITEALVTATKDPAKAATQWTAIDKAITDKAPVAVLFTPKQVTFLSARVKGFVFNNQTKWLISQSWV
E

Sequences:

>Translated_561_residues
MRSVKSTARTAFLALSCAALAGGLWAASGPAAQAEEGKRGGSLRLLARAGGGTLDPHINYTAQYFQTNFILYDGLVGFKK
ANGKDGNAIVADLAEALPTVSDDGKTYTFTLRKGVRFSNGQPVTTGDVVASFQRIFKVSSPTAGTFYNGILGADHCLAEP
AGCTLAEGVSADPASGTIVIKLAAADAEFLQKLSLPHAAILPADAPAKDVGTDAIPGTGPYKIESYDPNTRLRLVRNPYF
KEWSKDAQPDGYVDEINYDFGLTEEAAITAIQNGQADWMFDPPPPDRLPELGTKYAKQVHLNLLAAMWYAPMNVNLAPFN
DERVRKAVNYAVDRGALTKLFGGANLATPACQILPPDFPSYEAYCPYTQAPGAKWSKPDLEKAKALVKDSGTAGQKVTVI
VEDNAVARSIGVYLQGVLAQLGYDASVKPISQNIQFTYIQNTANAVQISVSQWYQDYPAASNFLNVLFSCGSFHPGSDSS
INMSGFCDKTIDAQITEALVTATKDPAKAATQWTAIDKAITDKAPVAVLFTPKQVTFLSARVKGFVFNNQTKWLISQSWV
E
>Mature_561_residues
MRSVKSTARTAFLALSCAALAGGLWAASGPAAQAEEGKRGGSLRLLARAGGGTLDPHINYTAQYFQTNFILYDGLVGFKK
ANGKDGNAIVADLAEALPTVSDDGKTYTFTLRKGVRFSNGQPVTTGDVVASFQRIFKVSSPTAGTFYNGILGADHCLAEP
AGCTLAEGVSADPASGTIVIKLAAADAEFLQKLSLPHAAILPADAPAKDVGTDAIPGTGPYKIESYDPNTRLRLVRNPYF
KEWSKDAQPDGYVDEINYDFGLTEEAAITAIQNGQADWMFDPPPPDRLPELGTKYAKQVHLNLLAAMWYAPMNVNLAPFN
DERVRKAVNYAVDRGALTKLFGGANLATPACQILPPDFPSYEAYCPYTQAPGAKWSKPDLEKAKALVKDSGTAGQKVTVI
VEDNAVARSIGVYLQGVLAQLGYDASVKPISQNIQFTYIQNTANAVQISVSQWYQDYPAASNFLNVLFSCGSFHPGSDSS
INMSGFCDKTIDAQITEALVTATKDPAKAATQWTAIDKAITDKAPVAVLFTPKQVTFLSARVKGFVFNNQTKWLISQSWV
E

Specific function: Part of the ABC transporter complex gsiABCD involved in glutathione import [H]

COG id: COG0747

COG function: function code E; ABC-type dipeptide transport system, periplasmic component

Gene ontology:

Cell location: Periplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 5 family [H]

Homologues:

Organism=Escherichia coli, GI1787052, Length=487, Percent_Identity=26.694045174538, Blast_Score=113, Evalue=3e-26,
Organism=Escherichia coli, GI1789966, Length=509, Percent_Identity=28.2907662082515, Blast_Score=113, Evalue=4e-26,
Organism=Escherichia coli, GI1787495, Length=546, Percent_Identity=24.5421245421245, Blast_Score=91, Evalue=2e-19,
Organism=Escherichia coli, GI1787762, Length=509, Percent_Identity=23.7721021611002, Blast_Score=87, Evalue=3e-18,
Organism=Escherichia coli, GI1787551, Length=482, Percent_Identity=23.8589211618257, Blast_Score=73, Evalue=5e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000914 [H]

Pfam domain/function: PF00496 SBP_bac_5 [H]

EC number: NA

Molecular weight: Translated: 60099; Mature: 60099

Theoretical pI: Translated: 6.38; Mature: 6.38

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRSVKSTARTAFLALSCAALAGGLWAASGPAAQAEEGKRGGSLRLLARAGGGTLDPHINY
CCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCEEEEEECCCCCCCCCCCC
TAQYFQTNFILYDGLVGFKKANGKDGNAIVADLAEALPTVSDDGKTYTFTLRKGVRFSNG
EEEEEEEEEEEEECCEEEECCCCCCCCHHHHHHHHHCCCCCCCCCEEEEEEECCCEECCC
QPVTTGDVVASFQRIFKVSSPTAGTFYNGILGADHCLAEPAGCTLAEGVSADPASGTIVI
CCCCHHHHHHHHHHHHHCCCCCCCHHHHCCCCCHHHHCCCCCCCHHCCCCCCCCCCEEEE
KLAAADAEFLQKLSLPHAAILPADAPAKDVGTDAIPGTGPYKIESYDPNTRLRLVRNPYF
EEECCCHHHHHHHCCCCEEEECCCCCHHHCCCCCCCCCCCEEEECCCCCCEEEEECCCCH
KEWSKDAQPDGYVDEINYDFGLTEEAAITAIQNGQADWMFDPPPPDRLPELGTKYAKQVH
HHHCCCCCCCCCEEEECCCCCCCCHHHEEEEECCCCCEEECCCCCCCCHHHHHHHHHHHH
LNLLAAMWYAPMNVNLAPFNDERVRKAVNYAVDRGALTKLFGGANLATPACQILPPDFPS
HHHHHHHHHCCCCCEECCCCHHHHHHHHHHHHCCCHHHHHHCCCCCCCCCCEECCCCCCC
YEAYCPYTQAPGAKWSKPDLEKAKALVKDSGTAGQKVTVIVEDNAVARSIGVYLQGVLAQ
CCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCCEEEEEEECCHHHHHHHHHHHHHHHH
LGYDASVKPISQNIQFTYIQNTANAVQISVSQWYQDYPAASNFLNVLFSCGSFHPGSDSS
HCCCCCCCCCCCCEEEEEEECCCCEEEEEHHHHHHHCCCHHHHHHHHHHCCCCCCCCCCC
INMSGFCDKTIDAQITEALVTATKDPAKAATQWTAIDKAITDKAPVAVLFTPKQVTFLSA
EEECCCCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCEEEEECCCCEEEEEE
RVKGFVFNNQTKWLISQSWVE
EEEEEEECCCCEEEEECCCCC
>Mature Secondary Structure
MRSVKSTARTAFLALSCAALAGGLWAASGPAAQAEEGKRGGSLRLLARAGGGTLDPHINY
CCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCEEEEEECCCCCCCCCCCC
TAQYFQTNFILYDGLVGFKKANGKDGNAIVADLAEALPTVSDDGKTYTFTLRKGVRFSNG
EEEEEEEEEEEEECCEEEECCCCCCCCHHHHHHHHHCCCCCCCCCEEEEEEECCCEECCC
QPVTTGDVVASFQRIFKVSSPTAGTFYNGILGADHCLAEPAGCTLAEGVSADPASGTIVI
CCCCHHHHHHHHHHHHHCCCCCCCHHHHCCCCCHHHHCCCCCCCHHCCCCCCCCCCEEEE
KLAAADAEFLQKLSLPHAAILPADAPAKDVGTDAIPGTGPYKIESYDPNTRLRLVRNPYF
EEECCCHHHHHHHCCCCEEEECCCCCHHHCCCCCCCCCCCEEEECCCCCCEEEEECCCCH
KEWSKDAQPDGYVDEINYDFGLTEEAAITAIQNGQADWMFDPPPPDRLPELGTKYAKQVH
HHHCCCCCCCCCEEEECCCCCCCCHHHEEEEECCCCCEEECCCCCCCCHHHHHHHHHHHH
LNLLAAMWYAPMNVNLAPFNDERVRKAVNYAVDRGALTKLFGGANLATPACQILPPDFPS
HHHHHHHHHCCCCCEECCCCHHHHHHHHHHHHCCCHHHHHHCCCCCCCCCCEECCCCCCC
YEAYCPYTQAPGAKWSKPDLEKAKALVKDSGTAGQKVTVIVEDNAVARSIGVYLQGVLAQ
CCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCCEEEEEEECCHHHHHHHHHHHHHHHH
LGYDASVKPISQNIQFTYIQNTANAVQISVSQWYQDYPAASNFLNVLFSCGSFHPGSDSS
HCCCCCCCCCCCCEEEEEEECCCCEEEEEHHHHHHHCCCHHHHHHHHHHCCCCCCCCCCC
INMSGFCDKTIDAQITEALVTATKDPAKAATQWTAIDKAITDKAPVAVLFTPKQVTFLSA
EEECCCCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCEEEEECCCCEEEEEE
RVKGFVFNNQTKWLISQSWVE
EEEEEEECCCCEEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA