Click here to switch to the map view.

The map label for this gene is pntB

Identifier: 83593516

GI number: 83593516

Start: 2532856

End: 2534250

Strand: Reverse

Name: pntB

Synonym: Rru_A2181

Alternate gene names: 83593516

Gene position: 2534250-2532856 (Counterclockwise)

Preceding gene: 83593517

Following gene: 83593513

Centisome position: 58.22

GC content: 64.01

Gene sequence:

>1395_bases
ATGACGCATAGTCTTACCATGGCGGCCTATATCGTCGCCGGCGTGCTCTTCATTCTCGCCCTGCGCGGCCTGTCCAATCC
GGAATCGGCGCGCAACGGCAACCGCATGGGCATGGTCGGCATGGCGATCGCCATCCTGACCACGCTGCTGTCGCCTTCGG
TTCAGGCCTATGCCTGGATCGTCCTGGCGATCGCCATCGGCGGCGCCATCGGCACGGTGATCGCCAAAAAGGTGCTGATG
ACCGCCCTGCCGCAGCTTGTCGCCGCCTTCCACTCGCTGGTCGGCATGGCCGCCGTGCTGGTGGCCACCGGCGCCCTGCT
CAATCCCGAAGCCTATGGCATCGGTTCGGCCGGGGCGATCCACGCCGGATCGCTGGTTGAAATGTCGCTCGGCCTGGCCG
TCGGCGCCATCACCTTCTCGGGATCGGTGATCGCCTTTGGCAAGCTTCAGGGGCTGATCGCCGGCAAGCCGGTGACCTTC
CCGATGCAGCATCCGCTGAACGCCGTGCTTGGCATTCTGCTGGTCGTGCTGCTGGTGGTCTTCGCCGCCACCGAAAGCCA
CACCGCCTATTTCGCCCTGATGATCCTGGCCTTCGCCCTTGGCTTCTTGCTGATCATTCCGATCGGCGGCGCCGACATGC
CGGTCGTCATCTCGATGCTCAACAGCTATTCGGGCTGGGCGGCTGCGGGCATTGGCTTCACCCTGGGCAATCCGCTGCTG
ATCATCGCCGGCGCCCTGGTCGGGTCGTCGGGCGCCATCCTCAGCTACATCATGTGCAAGGGCATGAACCGCTCGATCTT
CAACGTCATCCTGGGCGGCTTCGGCAGCGAGGGCGGCGTAGCGGCGGCCGGTGGCGCGGCCGGCGATCGTTCGGTCAAGG
CCGGCAGCGCCGAAGACGCGGCCTTCATCATGAAGAACGCCTCGAAGGTCATCATCGTGCCCGGCTATGGCATGGCGGTG
GCCCAGGCCCAGCACGCCCTGCGCGAAATGGCCGATGTGCTCAAGAAGGAAGGCGTCGAGGTTTCCTACGCCATCCATCC
GGTGGCCGGCCGTATGCCCGGGCATATGAACGTGCTGCTGGCCGAGGCCAATGTGCCCTATGACGAGGTCTTCGAGCTCG
AAGAGATCAACAGCTCGTTCCAGACCGCCGATGTCGCCTTCGTCATCGGCGCCAACGACGTGACCAACCCGGCGGCCAAG
ACCGATCCGTCGAGCCCGATCTACGGCATGCCGATCCTTGACGTTGAAAAGGCCGGAACCGTGCTGTTCATCAAGCGCTC
GATGGCCTCGGGCTATGCCGGCGTCGAGAACGAACTGTTCTTCCGCAACAACACGATGATGCTGTTTGGCGACGCCAAGA
AGATGACCGAGCAGATCGTCCAGGCGATGAACTGA

Upstream 100 bases:

>100_bases
GCCATCCTGCTTGCGAGCGTGAACATCTTCGGCGGGTTCATCGTGACCCAACGAATGCTGGCCATGTTCAAGAAGAAGCA
GAAGTAAGGGGCACGCGACC

Downstream 100 bases:

>100_bases
CCGCTTGAGAGCGGTCATTGATTACGAAAAAGGGCGGGACCGGCAGCGGTCCCGCCCTTTGCGCGATTAGGCGACGCCTT
TTGCCCGGGATCAGCCGTCG

Product: NAD(P) transhydrogenase, subunit beta

Products: NA

Alternate protein names: Nicotinamide nucleotide transhydrogenase subunit beta; Proton-translocating transhydrogenase NADP(H)-binding component; Pyridine nucleotide transhydrogenase subunit beta; dIII

Number of amino acids: Translated: 464; Mature: 463

Protein sequence:

>464_residues
MTHSLTMAAYIVAGVLFILALRGLSNPESARNGNRMGMVGMAIAILTTLLSPSVQAYAWIVLAIAIGGAIGTVIAKKVLM
TALPQLVAAFHSLVGMAAVLVATGALLNPEAYGIGSAGAIHAGSLVEMSLGLAVGAITFSGSVIAFGKLQGLIAGKPVTF
PMQHPLNAVLGILLVVLLVVFAATESHTAYFALMILAFALGFLLIIPIGGADMPVVISMLNSYSGWAAAGIGFTLGNPLL
IIAGALVGSSGAILSYIMCKGMNRSIFNVILGGFGSEGGVAAAGGAAGDRSVKAGSAEDAAFIMKNASKVIIVPGYGMAV
AQAQHALREMADVLKKEGVEVSYAIHPVAGRMPGHMNVLLAEANVPYDEVFELEEINSSFQTADVAFVIGANDVTNPAAK
TDPSSPIYGMPILDVEKAGTVLFIKRSMASGYAGVENELFFRNNTMMLFGDAKKMTEQIVQAMN

Sequences:

>Translated_464_residues
MTHSLTMAAYIVAGVLFILALRGLSNPESARNGNRMGMVGMAIAILTTLLSPSVQAYAWIVLAIAIGGAIGTVIAKKVLM
TALPQLVAAFHSLVGMAAVLVATGALLNPEAYGIGSAGAIHAGSLVEMSLGLAVGAITFSGSVIAFGKLQGLIAGKPVTF
PMQHPLNAVLGILLVVLLVVFAATESHTAYFALMILAFALGFLLIIPIGGADMPVVISMLNSYSGWAAAGIGFTLGNPLL
IIAGALVGSSGAILSYIMCKGMNRSIFNVILGGFGSEGGVAAAGGAAGDRSVKAGSAEDAAFIMKNASKVIIVPGYGMAV
AQAQHALREMADVLKKEGVEVSYAIHPVAGRMPGHMNVLLAEANVPYDEVFELEEINSSFQTADVAFVIGANDVTNPAAK
TDPSSPIYGMPILDVEKAGTVLFIKRSMASGYAGVENELFFRNNTMMLFGDAKKMTEQIVQAMN
>Mature_463_residues
THSLTMAAYIVAGVLFILALRGLSNPESARNGNRMGMVGMAIAILTTLLSPSVQAYAWIVLAIAIGGAIGTVIAKKVLMT
ALPQLVAAFHSLVGMAAVLVATGALLNPEAYGIGSAGAIHAGSLVEMSLGLAVGAITFSGSVIAFGKLQGLIAGKPVTFP
MQHPLNAVLGILLVVLLVVFAATESHTAYFALMILAFALGFLLIIPIGGADMPVVISMLNSYSGWAAAGIGFTLGNPLLI
IAGALVGSSGAILSYIMCKGMNRSIFNVILGGFGSEGGVAAAGGAAGDRSVKAGSAEDAAFIMKNASKVIIVPGYGMAVA
QAQHALREMADVLKKEGVEVSYAIHPVAGRMPGHMNVLLAEANVPYDEVFELEEINSSFQTADVAFVIGANDVTNPAAKT
DPSSPIYGMPILDVEKAGTVLFIKRSMASGYAGVENELFFRNNTMMLFGDAKKMTEQIVQAMN

Specific function: The transhydrogenation between NADH and NADP is coupled to respiration and ATP hydrolysis and functions as a proton pump across the membrane

COG id: COG1282

COG function: function code C; NAD/NADP transhydrogenase beta subunit

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PNT beta subunit family

Homologues:

Organism=Homo sapiens, GI122939155, Length=458, Percent_Identity=46.7248908296943, Blast_Score=370, Evalue=1e-102,
Organism=Homo sapiens, GI122939153, Length=458, Percent_Identity=46.7248908296943, Blast_Score=370, Evalue=1e-102,
Organism=Escherichia coli, GI1787886, Length=467, Percent_Identity=53.7473233404711, Blast_Score=477, Evalue=1e-136,
Organism=Caenorhabditis elegans, GI17550456, Length=460, Percent_Identity=45.6521739130435, Blast_Score=361, Evalue=1e-100,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PNTB_RHORT (Q2RSB4)

Other databases:

- EMBL:   U05294
- EMBL:   CP000230
- RefSeq:   YP_427268.1
- PDB:   1E3T
- PDB:   1HZZ
- PDB:   1NM5
- PDB:   1PNO
- PDB:   1PNQ
- PDB:   1PTJ
- PDB:   1U28
- PDB:   1U2D
- PDB:   1U2G
- PDB:   1XLT
- PDBsum:   1E3T
- PDBsum:   1HZZ
- PDBsum:   1NM5
- PDBsum:   1PNO
- PDBsum:   1PNQ
- PDBsum:   1PTJ
- PDBsum:   1U28
- PDBsum:   1U2D
- PDBsum:   1U2G
- PDBsum:   1XLT
- ProteinModelPortal:   Q2RSB4
- SMR:   Q2RSB4
- STRING:   Q2RSB4
- GeneID:   3835608
- GenomeReviews:   CP000230_GR
- KEGG:   rru:Rru_A2181
- NMPDR:   fig|1085.1.peg.3656
- eggNOG:   COG1282
- HOGENOM:   HBG648813
- OMA:   QSWLPFI
- PhylomeDB:   Q2RSB4
- BioCyc:   RRUB269796:RRU_A2181-MONOMER
- InterPro:   IPR012136
- InterPro:   IPR004003
- PIRSF:   PIRSF000204

Pfam domain/function: PF02233 PNTB

EC number: =1.6.1.2

Molecular weight: Translated: 47809; Mature: 47678

Theoretical pI: Translated: 6.52; Mature: 6.52

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

HASH(0xfba3f24)-; HASH(0x1079236c)-; HASH(0x1030c174)-; HASH(0x10720064)-; HASH(0x10380fe8)-; HASH(0x10788ffc)-;

Cys/Met content:

0.2 %Cys     (Translated Protein)
5.4 %Met     (Translated Protein)
5.6 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
5.2 %Met     (Mature Protein)
5.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTHSLTMAAYIVAGVLFILALRGLSNPESARNGNRMGMVGMAIAILTTLLSPSVQAYAWI
CCCCHHHHHHHHHHHHHHHHHHCCCCCHHHCCCCCCHHHHHHHHHHHHHHCCCHHHHHHH
VLAIAIGGAIGTVIAKKVLMTALPQLVAAFHSLVGMAAVLVATGALLNPEAYGIGSAGAI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCC
HAGSLVEMSLGLAVGAITFSGSVIAFGKLQGLIAGKPVTFPMQHPLNAVLGILLVVLLVV
CCCHHHHHHHHHHEEEEEECCCEEEEECHHHEECCCCCCCCCCCCHHHHHHHHHHHHHHH
FAATESHTAYFALMILAFALGFLLIIPIGGADMPVVISMLNSYSGWAAAGIGFTLGNPLL
HHCCCCCHHHHHHHHHHHHHHHHEEEECCCCCHHHHHHHHHCCCCCCHHCCCCCCCCCHH
IIAGALVGSSGAILSYIMCKGMNRSIFNVILGGFGSEGGVAAAGGAAGDRSVKAGSAEDA
EEEHHHHCCCCHHHHHHHHCCCCHHHHHHHHHCCCCCCCEEECCCCCCCCCCCCCCCCCH
AFIMKNASKVIIVPGYGMAVAQAQHALREMADVLKKEGVEVSYAIHPVAGRMPGHMNVLL
HHHCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCCCCEEEEE
AEANVPYDEVFELEEINSSFQTADVAFVIGANDVTNPAAKTDPSSPIYGMPILDVEKAGT
EECCCCHHHHHHHHHHCCCCCCCEEEEEEECCCCCCCCCCCCCCCCCCCCCCEECCCCCC
VLFIKRSMASGYAGVENELFFRNNTMMLFGDAKKMTEQIVQAMN
EEEEEEHHHCCCCCCCCCEEEECCEEEEEECHHHHHHHHHHHCH
>Mature Secondary Structure 
THSLTMAAYIVAGVLFILALRGLSNPESARNGNRMGMVGMAIAILTTLLSPSVQAYAWI
CCCHHHHHHHHHHHHHHHHHHCCCCCHHHCCCCCCHHHHHHHHHHHHHHCCCHHHHHHH
VLAIAIGGAIGTVIAKKVLMTALPQLVAAFHSLVGMAAVLVATGALLNPEAYGIGSAGAI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCC
HAGSLVEMSLGLAVGAITFSGSVIAFGKLQGLIAGKPVTFPMQHPLNAVLGILLVVLLVV
CCCHHHHHHHHHHEEEEEECCCEEEEECHHHEECCCCCCCCCCCCHHHHHHHHHHHHHHH
FAATESHTAYFALMILAFALGFLLIIPIGGADMPVVISMLNSYSGWAAAGIGFTLGNPLL
HHCCCCCHHHHHHHHHHHHHHHHEEEECCCCCHHHHHHHHHCCCCCCHHCCCCCCCCCHH
IIAGALVGSSGAILSYIMCKGMNRSIFNVILGGFGSEGGVAAAGGAAGDRSVKAGSAEDA
EEEHHHHCCCCHHHHHHHHCCCCHHHHHHHHHCCCCCCCEEECCCCCCCCCCCCCCCCCH
AFIMKNASKVIIVPGYGMAVAQAQHALREMADVLKKEGVEVSYAIHPVAGRMPGHMNVLL
HHHCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCCCCEEEEE
AEANVPYDEVFELEEINSSFQTADVAFVIGANDVTNPAAKTDPSSPIYGMPILDVEKAGT
EECCCCHHHHHHHHHHCCCCCCCEEEEEEECCCCCCCCCCCCCCCCCCCCCCEECCCCCC
VLFIKRSMASGYAGVENELFFRNNTMMLFGDAKKMTEQIVQAMN
EEEEEEHHHCCCCCCCCCEEEECCEEEEEECHHHHHHHHHHHCH

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8075801