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The map label for this gene is glpK

Identifier: 83593344

GI number: 83593344

Start: 2322053

End: 2323540

Strand: Direct

Name: glpK

Synonym: Rru_A2009

Alternate gene names: 83593344

Gene position: 2322053-2323540 (Clockwise)

Preceding gene: 83593343

Following gene: 83593350

Centisome position: 53.35

GC content: 68.48

Gene sequence:

>1488_bases
ATGCCCTTTCTTCTTGCCATCGACCAGGGAACCACCAGCAGCCGGGCCATCGTTTTCGATCACGAGGGCCAGCCGATCGC
CCGGGCCCAAAAGGACCTGGTCCAGTATTTCCCCGGCGATGGCTGGGTTGAACACGACGCGACGGCGATCTGGGAAGACA
GCTTGGCGGTGGCCCGCGAGGCGCTGGACCGGGCCGATGTCGCCGCCCATGCCATCAGCGCCATCGGCCTGACCAATCAA
CGCGAAACCGCCGTGCTGTGGGAGCGCGCCAGCGGCCAGCCGGTTCATAACGCCATCGTCTGGCAAGATCGCCGCACGGC
GGCGCTGTGCCGCGAACTGAAGGCCCAGGGCCATGAGGCGCTGGTGCGGCGCAAGACCGGCCTGCTGATCGATCCCTATT
TCTCGGCCACCAAGATCGGCTGGATGCTTGATCACGATCCGGTGTTGCGCCGGCGGGCCGAAGCCGGCGAGCTGGCGTTC
GGCACGGTCGAATCCTGGCTGCTCTATAAGCTGACCGGCGGCGCCGTCCACGCCAGCGACGCCACGAACGCCGCCCGCAC
CTTGCTGTTCGACATCCGCGCCAACCGTTGGGACGAGGATCTTCTGGCGCTGTTTCGCATCCCCGCCGCCCTGCTGCCCC
GGGTGGTCGATAACGCCGGGCGCTTTGGCGAAACCCTGCCCGGGCTGTTCGGCGCGCCGATCCCGATCACCGGCATGGCC
GGCGACCAGCATGCCGCCATGGTCGGCCAGGGCTGCTTCACCCGGGGCATGATCAAATCGACCTATGGCACCGGCGCCTT
CGCCCTGCTCAACATCGGCCAGACCTTCGTCGAAAGCCGCAATCAATTGCTGACCACCCTGGCCTACCGGCTGAATGGCC
AGAGCACTTATGCCCTGGAAGGCTCGATCTTCGTCGCCGGGGCGGCGGTGCAATGGCTGCGCGACGGGTTGCGGGCGATT
TCCAGCGCCGCCGAAACCCAGGTCCTGGCCGAAGCCGTGGCCGATACGGGCGGCTGCTATATGGTTCCGGCCTTCACCGG
CCTCGGCGCCCCCTATTGGGACCCGGAGGCGCGCGGCGCCATCCTTGGCCTGACGCGCGACACCTCGCTTGAACAGGTCG
CCCGCGCCGCCCTGGAGGCCCAGGGCTATCAAACCCGCGACCTGCTCGATGCCATGGCCGCCGACAGCGGCACCAAGCCG
CTGGCCCTGCGCGTCGATGGCGGCATGGTCGCTAATGATTGGGTCTGTCAGTTCCTGGCCGATATCACCGGCATCGCCGT
TGAACGCCCGCGGGTGATCGAAACCACGGCGCTGGGCGCCGCCGCCCTGGCCGGCCTGGGAGCGGGGGTCTTCGCCAGCC
CGGCCGACCTTGGCGGCCAATGGCACCGCGACCGCCTGTTCACCCCGCATATGCCCGCCAGCCGCCGCGAAAGCCTGTAC
GCCGGCTGGGTCCAGGCGGTGCGCCGGGTGGCCAGCGATCTGCGGTAA

Upstream 100 bases:

>100_bases
CTCGCGCGAAAATGGCGTAAACTGGCGGGCGTTCTTTTGAAAATGGGGGAGGCGGCGGATCATCCCGCCGCTTCCCCGCC
CCGGATCAGGACCGTCCAGC

Downstream 100 bases:

>100_bases
CGGACGATCACATGCCGTCGATCACGGCGCGAACCGCGCTTTCGCCCGCCGGCCGATCGACGGCGAGCCCCTGGGCGCTG
GCTGCCGCTGCCAGGGCGGC

Product: glycerol kinase

Products: NA

Alternate protein names: ATP:glycerol 3-phosphotransferase; Glycerokinase; GK

Number of amino acids: Translated: 495; Mature: 494

Protein sequence:

>495_residues
MPFLLAIDQGTTSSRAIVFDHEGQPIARAQKDLVQYFPGDGWVEHDATAIWEDSLAVAREALDRADVAAHAISAIGLTNQ
RETAVLWERASGQPVHNAIVWQDRRTAALCRELKAQGHEALVRRKTGLLIDPYFSATKIGWMLDHDPVLRRRAEAGELAF
GTVESWLLYKLTGGAVHASDATNAARTLLFDIRANRWDEDLLALFRIPAALLPRVVDNAGRFGETLPGLFGAPIPITGMA
GDQHAAMVGQGCFTRGMIKSTYGTGAFALLNIGQTFVESRNQLLTTLAYRLNGQSTYALEGSIFVAGAAVQWLRDGLRAI
SSAAETQVLAEAVADTGGCYMVPAFTGLGAPYWDPEARGAILGLTRDTSLEQVARAALEAQGYQTRDLLDAMAADSGTKP
LALRVDGGMVANDWVCQFLADITGIAVERPRVIETTALGAAALAGLGAGVFASPADLGGQWHRDRLFTPHMPASRRESLY
AGWVQAVRRVASDLR

Sequences:

>Translated_495_residues
MPFLLAIDQGTTSSRAIVFDHEGQPIARAQKDLVQYFPGDGWVEHDATAIWEDSLAVAREALDRADVAAHAISAIGLTNQ
RETAVLWERASGQPVHNAIVWQDRRTAALCRELKAQGHEALVRRKTGLLIDPYFSATKIGWMLDHDPVLRRRAEAGELAF
GTVESWLLYKLTGGAVHASDATNAARTLLFDIRANRWDEDLLALFRIPAALLPRVVDNAGRFGETLPGLFGAPIPITGMA
GDQHAAMVGQGCFTRGMIKSTYGTGAFALLNIGQTFVESRNQLLTTLAYRLNGQSTYALEGSIFVAGAAVQWLRDGLRAI
SSAAETQVLAEAVADTGGCYMVPAFTGLGAPYWDPEARGAILGLTRDTSLEQVARAALEAQGYQTRDLLDAMAADSGTKP
LALRVDGGMVANDWVCQFLADITGIAVERPRVIETTALGAAALAGLGAGVFASPADLGGQWHRDRLFTPHMPASRRESLY
AGWVQAVRRVASDLR
>Mature_494_residues
PFLLAIDQGTTSSRAIVFDHEGQPIARAQKDLVQYFPGDGWVEHDATAIWEDSLAVAREALDRADVAAHAISAIGLTNQR
ETAVLWERASGQPVHNAIVWQDRRTAALCRELKAQGHEALVRRKTGLLIDPYFSATKIGWMLDHDPVLRRRAEAGELAFG
TVESWLLYKLTGGAVHASDATNAARTLLFDIRANRWDEDLLALFRIPAALLPRVVDNAGRFGETLPGLFGAPIPITGMAG
DQHAAMVGQGCFTRGMIKSTYGTGAFALLNIGQTFVESRNQLLTTLAYRLNGQSTYALEGSIFVAGAAVQWLRDGLRAIS
SAAETQVLAEAVADTGGCYMVPAFTGLGAPYWDPEARGAILGLTRDTSLEQVARAALEAQGYQTRDLLDAMAADSGTKPL
ALRVDGGMVANDWVCQFLADITGIAVERPRVIETTALGAAALAGLGAGVFASPADLGGQWHRDRLFTPHMPASRRESLYA
GWVQAVRRVASDLR

Specific function: Key enzyme in the regulation of glycerol uptake and metabolism

COG id: COG0554

COG function: function code C; Glycerol kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FGGY kinase family

Homologues:

Organism=Homo sapiens, GI41393575, Length=502, Percent_Identity=43.4262948207171, Blast_Score=383, Evalue=1e-106,
Organism=Homo sapiens, GI189409120, Length=502, Percent_Identity=43.2270916334661, Blast_Score=377, Evalue=1e-105,
Organism=Homo sapiens, GI4504007, Length=502, Percent_Identity=43.2270916334661, Blast_Score=377, Evalue=1e-104,
Organism=Homo sapiens, GI42794763, Length=507, Percent_Identity=42.603550295858, Blast_Score=374, Evalue=1e-103,
Organism=Homo sapiens, GI88196792, Length=503, Percent_Identity=31.013916500994, Blast_Score=280, Evalue=3e-75,
Organism=Escherichia coli, GI1790361, Length=488, Percent_Identity=51.2295081967213, Blast_Score=516, Evalue=1e-147,
Organism=Escherichia coli, GI1789987, Length=503, Percent_Identity=25.4473161033797, Blast_Score=117, Evalue=2e-27,
Organism=Escherichia coli, GI1787789, Length=508, Percent_Identity=27.1653543307087, Blast_Score=111, Evalue=1e-25,
Organism=Escherichia coli, GI1790005, Length=460, Percent_Identity=24.3478260869565, Blast_Score=94, Evalue=2e-20,
Organism=Escherichia coli, GI1789168, Length=487, Percent_Identity=24.435318275154, Blast_Score=78, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI17535599, Length=500, Percent_Identity=40.6, Blast_Score=354, Evalue=7e-98,
Organism=Caenorhabditis elegans, GI71986140, Length=521, Percent_Identity=25.1439539347409, Blast_Score=172, Evalue=5e-43,
Organism=Caenorhabditis elegans, GI71986147, Length=297, Percent_Identity=27.6094276094276, Blast_Score=113, Evalue=3e-25,
Organism=Saccharomyces cerevisiae, GI6321755, Length=479, Percent_Identity=37.160751565762, Blast_Score=276, Evalue=7e-75,
Organism=Drosophila melanogaster, GI17864214, Length=503, Percent_Identity=39.9602385685885, Blast_Score=362, Evalue=1e-100,
Organism=Drosophila melanogaster, GI24654573, Length=429, Percent_Identity=41.7249417249417, Blast_Score=336, Evalue=2e-92,
Organism=Drosophila melanogaster, GI24655555, Length=498, Percent_Identity=36.7469879518072, Blast_Score=313, Evalue=3e-85,
Organism=Drosophila melanogaster, GI24655560, Length=498, Percent_Identity=36.7469879518072, Blast_Score=313, Evalue=3e-85,
Organism=Drosophila melanogaster, GI24655570, Length=498, Percent_Identity=36.7469879518072, Blast_Score=313, Evalue=3e-85,
Organism=Drosophila melanogaster, GI24655574, Length=498, Percent_Identity=36.7469879518072, Blast_Score=313, Evalue=3e-85,
Organism=Drosophila melanogaster, GI24655565, Length=498, Percent_Identity=36.7469879518072, Blast_Score=313, Evalue=3e-85,
Organism=Drosophila melanogaster, GI19922058, Length=525, Percent_Identity=33.9047619047619, Blast_Score=286, Evalue=3e-77,
Organism=Drosophila melanogaster, GI24652871, Length=491, Percent_Identity=34.826883910387, Blast_Score=277, Evalue=1e-74,
Organism=Drosophila melanogaster, GI24656367, Length=488, Percent_Identity=29.9180327868852, Blast_Score=214, Evalue=2e-55,
Organism=Drosophila melanogaster, GI21357867, Length=500, Percent_Identity=29, Blast_Score=209, Evalue=3e-54,
Organism=Drosophila melanogaster, GI24656372, Length=324, Percent_Identity=33.3333333333333, Blast_Score=166, Evalue=3e-41,
Organism=Drosophila melanogaster, GI45552909, Length=285, Percent_Identity=29.4736842105263, Blast_Score=120, Evalue=1e-27,

Paralogues:

None

Copy number: 2,500 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): GLPK_RHORT (Q2RST6)

Other databases:

- EMBL:   CP000230
- RefSeq:   YP_427096.1
- ProteinModelPortal:   Q2RST6
- SMR:   Q2RST6
- STRING:   Q2RST6
- GeneID:   3835434
- GenomeReviews:   CP000230_GR
- KEGG:   rru:Rru_A2009
- NMPDR:   fig|1085.1.peg.2584
- eggNOG:   COG0554
- HOGENOM:   HBG511469
- OMA:   GWVEHEP
- PhylomeDB:   Q2RST6
- BioCyc:   RRUB269796:RRU_A2009-MONOMER
- HAMAP:   MF_00186
- InterPro:   IPR000577
- InterPro:   IPR018485
- InterPro:   IPR018483
- InterPro:   IPR018484
- InterPro:   IPR005999
- PANTHER:   PTHR10196
- PANTHER:   PTHR10196:SF9
- TIGRFAMs:   TIGR01311

Pfam domain/function: PF02782 FGGY_C; PF00370 FGGY_N

EC number: =2.7.1.30

Molecular weight: Translated: 53274; Mature: 53143

Theoretical pI: Translated: 6.02; Mature: 6.02

Prosite motif: PS00933 FGGY_KINASES_1; PS00445 FGGY_KINASES_2

Important sites: BINDING 11-11 BINDING 15-15 BINDING 81-81 BINDING 133-133 BINDING 242-242 BINDING 264-264 BINDING 307-307

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPFLLAIDQGTTSSRAIVFDHEGQPIARAQKDLVQYFPGDGWVEHDATAIWEDSLAVARE
CCEEEEECCCCCCCEEEEEECCCCCHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHH
ALDRADVAAHAISAIGLTNQRETAVLWERASGQPVHNAIVWQDRRTAALCRELKAQGHEA
HHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCCCHHEEEECCCHHHHHHHHHHHCCHHH
LVRRKTGLLIDPYFSATKIGWMLDHDPVLRRRAEAGELAFGTVESWLLYKLTGGAVHASD
HHHHHCCCEECCCCCCHHCCEEECCCHHHHHHCCCCCEEHHHHHHEEEEEEECCEEECCC
ATNAARTLLFDIRANRWDEDLLALFRIPAALLPRVVDNAGRFGETLPGLFGAPIPITGMA
CHHHHHHEEEEHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHCCCCCCEECCC
GDQHAAMVGQGCFTRGMIKSTYGTGAFALLNIGQTFVESRNQLLTTLAYRLNGQSTYALE
CCCHHHHHHCCHHHHCHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEC
GSIFVAGAAVQWLRDGLRAISSAAETQVLAEAVADTGGCYMVPAFTGLGAPYWDPEARGA
CCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECHHCCCCCCCCCCCCCE
ILGLTRDTSLEQVARAALEAQGYQTRDLLDAMAADSGTKPLALRVDGGMVANDWVCQFLA
EEEECCCCCHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCEEEEECCCEEHHHHHHHHHH
DITGIAVERPRVIETTALGAAALAGLGAGVFASPADLGGQWHRDRLFTPHMPASRRESLY
HHHHHCCCCCCEEEHHHHHHHHHHHCCCCCCCCCHHCCCCCCCCCCCCCCCCCHHHHHHH
AGWVQAVRRVASDLR
HHHHHHHHHHHHHCC
>Mature Secondary Structure 
PFLLAIDQGTTSSRAIVFDHEGQPIARAQKDLVQYFPGDGWVEHDATAIWEDSLAVARE
CEEEEECCCCCCCEEEEEECCCCCHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHH
ALDRADVAAHAISAIGLTNQRETAVLWERASGQPVHNAIVWQDRRTAALCRELKAQGHEA
HHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCCCHHEEEECCCHHHHHHHHHHHCCHHH
LVRRKTGLLIDPYFSATKIGWMLDHDPVLRRRAEAGELAFGTVESWLLYKLTGGAVHASD
HHHHHCCCEECCCCCCHHCCEEECCCHHHHHHCCCCCEEHHHHHHEEEEEEECCEEECCC
ATNAARTLLFDIRANRWDEDLLALFRIPAALLPRVVDNAGRFGETLPGLFGAPIPITGMA
CHHHHHHEEEEHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHCCCCCCEECCC
GDQHAAMVGQGCFTRGMIKSTYGTGAFALLNIGQTFVESRNQLLTTLAYRLNGQSTYALE
CCCHHHHHHCCHHHHCHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEC
GSIFVAGAAVQWLRDGLRAISSAAETQVLAEAVADTGGCYMVPAFTGLGAPYWDPEARGA
CCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECHHCCCCCCCCCCCCCE
ILGLTRDTSLEQVARAALEAQGYQTRDLLDAMAADSGTKPLALRVDGGMVANDWVCQFLA
EEEECCCCCHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCEEEEECCCEEHHHHHHHHHH
DITGIAVERPRVIETTALGAAALAGLGAGVFASPADLGGQWHRDRLFTPHMPASRRESLY
HHHHHCCCCCCEEEHHHHHHHHHHHCCCCCCCCCHHCCCCCCCCCCCCCCCCCHHHHHHH
AGWVQAVRRVASDLR
HHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA