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The map label for this gene is thiC

Identifier: 83593343

GI number: 83593343

Start: 2320063

End: 2321928

Strand: Direct

Name: thiC

Synonym: Rru_A2008

Alternate gene names: 83593343

Gene position: 2320063-2321928 (Clockwise)

Preceding gene: 83593341

Following gene: 83593344

Centisome position: 53.3

GC content: 66.83

Gene sequence:

>1866_bases
ATGACTGCGCCGTTTTTATCCTCCCTATCCCCCACCAGTCCGCTGGCCAGCGCCACGGCGCCCTTTCCCGGCTCGCGCAA
GGTTTATGCCCGCCCGGCCGACGCCCCCCATTTGCGCGTGCCCTTTCGCGAGATCATCCTGTCCGACCCGGGCGAAGCCC
CGGTGCGGGTGGCCGACCCTTCGGGCCCCTATAGCGATCCCGAGGCGACGATCGATCTGCGCCAGGGATTGGCCCGCCAT
CGGGCGTCTTGGGCCAGCGCCCGGGGCAATTCCACGGTCACGGCCGGCCGCCCCGCCCCGAGTGAAGGCGATTTCGAGGC
CTTTCCCCTGACCTACGCCCCCTTGCGCCGCCGCGATGAGACCCCCTTCACCCAACTGGAATACGCCCGCGCCGGAGTGA
TCACCGACGAGATGATCTATGTGGCGACGCGGGAGAACCTGGGCCGGGACAGCGCCGTGGCCGGGGCCTGCGCGCGGTTG
GCGGGCGGCGAGGCCTTTGGCGCCGCCCTGCCCGCCCATGTCACGCCCGAGTTCGTCCGCGCCGAGATCGCCGCCGGGCG
GGCGATCATCCCGGCCAACATCAACCATCCCGAGCTTGAACCGACGATCATCGGCCGCAACTTCCTGGTCAAGGTCAACG
CCAATATCGGCAATTCGGCCCTGGGATCGTCGATCGAGGACGAGGTGGCAAAGCTGGTCTGGGCCATCCGCTGGGGGGCC
GACACGGTGATGGATCTGTCGACGGGCAAGGCCATCCACGCCACCCGCGAATGGATCTTGCGCAACAGTCCGGTGCCCAT
CGGCACCGTTCCCCTGTATCAGGCTTTGGAAAAGGTGGGCGGCGACGCCACCCGCCTTGACTGGGCGGTGTTCGAAGACA
CCCTGATCGAACAATGCGAACAGGGCGTTGATTATTTCACCATCCATGCCGGGGTGCGGCTGGCCCATATTCCGCTGACC
GCGTCGCGCACCACCGGCATCGTCAGTCGCGGCGGTTCGATCCTGGCCAAATGGTGCTTGTCCCACCACCGCGAGAATTT
CCTTTATGAGCGCTTCGCCGATATCTGCGCCATCCTGCGCCGCTATGACGTGGCCTTTTCGCTAGGCGACGGCCTGCGCC
CGGGATCGGTGGCCGATGCCAATGACGCCGCCCAATTCGCCGAACTCGACACTTTGGGCGCCTTGACGGCGGTGGCCTGG
GAGCACGGCTGTCAGGTGATGGTCGAAGGCCCGGGCCATGTGCCCATGCACAAGATCAAGGCCAATATGGACCGCCAACT
CGCCACCTGCGGCGAGGCGCCGTTCTATACCCTGGGGCCCTTGACCACCGATATCGCGCCCGGCCACGACCACATCACCT
CGGCGATCGGCGCGGCGATGATCGGCTGGTTCGGCACCGCCATGCTGTGCTACGTCACCCCCAAGGAACACCTGGGGCTG
CCCGATCGCGCCGACGTCAAGGCCGGGGTGATCGCCTATAAGCTGGCCGCCCATGCCGCCGATATCGCCAAGGGGCATCC
CGCCGCCCAGCTGCGCGACGACGCCATCAGCAGGGCGCGCTTCGATTTCCGCTGGAGCGACCAGTTCAACCTCGGCCTCG
ATCCCGAAGGCGCGCGGGCCTTCCACGACGAAACCCTGCCCCATGCCGCCCATAAGACGGCGCATTTCTGTTCGATGTGC
GGGCCGAAGTTCTGTTCGATGAAGATCAGCCATGATATCCGCGACGGCGCCCTCGAGGGGGCGGACGCCCTGACCCAGGC
CGGACTGGACCAGATGAGTGCGACCTTCCGCGCCAGCGGCGGCGAGGTCCACCTTGATGCGCAAGCTCTCGACGCCCTCG
CCTGGGAGGGGAAACCCGCGCGATAA

Upstream 100 bases:

>100_bases
GCGAAGGGACGGAACGCGGGCCGGCGCACTCGTTCCCTTCGGCGGCCCCCTCCCCTCCTCCCCGTCAGCAGACCGCCCTT
TTTCTCGACGGAGAAGAGCG

Downstream 100 bases:

>100_bases
GAAGCCGGGGCAAGGGCGTTCGCCCTCGCGCGAAAATGGCGTAAACTGGCGGGCGTTCTTTTGAAAATGGGGGAGGCGGC
GGATCATCCCGCCGCTTCCC

Product: thiamine biosynthesis protein ThiC

Products: @AMIN06.txt*4-amino-2-methyl-5-hydroxymethylpyrimidine!; 4-amino-5-hydroxymethyl-2-methylpyrimidine-phosphate [C]

Alternate protein names: Hydroxymethylpyrimidine phosphate synthase; HMP-P synthase; HMP-phosphate synthase; HMPP synthase; Thiamine biosynthesis protein thiC

Number of amino acids: Translated: 621; Mature: 620

Protein sequence:

>621_residues
MTAPFLSSLSPTSPLASATAPFPGSRKVYARPADAPHLRVPFREIILSDPGEAPVRVADPSGPYSDPEATIDLRQGLARH
RASWASARGNSTVTAGRPAPSEGDFEAFPLTYAPLRRRDETPFTQLEYARAGVITDEMIYVATRENLGRDSAVAGACARL
AGGEAFGAALPAHVTPEFVRAEIAAGRAIIPANINHPELEPTIIGRNFLVKVNANIGNSALGSSIEDEVAKLVWAIRWGA
DTVMDLSTGKAIHATREWILRNSPVPIGTVPLYQALEKVGGDATRLDWAVFEDTLIEQCEQGVDYFTIHAGVRLAHIPLT
ASRTTGIVSRGGSILAKWCLSHHRENFLYERFADICAILRRYDVAFSLGDGLRPGSVADANDAAQFAELDTLGALTAVAW
EHGCQVMVEGPGHVPMHKIKANMDRQLATCGEAPFYTLGPLTTDIAPGHDHITSAIGAAMIGWFGTAMLCYVTPKEHLGL
PDRADVKAGVIAYKLAAHAADIAKGHPAAQLRDDAISRARFDFRWSDQFNLGLDPEGARAFHDETLPHAAHKTAHFCSMC
GPKFCSMKISHDIRDGALEGADALTQAGLDQMSATFRASGGEVHLDAQALDALAWEGKPAR

Sequences:

>Translated_621_residues
MTAPFLSSLSPTSPLASATAPFPGSRKVYARPADAPHLRVPFREIILSDPGEAPVRVADPSGPYSDPEATIDLRQGLARH
RASWASARGNSTVTAGRPAPSEGDFEAFPLTYAPLRRRDETPFTQLEYARAGVITDEMIYVATRENLGRDSAVAGACARL
AGGEAFGAALPAHVTPEFVRAEIAAGRAIIPANINHPELEPTIIGRNFLVKVNANIGNSALGSSIEDEVAKLVWAIRWGA
DTVMDLSTGKAIHATREWILRNSPVPIGTVPLYQALEKVGGDATRLDWAVFEDTLIEQCEQGVDYFTIHAGVRLAHIPLT
ASRTTGIVSRGGSILAKWCLSHHRENFLYERFADICAILRRYDVAFSLGDGLRPGSVADANDAAQFAELDTLGALTAVAW
EHGCQVMVEGPGHVPMHKIKANMDRQLATCGEAPFYTLGPLTTDIAPGHDHITSAIGAAMIGWFGTAMLCYVTPKEHLGL
PDRADVKAGVIAYKLAAHAADIAKGHPAAQLRDDAISRARFDFRWSDQFNLGLDPEGARAFHDETLPHAAHKTAHFCSMC
GPKFCSMKISHDIRDGALEGADALTQAGLDQMSATFRASGGEVHLDAQALDALAWEGKPAR
>Mature_620_residues
TAPFLSSLSPTSPLASATAPFPGSRKVYARPADAPHLRVPFREIILSDPGEAPVRVADPSGPYSDPEATIDLRQGLARHR
ASWASARGNSTVTAGRPAPSEGDFEAFPLTYAPLRRRDETPFTQLEYARAGVITDEMIYVATRENLGRDSAVAGACARLA
GGEAFGAALPAHVTPEFVRAEIAAGRAIIPANINHPELEPTIIGRNFLVKVNANIGNSALGSSIEDEVAKLVWAIRWGAD
TVMDLSTGKAIHATREWILRNSPVPIGTVPLYQALEKVGGDATRLDWAVFEDTLIEQCEQGVDYFTIHAGVRLAHIPLTA
SRTTGIVSRGGSILAKWCLSHHRENFLYERFADICAILRRYDVAFSLGDGLRPGSVADANDAAQFAELDTLGALTAVAWE
HGCQVMVEGPGHVPMHKIKANMDRQLATCGEAPFYTLGPLTTDIAPGHDHITSAIGAAMIGWFGTAMLCYVTPKEHLGLP
DRADVKAGVIAYKLAAHAADIAKGHPAAQLRDDAISRARFDFRWSDQFNLGLDPEGARAFHDETLPHAAHKTAHFCSMCG
PKFCSMKISHDIRDGALEGADALTQAGLDQMSATFRASGGEVHLDAQALDALAWEGKPAR

Specific function: Catalyzes the synthesis of the hydroxymethylpyrimidine phosphate (HMP-P) moiety of thiamine from aminoimidazole ribotide (AIR) in a radical S-adenosyl-L-methionine (SAM)-dependent reaction

COG id: COG0422

COG function: function code H; Thiamine biosynthesis protein ThiC

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thiC family

Homologues:

Organism=Escherichia coli, GI1790427, Length=604, Percent_Identity=63.0794701986755, Blast_Score=771, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): THIC_RHORT (Q2RST7)

Other databases:

- EMBL:   CP000230
- RefSeq:   YP_427095.1
- STRING:   Q2RST7
- GeneID:   3835433
- GenomeReviews:   CP000230_GR
- KEGG:   rru:Rru_A2008
- NMPDR:   fig|1085.1.peg.2583
- eggNOG:   COG0422
- HOGENOM:   HBG285234
- OMA:   TWELFRD
- PhylomeDB:   Q2RST7
- ProtClustDB:   PRK09284
- BioCyc:   RRUB269796:RRU_A2008-MONOMER
- HAMAP:   MF_00089
- InterPro:   IPR002817
- TIGRFAMs:   TIGR00190

Pfam domain/function: PF01964 ThiC

EC number: NA

Molecular weight: Translated: 66603; Mature: 66472

Theoretical pI: Translated: 6.26; Mature: 6.26

Prosite motif: NA

Important sites: BINDING 215-215 BINDING 244-244 BINDING 273-273 BINDING 309-309 BINDING 409-409 BINDING 436-436

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTAPFLSSLSPTSPLASATAPFPGSRKVYARPADAPHLRVPFREIILSDPGEAPVRVADP
CCCCCCCCCCCCCCCCCCCCCCCCCCEEEECCCCCCCEECCHHHHHCCCCCCCCEEEECC
SGPYSDPEATIDLRQGLARHRASWASARGNSTVTAGRPAPSEGDFEAFPLTYAPLRRRDE
CCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCEEECCCCCCCCCCCCCCCCCHHHHHCCCC
TPFTQLEYARAGVITDEMIYVATRENLGRDSAVAGACARLAGGEAFGAALPAHVTPEFVR
CCCHHHHHHHCCCCCCCEEEEEECCCCCCCHHHHHHHHHHCCCCHHHCCCCCCCCHHHHH
AEIAAGRAIIPANINHPELEPTIIGRNFLVKVNANIGNSALGSSIEDEVAKLVWAIRWGA
HHHHCCCEEEECCCCCCCCCEEEECCEEEEEEECCCCCHHHCCCHHHHHHHHHHHHHCCC
DTVMDLSTGKAIHATREWILRNSPVPIGTVPLYQALEKVGGDATRLDWAVFEDTLIEQCE
CCEEECCCCCCHHHHHHHHHCCCCCCCCCCHHHHHHHHHCCCCCEEHHHHHHHHHHHHHH
QGVDYFTIHAGVRLAHIPLTASRTTGIVSRGGSILAKWCLSHHRENFLYERFADICAILR
CCCCEEEEECCCEEEEEEEECCCCCHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RYDVAFSLGDGLRPGSVADANDAAQFAELDTLGALTAVAWEHGCQVMVEGPGHVPMHKIK
HHHHEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCHHHHH
ANMDRQLATCGEAPFYTLGPLTTDIAPGHDHITSAIGAAMIGWFGTAMLCYVTPKEHLGL
HCHHHHHHHCCCCCCEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHEEEEEECCHHHCCC
PDRADVKAGVIAYKLAAHAADIAKGHPAAQLRDDAISRARFDFRWSDQFNLGLDPEGARA
CCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCEEECCCCCCCCCCCCCHH
FHDETLPHAAHKTAHFCSMCGPKFCSMKISHDIRDGALEGADALTQAGLDQMSATFRASG
HHHHCCCHHHHHHHHHHHHCCCHHEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHCCCC
GEVHLDAQALDALAWEGKPAR
CEEEECHHHHHHHHCCCCCCC
>Mature Secondary Structure 
TAPFLSSLSPTSPLASATAPFPGSRKVYARPADAPHLRVPFREIILSDPGEAPVRVADP
CCCCCCCCCCCCCCCCCCCCCCCCCEEEECCCCCCCEECCHHHHHCCCCCCCCEEEECC
SGPYSDPEATIDLRQGLARHRASWASARGNSTVTAGRPAPSEGDFEAFPLTYAPLRRRDE
CCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCEEECCCCCCCCCCCCCCCCCHHHHHCCCC
TPFTQLEYARAGVITDEMIYVATRENLGRDSAVAGACARLAGGEAFGAALPAHVTPEFVR
CCCHHHHHHHCCCCCCCEEEEEECCCCCCCHHHHHHHHHHCCCCHHHCCCCCCCCHHHHH
AEIAAGRAIIPANINHPELEPTIIGRNFLVKVNANIGNSALGSSIEDEVAKLVWAIRWGA
HHHHCCCEEEECCCCCCCCCEEEECCEEEEEEECCCCCHHHCCCHHHHHHHHHHHHHCCC
DTVMDLSTGKAIHATREWILRNSPVPIGTVPLYQALEKVGGDATRLDWAVFEDTLIEQCE
CCEEECCCCCCHHHHHHHHHCCCCCCCCCCHHHHHHHHHCCCCCEEHHHHHHHHHHHHHH
QGVDYFTIHAGVRLAHIPLTASRTTGIVSRGGSILAKWCLSHHRENFLYERFADICAILR
CCCCEEEEECCCEEEEEEEECCCCCHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RYDVAFSLGDGLRPGSVADANDAAQFAELDTLGALTAVAWEHGCQVMVEGPGHVPMHKIK
HHHHEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCHHHHH
ANMDRQLATCGEAPFYTLGPLTTDIAPGHDHITSAIGAAMIGWFGTAMLCYVTPKEHLGL
HCHHHHHHHCCCCCCEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHEEEEEECCHHHCCC
PDRADVKAGVIAYKLAAHAADIAKGHPAAQLRDDAISRARFDFRWSDQFNLGLDPEGARA
CCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCEEECCCCCCCCCCCCCHH
FHDETLPHAAHKTAHFCSMCGPKFCSMKISHDIRDGALEGADALTQAGLDQMSATFRASG
HHHHCCCHHHHHHHHHHHHCCCHHEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHCCCC
GEVHLDAQALDALAWEGKPAR
CEEEECHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ?; ?; 5'-phosphoribosyl-5-aminoimidazole [C]

Specific reaction: Synthesis of @AMIN06.txt*4-amino-2-methyl-5-hydroxymethylpyrimidine! 5'-phosphoribosyl-5-aminoimidazole = 4-amino-5-hydroxymethyl-2-methylpyrimidine-phosphate [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA