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The map label for this gene is fruK [H]

Identifier: 83593306

GI number: 83593306

Start: 2279258

End: 2280241

Strand: Reverse

Name: fruK [H]

Synonym: Rru_A1971

Alternate gene names: 83593306

Gene position: 2280241-2279258 (Counterclockwise)

Preceding gene: 83593307

Following gene: 83593305

Centisome position: 52.39

GC content: 68.7

Gene sequence:

>984_bases
ATGAGCAGCGATCCCTCCGCCTTGCCCTCCGCCTTGATTGTCCGCACCCTGACCCTTAACCCCGCCATCGATCAGACCAT
CACCCTTGAGGCCCTGACCCCGGGAACCGTCCACCGCGCTCGCGCCACCCGCTCGGACGCCGGCGGCAAGGGGATCAACG
TCGCCGCCTGTCTGGCCGATTGGGGCGAGAGCGTCGCCGTCTATGGCGTTCTGGGGCGGGATAATTCCGGCCCCTTCGAC
GCCCTGTTCGCCGCCAAGGGCATGATCGACCGCTTCGTGCGCATCCCCGGCGAGACCCGCACCAACATCAAGCTGGTCAG
CAGCGGCGGCGGGCCGACCACCGATATCAATCTGCCGGGGTTGGAAATCGATCGCCCCTGCCTTGAGCGGGTGGCGACGG
CCTTATTGGCCGATCTGGCGCCGGGCACGCCGGTGGTGCTGTCGGGTAGCCTGCCGCGCGGGCTTGATGACAAGACCTAT
GTCGGACTGGTCGGGGCTTTGCGCGAACGCGGCGCGAAAGTGGTGCTTGATGTCAGCGGCTGGCCCCTGACCCGCGCCCT
GGAAGCTCCGGCCGATCACCTGCCCCATTGCGTCAAGCCCAACCGCCACGAGCTGGAATCCTGGGCCGGCCGCGCCTTGC
CGACGCTGGCCGATGTTCAAGAGGCCGCCCATGGCTTGCGCGGGAAGGGGGTTGCCCGGGTGGTGGTGTCGCTGGGCTCG
GATGGGGCGTTGTTTGTCTCCGACGAGGGGGCCCTGCACGCCAAATTGCCGGCGATGACGGCGCTGAGCACGGTCGGGGC
CGGCGACGCCATGGTCGCCGGTCTGGTCGCGGCGCTGCGCCAGGACCTGGGTCTTGAAGACACCGCCCGGCTGGCGACGG
CCTTCGCCGCGGCCAAGCTTCGCCATGTCGGCGCCCAATTACCCGGACGCGACGTCGTTCTCGCCCTGGCCGGGCAAACC
CATATCGCGCGTCTTCAAGCCTAA

Upstream 100 bases:

>100_bases
GCCTGCGCGATTGCGACGCCACGGCCTTGCGCGCCCTGGCCGAACGCGCCCTGACCTGCGAGACCGCGACCGATGTGCGC
GCCCTGGAGGCCGACTTGTG

Downstream 100 bases:

>100_bases
CGCGTTGCCAACGCGGGCGCAGACCCGCCGGGGAGGAGATATCATGAGCCAGTTTATCGCGGTCGTCGGCGGGGGCGAGC
GGAGCACCCAGGCGCTGCTG

Product: PfkB

Products: NA

Alternate protein names: Fructose 1-phosphate kinase [H]

Number of amino acids: Translated: 327; Mature: 326

Protein sequence:

>327_residues
MSSDPSALPSALIVRTLTLNPAIDQTITLEALTPGTVHRARATRSDAGGKGINVAACLADWGESVAVYGVLGRDNSGPFD
ALFAAKGMIDRFVRIPGETRTNIKLVSSGGGPTTDINLPGLEIDRPCLERVATALLADLAPGTPVVLSGSLPRGLDDKTY
VGLVGALRERGAKVVLDVSGWPLTRALEAPADHLPHCVKPNRHELESWAGRALPTLADVQEAAHGLRGKGVARVVVSLGS
DGALFVSDEGALHAKLPAMTALSTVGAGDAMVAGLVAALRQDLGLEDTARLATAFAAAKLRHVGAQLPGRDVVLALAGQT
HIARLQA

Sequences:

>Translated_327_residues
MSSDPSALPSALIVRTLTLNPAIDQTITLEALTPGTVHRARATRSDAGGKGINVAACLADWGESVAVYGVLGRDNSGPFD
ALFAAKGMIDRFVRIPGETRTNIKLVSSGGGPTTDINLPGLEIDRPCLERVATALLADLAPGTPVVLSGSLPRGLDDKTY
VGLVGALRERGAKVVLDVSGWPLTRALEAPADHLPHCVKPNRHELESWAGRALPTLADVQEAAHGLRGKGVARVVVSLGS
DGALFVSDEGALHAKLPAMTALSTVGAGDAMVAGLVAALRQDLGLEDTARLATAFAAAKLRHVGAQLPGRDVVLALAGQT
HIARLQA
>Mature_326_residues
SSDPSALPSALIVRTLTLNPAIDQTITLEALTPGTVHRARATRSDAGGKGINVAACLADWGESVAVYGVLGRDNSGPFDA
LFAAKGMIDRFVRIPGETRTNIKLVSSGGGPTTDINLPGLEIDRPCLERVATALLADLAPGTPVVLSGSLPRGLDDKTYV
GLVGALRERGAKVVLDVSGWPLTRALEAPADHLPHCVKPNRHELESWAGRALPTLADVQEAAHGLRGKGVARVVVSLGSD
GALFVSDEGALHAKLPAMTALSTVGAGDAMVAGLVAALRQDLGLEDTARLATAFAAAKLRHVGAQLPGRDVVLALAGQTH
IARLQA

Specific function: Unknown

COG id: COG1105

COG function: function code G; Fructose-1-phosphate kinase and related fructose-6-phosphate kinase (PfkB)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the carbohydrate kinase pfkB family [H]

Homologues:

Organism=Escherichia coli, GI1788493, Length=277, Percent_Identity=42.9602888086643, Blast_Score=195, Evalue=3e-51,
Organism=Escherichia coli, GI1788017, Length=299, Percent_Identity=31.1036789297659, Blast_Score=115, Evalue=4e-27,
Organism=Escherichia coli, GI87081971, Length=272, Percent_Identity=29.0441176470588, Blast_Score=70, Evalue=3e-13,
Organism=Escherichia coli, GI1788484, Length=283, Percent_Identity=27.9151943462898, Blast_Score=61, Evalue=8e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022463
- InterPro:   IPR011611
- InterPro:   IPR002173
- InterPro:   IPR017583 [H]

Pfam domain/function: PF00294 PfkB [H]

EC number: =2.7.1.56 [H]

Molecular weight: Translated: 33721; Mature: 33590

Theoretical pI: Translated: 7.25; Mature: 7.25

Prosite motif: PS00583 PFKB_KINASES_1 ; PS00584 PFKB_KINASES_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSDPSALPSALIVRTLTLNPAIDQTITLEALTPGTVHRARATRSDAGGKGINVAACLAD
CCCCCCHHHHHHHHEEEECCCCCCCEEEEEECCCCCHHHHHHCCCCCCCCCCCHHHHHHH
WGESVAVYGVLGRDNSGPFDALFAAKGMIDRFVRIPGETRTNIKLVSSGGGPTTDINLPG
CCCCEEEEEEEECCCCCCHHHHHHHHHHHHHHHCCCCCCCCCEEEEECCCCCCCCCCCCC
LEIDRPCLERVATALLADLAPGTPVVLSGSLPRGLDDKTYVGLVGALRERGAKVVLDVSG
CCCCHHHHHHHHHHHHHHCCCCCCEEEECCCCCCCCCCHHHHHHHHHHHCCCEEEEEECC
WPLTRALEAPADHLPHCVKPNRHELESWAGRALPTLADVQEAAHGLRGKGVARVVVSLGS
CCHHHHHHCCHHHCCCCCCCCHHHHHHHHCCCCCHHHHHHHHHHCCCCCCCEEEEEEECC
DGALFVSDEGALHAKLPAMTALSTVGAGDAMVAGLVAALRQDLGLEDTARLATAFAAAKL
CCEEEEECCCCEEECCHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHH
RHVGAQLPGRDVVLALAGQTHIARLQA
HHHHCCCCCCCEEEEECCCCHHHEECC
>Mature Secondary Structure 
SSDPSALPSALIVRTLTLNPAIDQTITLEALTPGTVHRARATRSDAGGKGINVAACLAD
CCCCCHHHHHHHHEEEECCCCCCCEEEEEECCCCCHHHHHHCCCCCCCCCCCHHHHHHH
WGESVAVYGVLGRDNSGPFDALFAAKGMIDRFVRIPGETRTNIKLVSSGGGPTTDINLPG
CCCCEEEEEEEECCCCCCHHHHHHHHHHHHHHHCCCCCCCCCEEEEECCCCCCCCCCCCC
LEIDRPCLERVATALLADLAPGTPVVLSGSLPRGLDDKTYVGLVGALRERGAKVVLDVSG
CCCCHHHHHHHHHHHHHHCCCCCCEEEECCCCCCCCCCHHHHHHHHHHHCCCEEEEEECC
WPLTRALEAPADHLPHCVKPNRHELESWAGRALPTLADVQEAAHGLRGKGVARVVVSLGS
CCHHHHHHCCHHHCCCCCCCCHHHHHHHHCCCCCHHHHHHHHHHCCCCCCCEEEEEEECC
DGALFVSDEGALHAKLPAMTALSTVGAGDAMVAGLVAALRQDLGLEDTARLATAFAAAKL
CCEEEEECCCCEEECCHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHH
RHVGAQLPGRDVVLALAGQTHIARLQA
HHHHCCCCCCCEEEEECCCCHHHEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1655739; 12024217 [H]