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The map label for this gene is fruB [H]

Identifier: 83593307

GI number: 83593307

Start: 2280241

End: 2282748

Strand: Reverse

Name: fruB [H]

Synonym: Rru_A1972

Alternate gene names: 83593307

Gene position: 2282748-2280241 (Counterclockwise)

Preceding gene: 83593309

Following gene: 83593306

Centisome position: 52.44

GC content: 69.62

Gene sequence:

>2508_bases
ATGGGGTCCGTCGATACGGTAGGGGCCTTGCCCCGCGATCTCGTGCGTTTGGCGGTCAGCCCGCGCGACAAGGCCGATGC
CATCGCCCAGGCGGCGCAGATGCTGGTGGCGGCGGGCTGCGTTGAAGCGGGCTATGCCCAAAGCATGATCCGCCGCGAGG
GGGTGGCCAACACCTTCCTGGGTCACGGCGTCGCCATTCCCCACGGCATGGCCGAAGACCGCGCCCTGGTGCGCGCCAAT
GGCATCGCCGTGCTTCAGGTCCCCGATGGCGTGGAATGGAACCCCGGCCAGTCGGCCCGGCTGGTCGTCGCCATCGCCGC
CCAATCCGATACCCATATCGCCATCTTGCGCCGCCTGACCCGCCTGCTTCAGGACGAGGACCGCCTGGGCGGCCTGTTCG
TGACCAGCGATCCCGGCGACGTGATCACCGCCCTGACCACCGACCCCGAGGTTAACGCCCCGGCTGTTCCGGTTAGCGAT
CTGGCGAAGCGCTTCGAATGGGTGCTCGATTATCCCAACGGCCTGCATGCCCGGCCGGCGGCGCGCTGGGTCGATACGGC
CAAGGGCTATGCGGCGCGCTGTCAGATCCGCCACGGCGACGAGGTGGCCGATGTCAAGAATCTGGTGGCCCTGCTGCGCC
TTGGCCTGACCCAGGGCGAGCGGATTTCGGTTTCGGCCGAGGGGCCCGATGCCGATCAGGCGCTGTCCCGGCTCAAGGCG
ACCATGCTCGATCTTTCCGTTCAGGAACGCGCCGATGCCGCCCTGGCGGCGCGCCGCGCCAAGCAGCCGGTGATCGGCTG
GCACCCGCCGGGCGCGCCGATGGCCTTGGCCGGGATCGCCGCCGCGCCCGGGCTGGCGATCGGCCGCCTTCACGTGCTGG
CCGCCGCCGACCGCAGCGTTCCCGACCACCCGGTGCCGTTGAGCGAGGGCGGCAATATCCTGCACGAGGCGCTGAGCGCC
ACCCGCCTGCAGCTCAAGGCCCTGGCCGATGACACGGCGCGTCGGCTTGGTCCCTCGGAAGCCGGCATCTTCAAGGCCCA
GGGCGAATTGCTCAATGATACGGATCTGATCACCCTGACCTGCCAGTTGATGGTGGAAGGCCATGGCGTGGCTTGGTCGT
GGGATCAGGCGATCGAACGCATGGCCGGCAAGCTGGCGGCTTTGGGCAATGCCCTGCTGGTCGCCCGCGCCGCCGATCTG
CGCGATGTCGGCCGGCGGGTTCTGGGCCAGCTTGATCCCGGCCTAGGCGGCGCGTCGTTGCACGACTTGCCCGACGAGCC
CTGCATTCTGATCGCGGGCGATCTAGCGCCTTCGGAAACCGCCGGCCTTGATCCGTCCCGCGTCGTCGGGCTGGCCACCG
CCCAGGGCGGGCCGACCGCCCATACCGCCATTCTCGCCCGGACCATGGGGCTGCCGGCGATGGTCGCCGCCGGTCCCGGA
CTGCTGGCCCTGGAAAGCGGCACTTTGGCCATCCTTGATGGCTATGGCGGGCGGCTTTACCCCGATCCCTCGCCCGAGGC
GCTGTCTTCGGCCCGCGCCTTCATCGCCGAACAGGGCCTGCGCCGGGCGCGTGAGGAGGAGCGGCGCAGCCTGCCCGCCA
GCACCCGCGACGGCCACCGGGTCGAGATCGCCGCCAATGTCAATTTCGCCGATCAGGCCGCCTTCGCCCTGTCGCAAGGC
GCCGAGGGCGTCGGGCTGATGCGCACGGAATTCCTGTTCCTGGAACGCGGCGATACCCCCGACGAAGAGGCGCAACTGGC
GGCCTATACGGCCATGCAAGACGCGCTGAACGGCCGGCCGCTGATCGTGCGGGCGCTCGATATCGGCGGTGACAAGCAGG
TGGCCCACCTGGATCTGCCGCGCGAGGACAATCCCTTCCTGGGCGTGCGCGGCGCCCGCCTGTTGCTGCGCCGCCCCGAT
CTGCTCGATCCGCAACTGCGCGCCCTCTATCGGGCGGCCAAGAGCGGCAAGCCCTTGTCGATCATGTTCCCGATGATCAC
CTCGGTTCCCGAGATCATCGCCCTGCGCGGCATCTGCGAGCGCATCCGCGCCGAGGTCGAGGGACCGGAGGTGGCTTTGG
GCATCATGGTCGAGGTTCCCGCCGCCGCCCTGGCCGCCGATGTGCTGGCCCGCCACGCCGATTTCTTCTCGATCGGCACC
AATGACTTGACCCAATACACCCTGGCCATCGACCGCCAGAACCCCGAACTGGCGGCCGAGGCCGATAGCCTGCATCCGGC
CGTGCTGCGCTTGATCCGCATGACCGTTGATGGCGCGGCCGCCCATGGGCGGTGGGTCGGCATCTGCGGCGGCATCGCTG
GCGATCCCTTTGGCGCCGCCCTGCTGACCGGGCTGGGGGTGCAGGAATTGTCGATGACGCCGCGCGATATCCCGGCGGTC
AAGGAACGCCTGCGCGATTGCGACGCCACGGCCTTGCGCGCCCTGGCCGAACGCGCCCTGACCTGCGAGACCGCGACCGA
TGTGCGCGCCCTGGAGGCCGACTTGTGA

Upstream 100 bases:

>100_bases
GGAATGAAACCGGCTTTCGCCGGTTTTGCGGAAGCCAAGGGCCGCATCCCAGGTCTGGCAACGGGACCGGCGGCAAGCCG
GACCAGGGAGGAGTGCGCTT

Downstream 100 bases:

>100_bases
TGAGCAGCGATCCCTCCGCCTTGCCCTCCGCCTTGATTGTCCGCACCCTGACCCTTAACCCCGCCATCGATCAGACCATC
ACCCTTGAGGCCCTGACCCC

Product: phosphoenolpyruvate--protein phosphotransferase

Products: NA

Alternate protein names: MTP; Phosphoenolpyruvate-protein phosphotransferase; Phosphotransferase system enzyme I; Phosphocarrier protein HPr; Protein H; Fructose-specific phosphotransferase enzyme IIA component; EIII-Fru; PTS system fructose-specific EIIA component [H]

Number of amino acids: Translated: 835; Mature: 834

Protein sequence:

>835_residues
MGSVDTVGALPRDLVRLAVSPRDKADAIAQAAQMLVAAGCVEAGYAQSMIRREGVANTFLGHGVAIPHGMAEDRALVRAN
GIAVLQVPDGVEWNPGQSARLVVAIAAQSDTHIAILRRLTRLLQDEDRLGGLFVTSDPGDVITALTTDPEVNAPAVPVSD
LAKRFEWVLDYPNGLHARPAARWVDTAKGYAARCQIRHGDEVADVKNLVALLRLGLTQGERISVSAEGPDADQALSRLKA
TMLDLSVQERADAALAARRAKQPVIGWHPPGAPMALAGIAAAPGLAIGRLHVLAAADRSVPDHPVPLSEGGNILHEALSA
TRLQLKALADDTARRLGPSEAGIFKAQGELLNDTDLITLTCQLMVEGHGVAWSWDQAIERMAGKLAALGNALLVARAADL
RDVGRRVLGQLDPGLGGASLHDLPDEPCILIAGDLAPSETAGLDPSRVVGLATAQGGPTAHTAILARTMGLPAMVAAGPG
LLALESGTLAILDGYGGRLYPDPSPEALSSARAFIAEQGLRRAREEERRSLPASTRDGHRVEIAANVNFADQAAFALSQG
AEGVGLMRTEFLFLERGDTPDEEAQLAAYTAMQDALNGRPLIVRALDIGGDKQVAHLDLPREDNPFLGVRGARLLLRRPD
LLDPQLRALYRAAKSGKPLSIMFPMITSVPEIIALRGICERIRAEVEGPEVALGIMVEVPAAALAADVLARHADFFSIGT
NDLTQYTLAIDRQNPELAAEADSLHPAVLRLIRMTVDGAAAHGRWVGICGGIAGDPFGAALLTGLGVQELSMTPRDIPAV
KERLRDCDATALRALAERALTCETATDVRALEADL

Sequences:

>Translated_835_residues
MGSVDTVGALPRDLVRLAVSPRDKADAIAQAAQMLVAAGCVEAGYAQSMIRREGVANTFLGHGVAIPHGMAEDRALVRAN
GIAVLQVPDGVEWNPGQSARLVVAIAAQSDTHIAILRRLTRLLQDEDRLGGLFVTSDPGDVITALTTDPEVNAPAVPVSD
LAKRFEWVLDYPNGLHARPAARWVDTAKGYAARCQIRHGDEVADVKNLVALLRLGLTQGERISVSAEGPDADQALSRLKA
TMLDLSVQERADAALAARRAKQPVIGWHPPGAPMALAGIAAAPGLAIGRLHVLAAADRSVPDHPVPLSEGGNILHEALSA
TRLQLKALADDTARRLGPSEAGIFKAQGELLNDTDLITLTCQLMVEGHGVAWSWDQAIERMAGKLAALGNALLVARAADL
RDVGRRVLGQLDPGLGGASLHDLPDEPCILIAGDLAPSETAGLDPSRVVGLATAQGGPTAHTAILARTMGLPAMVAAGPG
LLALESGTLAILDGYGGRLYPDPSPEALSSARAFIAEQGLRRAREEERRSLPASTRDGHRVEIAANVNFADQAAFALSQG
AEGVGLMRTEFLFLERGDTPDEEAQLAAYTAMQDALNGRPLIVRALDIGGDKQVAHLDLPREDNPFLGVRGARLLLRRPD
LLDPQLRALYRAAKSGKPLSIMFPMITSVPEIIALRGICERIRAEVEGPEVALGIMVEVPAAALAADVLARHADFFSIGT
NDLTQYTLAIDRQNPELAAEADSLHPAVLRLIRMTVDGAAAHGRWVGICGGIAGDPFGAALLTGLGVQELSMTPRDIPAV
KERLRDCDATALRALAERALTCETATDVRALEADL
>Mature_834_residues
GSVDTVGALPRDLVRLAVSPRDKADAIAQAAQMLVAAGCVEAGYAQSMIRREGVANTFLGHGVAIPHGMAEDRALVRANG
IAVLQVPDGVEWNPGQSARLVVAIAAQSDTHIAILRRLTRLLQDEDRLGGLFVTSDPGDVITALTTDPEVNAPAVPVSDL
AKRFEWVLDYPNGLHARPAARWVDTAKGYAARCQIRHGDEVADVKNLVALLRLGLTQGERISVSAEGPDADQALSRLKAT
MLDLSVQERADAALAARRAKQPVIGWHPPGAPMALAGIAAAPGLAIGRLHVLAAADRSVPDHPVPLSEGGNILHEALSAT
RLQLKALADDTARRLGPSEAGIFKAQGELLNDTDLITLTCQLMVEGHGVAWSWDQAIERMAGKLAALGNALLVARAADLR
DVGRRVLGQLDPGLGGASLHDLPDEPCILIAGDLAPSETAGLDPSRVVGLATAQGGPTAHTAILARTMGLPAMVAAGPGL
LALESGTLAILDGYGGRLYPDPSPEALSSARAFIAEQGLRRAREEERRSLPASTRDGHRVEIAANVNFADQAAFALSQGA
EGVGLMRTEFLFLERGDTPDEEAQLAAYTAMQDALNGRPLIVRALDIGGDKQVAHLDLPREDNPFLGVRGARLLLRRPDL
LDPQLRALYRAAKSGKPLSIMFPMITSVPEIIALRGICERIRAEVEGPEVALGIMVEVPAAALAADVLARHADFFSIGTN
DLTQYTLAIDRQNPELAAEADSLHPAVLRLIRMTVDGAAAHGRWVGICGGIAGDPFGAALLTGLGVQELSMTPRDIPAVK
ERLRDCDATALRALAERALTCETATDVRALEADL

Specific function: The phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS), a major carbohydrate active -transport system, catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane. This

COG id: COG1080

COG function: function code G; Phosphoenolpyruvate-protein kinase (PTS system EI component in bacteria)

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PTS EIIA type-2 domain [H]

Homologues:

Organism=Escherichia coli, GI1788756, Length=566, Percent_Identity=39.0459363957597, Blast_Score=367, Evalue=1e-102,
Organism=Escherichia coli, GI1788726, Length=681, Percent_Identity=33.1864904552129, Blast_Score=313, Evalue=2e-86,
Organism=Escherichia coli, GI1789193, Length=495, Percent_Identity=37.3737373737374, Blast_Score=300, Evalue=3e-82,
Organism=Escherichia coli, GI48994992, Length=698, Percent_Identity=31.2320916905444, Blast_Score=284, Evalue=2e-77,
Organism=Escherichia coli, GI1788494, Length=104, Percent_Identity=48.0769230769231, Blast_Score=103, Evalue=6e-23,
Organism=Escherichia coli, GI1787994, Length=390, Percent_Identity=27.9487179487179, Blast_Score=97, Evalue=3e-21,
Organism=Escherichia coli, GI226510935, Length=357, Percent_Identity=24.0896358543417, Blast_Score=96, Evalue=7e-21,
Organism=Escherichia coli, GI1790027, Length=124, Percent_Identity=37.0967741935484, Blast_Score=80, Evalue=5e-16,
Organism=Escherichia coli, GI1788755, Length=80, Percent_Identity=41.25, Blast_Score=65, Evalue=2e-11,

Paralogues:

None

Copy number: 360 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2659 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 4,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008279
- InterPro:   IPR006318
- InterPro:   IPR018274
- InterPro:   IPR023151
- InterPro:   IPR000121
- InterPro:   IPR016152
- InterPro:   IPR002178
- InterPro:   IPR001020
- InterPro:   IPR005698
- InterPro:   IPR000032
- InterPro:   IPR002114
- InterPro:   IPR008731
- InterPro:   IPR015813 [H]

Pfam domain/function: PF05524 PEP-utilisers_N; PF00391 PEP-utilizers; PF02896 PEP-utilizers_C; PF00381 PTS-HPr; PF00359 PTS_EIIA_2 [H]

EC number: =2.7.3.9 [H]

Molecular weight: Translated: 88037; Mature: 87906

Theoretical pI: Translated: 5.16; Mature: 5.16

Prosite motif: PS00369 PTS_HPR_HIS ; PS00372 PTS_EIIA_TYPE_2_HIS ; PS51094 PTS_EIIA_TYPE_2 ; PS00370 PEP_ENZYMES_PHOS_SITE ; PS00742 PEP_ENZYMES_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGSVDTVGALPRDLVRLAVSPRDKADAIAQAAQMLVAAGCVEAGYAQSMIRREGVANTFL
CCCCCCCCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHH
GHGVAIPHGMAEDRALVRANGIAVLQVPDGVEWNPGQSARLVVAIAAQSDTHIAILRRLT
CCCCCCCCCCCCCCCEEEECCEEEEECCCCCCCCCCCCCEEEEEEECCCCCHHHHHHHHH
RLLQDEDRLGGLFVTSDPGDVITALTTDPEVNAPAVPVSDLAKRFEWVLDYPNGLHARPA
HHHCCCHHCCCEEEECCCCCEEEEEECCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCH
ARWVDTAKGYAARCQIRHGDEVADVKNLVALLRLGLTQGERISVSAEGPDADQALSRLKA
HHHHHHCCCCEEEEEECCCCHHHHHHHHHHHHHHCCCCCCEEEEECCCCCHHHHHHHHHH
TMLDLSVQERADAALAARRAKQPVIGWHPPGAPMALAGIAAAPGLAIGRLHVLAAADRSV
HHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHCCCCHHHHEEEEEECCCCC
PDHPVPLSEGGNILHEALSATRLQLKALADDTARRLGPSEAGIFKAQGELLNDTDLITLT
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEECCCCCCCCCEEEEE
CQLMVEGHGVAWSWDQAIERMAGKLAALGNALLVARAADLRDVGRRVLGQLDPGLGGASL
EEEEEECCCCEECHHHHHHHHHHHHHHHCCHHEEHHHHHHHHHHHHHHHHCCCCCCCCCC
HDLPDEPCILIAGDLAPSETAGLDPSRVVGLATAQGGPTAHTAILARTMGLPAMVAAGPG
CCCCCCCEEEEECCCCCCCCCCCCHHHEEEEEECCCCCHHHHHHHHHHCCCCEEECCCCC
LLALESGTLAILDGYGGRLYPDPSPEALSSARAFIAEQGLRRAREEERRSLPASTRDGHR
EEEECCCCEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCE
VEIAANVNFADQAAFALSQGAEGVGLMRTEFLFLERGDTPDEEAQLAAYTAMQDALNGRP
EEEEECCCHHHHHHHHHHCCCCCCCEEEEEEEEEECCCCCCHHHHHHHHHHHHHHCCCCE
LIVRALDIGGDKQVAHLDLPREDNPFLGVRGARLLLRRPDLLDPQLRALYRAAKSGKPLS
EEEEEEECCCCCEEEEEECCCCCCCEEECCHHHHEECCCCCCCHHHHHHHHHHHCCCCEE
IMFPMITSVPEIIALRGICERIRAEVEGPEVALGIMVEVPAAALAADVLARHADFFSIGT
EEHHHHHCHHHHHHHHHHHHHHHHHCCCCCEEEEEEEECCHHHHHHHHHHHHCCCEECCC
NDLTQYTLAIDRQNPELAAEADSLHPAVLRLIRMTVDGAAAHGRWVGICGGIAGDPFGAA
CCCEEEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCEEEEECCCCCCCHHHH
LLTGLGVQELSMTPRDIPAVKERLRDCDATALRALAERALTCETATDVRALEADL
HHHCCCHHHHCCCCCCCHHHHHHHHCCCHHHHHHHHHHHHCCCCHHHHHHHHCCC
>Mature Secondary Structure 
GSVDTVGALPRDLVRLAVSPRDKADAIAQAAQMLVAAGCVEAGYAQSMIRREGVANTFL
CCCCCCCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHH
GHGVAIPHGMAEDRALVRANGIAVLQVPDGVEWNPGQSARLVVAIAAQSDTHIAILRRLT
CCCCCCCCCCCCCCCEEEECCEEEEECCCCCCCCCCCCCEEEEEEECCCCCHHHHHHHHH
RLLQDEDRLGGLFVTSDPGDVITALTTDPEVNAPAVPVSDLAKRFEWVLDYPNGLHARPA
HHHCCCHHCCCEEEECCCCCEEEEEECCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCH
ARWVDTAKGYAARCQIRHGDEVADVKNLVALLRLGLTQGERISVSAEGPDADQALSRLKA
HHHHHHCCCCEEEEEECCCCHHHHHHHHHHHHHHCCCCCCEEEEECCCCCHHHHHHHHHH
TMLDLSVQERADAALAARRAKQPVIGWHPPGAPMALAGIAAAPGLAIGRLHVLAAADRSV
HHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHCCCCHHHHEEEEEECCCCC
PDHPVPLSEGGNILHEALSATRLQLKALADDTARRLGPSEAGIFKAQGELLNDTDLITLT
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEECCCCCCCCCEEEEE
CQLMVEGHGVAWSWDQAIERMAGKLAALGNALLVARAADLRDVGRRVLGQLDPGLGGASL
EEEEEECCCCEECHHHHHHHHHHHHHHHCCHHEEHHHHHHHHHHHHHHHHCCCCCCCCCC
HDLPDEPCILIAGDLAPSETAGLDPSRVVGLATAQGGPTAHTAILARTMGLPAMVAAGPG
CCCCCCCEEEEECCCCCCCCCCCCHHHEEEEEECCCCCHHHHHHHHHHCCCCEEECCCCC
LLALESGTLAILDGYGGRLYPDPSPEALSSARAFIAEQGLRRAREEERRSLPASTRDGHR
EEEECCCCEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCE
VEIAANVNFADQAAFALSQGAEGVGLMRTEFLFLERGDTPDEEAQLAAYTAMQDALNGRP
EEEEECCCHHHHHHHHHHCCCCCCCEEEEEEEEEECCCCCCHHHHHHHHHHHHHHCCCCE
LIVRALDIGGDKQVAHLDLPREDNPFLGVRGARLLLRRPDLLDPQLRALYRAAKSGKPLS
EEEEEEECCCCCEEEEEECCCCCCCEEECCHHHHEECCCCCCCHHHHHHHHHHHCCCCEE
IMFPMITSVPEIIALRGICERIRAEVEGPEVALGIMVEVPAAALAADVLARHADFFSIGT
EEHHHHHCHHHHHHHHHHHHHHHHHCCCCCEEEEEEEECCHHHHHHHHHHHHCCCEECCC
NDLTQYTLAIDRQNPELAAEADSLHPAVLRLIRMTVDGAAAHGRWVGICGGIAGDPFGAA
CCCEEEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCEEEEECCCCCCCHHHH
LLTGLGVQELSMTPRDIPAVKERLRDCDATALRALAERALTCETATDVRALEADL
HHHCCCHHHHCCCCCCCHHHHHHHHCCCHHHHHHHHHHHHCCCCHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7496537; 12024217; 1655739 [H]