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The map label for this gene is purA [H]

Identifier: 83593299

GI number: 83593299

Start: 2271383

End: 2272396

Strand: Reverse

Name: purA [H]

Synonym: Rru_A1964

Alternate gene names: 83593299

Gene position: 2272396-2271383 (Counterclockwise)

Preceding gene: 83593305

Following gene: 83593298

Centisome position: 52.21

GC content: 69.43

Gene sequence:

>1014_bases
ATGGTGGGGTGCTGCACCGTGGTGGCCGGCGGCCAGTGGGGGGACGAGGCCAAGGGCAAGATTTCCGCCTATCTCAGCCT
GACCGACGGCATCGACGTGGCGGCGCGCGCCGGTCTGGGGCCCGGGGCCGGCCATACGGTGGTTTCGGCCGGAACCACCT
TGAAATTGCGCCAGCTTCCCGCCGCCGTCGTCCGCCCGCAAACCACCTTGCTGTTGGGGGCCGGGGTGATGGTCAATCCG
GCGGTTTTCCTGGCCGAGGTCGCCGATCTGGGGGCGGGGCGACGGGTTGGACTCGATCCCCGGGCCAGCATCATCGACGA
GGCCCATCGCCGGACCGACCGCGAGGATCCCCATCTGACCACCACCATCGGGTCGACCGGCAGCGGCCATGGCCCCTGTC
TGGCCGATCGCGCCCTACGCCGGGGGCGGCTGGCCGGCGAGGTGGCCGCTCTCGCCCCCTATCTGGCCGATGTCGCCGAA
CTGCTCAATCAGGCCCTTGATGACGGGCGCAAGGTGCTGATCGAAGGCACCAACGGTTTTTTGCTGTCGGTGCTTTACGG
CACCTATCCGCATACGGTGGGCAAGGATTCGACGGCGGCGACCATCGCCGCCGATGTCGGCCTGGGGCCAACCCGCATCA
ATAGGGTGGTTCTGGCCTTCAAGGCCTTTCCCACCCGGGTCGGCGGCGGACCCTTTCCCACCGAATTGCCCCGCGCCGAG
ATCGAGGCGCGCGGCTTCCTTGAACAGGGCACGGTGACCGGCCGGCCGCGCCGGGTTGGCACCTTCGATTTCGCCGCCGC
GGGGCGCGCCGCCCGCATCAACGCCCCGACCGATATCGCCCTGACCTTCCTTGATCGCATCGATCCCGAGTGTCGCGGCC
GGCCGTTTGATCGCCTGAGCCGCGCCGCCCGCGCCTTCATCGACCAGGTCGAAGACGCCTGCCGCGCCCCGGTGACGCTG
ATCGGCACCGGTCCCGATACCTTCGATATCCTGGACCGGCGGGGCGAACGGTGA

Upstream 100 bases:

>100_bases
TCGGTTGAATCCACAAGACTGTCAACGTATAAAGTGTGAACAACGATAATTATCGCTATGCGTGTATTATCGATCGGGGC
GGGTCATCGGGAGAAATGGG

Downstream 100 bases:

>100_bases
TCGGGCGCTATCGCCGGGCCGAGATGGCCGCTGTTTGGGAGCGCGAAAGCCGCTATTGGCTGTGGCTGGAGATCGAAACC
GCCGTGCTGGACGCCCAGGC

Product: adenylosuccinate synthetase

Products: NA

Alternate protein names: AMPSase; AdSS; IMP--aspartate ligase [H]

Number of amino acids: Translated: 337; Mature: 337

Protein sequence:

>337_residues
MVGCCTVVAGGQWGDEAKGKISAYLSLTDGIDVAARAGLGPGAGHTVVSAGTTLKLRQLPAAVVRPQTTLLLGAGVMVNP
AVFLAEVADLGAGRRVGLDPRASIIDEAHRRTDREDPHLTTTIGSTGSGHGPCLADRALRRGRLAGEVAALAPYLADVAE
LLNQALDDGRKVLIEGTNGFLLSVLYGTYPHTVGKDSTAATIAADVGLGPTRINRVVLAFKAFPTRVGGGPFPTELPRAE
IEARGFLEQGTVTGRPRRVGTFDFAAAGRAARINAPTDIALTFLDRIDPECRGRPFDRLSRAARAFIDQVEDACRAPVTL
IGTGPDTFDILDRRGER

Sequences:

>Translated_337_residues
MVGCCTVVAGGQWGDEAKGKISAYLSLTDGIDVAARAGLGPGAGHTVVSAGTTLKLRQLPAAVVRPQTTLLLGAGVMVNP
AVFLAEVADLGAGRRVGLDPRASIIDEAHRRTDREDPHLTTTIGSTGSGHGPCLADRALRRGRLAGEVAALAPYLADVAE
LLNQALDDGRKVLIEGTNGFLLSVLYGTYPHTVGKDSTAATIAADVGLGPTRINRVVLAFKAFPTRVGGGPFPTELPRAE
IEARGFLEQGTVTGRPRRVGTFDFAAAGRAARINAPTDIALTFLDRIDPECRGRPFDRLSRAARAFIDQVEDACRAPVTL
IGTGPDTFDILDRRGER
>Mature_337_residues
MVGCCTVVAGGQWGDEAKGKISAYLSLTDGIDVAARAGLGPGAGHTVVSAGTTLKLRQLPAAVVRPQTTLLLGAGVMVNP
AVFLAEVADLGAGRRVGLDPRASIIDEAHRRTDREDPHLTTTIGSTGSGHGPCLADRALRRGRLAGEVAALAPYLADVAE
LLNQALDDGRKVLIEGTNGFLLSVLYGTYPHTVGKDSTAATIAADVGLGPTRINRVVLAFKAFPTRVGGGPFPTELPRAE
IEARGFLEQGTVTGRPRRVGTFDFAAAGRAARINAPTDIALTFLDRIDPECRGRPFDRLSRAARAFIDQVEDACRAPVTL
IGTGPDTFDILDRRGER

Specific function: Plays an important role in the de novo pathway of purine nucleotide biosynthesis. Catalyzes the first commited step in the biosynthesis of AMP from IMP [H]

COG id: COG0104

COG function: function code F; Adenylosuccinate synthase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the adenylosuccinate synthetase family [H]

Homologues:

Organism=Homo sapiens, GI34577063, Length=340, Percent_Identity=29.4117647058824, Blast_Score=130, Evalue=1e-30,
Organism=Homo sapiens, GI22748717, Length=339, Percent_Identity=29.7935103244838, Blast_Score=124, Evalue=9e-29,
Organism=Homo sapiens, GI40316944, Length=304, Percent_Identity=27.6315789473684, Blast_Score=100, Evalue=3e-21,
Organism=Escherichia coli, GI1790620, Length=421, Percent_Identity=29.9287410926366, Blast_Score=152, Evalue=4e-38,
Organism=Caenorhabditis elegans, GI25146553, Length=425, Percent_Identity=29.8823529411765, Blast_Score=168, Evalue=5e-42,
Organism=Caenorhabditis elegans, GI25146555, Length=425, Percent_Identity=29.8823529411765, Blast_Score=167, Evalue=6e-42,
Organism=Saccharomyces cerevisiae, GI6324109, Length=424, Percent_Identity=25.9433962264151, Blast_Score=143, Evalue=4e-35,
Organism=Drosophila melanogaster, GI21356033, Length=339, Percent_Identity=31.2684365781711, Blast_Score=146, Evalue=2e-35,

Paralogues:

None

Copy number: 940 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018220
- InterPro:   IPR001114 [H]

Pfam domain/function: PF00709 Adenylsucc_synt [H]

EC number: =6.3.4.4 [H]

Molecular weight: Translated: 35364; Mature: 35364

Theoretical pI: Translated: 8.22; Mature: 8.22

Prosite motif: PS01266 ADENYLOSUCCIN_SYN_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
0.6 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
0.6 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVGCCTVVAGGQWGDEAKGKISAYLSLTDGIDVAARAGLGPGAGHTVVSAGTTLKLRQLP
CCEEEEEEECCCCCCCCCCEEEEEEEECCCCCHHHHCCCCCCCCCEEEECCCEEEHHHCC
AAVVRPQTTLLLGAGVMVNPAVFLAEVADLGAGRRVGLDPRASIIDEAHRRTDREDPHLT
HHHHCCCEEEEEECCHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHCCCCCCCEE
TTIGSTGSGHGPCLADRALRRGRLAGEVAALAPYLADVAELLNQALDDGRKVLIEGTNGF
EEECCCCCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCE
LLSVLYGTYPHTVGKDSTAATIAADVGLGPTRINRVVLAFKAFPTRVGGGPFPTELPRAE
EEEEHHCCCCCCCCCCCCCEEEEECCCCCHHHHHHHHEEEECCCCCCCCCCCCCCCCCHH
IEARGFLEQGTVTGRPRRVGTFDFAAAGRAARINAPTDIALTFLDRIDPECRGRPFDRLS
HHHHCCHHCCCCCCCCCCCCCCCHHHCCCEEECCCCHHHHHHHHHHCCHHHCCCCHHHHH
RAARAFIDQVEDACRAPVTLIGTGPDTFDILDRRGER
HHHHHHHHHHHHHHCCCEEEEECCCCHHHHHHCCCCC
>Mature Secondary Structure
MVGCCTVVAGGQWGDEAKGKISAYLSLTDGIDVAARAGLGPGAGHTVVSAGTTLKLRQLP
CCEEEEEEECCCCCCCCCCEEEEEEEECCCCCHHHHCCCCCCCCCEEEECCCEEEHHHCC
AAVVRPQTTLLLGAGVMVNPAVFLAEVADLGAGRRVGLDPRASIIDEAHRRTDREDPHLT
HHHHCCCEEEEEECCHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHCCCCCCCEE
TTIGSTGSGHGPCLADRALRRGRLAGEVAALAPYLADVAELLNQALDDGRKVLIEGTNGF
EEECCCCCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCE
LLSVLYGTYPHTVGKDSTAATIAADVGLGPTRINRVVLAFKAFPTRVGGGPFPTELPRAE
EEEEHHCCCCCCCCCCCCCEEEEECCCCCHHHHHHHHEEEECCCCCCCCCCCCCCCCCHH
IEARGFLEQGTVTGRPRRVGTFDFAAAGRAARINAPTDIALTFLDRIDPECRGRPFDRLS
HHHHCCHHCCCCCCCCCCCCCCCHHHCCCEEECCCCHHHHHHHHHHCCHHHCCCCHHHHH
RAARAFIDQVEDACRAPVTLIGTGPDTFDILDRRGER
HHHHHHHHHHHHHHCCCEEEEECCCCHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA