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The map label for this gene is purB [H]

Identifier: 83593298

GI number: 83593298

Start: 2270085

End: 2271386

Strand: Reverse

Name: purB [H]

Synonym: Rru_A1963

Alternate gene names: 83593298

Gene position: 2271386-2270085 (Counterclockwise)

Preceding gene: 83593299

Following gene: 83593295

Centisome position: 52.18

GC content: 68.59

Gene sequence:

>1302_bases
GTGATCGGGCGCTATCGCCGGGCCGAGATGGCCGCTGTTTGGGAGCGCGAAAGCCGCTATTGGCTGTGGCTGGAGATCGA
AACCGCCGTGCTGGACGCCCAGGCGGCCCTGGGCGTGGTGCCGGCCGAGGCCGCCCTGGCCGTCCGCCGCGCCCGCATCG
ACAGCGCGGCGATCGACCGGCTGGAAGCCACCCTGCGCCACGAGGTGATCGCCTTCCTGACCCATATCGCCGACCAGATG
GGCGAGGAGGGGCGCTATCTCCATTACGGCCTGACCTCGTCGGATATCCTTGATACGGCGCTAGCCTTGCAACTAACCCG
GGCCGGCGCGATCCTTGAGGACGGGTTGGCGCGGATGATCGCCGCCCTGGAGGACCAGGTCCGCCGCCACCGCCGCACGC
CCTGCCTGGGGCGGACCCATGGCATGGCCGCCGAGCCCACCACCTTCGGCTTGAAACTGGCTGGCCATCTTGCCGAATTC
CAGCGGGCGCGGACGCGGCTGGCCTGGGCCTTGCGGGAAATCGCCGTATGCAAGATCGCCGGCGCCGTCGGCACTTATGC
CACCGTCGATCCGAAGGTCGAGGCGCAAGTCGCCGCGCAGTTCGGGCTGGCGGTGGAAACGGCGGCCACCCAGGTGGTGC
CGCGCGATCGCCACGCGGTTTTCTTCGCCGTCCTTGCCGTCATCGCCGGCGCCGTCGAGCGGCTGGCCACCGAGATCCGT
AATCTGCAGCGCATCGAGATCGGCGAGGTCGAGGAGCCCTTTCCCAGCGGCCAGAAGGGATCCTCGGCCATGCCCCATAA
GCGCAATCCGCTGTTTTGCGAAACACTCTGCGGCTTGGCCCGCGTGGTGCGCGCCGGCGTCGTCCCGGCGCTTGAAAACA
TGGTGCTGTGGCATGAGCGCGATATGAGCCATTCCTCGGTCGAACGGGTGGTGGCGCCCGATACCACCCTTGTCCTTGAT
TACGCCCTGCACAGTCTGGCCGCGATCGTGGAGGGCCTGACCGTCCATCCCGAGCGCATGGCCGAGAATCTGGCGCGCTA
TGGCCAAAGCTGCTGGTCGCAAAAGGCCCTGCTCGTTTTGGTGGGCGCCGGCATGAGCCGCGAGGCCGCCTATCGGTTGG
TCCAGGGCCACGCCCTGGCGGCGGCGACGGGCGGCGCCTCGTTCCTGGAGCGGCTGCTTGCCGATCCCGAGCTGCCCCAG
GCGATAACCCCCGACACCCTGGCCGGGCTTGATGACCCGCTGGCCTTCCTCGATCACGCCGCTATCGCCATCGACCGCGC
CCTGGGGGGAGAGGAAGGTTAG

Upstream 100 bases:

>100_bases
GCGCCTTCATCGACCAGGTCGAAGACGCCTGCCGCGCCCCGGTGACGCTGATCGGCACCGGTCCCGATACCTTCGATATC
CTGGACCGGCGGGGCGAACG

Downstream 100 bases:

>100_bases
GCCGTGGACTCATTACGGCTGACACCAAATCCGCGGGGCGACGAGTTTAATGGCTGCAAGGACATTGAGCGGGTCTTTGT
CGTAACGAGTGGCAATACCC

Product: adenylosuccinate lyase

Products: NA

Alternate protein names: ASL; Adenylosuccinase; ASase [H]

Number of amino acids: Translated: 433; Mature: 433

Protein sequence:

>433_residues
MIGRYRRAEMAAVWERESRYWLWLEIETAVLDAQAALGVVPAEAALAVRRARIDSAAIDRLEATLRHEVIAFLTHIADQM
GEEGRYLHYGLTSSDILDTALALQLTRAGAILEDGLARMIAALEDQVRRHRRTPCLGRTHGMAAEPTTFGLKLAGHLAEF
QRARTRLAWALREIAVCKIAGAVGTYATVDPKVEAQVAAQFGLAVETAATQVVPRDRHAVFFAVLAVIAGAVERLATEIR
NLQRIEIGEVEEPFPSGQKGSSAMPHKRNPLFCETLCGLARVVRAGVVPALENMVLWHERDMSHSSVERVVAPDTTLVLD
YALHSLAAIVEGLTVHPERMAENLARYGQSCWSQKALLVLVGAGMSREAAYRLVQGHALAAATGGASFLERLLADPELPQ
AITPDTLAGLDDPLAFLDHAAIAIDRALGGEEG

Sequences:

>Translated_433_residues
MIGRYRRAEMAAVWERESRYWLWLEIETAVLDAQAALGVVPAEAALAVRRARIDSAAIDRLEATLRHEVIAFLTHIADQM
GEEGRYLHYGLTSSDILDTALALQLTRAGAILEDGLARMIAALEDQVRRHRRTPCLGRTHGMAAEPTTFGLKLAGHLAEF
QRARTRLAWALREIAVCKIAGAVGTYATVDPKVEAQVAAQFGLAVETAATQVVPRDRHAVFFAVLAVIAGAVERLATEIR
NLQRIEIGEVEEPFPSGQKGSSAMPHKRNPLFCETLCGLARVVRAGVVPALENMVLWHERDMSHSSVERVVAPDTTLVLD
YALHSLAAIVEGLTVHPERMAENLARYGQSCWSQKALLVLVGAGMSREAAYRLVQGHALAAATGGASFLERLLADPELPQ
AITPDTLAGLDDPLAFLDHAAIAIDRALGGEEG
>Mature_433_residues
MIGRYRRAEMAAVWERESRYWLWLEIETAVLDAQAALGVVPAEAALAVRRARIDSAAIDRLEATLRHEVIAFLTHIADQM
GEEGRYLHYGLTSSDILDTALALQLTRAGAILEDGLARMIAALEDQVRRHRRTPCLGRTHGMAAEPTTFGLKLAGHLAEF
QRARTRLAWALREIAVCKIAGAVGTYATVDPKVEAQVAAQFGLAVETAATQVVPRDRHAVFFAVLAVIAGAVERLATEIR
NLQRIEIGEVEEPFPSGQKGSSAMPHKRNPLFCETLCGLARVVRAGVVPALENMVLWHERDMSHSSVERVVAPDTTLVLD
YALHSLAAIVEGLTVHPERMAENLARYGQSCWSQKALLVLVGAGMSREAAYRLVQGHALAAATGGASFLERLLADPELPQ
AITPDTLAGLDDPLAFLDHAAIAIDRALGGEEG

Specific function: De novo purine biosynthesis; eighth step. [C]

COG id: COG0015

COG function: function code F; Adenylosuccinate lyase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the lyase 1 family. Adenylosuccinate lyase subfamily [H]

Homologues:

Organism=Homo sapiens, GI4557269, Length=457, Percent_Identity=27.3522975929978, Blast_Score=130, Evalue=3e-30,
Organism=Homo sapiens, GI183227688, Length=388, Percent_Identity=28.3505154639175, Blast_Score=124, Evalue=2e-28,
Organism=Escherichia coli, GI1787376, Length=344, Percent_Identity=28.7790697674419, Blast_Score=86, Evalue=4e-18,
Organism=Escherichia coli, GI1790398, Length=274, Percent_Identity=27.3722627737226, Blast_Score=66, Evalue=5e-12,
Organism=Caenorhabditis elegans, GI17508577, Length=429, Percent_Identity=26.1072261072261, Blast_Score=95, Evalue=7e-20,
Organism=Caenorhabditis elegans, GI32564234, Length=353, Percent_Identity=25.7790368271955, Blast_Score=74, Evalue=1e-13,
Organism=Saccharomyces cerevisiae, GI6323391, Length=445, Percent_Identity=28.0898876404494, Blast_Score=128, Evalue=2e-30,
Organism=Drosophila melanogaster, GI24647570, Length=387, Percent_Identity=25.8397932816537, Blast_Score=102, Evalue=4e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR019468
- InterPro:   IPR003031
- InterPro:   IPR000362
- InterPro:   IPR020557
- InterPro:   IPR008948
- InterPro:   IPR022761
- InterPro:   IPR004769 [H]

Pfam domain/function: PF10397 ADSL_C; PF00206 Lyase_1 [H]

EC number: =4.3.2.2 [H]

Molecular weight: Translated: 46940; Mature: 46940

Theoretical pI: Translated: 6.16; Mature: 6.16

Prosite motif: PS00163 FUMARATE_LYASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIGRYRRAEMAAVWERESRYWLWLEIETAVLDAQAALGVVPAEAALAVRRARIDSAAIDR
CCCCHHHHHHHHHHHCCCCEEEEEEEEHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHH
LEATLRHEVIAFLTHIADQMGEEGRYLHYGLTSSDILDTALALQLTRAGAILEDGLARMI
HHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AALEDQVRRHRRTPCLGRTHGMAAEPTTFGLKLAGHLAEFQRARTRLAWALREIAVCKIA
HHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GAVGTYATVDPKVEAQVAAQFGLAVETAATQVVPRDRHAVFFAVLAVIAGAVERLATEIR
HHHCCCEECCCCHHHHHHHHHCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
NLQRIEIGEVEEPFPSGQKGSSAMPHKRNPLFCETLCGLARVVRAGVVPALENMVLWHER
HCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHC
DMSHSSVERVVAPDTTLVLDYALHSLAAIVEGLTVHPERMAENLARYGQSCWSQKALLVL
CCCHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCEEEE
VGAGMSREAAYRLVQGHALAAATGGASFLERLLADPELPQAITPDTLAGLDDPLAFLDHA
ECCCCCHHHHHHHHHCCHHEECCCCHHHHHHHHCCCCCCCCCCCHHHHCCCCHHHHHHHH
AIAIDRALGGEEG
HHHHHHHCCCCCC
>Mature Secondary Structure
MIGRYRRAEMAAVWERESRYWLWLEIETAVLDAQAALGVVPAEAALAVRRARIDSAAIDR
CCCCHHHHHHHHHHHCCCCEEEEEEEEHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHH
LEATLRHEVIAFLTHIADQMGEEGRYLHYGLTSSDILDTALALQLTRAGAILEDGLARMI
HHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AALEDQVRRHRRTPCLGRTHGMAAEPTTFGLKLAGHLAEFQRARTRLAWALREIAVCKIA
HHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GAVGTYATVDPKVEAQVAAQFGLAVETAATQVVPRDRHAVFFAVLAVIAGAVERLATEIR
HHHCCCEECCCCHHHHHHHHHCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
NLQRIEIGEVEEPFPSGQKGSSAMPHKRNPLFCETLCGLARVVRAGVVPALENMVLWHER
HCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHC
DMSHSSVERVVAPDTTLVLDYALHSLAAIVEGLTVHPERMAENLARYGQSCWSQKALLVL
CCCHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCEEEE
VGAGMSREAAYRLVQGHALAAATGGASFLERLLADPELPQAITPDTLAGLDDPLAFLDHA
ECCCCCHHHHHHHHHCCHHEECCCCHHHHHHHHCCCCCCCCCCCHHHHCCCCHHHHHHHH
AIAIDRALGGEEG
HHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8905231 [H]