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The map label for this gene is folE [H]

Identifier: 83593167

GI number: 83593167

Start: 2129033

End: 2129665

Strand: Reverse

Name: folE [H]

Synonym: Rru_A1832

Alternate gene names: 83593167

Gene position: 2129665-2129033 (Counterclockwise)

Preceding gene: 83593168

Following gene: 83593166

Centisome position: 48.93

GC content: 64.61

Gene sequence:

>633_bases
ATGGAAAAGACCTTGCTGTCGCGTGCGCAAAGCGGTGTGGAAGCCTTTGATGCGACGCCGTATTACAAGCCCGTCTCGGT
CGGAGAGGCCGAGGCGGCCATCGCCGTGCTTCTGCGCTATATCGGCGAGGATCCGACGCGCGAGGGATTGGTTGAAACCC
CGGCCCGCTTCATTCGCGCCTGCGGCCATCATTTCGCCGGCTATGCCCAAGATCCCCTGCGCATCCTCGCCACCACCTTT
TCCGAAAGCGCCGGCTACGCCGGGATGATCCTGCTGCGCGACATCCGCTTTGAGGCCTTTTGCGAGCACCATTTGGCCCC
GATCATCGGCAAGGCCCATGTCGCCTATATGCCGAACGACCGCGTGGTCGGGCTGTCGAAGCTCGCCCGGGTTGTCGATG
TCTTCGCCAAGCGCCTGCAACTGCAAGAGCGCATGACCATGCAGATCGCCACGGCGATCGAGGCCGCCCTGGCGCCGCGC
GGGGTGGCGGTGCTGATCGAGTCCGAGCATCACTGCATGAGCACGCGCGGCGCCTATAAGCCGGGATCGACCATGGTGAC
CTCGGTGACGACCGGTTGCTTCGAAGCCGACGGCGCGCCGCGACGGGAGTTTTTCGAGGCCGTTCGCGCCTGA

Upstream 100 bases:

>100_bases
CTTGAGCGCGCCTCTCTCGCCCACTCGTTCCGCCCCGTTCCCTTCCCCTCTCGCCAGGATCGATCCACCGCCTGTCCCTT
CGTGAACGGAGCCGACGCCC

Downstream 100 bases:

>100_bases
CCGACGCGGCGCCTTGCGCCGTTTTCCATCTTTCTGAGGTCGAGGTCAGCCAATGTTTGAAATAACCCGAAAAATCGAAA
TAGACGCCGGCCATCGCATT

Product: GTP cyclohydrolase

Products: NA

Alternate protein names: GTP cyclohydrolase I; GTP-CH-I [H]

Number of amino acids: Translated: 210; Mature: 210

Protein sequence:

>210_residues
MEKTLLSRAQSGVEAFDATPYYKPVSVGEAEAAIAVLLRYIGEDPTREGLVETPARFIRACGHHFAGYAQDPLRILATTF
SESAGYAGMILLRDIRFEAFCEHHLAPIIGKAHVAYMPNDRVVGLSKLARVVDVFAKRLQLQERMTMQIATAIEAALAPR
GVAVLIESEHHCMSTRGAYKPGSTMVTSVTTGCFEADGAPRREFFEAVRA

Sequences:

>Translated_210_residues
MEKTLLSRAQSGVEAFDATPYYKPVSVGEAEAAIAVLLRYIGEDPTREGLVETPARFIRACGHHFAGYAQDPLRILATTF
SESAGYAGMILLRDIRFEAFCEHHLAPIIGKAHVAYMPNDRVVGLSKLARVVDVFAKRLQLQERMTMQIATAIEAALAPR
GVAVLIESEHHCMSTRGAYKPGSTMVTSVTTGCFEADGAPRREFFEAVRA
>Mature_210_residues
MEKTLLSRAQSGVEAFDATPYYKPVSVGEAEAAIAVLLRYIGEDPTREGLVETPARFIRACGHHFAGYAQDPLRILATTF
SESAGYAGMILLRDIRFEAFCEHHLAPIIGKAHVAYMPNDRVVGLSKLARVVDVFAKRLQLQERMTMQIATAIEAALAPR
GVAVLIESEHHCMSTRGAYKPGSTMVTSVTTGCFEADGAPRREFFEAVRA

Specific function: Tetrahydrofolate biosynthesis; first step. [C]

COG id: COG0302

COG function: function code H; GTP cyclohydrolase I

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP cyclohydrolase I family [H]

Homologues:

Organism=Homo sapiens, GI66932968, Length=180, Percent_Identity=43.3333333333333, Blast_Score=157, Evalue=9e-39,
Organism=Homo sapiens, GI4503949, Length=180, Percent_Identity=43.3333333333333, Blast_Score=157, Evalue=9e-39,
Organism=Homo sapiens, GI66932970, Length=147, Percent_Identity=42.1768707482993, Blast_Score=127, Evalue=9e-30,
Organism=Homo sapiens, GI66932972, Length=138, Percent_Identity=44.2028985507246, Blast_Score=125, Evalue=2e-29,
Organism=Escherichia coli, GI1788476, Length=201, Percent_Identity=35.3233830845771, Blast_Score=112, Evalue=1e-26,
Organism=Caenorhabditis elegans, GI17560486, Length=173, Percent_Identity=42.7745664739884, Blast_Score=156, Evalue=6e-39,
Organism=Saccharomyces cerevisiae, GI6321706, Length=174, Percent_Identity=43.6781609195402, Blast_Score=155, Evalue=3e-39,
Organism=Drosophila melanogaster, GI24656782, Length=176, Percent_Identity=42.6136363636364, Blast_Score=140, Evalue=6e-34,
Organism=Drosophila melanogaster, GI24656796, Length=176, Percent_Identity=42.6136363636364, Blast_Score=139, Evalue=1e-33,
Organism=Drosophila melanogaster, GI24656791, Length=176, Percent_Identity=42.6136363636364, Blast_Score=139, Evalue=1e-33,

Paralogues:

None

Copy number: 420 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001474
- InterPro:   IPR020602
- InterPro:   IPR018234 [H]

Pfam domain/function: PF01227 GTP_cyclohydroI [H]

EC number: =3.5.4.16 [H]

Molecular weight: Translated: 22960; Mature: 22960

Theoretical pI: Translated: 7.18; Mature: 7.18

Prosite motif: PS00860 GTP_CYCLOHYDROL_1_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEKTLLSRAQSGVEAFDATPYYKPVSVGEAEAAIAVLLRYIGEDPTREGLVETPARFIRA
CCHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHH
CGHHFAGYAQDPLRILATTFSESAGYAGMILLRDIRFEAFCEHHLAPIIGKAHVAYMPND
HHHHHCCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCC
RVVGLSKLARVVDVFAKRLQLQERMTMQIATAIEAALAPRGVAVLIESEHHCMSTRGAYK
CEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCHHHHCCCCCC
PGSTMVTSVTTGCFEADGAPRREFFEAVRA
CCCCCHHHHHHCCCCCCCCCHHHHHHHHCC
>Mature Secondary Structure
MEKTLLSRAQSGVEAFDATPYYKPVSVGEAEAAIAVLLRYIGEDPTREGLVETPARFIRA
CCHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHH
CGHHFAGYAQDPLRILATTFSESAGYAGMILLRDIRFEAFCEHHLAPIIGKAHVAYMPND
HHHHHCCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCC
RVVGLSKLARVVDVFAKRLQLQERMTMQIATAIEAALAPRGVAVLIESEHHCMSTRGAYK
CEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCHHHHCCCCCC
PGSTMVTSVTTGCFEADGAPRREFFEAVRA
CCCCCHHHHHHCCCCCCCCCHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA