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The map label for this gene is pcm2 [H]

Identifier: 83593134

GI number: 83593134

Start: 2093327

End: 2093977

Strand: Reverse

Name: pcm2 [H]

Synonym: Rru_A1799

Alternate gene names: 83593134

Gene position: 2093977-2093327 (Counterclockwise)

Preceding gene: 83593137

Following gene: 83593133

Centisome position: 48.11

GC content: 63.44

Gene sequence:

>651_bases
ATGGACTACGGCGTCGCACGCACGAACATGATCGAGAACCAGATACGAACCAACCGGGTCACCGATCCGCTGGTCATCGA
GGCGATGGCGGCGGTGCCGCGGGAGATCTTCGTTCCCAAGGCCTTTCGCGGCGTCGCCTATGTCGACGAGGATCTGGCGA
TCGGGGGAGGGCGCTTCCTGCTCGAACCCCTTAATACCGCAAGGCTGCTCCAGGTGGCGGCGATCAAGACATCCGACGTG
GTTCTCGATATCGGCTGCGCCAGCGGGTATTCGTCGGCCGTGCTCGCCCGCATGGCCAGCACCGTCGTCGCCCTGGAATG
CGACGGCGAATTGGCGGCCAAGGCGATGGCCAATCTCGCCGAGCTGGGGCTCGACAACGCCGTGGTGGTCAGCGGACCGC
TGCGGGACGGCTATGCCAAGCAGGCCCCTTACGACGTCATCGTCATCAATGGCGCGATTCCGGCCGTTCCCGCGGCGCTG
AAGCATCAGCTTGCCGATGGCGGTCGGCTGGTGGCGGTGGTCCATGAAAAGGGTTCGGGCCGGGTGAGCGTCACCGAGCG
TCATGGCGATGTCTTTGGCCATCGCATCGCCTTTGATGGAAACTCGCCTTTGCTCAAGGATTTCGAAGAAACCCCCGCCT
TCGTTTTCTGA

Upstream 100 bases:

>100_bases
GTCATGGGGGGGCGGCATCGCTATAACCCGAACGACCCGTCGTTCTCGACTTCCACAGGCGGGTTTCGTGAAGGCCTGAT
TTCCAGAAGGGTGGGTAACC

Downstream 100 bases:

>100_bases
TCCCGTCCATCCCATCCCGTCCGGTGGGCCGCCGATCGGGGGCGGGCGGCCGTCGGTCGGGGGCCTCGGCGTTGGTGTTT
TGCGTTGCACCCCTCTGGTG

Product: protein-L-isoaspartate(D-aspartate) O-methyltransferase

Products: NA

Alternate protein names: L-isoaspartyl protein carboxyl methyltransferase 2; Protein L-isoaspartyl methyltransferase 2; Protein-beta-aspartate methyltransferase 2; PIMT 2 [H]

Number of amino acids: Translated: 216; Mature: 216

Protein sequence:

>216_residues
MDYGVARTNMIENQIRTNRVTDPLVIEAMAAVPREIFVPKAFRGVAYVDEDLAIGGGRFLLEPLNTARLLQVAAIKTSDV
VLDIGCASGYSSAVLARMASTVVALECDGELAAKAMANLAELGLDNAVVVSGPLRDGYAKQAPYDVIVINGAIPAVPAAL
KHQLADGGRLVAVVHEKGSGRVSVTERHGDVFGHRIAFDGNSPLLKDFEETPAFVF

Sequences:

>Translated_216_residues
MDYGVARTNMIENQIRTNRVTDPLVIEAMAAVPREIFVPKAFRGVAYVDEDLAIGGGRFLLEPLNTARLLQVAAIKTSDV
VLDIGCASGYSSAVLARMASTVVALECDGELAAKAMANLAELGLDNAVVVSGPLRDGYAKQAPYDVIVINGAIPAVPAAL
KHQLADGGRLVAVVHEKGSGRVSVTERHGDVFGHRIAFDGNSPLLKDFEETPAFVF
>Mature_216_residues
MDYGVARTNMIENQIRTNRVTDPLVIEAMAAVPREIFVPKAFRGVAYVDEDLAIGGGRFLLEPLNTARLLQVAAIKTSDV
VLDIGCASGYSSAVLARMASTVVALECDGELAAKAMANLAELGLDNAVVVSGPLRDGYAKQAPYDVIVINGAIPAVPAAL
KHQLADGGRLVAVVHEKGSGRVSVTERHGDVFGHRIAFDGNSPLLKDFEETPAFVF

Specific function: Catalyzes the methyl esterification of L-isoaspartyl residues in peptides and proteins that result from spontaneous decomposition of normal L-aspartyl and L-asparaginyl residues. It plays a role in the repair and/or degradation of damaged proteins [H]

COG id: COG2518

COG function: function code O; Protein-L-isoaspartate carboxylmethyltransferase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. L- isoaspartyl/D-aspartyl protein methyltransferase family [H]

Homologues:

Organism=Escherichia coli, GI1789100, Length=184, Percent_Identity=34.7826086956522, Blast_Score=107, Evalue=5e-25,
Organism=Caenorhabditis elegans, GI193207222, Length=182, Percent_Identity=31.8681318681319, Blast_Score=69, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI71983477, Length=188, Percent_Identity=31.9148936170213, Blast_Score=67, Evalue=5e-12,
Organism=Drosophila melanogaster, GI17981723, Length=169, Percent_Identity=31.3609467455621, Blast_Score=68, Evalue=5e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000682 [H]

Pfam domain/function: PF01135 PCMT [H]

EC number: =2.1.1.77 [H]

Molecular weight: Translated: 22873; Mature: 22873

Theoretical pI: Translated: 5.06; Mature: 5.06

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDYGVARTNMIENQIRTNRVTDPLVIEAMAAVPREIFVPKAFRGVAYVDEDLAIGGGRFL
CCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHCCHHHCCCHHHCCCEEECCCEEECCCEEE
LEPLNTARLLQVAAIKTSDVVLDIGCASGYSSAVLARMASTVVALECDGELAAKAMANLA
ECCCCHHHHHHEEEEECCCEEEEEECCCCHHHHHHHHHHHEEEEEEECCHHHHHHHHHHH
ELGLDNAVVVSGPLRDGYAKQAPYDVIVINGAIPAVPAALKHQLADGGRLVAVVHEKGSG
HHCCCCEEEEECCCCCCCCCCCCEEEEEECCCCCHHHHHHHHHHCCCCEEEEEEEECCCC
RVSVTERHGDVFGHRIAFDGNSPLLKDFEETPAFVF
EEEEEECCCCCCCEEEEECCCCCHHHHHHCCCCCCC
>Mature Secondary Structure
MDYGVARTNMIENQIRTNRVTDPLVIEAMAAVPREIFVPKAFRGVAYVDEDLAIGGGRFL
CCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHCCHHHCCCHHHCCCEEECCCEEECCCEEE
LEPLNTARLLQVAAIKTSDVVLDIGCASGYSSAVLARMASTVVALECDGELAAKAMANLA
ECCCCHHHHHHEEEEECCCEEEEEECCCCHHHHHHHHHHHEEEEEEECCHHHHHHHHHHH
ELGLDNAVVVSGPLRDGYAKQAPYDVIVINGAIPAVPAALKHQLADGGRLVAVVHEKGSG
HHCCCCEEEEECCCCCCCCCCCCEEEEEECCCCCHHHHHHHHHHCCCCEEEEEEEECCCC
RVSVTERHGDVFGHRIAFDGNSPLLKDFEETPAFVF
EEEEEECCCCCCCEEEEECCCCCHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA