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The map label for this gene is ntrX [H]

Identifier: 83593015

GI number: 83593015

Start: 1971560

End: 1972960

Strand: Direct

Name: ntrX [H]

Synonym: Rru_A1680

Alternate gene names: 83593015

Gene position: 1971560-1972960 (Clockwise)

Preceding gene: 83593014

Following gene: 83593016

Centisome position: 45.29

GC content: 65.67

Gene sequence:

>1401_bases
ATGGCGCATGACATTCTCATCGTCGATGACGAAGAAGACATCCGCATGATGCTCGCCGGCATCCTGGAGGACGAGGGCTA
CGACACCCGCGTCGCCGTCGATGCCGACAGCTGTTTCAAGGCCATCGACGAGCGCACGCCCGCCGTGGTCATCCTTGATA
TCTGGATGCAGGGCAGCCGGCTTGACGGCCTGCAGGTCCTTGAGGAACTGGCCCGCGATCATCCCTATCTGCCGGTGGTG
ATGATCAGCGGTCATGGCACCATCGAAACCGCCGTCAGCGCCATCAAGCAGGGCGCCTTCGACTTCATCGAAAAGCCCTT
CAAGGCCGACCGCCTGCTGCTGATCATCCAACGCGCCATCGAAACCGCCCAGCTCAAGCTTGAAAACGCCGAACTGCGCA
TGCGCGCCGGCCCCGAGACCCAGATGATCGGAGACTCGGCGCTGATCGGCCATGTGGCGCAAACCATCGCCAAGGTCGCA
CCCACCGGCTCGCGGGTGCTGATCTCCGGGCCGCCGGGAACGGGCAAGGAAGTGGTGGCGCGGATGATCCACGCCCATTC
GCCGCGCGCCCAGAACCGGCTGGTGGTGGTCAATTGCGCCGCCATGCATCCTGACCGCATGGAGATCGAACTGCTCGGCG
CCGAGCCGGGCAGTGGCGACGGCGCCGATTCCTATCGTAAGATCGGCCTGTTCGAACAGGCCGACGGCGGCACCTTGCTG
CTTGACGAGGTCGCCGACATGCCCCTGGAAACCCAGGGCAAGATCGTGCGCATCCTGCAGGACCAAAGCTTCGAGCGGGT
CGGCGGCGGGCGGCGGATCCGTGTCGATGTCCGGGTGATCGCCAGCACCAACCGCGATCTGGCCCAGGAGATCGCCCTTG
GCCGCTTCCGCGAGGATCTCTACTACCGGCTGAACGTCGTGCCGATCCGCCTGCCGGCCCTGCGCGAGCGCCGCGAGGAC
ATCACGCCCTTGGCCCTGCATTTCATGGACCGCGCCGCCTCCAGCGCCGGCCTGCCGCCCCGGCCCTTCGGCGAGGACGC
CCTGGCCGCCTTGCAAAGCTATGACTGGCCGGGCAATGTCCGCCAGCTGCGCAATGTCGTTGACTGGTTGCTGATCATGG
CGCCCGGCGAGGCACGCGAGGCGATCCGCGCCGATATGCTGCCCGCCGAGATCGGTTCGGTCACCCCCGATTCCCTGCGC
CTCGATCGCTCGGGCGAGATCATGACCCTGCCCCTGCGCGAGGCCCGGGAAATGTTCGAGCGCGATTATCTGATCGCCCA
GGTCACGCGCTTTGGCGGCAATGTGTCGAAAACCGCCGAATTCGTTGGCATGGAGCGCTCGGCCCTGCACCGCAAGCTTA
AAAGCCTGGGGGTGGGCGGATCCGATCGCCAGAAGGTATGA

Upstream 100 bases:

>100_bases
GGGGGGGCCGCCATTTCGCTGACACTCCCCCTCACTCCCGTGGTTCGCGCCGCCGACCCCGGGTTCGCATCCCAACCGCC
CCTTCAGGCCGATCCAATCC

Downstream 100 bases:

>100_bases
CCCATTTTGTTCCCTTAAGGACCCTCCGGCCATGAAAGTCATCGTTTGCGGCGCCGGACAGGTCGGCTTTTCCATCGCCC
GCTATCTCGCCGCCGAGAAC

Product: two component, sigma54 specific, Fis family transcriptional regulator

Products: NADP; 2 glutathione [C]

Alternate protein names: NA

Number of amino acids: Translated: 466; Mature: 465

Protein sequence:

>466_residues
MAHDILIVDDEEDIRMMLAGILEDEGYDTRVAVDADSCFKAIDERTPAVVILDIWMQGSRLDGLQVLEELARDHPYLPVV
MISGHGTIETAVSAIKQGAFDFIEKPFKADRLLLIIQRAIETAQLKLENAELRMRAGPETQMIGDSALIGHVAQTIAKVA
PTGSRVLISGPPGTGKEVVARMIHAHSPRAQNRLVVVNCAAMHPDRMEIELLGAEPGSGDGADSYRKIGLFEQADGGTLL
LDEVADMPLETQGKIVRILQDQSFERVGGGRRIRVDVRVIASTNRDLAQEIALGRFREDLYYRLNVVPIRLPALRERRED
ITPLALHFMDRAASSAGLPPRPFGEDALAALQSYDWPGNVRQLRNVVDWLLIMAPGEAREAIRADMLPAEIGSVTPDSLR
LDRSGEIMTLPLREAREMFERDYLIAQVTRFGGNVSKTAEFVGMERSALHRKLKSLGVGGSDRQKV

Sequences:

>Translated_466_residues
MAHDILIVDDEEDIRMMLAGILEDEGYDTRVAVDADSCFKAIDERTPAVVILDIWMQGSRLDGLQVLEELARDHPYLPVV
MISGHGTIETAVSAIKQGAFDFIEKPFKADRLLLIIQRAIETAQLKLENAELRMRAGPETQMIGDSALIGHVAQTIAKVA
PTGSRVLISGPPGTGKEVVARMIHAHSPRAQNRLVVVNCAAMHPDRMEIELLGAEPGSGDGADSYRKIGLFEQADGGTLL
LDEVADMPLETQGKIVRILQDQSFERVGGGRRIRVDVRVIASTNRDLAQEIALGRFREDLYYRLNVVPIRLPALRERRED
ITPLALHFMDRAASSAGLPPRPFGEDALAALQSYDWPGNVRQLRNVVDWLLIMAPGEAREAIRADMLPAEIGSVTPDSLR
LDRSGEIMTLPLREAREMFERDYLIAQVTRFGGNVSKTAEFVGMERSALHRKLKSLGVGGSDRQKV
>Mature_465_residues
AHDILIVDDEEDIRMMLAGILEDEGYDTRVAVDADSCFKAIDERTPAVVILDIWMQGSRLDGLQVLEELARDHPYLPVVM
ISGHGTIETAVSAIKQGAFDFIEKPFKADRLLLIIQRAIETAQLKLENAELRMRAGPETQMIGDSALIGHVAQTIAKVAP
TGSRVLISGPPGTGKEVVARMIHAHSPRAQNRLVVVNCAAMHPDRMEIELLGAEPGSGDGADSYRKIGLFEQADGGTLLL
DEVADMPLETQGKIVRILQDQSFERVGGGRRIRVDVRVIASTNRDLAQEIALGRFREDLYYRLNVVPIRLPALRERREDI
TPLALHFMDRAASSAGLPPRPFGEDALAALQSYDWPGNVRQLRNVVDWLLIMAPGEAREAIRADMLPAEIGSVTPDSLRL
DRSGEIMTLPLREAREMFERDYLIAQVTRFGGNVSKTAEFVGMERSALHRKLKSLGVGGSDRQKV

Specific function: Member of the two-component regulatory system ntrY/ntrX involved in the activation of nitrogen assimilatory genes such as glnA. NtrX is probably phosphorylated by ntrY and interacts with sigma-54 [H]

COG id: COG2204

COG function: function code T; Response regulator containing CheY-like receiver, AAA-type ATPase, and DNA-binding domains

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 sigma-54 factor interaction domain [H]

Homologues:

Organism=Escherichia coli, GI1790299, Length=472, Percent_Identity=40.0423728813559, Blast_Score=323, Evalue=1e-89,
Organism=Escherichia coli, GI1788550, Length=466, Percent_Identity=38.6266094420601, Blast_Score=316, Evalue=2e-87,
Organism=Escherichia coli, GI1790437, Length=456, Percent_Identity=39.6929824561403, Blast_Score=297, Evalue=1e-81,
Organism=Escherichia coli, GI1788905, Length=468, Percent_Identity=35.6837606837607, Blast_Score=257, Evalue=1e-69,
Organism=Escherichia coli, GI87082117, Length=325, Percent_Identity=40.3076923076923, Blast_Score=209, Evalue=2e-55,
Organism=Escherichia coli, GI1789087, Length=325, Percent_Identity=40.3076923076923, Blast_Score=206, Evalue=2e-54,
Organism=Escherichia coli, GI1789233, Length=325, Percent_Identity=36, Blast_Score=204, Evalue=7e-54,
Organism=Escherichia coli, GI87082152, Length=325, Percent_Identity=40, Blast_Score=204, Evalue=7e-54,
Organism=Escherichia coli, GI1787583, Length=320, Percent_Identity=36.25, Blast_Score=190, Evalue=2e-49,
Organism=Escherichia coli, GI1786524, Length=326, Percent_Identity=35.5828220858896, Blast_Score=186, Evalue=4e-48,
Organism=Escherichia coli, GI87081872, Length=331, Percent_Identity=34.7432024169184, Blast_Score=171, Evalue=1e-43,
Organism=Escherichia coli, GI1789828, Length=220, Percent_Identity=35.9090909090909, Blast_Score=121, Evalue=1e-28,
Organism=Escherichia coli, GI87081858, Length=296, Percent_Identity=31.0810810810811, Blast_Score=113, Evalue=2e-26,
Organism=Escherichia coli, GI1786599, Length=162, Percent_Identity=30.8641975308642, Blast_Score=72, Evalue=5e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR011006
- InterPro:   IPR009057
- InterPro:   IPR002197
- InterPro:   IPR002078
- InterPro:   IPR001789 [H]

Pfam domain/function: PF02954 HTH_8; PF00072 Response_reg; PF00158 Sigma54_activat [H]

EC number: NA

Molecular weight: Translated: 51431; Mature: 51300

Theoretical pI: Translated: 5.18; Mature: 5.18

Prosite motif: PS50110 RESPONSE_REGULATORY ; PS00676 SIGMA54_INTERACT_2 ; PS00688 SIGMA54_INTERACT_3 ; PS50045 SIGMA54_INTERACT_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAHDILIVDDEEDIRMMLAGILEDEGYDTRVAVDADSCFKAIDERTPAVVILDIWMQGSR
CCCCEEEEECCHHHHHHHHHHHCCCCCCCEEEECHHHHHHHHHCCCCEEEEEEEEECCCC
LDGLQVLEELARDHPYLPVVMISGHGTIETAVSAIKQGAFDFIEKPFKADRLLLIIQRAI
CCHHHHHHHHHHCCCCEEEEEEECCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHH
ETAQLKLENAELRMRAGPETQMIGDSALIGHVAQTIAKVAPTGSRVLISGPPGTGKEVVA
HHHHEEEECCEEEEECCCCHHHCCCHHHHHHHHHHHHHHCCCCCEEEEECCCCCHHHHHH
RMIHAHSPRAQNRLVVVNCAAMHPDRMEIELLGAEPGSGDGADSYRKIGLFEQADGGTLL
HHHHCCCCCCCCCEEEEEEECCCCCCEEEEEEECCCCCCCCCCHHHHCCCEEECCCCEEE
LDEVADMPLETQGKIVRILQDQSFERVGGGRRIRVDVRVIASTNRDLAQEIALGRFREDL
EHHHHCCCCCCCCCEEEEECCCCHHHHCCCCEEEEEEEEEECCCHHHHHHHHHHHHHHHH
YYRLNVVPIRLPALRERREDITPLALHFMDRAASSAGLPPRPFGEDALAALQSYDWPGNV
EEEEEEEEEECHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHCCCCCCHH
RQLRNVVDWLLIMAPGEAREAIRADMLPAEIGSVTPDSLRLDRSGEIMTLPLREAREMFE
HHHHHHHHHHEEECCCHHHHHHHHHCCHHHHCCCCCCCEEECCCCCEEEECHHHHHHHHH
RDYLIAQVTRFGGNVSKTAEFVGMERSALHRKLKSLGVGGSDRQKV
HHHHHHHHHHHCCCHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure 
AHDILIVDDEEDIRMMLAGILEDEGYDTRVAVDADSCFKAIDERTPAVVILDIWMQGSR
CCCEEEEECCHHHHHHHHHHHCCCCCCCEEEECHHHHHHHHHCCCCEEEEEEEEECCCC
LDGLQVLEELARDHPYLPVVMISGHGTIETAVSAIKQGAFDFIEKPFKADRLLLIIQRAI
CCHHHHHHHHHHCCCCEEEEEEECCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHH
ETAQLKLENAELRMRAGPETQMIGDSALIGHVAQTIAKVAPTGSRVLISGPPGTGKEVVA
HHHHEEEECCEEEEECCCCHHHCCCHHHHHHHHHHHHHHCCCCCEEEEECCCCCHHHHHH
RMIHAHSPRAQNRLVVVNCAAMHPDRMEIELLGAEPGSGDGADSYRKIGLFEQADGGTLL
HHHHCCCCCCCCCEEEEEEECCCCCCEEEEEEECCCCCCCCCCHHHHCCCEEECCCCEEE
LDEVADMPLETQGKIVRILQDQSFERVGGGRRIRVDVRVIASTNRDLAQEIALGRFREDL
EHHHHCCCCCCCCCEEEEECCCCHHHHCCCCEEEEEEEEEECCCHHHHHHHHHHHHHHHH
YYRLNVVPIRLPALRERREDITPLALHFMDRAASSAGLPPRPFGEDALAALQSYDWPGNV
EEEEEEEEEECHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHCCCCCCHH
RQLRNVVDWLLIMAPGEAREAIRADMLPAEIGSVTPDSLRLDRSGEIMTLPLREAREMFE
HHHHHHHHHHEEECCCHHHHHHHHHCCHHHHCCCCCCCEEECCCCCEEEECHHHHHHHHH
RDYLIAQVTRFGGNVSKTAEFVGMERSALHRKLKSLGVGGSDRQKV
HHHHHHHHHHHCCCHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NADPH2; oxidized glutathione [C]

Specific reaction: NADPH2 + oxidized glutathione = NADP + 2 glutathione [C]

General reaction: Oxidoreductases; Acting on NADH or NADPH; With a disulfide as acceptor [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1661370 [H]