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The map label for this gene is birA [H]

Identifier: 83592657

GI number: 83592657

Start: 1559816

End: 1560649

Strand: Direct

Name: birA [H]

Synonym: Rru_A1321

Alternate gene names: 83592657

Gene position: 1559816-1560649 (Clockwise)

Preceding gene: 83592656

Following gene: 83592658

Centisome position: 35.83

GC content: 64.63

Gene sequence:

>834_bases
ATGACCGCTCTGCCGTTTCCCCCCGTCTCTGCCGCCGATCTTGTTCGCCTCCCCAAGGTCGAGCGGCGGCAAGGCACCAT
CGGCCGGACCGTCTTCCACCTCGCCGAGGTGGACTCGACCAACCGGTTGATGGGCGGCCTTGCCAGAGACGGCGCCCTCC
CCGGAACCGTGGTGGTCGCCGACCACCAGGTGGCCGGTCGCGGCAAGGGCGAGCGTCCGTGGTTCTCGCGGCCCGGTGAG
GGGCTCTGCCTCTCGATCCTGCTTTCCCCGAAGCGTCCGATCGAGGAAGTGCCCCAGTTGACCCTGGTGATCGCCGTGGC
CGTGGCCGAGGCGATCACCCGGGCCACCGGGGTCCAGCCCCGGGTCAAATGGCCCAATGACCTGTTGGTCGATGGGCGCA
AGCTCTGCGGCATTCTCTGCGAGCTGCTCTCCTCGGCCGATGGCGATGTCCATCACGTGATCGCCGGCATCGGCCTGAAT
ACCGGGCTGTCGGTTGATGATTTCCCCGGCTCGCTGCAAGCGATTGGAACATCGTTGGCGATTGAAAGCGGACGGGCGAT
CGACCGGTTCGCCGTGCTCCCCGTCTTGCTCGACCATCTCGAAGCCTGGGTCGAGCTTTGGGAGCGCGAGGGATTTGCCC
CGGTGCGCGACGCCTGGGTTCGCCTTTCCTGCACCCTTGGCAAGGACATCACCCTCGAGTCCGAAGGAACGCGCTGCCAT
GGCGTCGCGGTGGATCTCGGTATCGATGGAAGTTTGAGTATTCGCGATGAAAGCGGAGAAGTTCACCACTTTCATTATGG
AGAAACGCGTCTCTCTTCGGCTATCGCCGGCTAA

Upstream 100 bases:

>100_bases
GACTAGCGTCCGGTGTCTCTCTCCCCCCGGGCACCGGACGCTCCCCCTTTCGACACTCTGATTACCCTGGTTCCCGTCTC
CTGCCGATAGGACCACTCCG

Downstream 100 bases:

>100_bases
ACGCGTCAGTATAACAAAATAGAGGTCGTTCCATGTCCAAAACTGGTACAGACAAGAAGTGGGACCACCGATCCGTCGAT
ACGGCGGAGCGGCTCGCGGC

Product: biotin--acetyl-CoA-carboxylase ligase

Products: NA

Alternate protein names: Biotin operon repressor; Biotin--[acetyl-CoA-carboxylase] synthetase; Biotin--protein ligase [H]

Number of amino acids: Translated: 277; Mature: 276

Protein sequence:

>277_residues
MTALPFPPVSAADLVRLPKVERRQGTIGRTVFHLAEVDSTNRLMGGLARDGALPGTVVVADHQVAGRGKGERPWFSRPGE
GLCLSILLSPKRPIEEVPQLTLVIAVAVAEAITRATGVQPRVKWPNDLLVDGRKLCGILCELLSSADGDVHHVIAGIGLN
TGLSVDDFPGSLQAIGTSLAIESGRAIDRFAVLPVLLDHLEAWVELWEREGFAPVRDAWVRLSCTLGKDITLESEGTRCH
GVAVDLGIDGSLSIRDESGEVHHFHYGETRLSSAIAG

Sequences:

>Translated_277_residues
MTALPFPPVSAADLVRLPKVERRQGTIGRTVFHLAEVDSTNRLMGGLARDGALPGTVVVADHQVAGRGKGERPWFSRPGE
GLCLSILLSPKRPIEEVPQLTLVIAVAVAEAITRATGVQPRVKWPNDLLVDGRKLCGILCELLSSADGDVHHVIAGIGLN
TGLSVDDFPGSLQAIGTSLAIESGRAIDRFAVLPVLLDHLEAWVELWEREGFAPVRDAWVRLSCTLGKDITLESEGTRCH
GVAVDLGIDGSLSIRDESGEVHHFHYGETRLSSAIAG
>Mature_276_residues
TALPFPPVSAADLVRLPKVERRQGTIGRTVFHLAEVDSTNRLMGGLARDGALPGTVVVADHQVAGRGKGERPWFSRPGEG
LCLSILLSPKRPIEEVPQLTLVIAVAVAEAITRATGVQPRVKWPNDLLVDGRKLCGILCELLSSADGDVHHVIAGIGLNT
GLSVDDFPGSLQAIGTSLAIESGRAIDRFAVLPVLLDHLEAWVELWEREGFAPVRDAWVRLSCTLGKDITLESEGTRCHG
VAVDLGIDGSLSIRDESGEVHHFHYGETRLSSAIAG

Specific function: BirA acts both as a biotin-operon repressor and as the enzyme that synthesizes the corepressor, acetyl-CoA:carbon-dioxide ligase. This protein also activates biotin to form biotinyl-5'- adenylate and transfers the biotin moiety to biotin-accepting protein

COG id: COG0340

COG function: function code H; Biotin-(acetyl-CoA carboxylase) ligase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the biotin--protein ligase family [H]

Homologues:

Organism=Escherichia coli, GI1790408, Length=248, Percent_Identity=32.6612903225806, Blast_Score=111, Evalue=6e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: =6.3.4.15 [H]

Molecular weight: Translated: 29617; Mature: 29486

Theoretical pI: Translated: 5.82; Mature: 5.82

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
0.7 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
0.4 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTALPFPPVSAADLVRLPKVERRQGTIGRTVFHLAEVDSTNRLMGGLARDGALPGTVVVA
CCCCCCCCCCHHHHHHCCCHHHHCCCHHHHHHHHHHCCCHHHHHHHHHHCCCCCCEEEEE
DHQVAGRGKGERPWFSRPGEGLCLSILLSPKRPIEEVPQLTLVIAVAVAEAITRATGVQP
CCEECCCCCCCCCCCCCCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCC
RVKWPNDLLVDGRKLCGILCELLSSADGDVHHVIAGIGLNTGLSVDDFPGSLQAIGTSLA
CCCCCCHHHHCHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCCCCCCCCHHHHHHHHHE
IESGRAIDRFAVLPVLLDHLEAWVELWEREGFAPVRDAWVRLSCTLGKDITLESEGTRCH
ECCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHEEEEEEECCCCEEECCCCCEEE
GVAVDLGIDGSLSIRDESGEVHHFHYGETRLSSAIAG
EEEEEECCCCCEEEECCCCCEEEEECCHHHHHHHHCC
>Mature Secondary Structure 
TALPFPPVSAADLVRLPKVERRQGTIGRTVFHLAEVDSTNRLMGGLARDGALPGTVVVA
CCCCCCCCCHHHHHHCCCHHHHCCCHHHHHHHHHHCCCHHHHHHHHHHCCCCCCEEEEE
DHQVAGRGKGERPWFSRPGEGLCLSILLSPKRPIEEVPQLTLVIAVAVAEAITRATGVQP
CCEECCCCCCCCCCCCCCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCC
RVKWPNDLLVDGRKLCGILCELLSSADGDVHHVIAGIGLNTGLSVDDFPGSLQAIGTSLA
CCCCCCHHHHCHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCCCCCCCCHHHHHHHHHE
IESGRAIDRFAVLPVLLDHLEAWVELWEREGFAPVRDAWVRLSCTLGKDITLESEGTRCH
ECCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHEEEEEEECCCCEEECCCCCEEE
GVAVDLGIDGSLSIRDESGEVHHFHYGETRLSSAIAG
EEEEEECCCCCEEEECCCCCEEEEECCHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7730294 [H]