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The map label for this gene is mdh

Identifier: 83592546

GI number: 83592546

Start: 1427956

End: 1428912

Strand: Direct

Name: mdh

Synonym: Rru_A1210

Alternate gene names: 83592546

Gene position: 1427956-1428912 (Clockwise)

Preceding gene: 83592544

Following gene: 83592547

Centisome position: 32.81

GC content: 64.26

Gene sequence:

>957_bases
ATGGCACGCAAGAAGATCGCCCTTGTCGGCGCCGGCAATATCGGTGGCACGCTTGCCCATCTCATCGGTCTCAAGGAATT
GGGCGATGTGGTGCTCTTCGACATCGCCGAGGGGACTCCTCAGGGCAAGGCTCTGGATATCGCCGAATCCACCCCGATCG
AAGGGGTCGACGCCAGCTACTCGGGGTCCAACGATTACGCCGCGATCAAGGACGCCGATGTGGTGATCGTCACCGCCGGC
GTGCCGCGCAAGCCGGGCATGAGCCGCGACGACCTGATCGGCATCAACGCCAAGGTGATGTCGGCCGTCGGCCAGGGCAT
CCGCGCCAATTGCCCCAACGCCTTCGTCATCTGCATCACCAACCCGCTCGACGCCATGGTCTGGGTGTTGCGCGAGGTTT
CGGGGCTGCCCCACAACAAGGTGGTCGGCATGGCCGGCGTGCTCGACAGCGCCCGCTTCCGCTATTTCCTGTCCGAGGAA
TTCAATGTCTCGGTCAAGGACGTCAACGCCTTCGTGCTCGGCGGCCACGGCGACACCATGGTTCCGCTGCCGCGCTATTC
GACCGTCGCCGGTATTCCGCTGCCCGATCTGGTGAAGATGGGCTGGACCACCCAGGAGAAGCTCGACCAGATCATCCAGC
GCACCCGCGACGGTGGCGCCGAGATCGTCGGCCTGCTCAAGACCGGATCGGCCTTCTATGCCCCGGCCGCCGCCGCCGTG
CAGATGGCCGAAGCCTATCTCAAGGACCAGAAGCGCGTTCTGCCCTGCGCCGCCTGGGTTGAGGGCCAGTATGGTCTGGA
CGGCATCTATGTCGGCGTCCCCACCATCATCGGCGCCGGCGGCATCGAGAAGGTGATCGAGATCGAACTGAACGCCGACG
AAAAGGCGGCGTTCGCCAAATCCGTCGACAGCGTGCGCGGGCTGATCGCCGCGTCGAAGGAACTGATGCCGAAATAA

Upstream 100 bases:

>100_bases
GGACAGGGCCGCCGCTGGCCCCTTCGGGAGCGGCCGTGCGGCGCATCCCTATTTGCAATTTTTTTTGCAAATTCCACTTG
TTTAAATCGAGAGGGTTTCC

Downstream 100 bases:

>100_bases
GCCGCAATAAGCCGCGAAAGCCGGCGGACCAGGAGAGGCGAAGCTCTATGAACATTCATGAGTATCAGGCCAAGCAGGTC
TTGGCCAAGTACGGGGTTCC

Product: malate dehydrogenase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 318; Mature: 317

Protein sequence:

>318_residues
MARKKIALVGAGNIGGTLAHLIGLKELGDVVLFDIAEGTPQGKALDIAESTPIEGVDASYSGSNDYAAIKDADVVIVTAG
VPRKPGMSRDDLIGINAKVMSAVGQGIRANCPNAFVICITNPLDAMVWVLREVSGLPHNKVVGMAGVLDSARFRYFLSEE
FNVSVKDVNAFVLGGHGDTMVPLPRYSTVAGIPLPDLVKMGWTTQEKLDQIIQRTRDGGAEIVGLLKTGSAFYAPAAAAV
QMAEAYLKDQKRVLPCAAWVEGQYGLDGIYVGVPTIIGAGGIEKVIEIELNADEKAAFAKSVDSVRGLIAASKELMPK

Sequences:

>Translated_318_residues
MARKKIALVGAGNIGGTLAHLIGLKELGDVVLFDIAEGTPQGKALDIAESTPIEGVDASYSGSNDYAAIKDADVVIVTAG
VPRKPGMSRDDLIGINAKVMSAVGQGIRANCPNAFVICITNPLDAMVWVLREVSGLPHNKVVGMAGVLDSARFRYFLSEE
FNVSVKDVNAFVLGGHGDTMVPLPRYSTVAGIPLPDLVKMGWTTQEKLDQIIQRTRDGGAEIVGLLKTGSAFYAPAAAAV
QMAEAYLKDQKRVLPCAAWVEGQYGLDGIYVGVPTIIGAGGIEKVIEIELNADEKAAFAKSVDSVRGLIAASKELMPK
>Mature_317_residues
ARKKIALVGAGNIGGTLAHLIGLKELGDVVLFDIAEGTPQGKALDIAESTPIEGVDASYSGSNDYAAIKDADVVIVTAGV
PRKPGMSRDDLIGINAKVMSAVGQGIRANCPNAFVICITNPLDAMVWVLREVSGLPHNKVVGMAGVLDSARFRYFLSEEF
NVSVKDVNAFVLGGHGDTMVPLPRYSTVAGIPLPDLVKMGWTTQEKLDQIIQRTRDGGAEIVGLLKTGSAFYAPAAAAVQ
MAEAYLKDQKRVLPCAAWVEGQYGLDGIYVGVPTIIGAGGIEKVIEIELNADEKAAFAKSVDSVRGLIAASKELMPK

Specific function: Catalyzes the reversible oxidation of malate to oxaloacetate

COG id: COG0039

COG function: function code C; Malate/lactate dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the LDH/MDH superfamily. MDH type 3 family

Homologues:

Organism=Homo sapiens, GI47059044, Length=309, Percent_Identity=31.7152103559871, Blast_Score=170, Evalue=2e-42,
Organism=Homo sapiens, GI221136809, Length=309, Percent_Identity=31.7152103559871, Blast_Score=170, Evalue=2e-42,
Organism=Homo sapiens, GI5031857, Length=319, Percent_Identity=30.7210031347962, Blast_Score=159, Evalue=4e-39,
Organism=Homo sapiens, GI260099723, Length=319, Percent_Identity=30.7210031347962, Blast_Score=158, Evalue=7e-39,
Organism=Homo sapiens, GI9257228, Length=308, Percent_Identity=30.8441558441558, Blast_Score=156, Evalue=3e-38,
Organism=Homo sapiens, GI4504973, Length=308, Percent_Identity=30.8441558441558, Blast_Score=156, Evalue=3e-38,
Organism=Homo sapiens, GI15082234, Length=307, Percent_Identity=27.6872964169381, Blast_Score=150, Evalue=1e-36,
Organism=Homo sapiens, GI291575128, Length=322, Percent_Identity=29.8136645962733, Blast_Score=145, Evalue=4e-35,
Organism=Homo sapiens, GI4557032, Length=322, Percent_Identity=29.8136645962733, Blast_Score=145, Evalue=4e-35,
Organism=Homo sapiens, GI260099727, Length=221, Percent_Identity=32.579185520362, Blast_Score=112, Evalue=3e-25,
Organism=Homo sapiens, GI260099725, Length=200, Percent_Identity=33.5, Blast_Score=111, Evalue=7e-25,
Organism=Homo sapiens, GI207028494, Length=202, Percent_Identity=31.6831683168317, Blast_Score=110, Evalue=2e-24,
Organism=Homo sapiens, GI21735621, Length=296, Percent_Identity=31.4189189189189, Blast_Score=101, Evalue=7e-22,
Organism=Homo sapiens, GI103472011, Length=291, Percent_Identity=22.680412371134, Blast_Score=78, Evalue=9e-15,
Organism=Escherichia coli, GI1789632, Length=328, Percent_Identity=32.3170731707317, Blast_Score=112, Evalue=2e-26,
Organism=Caenorhabditis elegans, GI17535107, Length=273, Percent_Identity=25.2747252747253, Blast_Score=115, Evalue=2e-26,
Organism=Caenorhabditis elegans, GI17554310, Length=317, Percent_Identity=30.9148264984227, Blast_Score=104, Evalue=7e-23,
Organism=Saccharomyces cerevisiae, GI6322765, Length=299, Percent_Identity=30.7692307692308, Blast_Score=105, Evalue=6e-24,
Organism=Saccharomyces cerevisiae, GI6324446, Length=251, Percent_Identity=31.8725099601594, Blast_Score=87, Evalue=3e-18,
Organism=Saccharomyces cerevisiae, GI6320125, Length=235, Percent_Identity=27.6595744680851, Blast_Score=76, Evalue=9e-15,
Organism=Drosophila melanogaster, GI17136226, Length=304, Percent_Identity=27.6315789473684, Blast_Score=136, Evalue=2e-32,
Organism=Drosophila melanogaster, GI45550422, Length=307, Percent_Identity=28.3387622149837, Blast_Score=109, Evalue=3e-24,
Organism=Drosophila melanogaster, GI24647881, Length=314, Percent_Identity=29.2993630573248, Blast_Score=107, Evalue=9e-24,
Organism=Drosophila melanogaster, GI24663599, Length=309, Percent_Identity=29.4498381877023, Blast_Score=100, Evalue=2e-21,
Organism=Drosophila melanogaster, GI24663595, Length=288, Percent_Identity=28.8194444444444, Blast_Score=88, Evalue=8e-18,

Paralogues:

None

Copy number: 2640 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 260 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2380 Molecules/Cell In: Stationary-Phase, Rich-Media (Based on E. coli). 260 Molecules/Cell In: Stationary Phase

Swissprot (AC and ID): MDH_RHORT (Q2RV34)

Other databases:

- EMBL:   CP000230
- RefSeq:   YP_426298.1
- ProteinModelPortal:   Q2RV34
- SMR:   Q2RV34
- STRING:   Q2RV34
- GeneID:   3833708
- GenomeReviews:   CP000230_GR
- KEGG:   rru:Rru_A1210
- NMPDR:   fig|1085.1.peg.3156
- eggNOG:   COG0039
- HOGENOM:   HBG566126
- OMA:   ECTYVEG
- PhylomeDB:   Q2RV34
- ProtClustDB:   PRK06223
- BioCyc:   RRUB269796:RRU_A1210-MONOMER
- GO:   GO:0005488
- GO:   GO:0006096
- HAMAP:   MF_00487
- InterPro:   IPR001557
- InterPro:   IPR022383
- InterPro:   IPR001236
- InterPro:   IPR015955
- InterPro:   IPR011275
- InterPro:   IPR016040
- Gene3D:   G3DSA:3.90.110.10
- Gene3D:   G3DSA:3.40.50.720
- PIRSF:   PIRSF000102
- PRINTS:   PR00086
- TIGRFAMs:   TIGR01763

Pfam domain/function: PF02866 Ldh_1_C; PF00056 Ldh_1_N; SSF56327 Lactate_DH/Glyco_hydro_4_C

EC number: =1.1.1.37

Molecular weight: Translated: 33479; Mature: 33348

Theoretical pI: Translated: 5.20; Mature: 5.20

Prosite motif: NA

Important sites: ACT_SITE 176-176 BINDING 34-34 BINDING 83-83 BINDING 89-89 BINDING 96-96 BINDING 121-121 BINDING 152-152

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MARKKIALVGAGNIGGTLAHLIGLKELGDVVLFDIAEGTPQGKALDIAESTPIEGVDASY
CCCCEEEEEECCCCCHHHHHHHHHHHHCCEEEEEECCCCCCCCEEECCCCCCCCCCCCCC
SGSNDYAAIKDADVVIVTAGVPRKPGMSRDDLIGINAKVMSAVGQGIRANCPNAFVICIT
CCCCCEEEEECCCEEEEECCCCCCCCCCCCCEEECCHHHHHHHCCCCCCCCCCEEEEEEC
NPLDAMVWVLREVSGLPHNKVVGMAGVLDSARFRYFLSEEFNVSVKDVNAFVLGGHGDTM
CHHHHHHHHHHHHCCCCCCHHEEHHHHHHHHHEEEEECCCCEEEEEEEEEEEECCCCCCE
VPLPRYSTVAGIPLPDLVKMGWTTQEKLDQIIQRTRDGGAEIVGLLKTGSAFYAPAAAAV
EECCCCCCCCCCCCHHHHHCCCCCHHHHHHHHHHHCCCCCEEEEEEECCCCHHCHHHHHH
QMAEAYLKDQKRVLPCAAWVEGQYGLDGIYVGVPTIIGAGGIEKVIEIELNADEKAAFAK
HHHHHHHHHCCCCCCHHHEECCCCCCCEEEECHHHHCCCCCCCEEEEEEECCCHHHHHHH
SVDSVRGLIAASKELMPK
HHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
ARKKIALVGAGNIGGTLAHLIGLKELGDVVLFDIAEGTPQGKALDIAESTPIEGVDASY
CCCEEEEEECCCCCHHHHHHHHHHHHCCEEEEEECCCCCCCCEEECCCCCCCCCCCCCC
SGSNDYAAIKDADVVIVTAGVPRKPGMSRDDLIGINAKVMSAVGQGIRANCPNAFVICIT
CCCCCEEEEECCCEEEEECCCCCCCCCCCCCEEECCHHHHHHHCCCCCCCCCCEEEEEEC
NPLDAMVWVLREVSGLPHNKVVGMAGVLDSARFRYFLSEEFNVSVKDVNAFVLGGHGDTM
CHHHHHHHHHHHHCCCCCCHHEEHHHHHHHHHEEEEECCCCEEEEEEEEEEEECCCCCCE
VPLPRYSTVAGIPLPDLVKMGWTTQEKLDQIIQRTRDGGAEIVGLLKTGSAFYAPAAAAV
EECCCCCCCCCCCCHHHHHCCCCCHHHHHHHHHHHCCCCCEEEEEEECCCCHHCHHHHHH
QMAEAYLKDQKRVLPCAAWVEGQYGLDGIYVGVPTIIGAGGIEKVIEIELNADEKAAFAK
HHHHHHHHHCCCCCCHHHEECCCCCCCEEEECHHHHCCCCCCCEEEEEEECCCHHHHHHH
SVDSVRGLIAASKELMPK
HHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA