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The map label for this gene is thrS [H]

Identifier: 83592514

GI number: 83592514

Start: 1394721

End: 1395884

Strand: Direct

Name: thrS [H]

Synonym: Rru_A1178

Alternate gene names: 83592514

Gene position: 1394721-1395884 (Clockwise)

Preceding gene: 83592513

Following gene: 83592515

Centisome position: 32.04

GC content: 73.71

Gene sequence:

>1164_bases
ATGACCTTTTCAGAAGAGAATCCGCCTGATTGCGCGGCCCCGGGTCCGGGTGTCCGGCCGGGGCGCGATCCCGTGGCCCT
TGGCGAAAGCCAGGAGCTTTTCCTGTGGCGCGCCACCGGCGGCGGCAATCCCTATTGGCTGCCGCGCGGGCTGACCTTGC
TGCGGGTGATCGAGGGCGGCTTGCGCGCGTGGCTGGAAGCGGCGGGCTATGCCGAGATCCGCACGCCCCCGCTGGTCGAC
GGCGCCTTTCGCGGCGGGACCTGCGCGGCGCACCGGCTGGTTCATGCCCTGGCGCGGCGGGCCGAGGACGGGCCGCTGCG
GCTTTTCGAACTGGGCGTCTGTCATCGCGGCGGTCCGGGAGAGGGGCTGCTGGGCGGGGCCGCTTTTACCCCCCCGGCGG
TCGGGACCGCTTTCACCACGGATGAAGCCCATGTGTTCTGCCCTGCCGATCACTGTGTCGCCGAGGTGGCGCGGATCGTC
ACCGCCAGCGCCGCGACCCTGGCCGCCTTCGGCTTCCCGGTGATCGGGGTGCGGTGGGGGACGGCGCGGGCCGGCGAGGA
GTGCCCGCTCGCCGCCGGAGCGCGCCGGGCCGGTTATCAGCCCGGTCTCGCCGCCGGGCCGGCGGGGGAGGGGGCGCCGA
CCGGCGGCTCATGGCTGGTCTTCGAGGTCGACGACGGGGCCGGCGGGGCGTGGCCCTGCGGGGGACTCACCTTGGAGGGC
GAGGCCATCGGCGGGGCGGGGGAGCGGGCGATACTGCTCTGCCATCGGCTGTTTGGCAGCCTGGAAGCCGCCGTTGGCTT
GCTGCTGGCGCGCGGGGACGGCTGGTTGGCGCCCTGGCTCGCCCCCCAGCAGGTTCTGCTGGCCGGCACCGCAGCCGAAG
AGGCCATCGTGCGCTCGATCGCCGGCCGTTTCGCCGAGGCCGGCCTGCGCGTTGGTGCCGACCTGGGGGCCGAGTCCCTG
GGCGTCAAGCGGGCCCGCGCCCGGGCTTTGGGCATCCCGGTGGTCGCCAGCCTTGGCCCGCGCGAGGCGTTCAGCAAGAC
CGTGACCCTATCTTGGCCCGATGACCGCAAGGAAACCCTTTATATCGAGGCCGCCTTGGCCCGCTTAAGCGCCTTCTGCG
CGGTGCCCGCCCCACCCGGGCGGAGCAGGGCTCCGGCCGCATGA

Upstream 100 bases:

>100_bases
AAAAAACCCCACGGCCCATCCGACAGGCGGATGGCATCGTGGGCAGACTCCTCTCCCGCTTCCTCTCCGCCCGTCGTAAA
GCCCCGACCGGAGAGAGAGC

Downstream 100 bases:

>100_bases
CCCGCCTCGCCCGCCGGCTTCATCCGCCGGCGGGCGATCGACGGCTCTTGACGATTTTCGCCTGGCAGTGCATGTTTTTG
CCGATTTAGGCAAAAACAGG

Product: anticodon-binding

Products: NA

Alternate protein names: Threonine--tRNA ligase; ThrRS [H]

Number of amino acids: Translated: 387; Mature: 386

Protein sequence:

>387_residues
MTFSEENPPDCAAPGPGVRPGRDPVALGESQELFLWRATGGGNPYWLPRGLTLLRVIEGGLRAWLEAAGYAEIRTPPLVD
GAFRGGTCAAHRLVHALARRAEDGPLRLFELGVCHRGGPGEGLLGGAAFTPPAVGTAFTTDEAHVFCPADHCVAEVARIV
TASAATLAAFGFPVIGVRWGTARAGEECPLAAGARRAGYQPGLAAGPAGEGAPTGGSWLVFEVDDGAGGAWPCGGLTLEG
EAIGGAGERAILLCHRLFGSLEAAVGLLLARGDGWLAPWLAPQQVLLAGTAAEEAIVRSIAGRFAEAGLRVGADLGAESL
GVKRARARALGIPVVASLGPREAFSKTVTLSWPDDRKETLYIEAALARLSAFCAVPAPPGRSRAPAA

Sequences:

>Translated_387_residues
MTFSEENPPDCAAPGPGVRPGRDPVALGESQELFLWRATGGGNPYWLPRGLTLLRVIEGGLRAWLEAAGYAEIRTPPLVD
GAFRGGTCAAHRLVHALARRAEDGPLRLFELGVCHRGGPGEGLLGGAAFTPPAVGTAFTTDEAHVFCPADHCVAEVARIV
TASAATLAAFGFPVIGVRWGTARAGEECPLAAGARRAGYQPGLAAGPAGEGAPTGGSWLVFEVDDGAGGAWPCGGLTLEG
EAIGGAGERAILLCHRLFGSLEAAVGLLLARGDGWLAPWLAPQQVLLAGTAAEEAIVRSIAGRFAEAGLRVGADLGAESL
GVKRARARALGIPVVASLGPREAFSKTVTLSWPDDRKETLYIEAALARLSAFCAVPAPPGRSRAPAA
>Mature_386_residues
TFSEENPPDCAAPGPGVRPGRDPVALGESQELFLWRATGGGNPYWLPRGLTLLRVIEGGLRAWLEAAGYAEIRTPPLVDG
AFRGGTCAAHRLVHALARRAEDGPLRLFELGVCHRGGPGEGLLGGAAFTPPAVGTAFTTDEAHVFCPADHCVAEVARIVT
ASAATLAAFGFPVIGVRWGTARAGEECPLAAGARRAGYQPGLAAGPAGEGAPTGGSWLVFEVDDGAGGAWPCGGLTLEGE
AIGGAGERAILLCHRLFGSLEAAVGLLLARGDGWLAPWLAPQQVLLAGTAAEEAIVRSIAGRFAEAGLRVGADLGAESLG
VKRARARALGIPVVASLGPREAFSKTVTLSWPDDRKETLYIEAALARLSAFCAVPAPPGRSRAPAA

Specific function: Thrs Is Also A Translational Repressor Protein, It Controls The Translation Of Its Own Gene By Binding To Its mRNA. [C]

COG id: COG0441

COG function: function code J; Threonyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Escherichia coli, GI1788013, Length=377, Percent_Identity=25.4641909814324, Blast_Score=115, Evalue=3e-27,

Paralogues:

None

Copy number: 700 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002314
- InterPro:   IPR006195
- InterPro:   IPR004154
- InterPro:   IPR012675
- InterPro:   IPR004095
- InterPro:   IPR012676
- InterPro:   IPR002320
- InterPro:   IPR018163
- InterPro:   IPR012947 [H]

Pfam domain/function: PF03129 HGTP_anticodon; PF02824 TGS; PF00587 tRNA-synt_2b; PF07973 tRNA_SAD [H]

EC number: =6.1.1.3 [H]

Molecular weight: Translated: 39663; Mature: 39531

Theoretical pI: Translated: 6.26; Mature: 6.26

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
0.3 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
0.0 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTFSEENPPDCAAPGPGVRPGRDPVALGESQELFLWRATGGGNPYWLPRGLTLLRVIEGG
CCCCCCCCCCCCCCCCCCCCCCCCEECCCCCEEEEEEECCCCCCEECCCCHHHHHHHHHH
LRAWLEAAGYAEIRTPPLVDGAFRGGTCAAHRLVHALARRAEDGPLRLFELGVCHRGGPG
HHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCCCC
EGLLGGAAFTPPAVGTAFTTDEAHVFCPADHCVAEVARIVTASAATLAAFGFPVIGVRWG
CCCCCCCCCCCCCCCCEEECCCCEEEECHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEC
TARAGEECPLAAGARRAGYQPGLAAGPAGEGAPTGGSWLVFEVDDGAGGAWPCGGLTLEG
CCCCCCCCCCCCCHHHCCCCCCCCCCCCCCCCCCCCCEEEEEEECCCCCCCCCCCEEEEC
EAIGGAGERAILLCHRLFGSLEAAVGLLLARGDGWLAPWLAPQQVLLAGTAAEEAIVRSI
CCCCCCCCHHHHHHHHHHHHHHHHHEEEEECCCCCCCCCCCCCCEEEECCHHHHHHHHHH
AGRFAEAGLRVGADLGAESLGVKRARARALGIPVVASLGPREAFSKTVTLSWPDDRKETL
HHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCEECCCCHHHHCCEEEEECCCCCCCEE
YIEAALARLSAFCAVPAPPGRSRAPAA
EHHHHHHHHHHHCCCCCCCCCCCCCCC
>Mature Secondary Structure 
TFSEENPPDCAAPGPGVRPGRDPVALGESQELFLWRATGGGNPYWLPRGLTLLRVIEGG
CCCCCCCCCCCCCCCCCCCCCCCEECCCCCEEEEEEECCCCCCEECCCCHHHHHHHHHH
LRAWLEAAGYAEIRTPPLVDGAFRGGTCAAHRLVHALARRAEDGPLRLFELGVCHRGGPG
HHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCCCC
EGLLGGAAFTPPAVGTAFTTDEAHVFCPADHCVAEVARIVTASAATLAAFGFPVIGVRWG
CCCCCCCCCCCCCCCCEEECCCCEEEECHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEC
TARAGEECPLAAGARRAGYQPGLAAGPAGEGAPTGGSWLVFEVDDGAGGAWPCGGLTLEG
CCCCCCCCCCCCCHHHCCCCCCCCCCCCCCCCCCCCCEEEEEEECCCCCCCCCCCEEEEC
EAIGGAGERAILLCHRLFGSLEAAVGLLLARGDGWLAPWLAPQQVLLAGTAAEEAIVRSI
CCCCCCCCHHHHHHHHHHHHHHHHHEEEEECCCCCCCCCCCCCCEEEECCHHHHHHHHHH
AGRFAEAGLRVGADLGAESLGVKRARARALGIPVVASLGPREAFSKTVTLSWPDDRKETL
HHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCEECCCCHHHHCCEEEEECCCCCCCEE
YIEAALARLSAFCAVPAPPGRSRAPAA
EHHHHHHHHHHHCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA