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The map label for this gene is purA

Identifier: 83592442

GI number: 83592442

Start: 1299126

End: 1300415

Strand: Direct

Name: purA

Synonym: Rru_A1106

Alternate gene names: 83592442

Gene position: 1299126-1300415 (Clockwise)

Preceding gene: 83592441

Following gene: 83592443

Centisome position: 29.85

GC content: 67.6

Gene sequence:

>1290_bases
ATGTCGAACGTGGCCGTGGTTGGCTCCCAGTGGGGTGACGAGGGCAAGGGCAAGGTGGTCGATTGGCTGGCCGAGCGCGC
CGATGTGGTGGTGCGTTTCCAGGGCGGTCATAACGCCGGCCATACCCTGGTCGTCGATGGGGTGACCTATAAGCTCAGCC
TGCTGCCCTCGGGCGCGGTGCGCGGCAAGCTGTCGATCATCGGCAATGGCGTGGTGGTCGATCCCTGGGCGTTGTTCGAG
GAAATCGAGCGCGTCGCCGCCCAGGGCGTGGTCATCACCCCCGAAGGCCTGCGCATCGCCGAGAACGCCGTGCTGATCTT
GCCGCTGCACGGCAATCTGGACCGGGCGCGCGAAGCCGCCGCCGGCAATGCCAAGATCGGCACCACCGGGCGCGGCATCG
GCCCGGCCTATGAGGACAAGGTGGCGCGCCGCGCCATCCGCCTGTGCGATCTGGCCGACGAAGACGTCTTGCGCTTCAAG
GTCGAGCGCCTGCTGACCCATCACAACGCCCTGCTGCGCGGTCTGGGCGAGCCCGAGTTGGACGCCGACAGCCTGATCAG
CGCCCTGCTGGAGATCGCCCCGCGCATCCTGCCCTTCGCCTCGCCGGTGTGGAAGGTGCTCGACGAGGCGCGCCGCGCCG
GCAAGAAGATCCTGTTCGAGGGCGCCCAGGGCGCCATGCTCGATGTCGATCACGGCACCTACCCTTATGTCACCTCGTCC
AACACAGTCGCCCCCAACGCGGCGACCGGCGCCGGAGTCGGCCCGTCGAGCGTCGGTTTCACGCTTGGCATCACCAAGGC
CTATACCACCCGTGTCGGCGCCGGTCCGTTCCCGACCGAGCTGTTTGACGATATCGGCCGGACCATCGGCGAGCGCGGCC
GCGAATTCGGCACGGTGACCGGCCGTCCGCGCCGCTGCGGCTGGTTCGACGCCGTGCTGGTGCGCCAGTCGGTGCTGGTC
GGTGGCCTGCGCGGCATCGCCCTGACCAAGCTTGATGTGCTGGATGGCCTGGACGAGCTGAAGATCTGCACCGGCTATCG
GCTCGATGGCCAGGTGATCGACCACCTGCCGGCGTCGATGAAGGCGCAGTCGCGGGTCGAGCCGATCTATGAAACGCTGG
AAGGCTGGAAAGACAGCACCTTCGGCGCCCGCTCCTGGGCCGATCTGCCGGCCAAGGCGATCAAATACATCCGTCGCCTG
GAAGAACTGATCGAGGCGCCGGTGGCCCTGCTGTCGACCAGCCCGGAGCGCGAGGACACCATCCTGGTGCGCGATCCCTT
CCTCGATTAA

Upstream 100 bases:

>100_bases
CGGAAGCAAGGACCCCGGGCAGGGGCCGATCGTCGTGGGTGATCCACGGCCGGCCGCCCGGGATTTCCCATGAATTTTTC
TCGAATACGGGATGGATGCG

Downstream 100 bases:

>100_bases
GCGCGCGGTCTTGGCCTATTCGGCTGCGGGCGGGGTCAAAAGACTTCTGAACCGGCGTGCTGTCCGTTAGTCTGGGTCGG
GAGCGGGAACCCGTCCCGCT

Product: adenylosuccinate synthetase

Products: NA

Alternate protein names: AMPSase; AdSS; IMP--aspartate ligase

Number of amino acids: Translated: 429; Mature: 428

Protein sequence:

>429_residues
MSNVAVVGSQWGDEGKGKVVDWLAERADVVVRFQGGHNAGHTLVVDGVTYKLSLLPSGAVRGKLSIIGNGVVVDPWALFE
EIERVAAQGVVITPEGLRIAENAVLILPLHGNLDRAREAAAGNAKIGTTGRGIGPAYEDKVARRAIRLCDLADEDVLRFK
VERLLTHHNALLRGLGEPELDADSLISALLEIAPRILPFASPVWKVLDEARRAGKKILFEGAQGAMLDVDHGTYPYVTSS
NTVAPNAATGAGVGPSSVGFTLGITKAYTTRVGAGPFPTELFDDIGRTIGERGREFGTVTGRPRRCGWFDAVLVRQSVLV
GGLRGIALTKLDVLDGLDELKICTGYRLDGQVIDHLPASMKAQSRVEPIYETLEGWKDSTFGARSWADLPAKAIKYIRRL
EELIEAPVALLSTSPEREDTILVRDPFLD

Sequences:

>Translated_429_residues
MSNVAVVGSQWGDEGKGKVVDWLAERADVVVRFQGGHNAGHTLVVDGVTYKLSLLPSGAVRGKLSIIGNGVVVDPWALFE
EIERVAAQGVVITPEGLRIAENAVLILPLHGNLDRAREAAAGNAKIGTTGRGIGPAYEDKVARRAIRLCDLADEDVLRFK
VERLLTHHNALLRGLGEPELDADSLISALLEIAPRILPFASPVWKVLDEARRAGKKILFEGAQGAMLDVDHGTYPYVTSS
NTVAPNAATGAGVGPSSVGFTLGITKAYTTRVGAGPFPTELFDDIGRTIGERGREFGTVTGRPRRCGWFDAVLVRQSVLV
GGLRGIALTKLDVLDGLDELKICTGYRLDGQVIDHLPASMKAQSRVEPIYETLEGWKDSTFGARSWADLPAKAIKYIRRL
EELIEAPVALLSTSPEREDTILVRDPFLD
>Mature_428_residues
SNVAVVGSQWGDEGKGKVVDWLAERADVVVRFQGGHNAGHTLVVDGVTYKLSLLPSGAVRGKLSIIGNGVVVDPWALFEE
IERVAAQGVVITPEGLRIAENAVLILPLHGNLDRAREAAAGNAKIGTTGRGIGPAYEDKVARRAIRLCDLADEDVLRFKV
ERLLTHHNALLRGLGEPELDADSLISALLEIAPRILPFASPVWKVLDEARRAGKKILFEGAQGAMLDVDHGTYPYVTSSN
TVAPNAATGAGVGPSSVGFTLGITKAYTTRVGAGPFPTELFDDIGRTIGERGREFGTVTGRPRRCGWFDAVLVRQSVLVG
GLRGIALTKLDVLDGLDELKICTGYRLDGQVIDHLPASMKAQSRVEPIYETLEGWKDSTFGARSWADLPAKAIKYIRRLE
ELIEAPVALLSTSPEREDTILVRDPFLD

Specific function: Plays an important role in the de novo pathway of purine nucleotide biosynthesis. Catalyzes the first commited step in the biosynthesis of AMP from IMP

COG id: COG0104

COG function: function code F; Adenylosuccinate synthase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the adenylosuccinate synthetase family

Homologues:

Organism=Homo sapiens, GI22748717, Length=422, Percent_Identity=44.5497630331754, Blast_Score=340, Evalue=1e-93,
Organism=Homo sapiens, GI34577063, Length=425, Percent_Identity=43.0588235294118, Blast_Score=332, Evalue=3e-91,
Organism=Homo sapiens, GI40316944, Length=394, Percent_Identity=42.6395939086294, Blast_Score=299, Evalue=4e-81,
Organism=Escherichia coli, GI1790620, Length=429, Percent_Identity=51.5151515151515, Blast_Score=434, Evalue=1e-123,
Organism=Caenorhabditis elegans, GI25146553, Length=428, Percent_Identity=42.0560747663551, Blast_Score=340, Evalue=1e-93,
Organism=Caenorhabditis elegans, GI25146555, Length=428, Percent_Identity=42.0560747663551, Blast_Score=339, Evalue=1e-93,
Organism=Saccharomyces cerevisiae, GI6324109, Length=434, Percent_Identity=40.5529953917051, Blast_Score=311, Evalue=1e-85,
Organism=Drosophila melanogaster, GI21356033, Length=422, Percent_Identity=42.4170616113744, Blast_Score=331, Evalue=7e-91,

Paralogues:

None

Copy number: 940 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): PURA_RHORT (Q2RVD8)

Other databases:

- EMBL:   CP000230
- RefSeq:   YP_426194.1
- ProteinModelPortal:   Q2RVD8
- SMR:   Q2RVD8
- STRING:   Q2RVD8
- GeneID:   3833413
- GenomeReviews:   CP000230_GR
- KEGG:   rru:Rru_A1106
- NMPDR:   fig|1085.1.peg.3265
- eggNOG:   COG0104
- HOGENOM:   HBG658237
- OMA:   DYVVRYQ
- PhylomeDB:   Q2RVD8
- ProtClustDB:   PRK01117
- BioCyc:   RRUB269796:RRU_A1106-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00011
- InterPro:   IPR018220
- InterPro:   IPR001114
- PANTHER:   PTHR11846
- SMART:   SM00788
- TIGRFAMs:   TIGR00184

Pfam domain/function: PF00709 Adenylsucc_synt

EC number: =6.3.4.4

Molecular weight: Translated: 46193; Mature: 46062

Theoretical pI: Translated: 5.97; Mature: 5.97

Prosite motif: PS01266 ADENYLOSUCCIN_SYN_1; PS00513 ADENYLOSUCCIN_SYN_2

Important sites: ACT_SITE 13-13 ACT_SITE 41-41 BINDING 129-129 BINDING 143-143 BINDING 223-223 BINDING 238-238 BINDING 302-302 BINDING 304-304

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
0.7 %Met     (Translated Protein)
1.4 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
0.5 %Met     (Mature Protein)
1.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSNVAVVGSQWGDEGKGKVVDWLAERADVVVRFQGGHNAGHTLVVDGVTYKLSLLPSGAV
CCCEEEEECCCCCCCCCHHHHHHHHCCCEEEEEECCCCCCCEEEEECCEEEEEECCCCCC
RGKLSIIGNGVVVDPWALFEEIERVAAQGVVITPEGLRIAENAVLILPLHGNLDRAREAA
CEEEEEEECCEEECHHHHHHHHHHHHHCCEEECCCCCEEECCEEEEEEECCCHHHHHHHC
AGNAKIGTTGRGIGPAYEDKVARRAIRLCDLADEDVLRFKVERLLTHHNALLRGLGEPEL
CCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCC
DADSLISALLEIAPRILPFASPVWKVLDEARRAGKKILFEGAQGAMLDVDHGTYPYVTSS
CHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCEEEEECCCCCEEECCCCCCCEEECC
NTVAPNAATGAGVGPSSVGFTLGITKAYTTRVGAGPFPTELFDDIGRTIGERGREFGTVT
CCCCCCCCCCCCCCCCCCCEEEEEHHHHHHCCCCCCCCHHHHHHHHHHHHHCCCEECCCC
GRPRRCGWFDAVLVRQSVLVGGLRGIALTKLDVLDGLDELKICTGYRLDGQVIDHLPASM
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHCCCCCCCHHHHCCCCHH
KAQSRVEPIYETLEGWKDSTFGARSWADLPAKAIKYIRRLEELIEAPVALLSTSPEREDT
HHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCE
ILVRDPFLD
EEEECCCCC
>Mature Secondary Structure 
SNVAVVGSQWGDEGKGKVVDWLAERADVVVRFQGGHNAGHTLVVDGVTYKLSLLPSGAV
CCEEEEECCCCCCCCCHHHHHHHHCCCEEEEEECCCCCCCEEEEECCEEEEEECCCCCC
RGKLSIIGNGVVVDPWALFEEIERVAAQGVVITPEGLRIAENAVLILPLHGNLDRAREAA
CEEEEEEECCEEECHHHHHHHHHHHHHCCEEECCCCCEEECCEEEEEEECCCHHHHHHHC
AGNAKIGTTGRGIGPAYEDKVARRAIRLCDLADEDVLRFKVERLLTHHNALLRGLGEPEL
CCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCC
DADSLISALLEIAPRILPFASPVWKVLDEARRAGKKILFEGAQGAMLDVDHGTYPYVTSS
CHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCEEEEECCCCCEEECCCCCCCEEECC
NTVAPNAATGAGVGPSSVGFTLGITKAYTTRVGAGPFPTELFDDIGRTIGERGREFGTVT
CCCCCCCCCCCCCCCCCCCEEEEEHHHHHHCCCCCCCCHHHHHHHHHHHHHCCCEECCCC
GRPRRCGWFDAVLVRQSVLVGGLRGIALTKLDVLDGLDELKICTGYRLDGQVIDHLPASM
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHCCCCCCCHHHHCCCCHH
KAQSRVEPIYETLEGWKDSTFGARSWADLPAKAIKYIRRLEELIEAPVALLSTSPEREDT
HHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCE
ILVRDPFLD
EEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA