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The map label for this gene is hisZ

Identifier: 83592441

GI number: 83592441

Start: 1297869

End: 1299023

Strand: Direct

Name: hisZ

Synonym: Rru_A1105

Alternate gene names: 83592441

Gene position: 1297869-1299023 (Clockwise)

Preceding gene: 83592440

Following gene: 83592442

Centisome position: 29.82

GC content: 69.52

Gene sequence:

>1155_bases
ATGACCGTGACCGCCAAGACCGCCGCCAGGGCGCTTCTGCCCAACGGCCTGCGAGATGTCCTGCCCCCCGACGCCGCTTT
CGAAGTGGCGACGGTGGAGCGTCTGGTATCCGTTTTCGCCGCCAATGGCTATGAGCGGGTCAAGACGCCGTTGATCGAAT
TCGAGGAGGGGCTGCTGACCGGCGCCAGTCGGGCCACGGCCGGCCAGACCTTCCGCGTCATGGATCCGATCACCCAACGC
ATGATGGGGCTGCGCGCCGATATCACCATCCAGGTCGCCCGTCTGGCGGCCTCGCGGCTGGCCAAGGCGCCCCGGCCGTT
GCGCCTGACCTATTCGGGGCAGGTTCTGCGGGTCAAGGGTACCCAGCTTCGCCCCGAACGCCAGTTCACCCAGGCCGGAA
TCGAGCTGATCGGCGCCGGCGGCGTCGATGCTATCGCCGAATCCGTGCTGCTGACGGTCGAGGCCCTGGCCCAGGCCGGT
TTGCCGCGGGTCACCGTCGATCTGAACTGCCCGCCGCTGGTGCCGGCTTTGTGCCGGGCGCTCGGGCTGTCGGAGGATCT
GGCAGGCGAGTTGCGCAAGCTGCTTGAACAAAAGGATTTCACCGCCGCCGCCGCCCTGGCCGGTGACGCCGCCGGGGCGC
TGTCGGCGCTTTCGGGAACGGTCGGACCGGCCGAGCGCGCCCTGCCGGTGCTGGCCGCCCTTGATTTGCCCGGGGAGGCG
GCCGGGCATCGCGACGCCCTGTTGGCGCTGGCGCGCAAGGTGATGACCGCCGCGCCCGAGGTCGATGTCACCGTCGATCC
GCTCGAACACAAGGGCTTCGAATATTATACCGGATGCAGTTTCGCCCTGTTCGCCCAGGGGGCGCGCGGCGAGCTTGGCC
GGGGTGGCGAATATCCCGGCGATCTGAACGGGACGGTTGAACCCTGCTGCGGCGCCACCTTGTATATGGACGCGGTGCTT
GATGGCATGGTCCGCCCGGCGCCGGCGCCGCGCGTTCTGGTGGCCTCGACCCTGGCGAGGACGACACGGCGCGCCTTGCA
GGCCGATGGCTGGGTGACCGTGCCCGCCCTGACTGCCGAAGCCGGCGCGGCCGAGGCCCGTCGTCTGGGCTGTGGCCATG
TTTTGGACGATGAGGGACGGCCCGTCGCCGTCTGA

Upstream 100 bases:

>100_bases
CGCCCCGTCCCCAAACGTCCTTTGAGGACCGTTTCCGTTTGCGCGGCCGCCCCGTTCCGCGCTAAGGAAAAGGCCCATAG
GCTTCCCCGATGATCGCCCG

Downstream 100 bases:

>100_bases
GCCGGAAGCAAGGACCCCGGGCAGGGGCCGATCGTCGTGGGTGATCCACGGCCGGCCGCCCGGGATTTCCCATGAATTTT
TCTCGAATACGGGATGGATG

Product: tRNA synthetase, class II (G, H, P and S)

Products: AMP; diphosphate; L-histidyl-tRNA(His)

Alternate protein names: NA

Number of amino acids: Translated: 384; Mature: 383

Protein sequence:

>384_residues
MTVTAKTAARALLPNGLRDVLPPDAAFEVATVERLVSVFAANGYERVKTPLIEFEEGLLTGASRATAGQTFRVMDPITQR
MMGLRADITIQVARLAASRLAKAPRPLRLTYSGQVLRVKGTQLRPERQFTQAGIELIGAGGVDAIAESVLLTVEALAQAG
LPRVTVDLNCPPLVPALCRALGLSEDLAGELRKLLEQKDFTAAAALAGDAAGALSALSGTVGPAERALPVLAALDLPGEA
AGHRDALLALARKVMTAAPEVDVTVDPLEHKGFEYYTGCSFALFAQGARGELGRGGEYPGDLNGTVEPCCGATLYMDAVL
DGMVRPAPAPRVLVASTLARTTRRALQADGWVTVPALTAEAGAAEARRLGCGHVLDDEGRPVAV

Sequences:

>Translated_384_residues
MTVTAKTAARALLPNGLRDVLPPDAAFEVATVERLVSVFAANGYERVKTPLIEFEEGLLTGASRATAGQTFRVMDPITQR
MMGLRADITIQVARLAASRLAKAPRPLRLTYSGQVLRVKGTQLRPERQFTQAGIELIGAGGVDAIAESVLLTVEALAQAG
LPRVTVDLNCPPLVPALCRALGLSEDLAGELRKLLEQKDFTAAAALAGDAAGALSALSGTVGPAERALPVLAALDLPGEA
AGHRDALLALARKVMTAAPEVDVTVDPLEHKGFEYYTGCSFALFAQGARGELGRGGEYPGDLNGTVEPCCGATLYMDAVL
DGMVRPAPAPRVLVASTLARTTRRALQADGWVTVPALTAEAGAAEARRLGCGHVLDDEGRPVAV
>Mature_383_residues
TVTAKTAARALLPNGLRDVLPPDAAFEVATVERLVSVFAANGYERVKTPLIEFEEGLLTGASRATAGQTFRVMDPITQRM
MGLRADITIQVARLAASRLAKAPRPLRLTYSGQVLRVKGTQLRPERQFTQAGIELIGAGGVDAIAESVLLTVEALAQAGL
PRVTVDLNCPPLVPALCRALGLSEDLAGELRKLLEQKDFTAAAALAGDAAGALSALSGTVGPAERALPVLAALDLPGEAA
GHRDALLALARKVMTAAPEVDVTVDPLEHKGFEYYTGCSFALFAQGARGELGRGGEYPGDLNGTVEPCCGATLYMDAVLD
GMVRPAPAPRVLVASTLARTTRRALQADGWVTVPALTAEAGAAEARRLGCGHVLDDEGRPVAV

Specific function: Required for the first step of histidine biosynthesis. May allow the feedback regulation of ATP phosphoribosyltransferase activity by histidine

COG id: COG3705

COG function: function code E; ATP phosphoribosyltransferase involved in histidine biosynthesis

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family. HisZ subfamily

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): HISZ_RHORT (Q2RVD9)

Other databases:

- EMBL:   CP000230
- RefSeq:   YP_426193.1
- ProteinModelPortal:   Q2RVD9
- STRING:   Q2RVD9
- GeneID:   3833412
- GenomeReviews:   CP000230_GR
- KEGG:   rru:Rru_A1105
- NMPDR:   fig|1085.1.peg.3266
- eggNOG:   COG3705
- HOGENOM:   HBG616575
- OMA:   LTHIDAG
- ProtClustDB:   CLSK933083
- BioCyc:   RRUB269796:RRU_A1105-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00125
- InterPro:   IPR006195
- InterPro:   IPR004516
- PANTHER:   PTHR11476
- PIRSF:   PIRSF001549

Pfam domain/function: NA

EC number: 6.1.1.21

Molecular weight: Translated: 40044; Mature: 39912

Theoretical pI: Translated: 5.53; Mature: 5.53

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTVTAKTAARALLPNGLRDVLPPDAAFEVATVERLVSVFAANGYERVKTPLIEFEEGLLT
CCCCHHHHHHHHCCCCHHHHCCCCCHHHHHHHHHHHHHHHCCCHHHHHCHHHHHHHHHHC
GASRATAGQTFRVMDPITQRMMGLRADITIQVARLAASRLAKAPRPLRLTYSGQVLRVKG
CCCCCCCCCEEEHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCEEEEECCEEEEEEC
TQLRPERQFTQAGIELIGAGGVDAIAESVLLTVEALAQAGLPRVTVDLNCPPLVPALCRA
CCCCCHHHHHHHCHHEECCCCHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHH
LGLSEDLAGELRKLLEQKDFTAAAALAGDAAGALSALSGTVGPAERALPVLAALDLPGEA
HCCCHHHHHHHHHHHHHCCCHHHHHHHCCHHHHHHHHCCCCCCHHHHHHHHHEECCCCCC
AGHRDALLALARKVMTAAPEVDVTVDPLEHKGFEYYTGCSFALFAQGARGELGRGGEYPG
CCHHHHHHHHHHHHHHCCCCCCEEECCHHCCCCEEECCCCEEEEECCCCCCCCCCCCCCC
DLNGTVEPCCGATLYMDAVLDGMVRPAPAPRVLVASTLARTTRRALQADGWVTVPALTAE
CCCCCCCCCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCCEEECCCCCC
AGAAEARRLGCGHVLDDEGRPVAV
CCHHHHHHCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
TVTAKTAARALLPNGLRDVLPPDAAFEVATVERLVSVFAANGYERVKTPLIEFEEGLLT
CCCHHHHHHHHCCCCHHHHCCCCCHHHHHHHHHHHHHHHCCCHHHHHCHHHHHHHHHHC
GASRATAGQTFRVMDPITQRMMGLRADITIQVARLAASRLAKAPRPLRLTYSGQVLRVKG
CCCCCCCCCEEEHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCEEEEECCEEEEEEC
TQLRPERQFTQAGIELIGAGGVDAIAESVLLTVEALAQAGLPRVTVDLNCPPLVPALCRA
CCCCCHHHHHHHCHHEECCCCHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHH
LGLSEDLAGELRKLLEQKDFTAAAALAGDAAGALSALSGTVGPAERALPVLAALDLPGEA
HCCCHHHHHHHHHHHHHCCCHHHHHHHCCHHHHHHHHCCCCCCHHHHHHHHHEECCCCCC
AGHRDALLALARKVMTAAPEVDVTVDPLEHKGFEYYTGCSFALFAQGARGELGRGGEYPG
CCHHHHHHHHHHHHHHCCCCCCEEECCHHCCCCEEECCCCEEEEECCCCCCCCCCCCCCC
DLNGTVEPCCGATLYMDAVLDGMVRPAPAPRVLVASTLARTTRRALQADGWVTVPALTAE
CCCCCCCCCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCCEEECCCCCC
AGAAEARRLGCGHVLDDEGRPVAV
CCHHHHHHCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: Mg2+ [C]

Kcat value (1/min): 8520 [C]

Specific activity: 121.8

Km value (mM): 0.06 {His}} 0.046 {His}} 0.03 {His}} 0.02 {His}} 0.017 {His}} 0.011 {His}} 0.008 {His}} 0.0014 {tRNAHis}} 0.00056 {tRNAHis}} 0.0016 {U73tRNAHisGUG}} 0.00031 {U73tRNAHisGUG}} 0.89 {ATP}} 0.74 {ATP}} 0.655 {ATP}} 0.5

Substrates: ATP; L-histidine; tRNA(His)

Specific reaction: ATP + L-histidine + tRNA(His) = AMP + diphosphate + L-histidyl-tRNA(His)

General reaction: Aminoacylation; Esterification [C]

Inhibitor: 1, 2, 4-Triazole-3-alanine; D-Histidine; Diphosphate; L-Histidinol; Mg2+; N-Acetyl histidine; NEM; p-Chloromercuribenzoate [C]

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA