Click here to switch to the map view.

The map label for this gene is tolB

Identifier: 83592430

GI number: 83592430

Start: 1282972

End: 1284303

Strand: Direct

Name: tolB

Synonym: Rru_A1094

Alternate gene names: 83592430

Gene position: 1282972-1284303 (Clockwise)

Preceding gene: 83592429

Following gene: 83592431

Centisome position: 29.47

GC content: 62.01

Gene sequence:

>1332_bases
ATGACGCGATTGGCGAAAGGCAAATGGCGCTCGACTCTGGGCGCCATGATGGCATTGGCGGTTATGGTCGCGGCCATTCC
ACAGGCCAGGGCCGAACTGGTGATCGACATCACGCGCGGCGTGCGCGAACCGATGCCCATCGCCATTCCGGTTTTCGGTG
GGACGGACGCCCAGTCCAGCGCCATGGGGCGCGATGTGGTGGGCGTGGTGTCCAATGATCTGCAGGGATCGGGATTGTTC
CGGGTGCTCGATCCGGCCGCCTATATCCAATCCCTGCCCAATCTGGCGGTGCAGCCCCAGTTCGCCGACTGGCGGGCGAT
CAACGCCCAGGCCCTGGTTCAGGGCGAGGTTCAGCCCCAGGGCGATGGCCGTCTGCGCGTGGCCTTCCGCTTGTGGGACG
TGTTCAGCGGCCAGCAGGTCGTCGGCCGCGCCTTCTTGACCCAGGGCGATAACTGGCGCCGGGTGTCCCATATCATCGCC
GACGAGATCTACAAGGCGATCACCGGGGAAGAGGGCTATTTCGATACCCGCGTCGTCTATGTCGCCGAGACCGGCCCCAA
GACCAACCGGGTCAAGAAGCTGGCGATCATGGATCAGGACGGCGCCAATCAGCGCAATCTGACCAATGGCGAGTCCATGG
TGCTGACCCCGCGCTTCAGCCCGACCGCCCAGGAAATCACCTACCTCAGCTATTACAACAGCGTCCCCCGGGTCTATCTG
TTCAACATCGAGACCGGCCGCCGCGAATTGCTGGGCGATTTCCCCGGCATGACCTTCGCCCCGCGCTTCTCGCCCGATGG
CAATAAGGTCATCATGTCGATGGCCCTCGACGGCAATACCGAAATCTACGAGATGGATCTGCGCACCCGGCGCTCGAGCC
GGCTGACCAACCATCCGTCGATCGATACCTCGCCGTCCTATGCGCCCGATGGCCGGCAGATCACTTTCAACTCCGATCGC
GGCGGCGCCCAGCAGATCTATGTGATGAATGCCGACGGATCGGGGGTGCAAAGGATCAGTTTCGGCGATGGCCGCTATGC
AACGCCGGTATGGTCGCCACGCGGTGACCTCATTGCCTTTACCAAGCTGAAGGGCGGAAGGTTCTTTATTGGGGCCATGC
GGCCCGATGGAAGCGGCGAAAAGATCCTGACCGAGGGATTTCTGGTTGAAGGTCCGACCTGGGCACCCAACGGCAGGGTG
TTGATGTTCTTTCGTCAAGACCCCAATGGCAGAACGACGCTACACAGCATCGACGTAACCGGTTATAATGAGCGCCAGAT
TTCCACGCCGACGGAGGCCTCTGACCCAGCATGGTCGCCCCTTCTGCCATGA

Upstream 100 bases:

>100_bases
TTATGGACCGGTCGACGCGAGAGAGAAACGAGGGCGCGCGCTCCCCTGGCGCGCGTTCATGACGGAATTCACGGCAGAAA
AAGGCGAGGGGTTTCCTCCT

Downstream 100 bases:

>100_bases
CGTAAAGGAAGGGGCCTTGTCTTCCCGTGCGATATCCCTCACAAACGGTCCTCAGAATATAAACTGCAGGTCCGGTGTGT
CTTTACGGTATGAACCCATG

Product: translocation protein TolB

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 443; Mature: 442

Protein sequence:

>443_residues
MTRLAKGKWRSTLGAMMALAVMVAAIPQARAELVIDITRGVREPMPIAIPVFGGTDAQSSAMGRDVVGVVSNDLQGSGLF
RVLDPAAYIQSLPNLAVQPQFADWRAINAQALVQGEVQPQGDGRLRVAFRLWDVFSGQQVVGRAFLTQGDNWRRVSHIIA
DEIYKAITGEEGYFDTRVVYVAETGPKTNRVKKLAIMDQDGANQRNLTNGESMVLTPRFSPTAQEITYLSYYNSVPRVYL
FNIETGRRELLGDFPGMTFAPRFSPDGNKVIMSMALDGNTEIYEMDLRTRRSSRLTNHPSIDTSPSYAPDGRQITFNSDR
GGAQQIYVMNADGSGVQRISFGDGRYATPVWSPRGDLIAFTKLKGGRFFIGAMRPDGSGEKILTEGFLVEGPTWAPNGRV
LMFFRQDPNGRTTLHSIDVTGYNERQISTPTEASDPAWSPLLP

Sequences:

>Translated_443_residues
MTRLAKGKWRSTLGAMMALAVMVAAIPQARAELVIDITRGVREPMPIAIPVFGGTDAQSSAMGRDVVGVVSNDLQGSGLF
RVLDPAAYIQSLPNLAVQPQFADWRAINAQALVQGEVQPQGDGRLRVAFRLWDVFSGQQVVGRAFLTQGDNWRRVSHIIA
DEIYKAITGEEGYFDTRVVYVAETGPKTNRVKKLAIMDQDGANQRNLTNGESMVLTPRFSPTAQEITYLSYYNSVPRVYL
FNIETGRRELLGDFPGMTFAPRFSPDGNKVIMSMALDGNTEIYEMDLRTRRSSRLTNHPSIDTSPSYAPDGRQITFNSDR
GGAQQIYVMNADGSGVQRISFGDGRYATPVWSPRGDLIAFTKLKGGRFFIGAMRPDGSGEKILTEGFLVEGPTWAPNGRV
LMFFRQDPNGRTTLHSIDVTGYNERQISTPTEASDPAWSPLLP
>Mature_442_residues
TRLAKGKWRSTLGAMMALAVMVAAIPQARAELVIDITRGVREPMPIAIPVFGGTDAQSSAMGRDVVGVVSNDLQGSGLFR
VLDPAAYIQSLPNLAVQPQFADWRAINAQALVQGEVQPQGDGRLRVAFRLWDVFSGQQVVGRAFLTQGDNWRRVSHIIAD
EIYKAITGEEGYFDTRVVYVAETGPKTNRVKKLAIMDQDGANQRNLTNGESMVLTPRFSPTAQEITYLSYYNSVPRVYLF
NIETGRRELLGDFPGMTFAPRFSPDGNKVIMSMALDGNTEIYEMDLRTRRSSRLTNHPSIDTSPSYAPDGRQITFNSDRG
GAQQIYVMNADGSGVQRISFGDGRYATPVWSPRGDLIAFTKLKGGRFFIGAMRPDGSGEKILTEGFLVEGPTWAPNGRVL
MFFRQDPNGRTTLHSIDVTGYNERQISTPTEASDPAWSPLLP

Specific function: Involved in the tonB-independent uptake of proteins

COG id: COG0823

COG function: function code U; Periplasmic component of the Tol biopolymer transport system

Gene ontology:

Cell location: Periplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tolB family

Homologues:

Organism=Escherichia coli, GI1786961, Length=436, Percent_Identity=28.6697247706422, Blast_Score=176, Evalue=2e-45,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TOLB_RHORT (Q2RVF0)

Other databases:

- EMBL:   CP000230
- RefSeq:   YP_426182.1
- ProteinModelPortal:   Q2RVF0
- SMR:   Q2RVF0
- STRING:   Q2RVF0
- GeneID:   3833401
- GenomeReviews:   CP000230_GR
- KEGG:   rru:Rru_A1094
- NMPDR:   fig|1085.1.peg.3277
- eggNOG:   COG0823
- HOGENOM:   HBG644919
- OMA:   RVADYDG
- PhylomeDB:   Q2RVF0
- ProtClustDB:   PRK05137
- BioCyc:   RRUB269796:RRU_A1094-MONOMER
- HAMAP:   MF_00671
- InterPro:   IPR011659
- InterPro:   IPR014167
- InterPro:   IPR007195
- InterPro:   IPR015943
- Gene3D:   G3DSA:3.40.50.10070
- Gene3D:   G3DSA:2.130.10.10
- TIGRFAMs:   TIGR02800

Pfam domain/function: PF07676 PD40; PF04052 TolB_N; SSF52964 TolB_N

EC number: NA

Molecular weight: Translated: 48813; Mature: 48682

Theoretical pI: Translated: 9.06; Mature: 9.06

Prosite motif: PS00639 THIOL_PROTEASE_HIS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTRLAKGKWRSTLGAMMALAVMVAAIPQARAELVIDITRGVREPMPIAIPVFGGTDAQSS
CCCCCCCHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEHHCCCCCCCEEEEEECCCCCCCH
AMGRDVVGVVSNDLQGSGLFRVLDPAAYIQSLPNLAVQPQFADWRAINAQALVQGEVQPQ
HCCCCEEEEECCCCCCCCEEEEECHHHHHHHCCCCEECCCCCCCEECCCEEEEEECCCCC
GDGRLRVAFRLWDVFSGQQVVGRAFLTQGDNWRRVSHIIADEIYKAITGEEGYFDTRVVY
CCCEEEEEEEEEECCCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCCCEEEEEEE
VAETGPKTNRVKKLAIMDQDGANQRNLTNGESMVLTPRFSPTAQEITYLSYYNSVPRVYL
EEECCCCCCCEEEEEEECCCCCCCCCCCCCCEEEEECCCCCCHHHEEEEEHHCCCCEEEE
FNIETGRRELLGDFPGMTFAPRFSPDGNKVIMSMALDGNTEIYEMDLRTRRSSRLTNHPS
EEECCCHHHHHHCCCCCEECCCCCCCCCEEEEEEEECCCCEEEEEEHHHHHHHCCCCCCC
IDTSPSYAPDGRQITFNSDRGGAQQIYVMNADGSGVQRISFGDGRYATPVWSPRGDLIAF
CCCCCCCCCCCCEEEEECCCCCCEEEEEEECCCCCEEEEECCCCCEECCCCCCCCCEEEE
TKLKGGRFFIGAMRPDGSGEKILTEGFLVEGPTWAPNGRVLMFFRQDPNGRTTLHSIDVT
EEECCCEEEEEEECCCCCCCEEEECCEEEECCCCCCCCCEEEEEEECCCCCEEEEEEEEC
GYNERQISTPTEASDPAWSPLLP
CCCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
TRLAKGKWRSTLGAMMALAVMVAAIPQARAELVIDITRGVREPMPIAIPVFGGTDAQSS
CCCCCCHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEHHCCCCCCCEEEEEECCCCCCCH
AMGRDVVGVVSNDLQGSGLFRVLDPAAYIQSLPNLAVQPQFADWRAINAQALVQGEVQPQ
HCCCCEEEEECCCCCCCCEEEEECHHHHHHHCCCCEECCCCCCCEECCCEEEEEECCCCC
GDGRLRVAFRLWDVFSGQQVVGRAFLTQGDNWRRVSHIIADEIYKAITGEEGYFDTRVVY
CCCEEEEEEEEEECCCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCCCEEEEEEE
VAETGPKTNRVKKLAIMDQDGANQRNLTNGESMVLTPRFSPTAQEITYLSYYNSVPRVYL
EEECCCCCCCEEEEEEECCCCCCCCCCCCCCEEEEECCCCCCHHHEEEEEHHCCCCEEEE
FNIETGRRELLGDFPGMTFAPRFSPDGNKVIMSMALDGNTEIYEMDLRTRRSSRLTNHPS
EEECCCHHHHHHCCCCCEECCCCCCCCCEEEEEEEECCCCEEEEEEHHHHHHHCCCCCCC
IDTSPSYAPDGRQITFNSDRGGAQQIYVMNADGSGVQRISFGDGRYATPVWSPRGDLIAF
CCCCCCCCCCCCEEEEECCCCCCEEEEEEECCCCCEEEEECCCCCEECCCCCCCCCEEEE
TKLKGGRFFIGAMRPDGSGEKILTEGFLVEGPTWAPNGRVLMFFRQDPNGRTTLHSIDVT
EEECCCEEEEEEECCCCCCCEEEECCEEEECCCCCCCCCEEEEEEECCCCCEEEEEEEEC
GYNERQISTPTEASDPAWSPLLP
CCCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA