Click here to switch to the map view.

The map label for this gene is pfkA1 [H]

Identifier: 83592383

GI number: 83592383

Start: 1240770

End: 1241870

Strand: Reverse

Name: pfkA1 [H]

Synonym: Rru_A1047

Alternate gene names: 83592383

Gene position: 1241870-1240770 (Counterclockwise)

Preceding gene: 83592385

Following gene: 83592382

Centisome position: 28.53

GC content: 66.94

Gene sequence:

>1101_bases
ATGGCCGAGATTAGACGCATCGGAGTACTCACCAGCGGCGGCGATTGCGCCGGGCTCAACGCGGTGATCCGGGCGGTTAC
CCACCGCGCCCGCCGGGCCTACGGCTGGAAAGTGTTCGGTATTCTCGACGGAACCATGGGGCTGATGGACCGCCCCTTGC
GCTACCGCGAGCTCGACTCGGAAATGTTTTCGGGCTTCGACATTCTGCGCGCCGGCGGCACCGTTCTGGGTACCGTCAAC
AAGGGCGACCCCTTCGCCTTCCCCATGGCCGACGGTTCCAAGACCGACCGCTCGCTTGACTTCGTCGACGGCTTCCACAG
CCTGGAACTCGACGCCCTGGTGGTGGTCGGCGGCGACGGCTCGATGCGCATTCTCAAGAAGCTGTGCGACAAGGGCAGCA
TCGGCATGGTCGGCGTGCCCAAGACCATCGACAACGATGTTCACGGCACCGAATACGCCGTCGGCTTCTCCACCGCCACC
AATGTGGTGACCGAGGCCCTGGACCGTCTGCAGGCGACCGCTGCCAGTCACCACCGGGTGATGATCCTGGAAGTCATGGG
CCGCGACGCCGGCCATATCGCCGTCAGCGCCGGCATCGCCGGCGGCGCCGATGTCATCTTGATCCCCGAGATCCCCTATA
CCCTGGAAGGCGTGGCCAAGCGCATCCGCGAGGTTCAGCAGGAAGGCCGCAGCCACGCCCTGATCGTCGTCGCCGAGGGC
GTGCTGACCCCCGAGGGCGAGCGCGCCACCGTGGCCTATGCCGGTGGCCAAACCCGCTATGGCGGCATCAGCCAGTATCT
GTCCGACCGCATCGCCCAGGACACCGGCACCGAAACCCGGGTGACCATCCTCGGCCACGTCCAGCGCGGCGGCATTCCTT
CGATGCGCGACCGCCTCCTCGCCTCGGCCTTCGGCGTCCATGCCGTCGATCTGGTGGCGGCGCGCAAATTCGGCCGCATG
GTGGCTTGGCAGGACCGGGGCGTCGTCGACGTGCCGCTGGAAGAGGTCTGCATCGGACCGCGCAGCCTTGATCCCAATGG
TACCCTGGTCCATGCCGCCCGCGGCCTGGGCATCTATGTCGGCGAAATCGGCGCCGCGTAA

Upstream 100 bases:

>100_bases
CTGGCGTGTTATACCTTGAGTCCAAAGCCGAATTGACCCACCGTGGTTGCGAGCAACCACCCAGAACAAATAACATCATA
TCCGATCAAGGGAACATGTC

Downstream 100 bases:

>100_bases
TATTCTTTCGTTTAGCCGGGTTTTGCTTTTAAAGCCAGCCCGAGTCGTTGGGGCGACCGCCGAAAAACTCGGCGGTCGCC
TTTGTTTTCGGCCAATAACC

Product: 6-phosphofructokinase

Products: NA

Alternate protein names: Phosphofructokinase 1; Phosphohexokinase 1 [H]

Number of amino acids: Translated: 366; Mature: 365

Protein sequence:

>366_residues
MAEIRRIGVLTSGGDCAGLNAVIRAVTHRARRAYGWKVFGILDGTMGLMDRPLRYRELDSEMFSGFDILRAGGTVLGTVN
KGDPFAFPMADGSKTDRSLDFVDGFHSLELDALVVVGGDGSMRILKKLCDKGSIGMVGVPKTIDNDVHGTEYAVGFSTAT
NVVTEALDRLQATAASHHRVMILEVMGRDAGHIAVSAGIAGGADVILIPEIPYTLEGVAKRIREVQQEGRSHALIVVAEG
VLTPEGERATVAYAGGQTRYGGISQYLSDRIAQDTGTETRVTILGHVQRGGIPSMRDRLLASAFGVHAVDLVAARKFGRM
VAWQDRGVVDVPLEEVCIGPRSLDPNGTLVHAARGLGIYVGEIGAA

Sequences:

>Translated_366_residues
MAEIRRIGVLTSGGDCAGLNAVIRAVTHRARRAYGWKVFGILDGTMGLMDRPLRYRELDSEMFSGFDILRAGGTVLGTVN
KGDPFAFPMADGSKTDRSLDFVDGFHSLELDALVVVGGDGSMRILKKLCDKGSIGMVGVPKTIDNDVHGTEYAVGFSTAT
NVVTEALDRLQATAASHHRVMILEVMGRDAGHIAVSAGIAGGADVILIPEIPYTLEGVAKRIREVQQEGRSHALIVVAEG
VLTPEGERATVAYAGGQTRYGGISQYLSDRIAQDTGTETRVTILGHVQRGGIPSMRDRLLASAFGVHAVDLVAARKFGRM
VAWQDRGVVDVPLEEVCIGPRSLDPNGTLVHAARGLGIYVGEIGAA
>Mature_365_residues
AEIRRIGVLTSGGDCAGLNAVIRAVTHRARRAYGWKVFGILDGTMGLMDRPLRYRELDSEMFSGFDILRAGGTVLGTVNK
GDPFAFPMADGSKTDRSLDFVDGFHSLELDALVVVGGDGSMRILKKLCDKGSIGMVGVPKTIDNDVHGTEYAVGFSTATN
VVTEALDRLQATAASHHRVMILEVMGRDAGHIAVSAGIAGGADVILIPEIPYTLEGVAKRIREVQQEGRSHALIVVAEGV
LTPEGERATVAYAGGQTRYGGISQYLSDRIAQDTGTETRVTILGHVQRGGIPSMRDRLLASAFGVHAVDLVAARKFGRMV
AWQDRGVVDVPLEEVCIGPRSLDPNGTLVHAARGLGIYVGEIGAA

Specific function: Key control step of glycolysis. [C]

COG id: COG0205

COG function: function code G; 6-phosphofructokinase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the phosphofructokinase family [H]

Homologues:

Organism=Homo sapiens, GI48762920, Length=330, Percent_Identity=34.2424242424242, Blast_Score=156, Evalue=3e-38,
Organism=Homo sapiens, GI11321601, Length=330, Percent_Identity=34.5454545454545, Blast_Score=153, Evalue=3e-37,
Organism=Homo sapiens, GI266453768, Length=330, Percent_Identity=34.2424242424242, Blast_Score=147, Evalue=2e-35,
Organism=Homo sapiens, GI266453748, Length=330, Percent_Identity=34.2424242424242, Blast_Score=147, Evalue=2e-35,
Organism=Homo sapiens, GI4505749, Length=330, Percent_Identity=34.2424242424242, Blast_Score=147, Evalue=2e-35,
Organism=Homo sapiens, GI266453619, Length=330, Percent_Identity=34.2424242424242, Blast_Score=147, Evalue=2e-35,
Organism=Escherichia coli, GI1790350, Length=328, Percent_Identity=38.109756097561, Blast_Score=197, Evalue=9e-52,
Organism=Caenorhabditis elegans, GI17558788, Length=332, Percent_Identity=31.0240963855422, Blast_Score=136, Evalue=2e-32,
Organism=Caenorhabditis elegans, GI25147584, Length=330, Percent_Identity=30.3030303030303, Blast_Score=134, Evalue=6e-32,
Organism=Caenorhabditis elegans, GI72003601, Length=159, Percent_Identity=34.5911949685535, Blast_Score=95, Evalue=7e-20,
Organism=Saccharomyces cerevisiae, GI6321679, Length=337, Percent_Identity=34.4213649851632, Blast_Score=142, Evalue=1e-34,
Organism=Saccharomyces cerevisiae, GI6323861, Length=367, Percent_Identity=31.0626702997275, Blast_Score=131, Evalue=2e-31,
Organism=Drosophila melanogaster, GI28573326, Length=327, Percent_Identity=32.4159021406728, Blast_Score=142, Evalue=4e-34,
Organism=Drosophila melanogaster, GI17647809, Length=328, Percent_Identity=32.6219512195122, Blast_Score=141, Evalue=6e-34,
Organism=Drosophila melanogaster, GI24652337, Length=328, Percent_Identity=32.6219512195122, Blast_Score=141, Evalue=7e-34,

Paralogues:

None

Copy number: 3981 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 950 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012003
- InterPro:   IPR022953
- InterPro:   IPR015912
- InterPro:   IPR000023 [H]

Pfam domain/function: PF00365 PFK [H]

EC number: =2.7.1.11 [H]

Molecular weight: Translated: 38861; Mature: 38730

Theoretical pI: Translated: 6.68; Mature: 6.68

Prosite motif: PS00433 PHOSPHOFRUCTOKINASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAEIRRIGVLTSGGDCAGLNAVIRAVTHRARRAYGWKVFGILDGTMGLMDRPLRYRELDS
CCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHCCEEEEEEECCCHHHHCCCCHHHHHHH
EMFSGFDILRAGGTVLGTVNKGDPFAFPMADGSKTDRSLDFVDGFHSLELDALVVVGGDG
HHHCCCHHEECCCEEEEECCCCCCEEEECCCCCCCCCCCHHHCCCCCCCEEEEEEECCCC
SMRILKKLCDKGSIGMVGVPKTIDNDVHGTEYAVGFSTATNVVTEALDRLQATAASHHRV
CHHHHHHHHCCCCCCEEECCHHCCCCCCCCEEEEEHHHHHHHHHHHHHHHHHHHCCCCEE
MILEVMGRDAGHIAVSAGIAGGADVILIPEIPYTLEGVAKRIREVQQEGRSHALIVVAEG
EEEEEECCCCCCEEEEECCCCCCCEEEECCCCHHHHHHHHHHHHHHHCCCCEEEEEEECC
VLTPEGERATVAYAGGQTRYGGISQYLSDRIAQDTGTETRVTILGHVQRGGIPSMRDRLL
EECCCCCEEEEEEECCCCCCCHHHHHHHHHHHHCCCCCEEEEEEEEECCCCCHHHHHHHH
ASAFGVHAVDLVAARKFGRMVAWQDRGVVDVPLEEVCIGPRSLDPNGTLVHAARGLGIYV
HHHHCHHHHHHHHHHHHCCEEEECCCCEEECCHHHHHCCCCCCCCCCCEEEECCCCEEEE
GEIGAA
EECCCC
>Mature Secondary Structure 
AEIRRIGVLTSGGDCAGLNAVIRAVTHRARRAYGWKVFGILDGTMGLMDRPLRYRELDS
CCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHCCEEEEEEECCCHHHHCCCCHHHHHHH
EMFSGFDILRAGGTVLGTVNKGDPFAFPMADGSKTDRSLDFVDGFHSLELDALVVVGGDG
HHHCCCHHEECCCEEEEECCCCCCEEEECCCCCCCCCCCHHHCCCCCCCEEEEEEECCCC
SMRILKKLCDKGSIGMVGVPKTIDNDVHGTEYAVGFSTATNVVTEALDRLQATAASHHRV
CHHHHHHHHCCCCCCEEECCHHCCCCCCCCEEEEEHHHHHHHHHHHHHHHHHHHCCCCEE
MILEVMGRDAGHIAVSAGIAGGADVILIPEIPYTLEGVAKRIREVQQEGRSHALIVVAEG
EEEEEECCCCCCEEEEECCCCCCCEEEECCCCHHHHHHHHHHHHHHHCCCCEEEEEEECC
VLTPEGERATVAYAGGQTRYGGISQYLSDRIAQDTGTETRVTILGHVQRGGIPSMRDRLL
EECCCCCEEEEEEECCCCCCCHHHHHHHHHHHHCCCCCEEEEEEEEECCCCCHHHHHHHH
ASAFGVHAVDLVAARKFGRMVAWQDRGVVDVPLEEVCIGPRSLDPNGTLVHAARGLGIYV
HHHHCHHHHHHHHHHHHCCEEEECCCCEEECCHHHHHCCCCCCCCCCCEEEECCCCEEEE
GEIGAA
EECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11759840 [H]