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The map label for this gene is prpE [C]

Identifier: 83592382

GI number: 83592382

Start: 1238015

End: 1239919

Strand: Reverse

Name: prpE [C]

Synonym: Rru_A1046

Alternate gene names: 83592382

Gene position: 1239919-1238015 (Counterclockwise)

Preceding gene: 83592383

Following gene: 83592379

Centisome position: 28.49

GC content: 65.56

Gene sequence:

>1905_bases
ATGACCAATGCCTACGAAGAGGCCTACCAGCGGTCCATCACCGACAAGGAAGGCTTCTGGGGAGACGCCGCGCGCGATAT
CGTGTGGACCAAACCCTGGGATCGCGTGCTCGACGAGTCCCAGGCCCCCCTGTACCGCTGGTTCGCCGGCGGCGAGCTCA
ACACTTGTTATAACGCCGTCGACCGTCATGTCGACGAGGGTCGGGGCGATCAGGCGGCGATCATCTATGACAGCGCCATC
ACCGGCGCCAAACGCACGATCAGCTATAAAACCCTGAAGGATCAGGTGGCGGGCTTCGCCGGCGTGCTGGCCAGCCGGGG
TGTCGGCAAGGGCGACCGGGTCATCCTTTATATGCCGATGATCCCCGAAAGCCTTGTCGCCATGTTGGCCTGCGCCCGCC
TGGGCGCCATCCATTCCGTGGTGTTCGGCGGCTTCGCGCCCAATGAGCTGGCAACCCGCATCAACGACGCCCAGCCCAAG
GCCATCGTCGCCGCCTCCTGCGGCATCGAACCCAACCGGGTGATCGCCTATAAGCCGCTGGTCGACGAGGCGATCCGCCT
GTCCGCCCACAAGCCCGACTCGGTGATCGTCTGGCAGCGCGAGGAAGCCCGGGCCGATTTGTCCAACCCCGGCGATATTG
ATTGGGCCCTCGCCCTGGAAACCACCACGCCGCATGACTGCGTGCCGGTGGCGGCCACCGATCCGCTTTATATCCTCTAT
ACGTCGGGCACCACCGGCCAGCCCAAGGGGGTGGTGCGCGACAACGGCGGCCACGCCGTCGCCCTGCGCTGGACAATGAA
GGCGATCTACAACATGAGCCCGGGCGACGTGTTCTGGGCGGCTTCCGATGTCGGCTGGGTGGTCGGCCATTCCTATATCT
GCTACGCCCCGCTGTTGCTTGGCGCCACCACCTTGGTGTTCGAGGGCAAGCCGGTGGGCACCCCCGATGCCGGCACCTTC
TGGCGGGTGATCGCCGAGCACAAGGTGGCGACCTTGTTCACCGCCCCCACCGCCTTCCGCGCCATCAAGCGCGAGGATCC
CGACGGCCTGGAAATCGGCAAATACGACCTGTCGTCGTTGCGCGCCCTGTTCCTGGCCGGCGAGCGCTGCGATCCGGCGA
CCATCGACTGGGCCGAACACAAGCTGGGCGTTCCGGTCATCGATCATTGGTGGCAGACGGAAACCGGCTGGGCGATCGCC
GCCAATTGCCTGGGCTTGCACCTTTACCCGGTCAAGCACGGCAGCCCGACCCTGGCCGCCCCGGGCTGGGACGTGCGGGT
TCTGGATGACACCAACCACGAGGTCAATCCCGGACAGATCGGCGCCCTGGTCTGCAAACTGCCCCTGCCCCCGGGCACGC
TGACCACGCTGTGGAACGCCGAGGCGCGCTTCCGTCAAGCCTATCTCGCCGACTTCCCCGGCTATTACAAAAGCGGTGAC
GCCGGGTTCATCGACGACGAAGGCTATATCTATGTGATGACCCGCACCGACGACATCATCAATGTCGCCGGCCATCGCCT
GTCGACCGGGGCCATGGAAGAGGTGCTGGCCGGCCATCCGGCGGTGGCCGAATGCGCCGTCATCGGCATTTCCGACGATC
TCAAGGGACAGGTGCCGCTGGGCTTCGTCTGCCTGAAGGCCGGCATCACCACCGAACCGGCGCAGATCGCCAAGGAATGC
GCCGCCCTGGTCCGCGACCAGATCGGCCCGGTGGCGGCCTATAAGCAGACCGTGGTCGTGCCGCGCCTGCCCAAGACGCG
CTCGGGCAAGATCCTGCGCGGCACCATGCAAAAGATCGCCGACAACGAGGCCTTCAAGATGCCCGCGACCATCGACGATC
CGGGCATCCTGCCCGAAATCGAAGAGGCCCTGGAAAGCATCGGCTTGGCGCGCAAGCGCGTCTGA

Upstream 100 bases:

>100_bases
CCGCGTGCCAAGGGGGCTCTTAAGCGCTCTGCGGACGCGGGGTCCCGGCGCTTCGGCGAAGCGCCTGAAAAAACGTTACC
GTCGAGGGGAGAAACCAACC

Downstream 100 bases:

>100_bases
TCCGTCCTTAAACCTCGGGGGAGGCTGTGAAGCGGCCTCCCCCGGGATCGTCCAAGGCCAAAAGCCTCAGCGCATGCGCA
TCGAGCGCATCAGGCCGAAG

Product: AMP-dependent synthetase and ligase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 634; Mature: 633

Protein sequence:

>634_residues
MTNAYEEAYQRSITDKEGFWGDAARDIVWTKPWDRVLDESQAPLYRWFAGGELNTCYNAVDRHVDEGRGDQAAIIYDSAI
TGAKRTISYKTLKDQVAGFAGVLASRGVGKGDRVILYMPMIPESLVAMLACARLGAIHSVVFGGFAPNELATRINDAQPK
AIVAASCGIEPNRVIAYKPLVDEAIRLSAHKPDSVIVWQREEARADLSNPGDIDWALALETTTPHDCVPVAATDPLYILY
TSGTTGQPKGVVRDNGGHAVALRWTMKAIYNMSPGDVFWAASDVGWVVGHSYICYAPLLLGATTLVFEGKPVGTPDAGTF
WRVIAEHKVATLFTAPTAFRAIKREDPDGLEIGKYDLSSLRALFLAGERCDPATIDWAEHKLGVPVIDHWWQTETGWAIA
ANCLGLHLYPVKHGSPTLAAPGWDVRVLDDTNHEVNPGQIGALVCKLPLPPGTLTTLWNAEARFRQAYLADFPGYYKSGD
AGFIDDEGYIYVMTRTDDIINVAGHRLSTGAMEEVLAGHPAVAECAVIGISDDLKGQVPLGFVCLKAGITTEPAQIAKEC
AALVRDQIGPVAAYKQTVVVPRLPKTRSGKILRGTMQKIADNEAFKMPATIDDPGILPEIEEALESIGLARKRV

Sequences:

>Translated_634_residues
MTNAYEEAYQRSITDKEGFWGDAARDIVWTKPWDRVLDESQAPLYRWFAGGELNTCYNAVDRHVDEGRGDQAAIIYDSAI
TGAKRTISYKTLKDQVAGFAGVLASRGVGKGDRVILYMPMIPESLVAMLACARLGAIHSVVFGGFAPNELATRINDAQPK
AIVAASCGIEPNRVIAYKPLVDEAIRLSAHKPDSVIVWQREEARADLSNPGDIDWALALETTTPHDCVPVAATDPLYILY
TSGTTGQPKGVVRDNGGHAVALRWTMKAIYNMSPGDVFWAASDVGWVVGHSYICYAPLLLGATTLVFEGKPVGTPDAGTF
WRVIAEHKVATLFTAPTAFRAIKREDPDGLEIGKYDLSSLRALFLAGERCDPATIDWAEHKLGVPVIDHWWQTETGWAIA
ANCLGLHLYPVKHGSPTLAAPGWDVRVLDDTNHEVNPGQIGALVCKLPLPPGTLTTLWNAEARFRQAYLADFPGYYKSGD
AGFIDDEGYIYVMTRTDDIINVAGHRLSTGAMEEVLAGHPAVAECAVIGISDDLKGQVPLGFVCLKAGITTEPAQIAKEC
AALVRDQIGPVAAYKQTVVVPRLPKTRSGKILRGTMQKIADNEAFKMPATIDDPGILPEIEEALESIGLARKRV
>Mature_633_residues
TNAYEEAYQRSITDKEGFWGDAARDIVWTKPWDRVLDESQAPLYRWFAGGELNTCYNAVDRHVDEGRGDQAAIIYDSAIT
GAKRTISYKTLKDQVAGFAGVLASRGVGKGDRVILYMPMIPESLVAMLACARLGAIHSVVFGGFAPNELATRINDAQPKA
IVAASCGIEPNRVIAYKPLVDEAIRLSAHKPDSVIVWQREEARADLSNPGDIDWALALETTTPHDCVPVAATDPLYILYT
SGTTGQPKGVVRDNGGHAVALRWTMKAIYNMSPGDVFWAASDVGWVVGHSYICYAPLLLGATTLVFEGKPVGTPDAGTFW
RVIAEHKVATLFTAPTAFRAIKREDPDGLEIGKYDLSSLRALFLAGERCDPATIDWAEHKLGVPVIDHWWQTETGWAIAA
NCLGLHLYPVKHGSPTLAAPGWDVRVLDDTNHEVNPGQIGALVCKLPLPPGTLTTLWNAEARFRQAYLADFPGYYKSGDA
GFIDDEGYIYVMTRTDDIINVAGHRLSTGAMEEVLAGHPAVAECAVIGISDDLKGQVPLGFVCLKAGITTEPAQIAKECA
ALVRDQIGPVAAYKQTVVVPRLPKTRSGKILRGTMQKIADNEAFKMPATIDDPGILPEIEEALESIGLARKRV

Specific function: Catalyzes The Synthesis Of Propionyl-CoA From Propionate And CoA. Also Converts Acetate To Acetyl-CoA But With A Lower Specific Activity (By Similarity). [C]

COG id: COG0365

COG function: function code I; Acyl-coenzyme A synthetases/AMP-(fatty) acid ligases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATP-dependent AMP-binding enzyme family [H]

Homologues:

Organism=Homo sapiens, GI13375727, Length=627, Percent_Identity=51.993620414673, Blast_Score=700, Evalue=0.0,
Organism=Homo sapiens, GI8923896, Length=655, Percent_Identity=36.030534351145, Blast_Score=389, Evalue=1e-108,
Organism=Homo sapiens, GI253970489, Length=668, Percent_Identity=35.3293413173653, Blast_Score=384, Evalue=1e-106,
Organism=Homo sapiens, GI28416953, Length=620, Percent_Identity=36.1290322580645, Blast_Score=379, Evalue=1e-105,
Organism=Homo sapiens, GI38505220, Length=521, Percent_Identity=28.7907869481766, Blast_Score=203, Evalue=5e-52,
Organism=Homo sapiens, GI42544132, Length=522, Percent_Identity=26.8199233716475, Blast_Score=184, Evalue=3e-46,
Organism=Homo sapiens, GI115511026, Length=519, Percent_Identity=26.2042389210019, Blast_Score=174, Evalue=3e-43,
Organism=Homo sapiens, GI58082049, Length=528, Percent_Identity=26.7045454545455, Blast_Score=173, Evalue=4e-43,
Organism=Homo sapiens, GI157311624, Length=528, Percent_Identity=26.3257575757576, Blast_Score=170, Evalue=4e-42,
Organism=Homo sapiens, GI157311622, Length=528, Percent_Identity=26.3257575757576, Blast_Score=170, Evalue=4e-42,
Organism=Homo sapiens, GI122937307, Length=513, Percent_Identity=26.7056530214425, Blast_Score=165, Evalue=2e-40,
Organism=Homo sapiens, GI31982927, Length=644, Percent_Identity=23.9130434782609, Blast_Score=154, Evalue=3e-37,
Organism=Homo sapiens, GI42544134, Length=383, Percent_Identity=25.5874673629243, Blast_Score=111, Evalue=2e-24,
Organism=Homo sapiens, GI195947378, Length=463, Percent_Identity=23.9740820734341, Blast_Score=100, Evalue=4e-21,
Organism=Homo sapiens, GI187761345, Length=387, Percent_Identity=24.8062015503876, Blast_Score=77, Evalue=4e-14,
Organism=Homo sapiens, GI187761343, Length=387, Percent_Identity=24.8062015503876, Blast_Score=77, Evalue=4e-14,
Organism=Escherichia coli, GI1786529, Length=631, Percent_Identity=45.1664025356577, Blast_Score=496, Evalue=1e-141,
Organism=Escherichia coli, GI1790505, Length=635, Percent_Identity=39.2125984251969, Blast_Score=431, Evalue=1e-122,
Organism=Escherichia coli, GI145693145, Length=515, Percent_Identity=26.0194174757282, Blast_Score=127, Evalue=2e-30,
Organism=Escherichia coli, GI1788107, Length=544, Percent_Identity=24.0808823529412, Blast_Score=107, Evalue=2e-24,
Organism=Escherichia coli, GI221142682, Length=540, Percent_Identity=21.4814814814815, Blast_Score=103, Evalue=2e-23,
Organism=Escherichia coli, GI1786801, Length=468, Percent_Identity=24.5726495726496, Blast_Score=99, Evalue=7e-22,
Organism=Escherichia coli, GI1786810, Length=527, Percent_Identity=21.4421252371917, Blast_Score=81, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI71983001, Length=622, Percent_Identity=34.4051446945338, Blast_Score=321, Evalue=8e-88,
Organism=Caenorhabditis elegans, GI71982997, Length=622, Percent_Identity=34.4051446945338, Blast_Score=320, Evalue=1e-87,
Organism=Caenorhabditis elegans, GI17569507, Length=634, Percent_Identity=21.608832807571, Blast_Score=125, Evalue=8e-29,
Organism=Caenorhabditis elegans, GI17557194, Length=565, Percent_Identity=23.716814159292, Blast_Score=105, Evalue=7e-23,
Organism=Caenorhabditis elegans, GI17538037, Length=569, Percent_Identity=23.1985940246046, Blast_Score=100, Evalue=4e-21,
Organism=Caenorhabditis elegans, GI17531443, Length=526, Percent_Identity=22.8136882129278, Blast_Score=92, Evalue=1e-18,
Organism=Caenorhabditis elegans, GI71985884, Length=573, Percent_Identity=23.0366492146597, Blast_Score=92, Evalue=1e-18,
Organism=Caenorhabditis elegans, GI17559526, Length=555, Percent_Identity=21.2612612612613, Blast_Score=91, Evalue=2e-18,
Organism=Caenorhabditis elegans, GI17560140, Length=532, Percent_Identity=23.3082706766917, Blast_Score=79, Evalue=1e-14,
Organism=Saccharomyces cerevisiae, GI6319264, Length=642, Percent_Identity=38.006230529595, Blast_Score=429, Evalue=1e-121,
Organism=Saccharomyces cerevisiae, GI6323182, Length=651, Percent_Identity=37.7880184331797, Blast_Score=410, Evalue=1e-115,
Organism=Saccharomyces cerevisiae, GI6319699, Length=531, Percent_Identity=22.5988700564972, Blast_Score=101, Evalue=4e-22,
Organism=Drosophila melanogaster, GI21355225, Length=635, Percent_Identity=47.0866141732283, Blast_Score=619, Evalue=1e-177,
Organism=Drosophila melanogaster, GI24667955, Length=634, Percent_Identity=36.1198738170347, Blast_Score=383, Evalue=1e-106,
Organism=Drosophila melanogaster, GI62472339, Length=540, Percent_Identity=37.5925925925926, Blast_Score=341, Evalue=7e-94,
Organism=Drosophila melanogaster, GI24667959, Length=507, Percent_Identity=36.8836291913215, Blast_Score=310, Evalue=2e-84,
Organism=Drosophila melanogaster, GI21355181, Length=549, Percent_Identity=23.3151183970856, Blast_Score=100, Evalue=3e-21,
Organism=Drosophila melanogaster, GI21356441, Length=391, Percent_Identity=26.0869565217391, Blast_Score=96, Evalue=6e-20,
Organism=Drosophila melanogaster, GI24648253, Length=366, Percent_Identity=26.5027322404372, Blast_Score=96, Evalue=1e-19,
Organism=Drosophila melanogaster, GI24648255, Length=366, Percent_Identity=26.775956284153, Blast_Score=94, Evalue=2e-19,
Organism=Drosophila melanogaster, GI24648257, Length=402, Percent_Identity=24.8756218905473, Blast_Score=90, Evalue=5e-18,
Organism=Drosophila melanogaster, GI24648260, Length=360, Percent_Identity=24.1666666666667, Blast_Score=89, Evalue=1e-17,
Organism=Drosophila melanogaster, GI21356947, Length=360, Percent_Identity=23.0555555555556, Blast_Score=78, Evalue=2e-14,
Organism=Drosophila melanogaster, GI281365686, Length=457, Percent_Identity=22.7571115973742, Blast_Score=78, Evalue=2e-14,
Organism=Drosophila melanogaster, GI18859661, Length=136, Percent_Identity=35.2941176470588, Blast_Score=75, Evalue=1e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020845
- InterPro:   IPR000873 [H]

Pfam domain/function: PF00501 AMP-binding [H]

EC number: 6.2.1.17 [C]

Molecular weight: Translated: 68760; Mature: 68629

Theoretical pI: Translated: 5.39; Mature: 5.39

Prosite motif: PS00455 AMP_BINDING

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTNAYEEAYQRSITDKEGFWGDAARDIVWTKPWDRVLDESQAPLYRWFAGGELNTCYNAV
CCCHHHHHHHHCCCCCCCCCCCCCCCCEECCCHHHHHCCCCCCEEEEECCCCHHHHHHHH
DRHVDEGRGDQAAIIYDSAITGAKRTISYKTLKDQVAGFAGVLASRGVGKGDRVILYMPM
HHHHHCCCCCCEEEEEECCCCCCHHEEEHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCC
IPESLVAMLACARLGAIHSVVFGGFAPNELATRINDAQPKAIVAASCGIEPNRVIAYKPL
CHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHCCCCCCCEEEEEECCCCCCEEEEECCH
VDEAIRLSAHKPDSVIVWQREEARADLSNPGDIDWALALETTTPHDCVPVAATDPLYILY
HHHHHHHCCCCCCEEEEEECHHHHCCCCCCCCCEEEEEEECCCCCCCEEEECCCCEEEEE
TSGTTGQPKGVVRDNGGHAVALRWTMKAIYNMSPGDVFWAASDVGWVVGHSYICYAPLLL
ECCCCCCCCCCEECCCCCEEEEEEEEHHHHCCCCCCEEEEECCCCHHHCCCHHHHHHHHH
GATTLVFEGKPVGTPDAGTFWRVIAEHKVATLFTAPTAFRAIKREDPDGLEIGKYDLSSL
CCEEEEECCCCCCCCCCCHHHHHHHHCCEEEEEECCHHHHHHHCCCCCCCEECCCCHHHH
RALFLAGERCDPATIDWAEHKLGVPVIDHWWQTETGWAIAANCLGLHLYPVKHGSPTLAA
HHHHHCCCCCCCCCCCHHHHCCCCCHHHHCCCCCCCCEEEEEEEEEEEEEECCCCCCEEC
PGWDVRVLDDTNHEVNPGQIGALVCKLPLPPGTLTTLWNAEARFRQAYLADFPGYYKSGD
CCCEEEEEECCCCCCCCCCEEEEEEEECCCCCCEEEEECCHHHHHHHHHHHCCCCCCCCC
AGFIDDEGYIYVMTRTDDIINVAGHRLSTGAMEEVLAGHPAVAECAVIGISDDLKGQVPL
CCEECCCCEEEEEECCCCHHHHCCCCCCHHHHHHHHCCCCCHHEEEEEECCCCCCCCCCE
GFVCLKAGITTEPAQIAKECAALVRDQIGPVAAYKQTVVVPRLPKTRSGKILRGTMQKIA
EEEEEECCCCCCHHHHHHHHHHHHHHCCCCHHHHHCEEEECCCCCCCCCCHHHHHHHHHC
DNEAFKMPATIDDPGILPEIEEALESIGLARKRV
CCCCEECCCCCCCCCCCHHHHHHHHHHCHHHCCC
>Mature Secondary Structure 
TNAYEEAYQRSITDKEGFWGDAARDIVWTKPWDRVLDESQAPLYRWFAGGELNTCYNAV
CCHHHHHHHHCCCCCCCCCCCCCCCCEECCCHHHHHCCCCCCEEEEECCCCHHHHHHHH
DRHVDEGRGDQAAIIYDSAITGAKRTISYKTLKDQVAGFAGVLASRGVGKGDRVILYMPM
HHHHHCCCCCCEEEEEECCCCCCHHEEEHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCC
IPESLVAMLACARLGAIHSVVFGGFAPNELATRINDAQPKAIVAASCGIEPNRVIAYKPL
CHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHCCCCCCCEEEEEECCCCCCEEEEECCH
VDEAIRLSAHKPDSVIVWQREEARADLSNPGDIDWALALETTTPHDCVPVAATDPLYILY
HHHHHHHCCCCCCEEEEEECHHHHCCCCCCCCCEEEEEEECCCCCCCEEEECCCCEEEEE
TSGTTGQPKGVVRDNGGHAVALRWTMKAIYNMSPGDVFWAASDVGWVVGHSYICYAPLLL
ECCCCCCCCCCEECCCCCEEEEEEEEHHHHCCCCCCEEEEECCCCHHHCCCHHHHHHHHH
GATTLVFEGKPVGTPDAGTFWRVIAEHKVATLFTAPTAFRAIKREDPDGLEIGKYDLSSL
CCEEEEECCCCCCCCCCCHHHHHHHHCCEEEEEECCHHHHHHHCCCCCCCEECCCCHHHH
RALFLAGERCDPATIDWAEHKLGVPVIDHWWQTETGWAIAANCLGLHLYPVKHGSPTLAA
HHHHHCCCCCCCCCCCHHHHCCCCCHHHHCCCCCCCCEEEEEEEEEEEEEECCCCCCEEC
PGWDVRVLDDTNHEVNPGQIGALVCKLPLPPGTLTTLWNAEARFRQAYLADFPGYYKSGD
CCCEEEEEECCCCCCCCCCEEEEEEEECCCCCCEEEEECCHHHHHHHHHHHCCCCCCCCC
AGFIDDEGYIYVMTRTDDIINVAGHRLSTGAMEEVLAGHPAVAECAVIGISDDLKGQVPL
CCEECCCCEEEEEECCCCHHHHCCCCCCHHHHHHHHCCCCCHHEEEEEECCCCCCCCCCE
GFVCLKAGITTEPAQIAKECAALVRDQIGPVAAYKQTVVVPRLPKTRSGKILRGTMQKIA
EEEEEECCCCCCHHHHHHHHHHHHHHCCCCHHHHHCEEEECCCCCCCCCCHHHHHHHHHC
DNEAFKMPATIDDPGILPEIEEALESIGLARKRV
CCCCEECCCCCCCCCCCHHHHHHHHHHCHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10984043; 1339433 [H]