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The map label for this gene is hydN [H]

Identifier: 83591649

GI number: 83591649

Start: 377224

End: 377862

Strand: Direct

Name: hydN [H]

Synonym: Rru_A0309

Alternate gene names: 83591649

Gene position: 377224-377862 (Clockwise)

Preceding gene: 83591643

Following gene: 83591650

Centisome position: 8.67

GC content: 64.01

Gene sequence:

>639_bases
ATGGTGCGTCCGTCCTCGGCCGTGGCTGGTGGATGCTGTCTGGAGGTCCGACTGTTGAAACCCGATTCGCTGAATTACTT
CATTCTTGCCGATAGCGACAAATGTATTGGCTGTCGGATGTGTGAAATTGCCTGCGCCGTTACTCATTCGGAAGACAAGC
CCGAAACGGTGGGCGCTCTGGACGGTCCTTTGGTGCCCCGGCTGTTCGTCGTCGTCACCGACGACGTGACGGTGCCGGTG
ATCTGTCGTCACTGCGAGGATGCCCCTTGCGCCTCGGTCTGCAAGATGGCGGCGATCTCGCGGGTTGACGGCAAGGTCCT
GGTCGATGCCGAGCGCTGCGTCGGCTGCCGGTTGTGCCTGATGGCTTGCCCCTTCGGCGCCACCGAATTCGTCCCCCAGC
CCGCCGATGCCGCGCCCGTCTATATCACCCCGGCCGATGGCCGGCCGAGCCGCGCCGTCAAGGTCCGTTACAAAGCCAAT
AAATGCGACCTGTGCAGCGGCCTCGCCGATGGCCCGGCCTGCGTCAACGCCTGTCCCCAGAACGTCCTGTCGATCGTCGA
TCCCGCCGCCGAAGTGGCGCGGCGCGCCCTGGGCGCCGTCGAGGATCTGGTGTCCAGCATGAAATTCTCCGGTCTGTGA

Upstream 100 bases:

>100_bases
GCATTTAAGGGACCAGCTAAGCCGTTCCCGTGACCAATGAGGCCTGCCTAAACTTAAGGTCCTTTCCCCCCTAAGCAATA
CATCCTTCGTCAAGACTAAA

Downstream 100 bases:

>100_bases
GCCAGAGGGATATGAGCATGTCGATCCAACCGACCGTAACCATCGATCCCGAGCTGTGTACCGGATGTGGGCGGTGTGTT
GAAACCTGTCCGGTTCAGGC

Product: 4Fe-4S ferredoxin, iron-sulfur binding

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 212; Mature: 212

Protein sequence:

>212_residues
MVRPSSAVAGGCCLEVRLLKPDSLNYFILADSDKCIGCRMCEIACAVTHSEDKPETVGALDGPLVPRLFVVVTDDVTVPV
ICRHCEDAPCASVCKMAAISRVDGKVLVDAERCVGCRLCLMACPFGATEFVPQPADAAPVYITPADGRPSRAVKVRYKAN
KCDLCSGLADGPACVNACPQNVLSIVDPAAEVARRALGAVEDLVSSMKFSGL

Sequences:

>Translated_212_residues
MVRPSSAVAGGCCLEVRLLKPDSLNYFILADSDKCIGCRMCEIACAVTHSEDKPETVGALDGPLVPRLFVVVTDDVTVPV
ICRHCEDAPCASVCKMAAISRVDGKVLVDAERCVGCRLCLMACPFGATEFVPQPADAAPVYITPADGRPSRAVKVRYKAN
KCDLCSGLADGPACVNACPQNVLSIVDPAAEVARRALGAVEDLVSSMKFSGL
>Mature_212_residues
MVRPSSAVAGGCCLEVRLLKPDSLNYFILADSDKCIGCRMCEIACAVTHSEDKPETVGALDGPLVPRLFVVVTDDVTVPV
ICRHCEDAPCASVCKMAAISRVDGKVLVDAERCVGCRLCLMACPFGATEFVPQPADAAPVYITPADGRPSRAVKVRYKAN
KCDLCSGLADGPACVNACPQNVLSIVDPAAEVARRALGAVEDLVSSMKFSGL

Specific function: Electron transport from formate to hydrogen [H]

COG id: COG1142

COG function: function code C; Fe-S-cluster-containing hydrogenase components 2

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 4 4Fe-4S ferredoxin-type domains [H]

Homologues:

Organism=Escherichia coli, GI1789067, Length=170, Percent_Identity=46.4705882352941, Blast_Score=141, Evalue=3e-35,
Organism=Escherichia coli, GI2367245, Length=164, Percent_Identity=42.0731707317073, Blast_Score=128, Evalue=3e-31,
Organism=Escherichia coli, GI1788811, Length=167, Percent_Identity=38.9221556886228, Blast_Score=128, Evalue=3e-31,
Organism=Escherichia coli, GI1789079, Length=186, Percent_Identity=42.4731182795699, Blast_Score=127, Evalue=5e-31,
Organism=Escherichia coli, GI87082114, Length=203, Percent_Identity=33.9901477832512, Blast_Score=115, Evalue=3e-27,
Organism=Escherichia coli, GI87082179, Length=163, Percent_Identity=33.1288343558282, Blast_Score=103, Evalue=9e-24,
Organism=Escherichia coli, GI87082180, Length=165, Percent_Identity=34.5454545454545, Blast_Score=101, Evalue=4e-23,
Organism=Escherichia coli, GI1787872, Length=181, Percent_Identity=29.8342541436464, Blast_Score=72, Evalue=3e-14,
Organism=Escherichia coli, GI1787122, Length=181, Percent_Identity=29.2817679558011, Blast_Score=71, Evalue=5e-14,
Organism=Escherichia coli, GI226510944, Length=173, Percent_Identity=32.9479768786127, Blast_Score=69, Evalue=2e-13,
Organism=Escherichia coli, GI2367345, Length=196, Percent_Identity=30.1020408163265, Blast_Score=62, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001450
- InterPro:   IPR017896
- InterPro:   IPR017900 [H]

Pfam domain/function: PF00037 Fer4 [H]

EC number: NA

Molecular weight: Translated: 22276; Mature: 22276

Theoretical pI: Translated: 5.31; Mature: 5.31

Prosite motif: PS00198 4FE4S_FERREDOXIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

8.5 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
10.8 %Cys+Met (Translated Protein)
8.5 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
10.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVRPSSAVAGGCCLEVRLLKPDSLNYFILADSDKCIGCRMCEIACAVTHSEDKPETVGAL
CCCCCCCCCCCEEEEEEEECCCCCCEEEEECCCCCCCEEHEEEEEEEECCCCCCCCCCCC
DGPLVPRLFVVVTDDVTVPVICRHCEDAPCASVCKMAAISRVDGKVLVDAERCVGCRLCL
CCCCCCEEEEEEECCCCHHHHHHCCCCCCHHHHHHHHHHHHCCCEEEEEHHHHHHHHHHH
MACPFGATEFVPQPADAAPVYITPADGRPSRAVKVRYKANKCDLCSGLADGPACVNACPQ
HHCCCCCHHCCCCCCCCCCEEEECCCCCCCCEEEEEEECCCCCHHCCCCCCHHHHHHCHH
NVLSIVDPAAEVARRALGAVEDLVSSMKFSGL
HHHHHHCHHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MVRPSSAVAGGCCLEVRLLKPDSLNYFILADSDKCIGCRMCEIACAVTHSEDKPETVGAL
CCCCCCCCCCCEEEEEEEECCCCCCEEEEECCCCCCCEEHEEEEEEEECCCCCCCCCCCC
DGPLVPRLFVVVTDDVTVPVICRHCEDAPCASVCKMAAISRVDGKVLVDAERCVGCRLCL
CCCCCCEEEEEEECCCCHHHHHHCCCCCCHHHHHHHHHHHHCCCEEEEEHHHHHHHHHHH
MACPFGATEFVPQPADAAPVYITPADGRPSRAVKVRYKANKCDLCSGLADGPACVNACPQ
HHCCCCCHHCCCCCCCCCCEEEECCCCCCCCEEEEEEECCCCCHHCCCCCCHHHHHHCHH
NVLSIVDPAAEVARRALGAVEDLVSSMKFSGL
HHHHHHCHHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: Fe [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]