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The map label for this gene is hypB [H]

Identifier: 83591642

GI number: 83591642

Start: 368703

End: 369593

Strand: Direct

Name: hypB [H]

Synonym: Rru_A0302

Alternate gene names: 83591642

Gene position: 368703-369593 (Clockwise)

Preceding gene: 83591641

Following gene: 83591643

Centisome position: 8.47

GC content: 64.65

Gene sequence:

>891_bases
ATGTGCGAGACCTGCGGTTGCGCCGGCACCGGCCCCCATCATGTTCACGGCGACCACGCCCACAGCCATTCCCACGGCGA
CGGCCACACCCATGATCACGCCCACGACCATGGGCATGGCCATTCCCACGACCACGATCACGATCACGGGCATGGTCATT
CCCATTCCCACGACCACGACCACGGGCATTCCCATTCCCACGGTTCCGCCTCGGAGGGGACGCGGACGGTCATCGTTCTT
GAAGACCTTTTGGCCAAGAACGACCATCAGGCGGCCCATGTGCGCGCCCATTTCGACGCCCGGGGCATTCTGGCGGTCAA
CCTGATGTCCTCGCCGGGCAGCGGCAAGACCAGCTTGCTTGAAGCCACCATCCAGGCGCTGCCCGCCGGGGTGCGGGTGG
CGGTGATCGAGGGCGATCTGGAAACCGAGAACGATGCCGAGCGCATCCGCCGCCATGGCGTGCCGGCGGTTCAGATCACC
ACCGGCACCGCCTGCCACCTTGATGCCCATATGATCCATGATGCCCTGCACCACCTGGATCTTGACGGCATCGATATCGT
CTTCATCGAGAATGTCGGCAATCTGGTCTGCCCGGCGACCTTCGATATCGGCCAGCACCGCAATGTCGTGCTGCTGTCGG
TGACCGAAGGCGATGACAAGCCGGCGAAATATCCGGTGATCATGCGCGCCGCCGACCGGGTGCTGATCACCAAGGCCGAT
CTTTTGCCCCATATCGAGGAATTCGACGTCGAGCGCGCCCGCGCCTCGATCGCCGGCGTCGCCGGACCGGTGCCGGTTTG
CGTGGTTTCGTCCAAACGCGGTCCGGGCATGGATGACTGGATCGGCTGGCTGATGGCCGAACACGCCGCCCGCAAGCCAG
CCGTCGCCTGA

Upstream 100 bases:

>100_bases
CTTCGGAGTAGGGTTGGCTGGCCCGCCCTCGCGCCCCTTTCGCGGCCGGGCCGAAGGACGGGGCGTCCCCCCCAGACCGT
TTTCCCCCGAGGATCTTCCC

Downstream 100 bases:

>100_bases
GCGCGGGTCACTAAGACGGGGAGGCGCGCGGAAACCCGGGATCGAGGGGTCCGCGCGCCGCTGGTTCATCGGGCTATACG
ATGGATGCTTCGGTTTGGGA

Product: hydrogenase accessory protein HypB

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 296; Mature: 296

Protein sequence:

>296_residues
MCETCGCAGTGPHHVHGDHAHSHSHGDGHTHDHAHDHGHGHSHDHDHDHGHGHSHSHDHDHGHSHSHGSASEGTRTVIVL
EDLLAKNDHQAAHVRAHFDARGILAVNLMSSPGSGKTSLLEATIQALPAGVRVAVIEGDLETENDAERIRRHGVPAVQIT
TGTACHLDAHMIHDALHHLDLDGIDIVFIENVGNLVCPATFDIGQHRNVVLLSVTEGDDKPAKYPVIMRAADRVLITKAD
LLPHIEEFDVERARASIAGVAGPVPVCVVSSKRGPGMDDWIGWLMAEHAARKPAVA

Sequences:

>Translated_296_residues
MCETCGCAGTGPHHVHGDHAHSHSHGDGHTHDHAHDHGHGHSHDHDHDHGHGHSHSHDHDHGHSHSHGSASEGTRTVIVL
EDLLAKNDHQAAHVRAHFDARGILAVNLMSSPGSGKTSLLEATIQALPAGVRVAVIEGDLETENDAERIRRHGVPAVQIT
TGTACHLDAHMIHDALHHLDLDGIDIVFIENVGNLVCPATFDIGQHRNVVLLSVTEGDDKPAKYPVIMRAADRVLITKAD
LLPHIEEFDVERARASIAGVAGPVPVCVVSSKRGPGMDDWIGWLMAEHAARKPAVA
>Mature_296_residues
MCETCGCAGTGPHHVHGDHAHSHSHGDGHTHDHAHDHGHGHSHDHDHDHGHGHSHSHDHDHGHSHSHGSASEGTRTVIVL
EDLLAKNDHQAAHVRAHFDARGILAVNLMSSPGSGKTSLLEATIQALPAGVRVAVIEGDLETENDAERIRRHGVPAVQIT
TGTACHLDAHMIHDALHHLDLDGIDIVFIENVGNLVCPATFDIGQHRNVVLLSVTEGDDKPAKYPVIMRAADRVLITKAD
LLPHIEEFDVERARASIAGVAGPVPVCVVSSKRGPGMDDWIGWLMAEHAARKPAVA

Specific function: Required for the maturation of the three [NiFe]hydrogenases. Exhibits a low intrinsic GTPase activity. The GTP hydrolysis catalyzed by HypB is an integral process in the incorporation of nickel into hydrogenases [H]

COG id: COG0378

COG function: function code OK; Ni2+-binding GTPase involved in regulation of expression and maturation of urease and hydrogenase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the hypB/hupM family [H]

Homologues:

Organism=Escherichia coli, GI1789082, Length=292, Percent_Identity=43.8356164383562, Blast_Score=233, Evalue=1e-62,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003495
- InterPro:   IPR004392
- InterPro:   IPR012202 [H]

Pfam domain/function: PF02492 cobW [H]

EC number: NA

Molecular weight: Translated: 31707; Mature: 31707

Theoretical pI: Translated: 6.48; Mature: 6.48

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MCETCGCAGTGPHHVHGDHAHSHSHGDGHTHDHAHDHGHGHSHDHDHDHGHGHSHSHDHD
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
HGHSHSHGSASEGTRTVIVLEDLLAKNDHQAAHVRAHFDARGILAVNLMSSPGSGKTSLL
CCCCCCCCCCCCCCEEEEEEHHHHCCCCCCEEEEEEECCCCCEEEEEEECCCCCCHHHHH
EATIQALPAGVRVAVIEGDLETENDAERIRRHGVPAVQITTGTACHLDAHMIHDALHHLD
HHHHHHCCCCEEEEEEECCCCCCHHHHHHHHCCCCEEEEECCCEEEHHHHHHHHHHHHCC
LDGIDIVFIENVGNLVCPATFDIGQHRNVVLLSVTEGDDKPAKYPVIMRAADRVLITKAD
CCCEEEEEEECCCCEECCEEECCCCCCCEEEEEEECCCCCCCCCCEEEECCCEEEEEEHH
LLPHIEEFDVERARASIAGVAGPVPVCVVSSKRGPGMDDWIGWLMAEHAARKPAVA
HCCCHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MCETCGCAGTGPHHVHGDHAHSHSHGDGHTHDHAHDHGHGHSHDHDHDHGHGHSHSHDHD
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
HGHSHSHGSASEGTRTVIVLEDLLAKNDHQAAHVRAHFDARGILAVNLMSSPGSGKTSLL
CCCCCCCCCCCCCCEEEEEEHHHHCCCCCCEEEEEEECCCCCEEEEEEECCCCCCHHHHH
EATIQALPAGVRVAVIEGDLETENDAERIRRHGVPAVQITTGTACHLDAHMIHDALHHLD
HHHHHHCCCCEEEEEEECCCCCCHHHHHHHHCCCCEEEEECCCEEEHHHHHHHHHHHHCC
LDGIDIVFIENVGNLVCPATFDIGQHRNVVLLSVTEGDDKPAKYPVIMRAADRVLITKAD
CCCEEEEEEECCCCEECCEEECCCCCCCEEEEEEECCCCCCCCCCEEEECCCEEEEEEHH
LLPHIEEFDVERARASIAGVAGPVPVCVVSSKRGPGMDDWIGWLMAEHAARKPAVA
HCCCHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]