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The map label for this gene is folE [H]

Identifier: 83591611

GI number: 83591611

Start: 336213

End: 336917

Strand: Direct

Name: folE [H]

Synonym: Rru_A0271

Alternate gene names: 83591611

Gene position: 336213-336917 (Clockwise)

Preceding gene: 83591610

Following gene: 83591612

Centisome position: 7.72

GC content: 65.96

Gene sequence:

>705_bases
GTGACGACCAATCTCCCGGCTGACACCGCCGAAGCAAGCCTTTGCGCCTTCCGCGCCGATGGCGAAGAAGGGATCCCGGC
GACGCCTGTCGTGGCGGACGGCCTTTGGCCCACCACAAAGCCCTCGCGGGCCGAAGCCGAGGCGGCGGTGCGGACCCTGA
TCCGCTGGGCCGGCGATGATCCCAATCGCGAAGGCCTGATCGACACCCCGGCGCGGGTGGCGCGATCCTACGAGGAATTG
TTTTCCGGCTATGGCGCCGATCCCGAGGACCTGCTGCGCCGCACCTTCGAAGAGGTCGAGGGCTACGACGAGGTGGTGCT
GCTGCGCGATATCCGCATCGAAAGCCATTGCGAACACCATATGGTGCCGATCATCGGCAAGGTGCACATCGGCTATCTGC
CCAATCGGCGGGTTGTGGGCATTTCCAAGCTGGCCCGCGTTGCCGAGGTCTATGCCAAGCGGCTGCAGATCCAGGAAAAG
ATGACCGCCCAGATCGCCAATGCCATCAACGCCGTGCTCGAACCGCGCGGCGTGGCCGTGGTGGTCGAGGCCGCCCACCA
GTGCATGACGACGCGCGGCGTTCACAAGCCCGGCGTCACCATGGTGACCAGCCGCATGCTCGGCGCCTTCCGCACGAACT
CCGACACCCGCCGCGAGTTCCTCCACCTGATCGGTCAAACCCGCGAAGGCGGTTTCGGCCTCTGA

Upstream 100 bases:

>100_bases
TGTAGGACCCTGCGGGGTAAGGCGGTCCCGAGGCGGCGGGCGCGTGGCGCGTCGGCATCGGACCGTTCCCTGACGCGGGC
ATGGAAAGGATCCCCCTATC

Downstream 100 bases:

>100_bases
GGCGAGACTTGTGTTCGTCAGGCGGGGACGACCCCGCTGGAGGTGTTTTTCAGGCGGGGACGACCCCGCCATGGATCCAG
AGGGAGGCATCCATGGCTTG

Product: GTP cyclohydrolase I

Products: NA

Alternate protein names: GTP cyclohydrolase I; GTP-CH-I [H]

Number of amino acids: Translated: 234; Mature: 233

Protein sequence:

>234_residues
MTTNLPADTAEASLCAFRADGEEGIPATPVVADGLWPTTKPSRAEAEAAVRTLIRWAGDDPNREGLIDTPARVARSYEEL
FSGYGADPEDLLRRTFEEVEGYDEVVLLRDIRIESHCEHHMVPIIGKVHIGYLPNRRVVGISKLARVAEVYAKRLQIQEK
MTAQIANAINAVLEPRGVAVVVEAAHQCMTTRGVHKPGVTMVTSRMLGAFRTNSDTRREFLHLIGQTREGGFGL

Sequences:

>Translated_234_residues
MTTNLPADTAEASLCAFRADGEEGIPATPVVADGLWPTTKPSRAEAEAAVRTLIRWAGDDPNREGLIDTPARVARSYEEL
FSGYGADPEDLLRRTFEEVEGYDEVVLLRDIRIESHCEHHMVPIIGKVHIGYLPNRRVVGISKLARVAEVYAKRLQIQEK
MTAQIANAINAVLEPRGVAVVVEAAHQCMTTRGVHKPGVTMVTSRMLGAFRTNSDTRREFLHLIGQTREGGFGL
>Mature_233_residues
TTNLPADTAEASLCAFRADGEEGIPATPVVADGLWPTTKPSRAEAEAAVRTLIRWAGDDPNREGLIDTPARVARSYEELF
SGYGADPEDLLRRTFEEVEGYDEVVLLRDIRIESHCEHHMVPIIGKVHIGYLPNRRVVGISKLARVAEVYAKRLQIQEKM
TAQIANAINAVLEPRGVAVVVEAAHQCMTTRGVHKPGVTMVTSRMLGAFRTNSDTRREFLHLIGQTREGGFGL

Specific function: Tetrahydrofolate biosynthesis; first step. [C]

COG id: COG0302

COG function: function code H; GTP cyclohydrolase I

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP cyclohydrolase I family [H]

Homologues:

Organism=Homo sapiens, GI66932968, Length=177, Percent_Identity=50.2824858757062, Blast_Score=183, Evalue=1e-46,
Organism=Homo sapiens, GI4503949, Length=177, Percent_Identity=50.2824858757062, Blast_Score=183, Evalue=1e-46,
Organism=Homo sapiens, GI66932970, Length=147, Percent_Identity=46.2585034013605, Blast_Score=142, Evalue=2e-34,
Organism=Homo sapiens, GI66932972, Length=137, Percent_Identity=48.9051094890511, Blast_Score=140, Evalue=1e-33,
Organism=Escherichia coli, GI1788476, Length=204, Percent_Identity=34.3137254901961, Blast_Score=119, Evalue=2e-28,
Organism=Caenorhabditis elegans, GI17560486, Length=178, Percent_Identity=47.752808988764, Blast_Score=187, Evalue=3e-48,
Organism=Saccharomyces cerevisiae, GI6321706, Length=180, Percent_Identity=52.2222222222222, Blast_Score=195, Evalue=6e-51,
Organism=Drosophila melanogaster, GI24656782, Length=211, Percent_Identity=43.1279620853081, Blast_Score=164, Evalue=4e-41,
Organism=Drosophila melanogaster, GI24656791, Length=211, Percent_Identity=43.1279620853081, Blast_Score=163, Evalue=8e-41,
Organism=Drosophila melanogaster, GI24656796, Length=211, Percent_Identity=43.1279620853081, Blast_Score=163, Evalue=8e-41,

Paralogues:

None

Copy number: 420 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001474
- InterPro:   IPR020602
- InterPro:   IPR018234 [H]

Pfam domain/function: PF01227 GTP_cyclohydroI [H]

EC number: =3.5.4.16 [H]

Molecular weight: Translated: 25757; Mature: 25625

Theoretical pI: Translated: 6.40; Mature: 6.40

Prosite motif: PS00859 GTP_CYCLOHYDROL_1_1 ; PS00860 GTP_CYCLOHYDROL_1_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTNLPADTAEASLCAFRADGEEGIPATPVVADGLWPTTKPSRAEAEAAVRTLIRWAGDD
CCCCCCCCHHHHHHHEEECCCCCCCCCCCEEECCCCCCCCCCHHHHHHHHHHHHHHCCCC
PNREGLIDTPARVARSYEELFSGYGADPEDLLRRTFEEVEGYDEVVLLRDIRIESHCEHH
CCCCCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCEEEHHHHCHHHHCCCC
MVPIIGKVHIGYLPNRRVVGISKLARVAEVYAKRLQIQEKMTAQIANAINAVLEPRGVAV
CCHHHHEEEECCCCCCCEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHH
VVEAAHQCMTTRGVHKPGVTMVTSRMLGAFRTNSDTRREFLHLIGQTREGGFGL
HHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure 
TTNLPADTAEASLCAFRADGEEGIPATPVVADGLWPTTKPSRAEAEAAVRTLIRWAGDD
CCCCCCCHHHHHHHEEECCCCCCCCCCCEEECCCCCCCCCCHHHHHHHHHHHHHHCCCC
PNREGLIDTPARVARSYEELFSGYGADPEDLLRRTFEEVEGYDEVVLLRDIRIESHCEHH
CCCCCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCEEEHHHHCHHHHCCCC
MVPIIGKVHIGYLPNRRVVGISKLARVAEVYAKRLQIQEKMTAQIANAINAVLEPRGVAV
CCHHHHEEEECCCCCCCEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHH
VVEAAHQCMTTRGVHKPGVTMVTSRMLGAFRTNSDTRREFLHLIGQTREGGFGL
HHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12024217 [H]