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The map label for this gene is cysU [H]

Identifier: 83312665

GI number: 83312665

Start: 3920662

End: 3921498

Strand: Reverse

Name: cysU [H]

Synonym: amb3566

Alternate gene names: 83312665

Gene position: 3921498-3920662 (Counterclockwise)

Preceding gene: 83312666

Following gene: 83312664

Centisome position: 78.95

GC content: 67.5

Gene sequence:

>837_bases
ATGATGCGCGCCCGCAGGCTTTTGCCCGGCTTCTCGTTGACCATGGGTATGACGCTGGCCTATCTCGGCCTGATCGTCCT
GCTGCCCCTGGGCGCCATGCTGGTCAAGGCGGCGGGCATGAGCCTGGAGGGTTTCATCGCCGCCACCACCGGGCCGCGCG
CCCTGGCCGCCTATCAGGTCACCCTGGTGTCGGCGGCCGAGGCGGTGATCTTCAACGGTCTGGCCGGGCTGCTGTTCGCC
TGGGTGCTGGCCCGCTATCAGTTCGCCGGGCGCAACCTGCTGGACGCCCTGATTGACCTGCCCTTCGCCATGCCCACCGC
CGTGGCGGGCCTGGCCCTGGCCGCCGCCTTCGCTCCCAACGGCTGGGTGGGTCAGCATCTGGAGCCCCTGGGCTTCAAGG
TGGTCCATGCCCAGCCGGGTATCGCCATCGCCATGGCCTTCACCAGCCTGCCCTTCGTGGTGCGAAGCGTGCAGCCGGTG
ATCGAGGAACTGGAGACCGAGACCGAGGAGGCGGCCCGCACCCTGGGCGCTTCCGAGATGCAGATTTTCCTGAAGGTGAT
CCTGCCCACCCTGGCGCCATCGCTGCTGGCCGGCATGTCCCCTGGCCTTCGCCCGCTGCCTGGGCGAGTTCGGCGCCATC
ATCTTCATCGCCGGCAACCGCCCGTTCGAGACCGAGATCGCCGCCCTGCTGATCTTCATCAAGCTGGAGGAATACGAATT
CGGCTCGGCCACCGCCATCGCCACCGTGGTGCTGGCCTTCGCCTTCGTCACCATGCTGCTGACCAACGGCATCCAGGCCT
GGCAGCAGCGCTACCTGGCAAAGGGGTGAGACGATGA

Upstream 100 bases:

>100_bases
CGGTAAGAAGTGATGCGTCTCCGGCGCGATCCGGCTAAAAGGTCTCTCACCCGGCGGTGCTCCGCCGGGTGATGGCCAGA
CAGGTCCCACCGGAGTTTGC

Downstream 100 bases:

>100_bases
TTCCCGCCATGGCCGTTCACCACGTTCCCCGCCGTCGTCCCGGCGGTGCCCGGCGCGCCCTGCTGATCGGGCTGGCCGGG
CTGCTGGCCCTGGGCTTCCT

Product: ABC-type sulfate transport system, permease component

Products: ADP; phosphate; S2O32- [Cytoplasm]; SO42- [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 278; Mature: 278

Protein sequence:

>278_residues
MMRARRLLPGFSLTMGMTLAYLGLIVLLPLGAMLVKAAGMSLEGFIAATTGPRALAAYQVTLVSAAEAVIFNGLAGLLFA
WVLARYQFAGRNLLDALIDLPFAMPTAVAGLALAAAFAPNGWVGQHLEPLGFKVVHAQPGIAIAMAFTSLPFVVRSVQPV
IEELETETEEAARTLGASEMQIFLKVILPTLAPSLLAGMSPGLRPLPGRVRRHHLHRRQPPVRDRDRRPADLHQAGGIRI
RLGHRHRHRGAGLRLRHHAADQRHPGLAAALPGKGVRR

Sequences:

>Translated_278_residues
MMRARRLLPGFSLTMGMTLAYLGLIVLLPLGAMLVKAAGMSLEGFIAATTGPRALAAYQVTLVSAAEAVIFNGLAGLLFA
WVLARYQFAGRNLLDALIDLPFAMPTAVAGLALAAAFAPNGWVGQHLEPLGFKVVHAQPGIAIAMAFTSLPFVVRSVQPV
IEELETETEEAARTLGASEMQIFLKVILPTLAPSLLAGMSPGLRPLPGRVRRHHLHRRQPPVRDRDRRPADLHQAGGIRI
RLGHRHRHRGAGLRLRHHAADQRHPGLAAALPGKGVRR
>Mature_278_residues
MMRARRLLPGFSLTMGMTLAYLGLIVLLPLGAMLVKAAGMSLEGFIAATTGPRALAAYQVTLVSAAEAVIFNGLAGLLFA
WVLARYQFAGRNLLDALIDLPFAMPTAVAGLALAAAFAPNGWVGQHLEPLGFKVVHAQPGIAIAMAFTSLPFVVRSVQPV
IEELETETEEAARTLGASEMQIFLKVILPTLAPSLLAGMSPGLRPLPGRVRRHHLHRRQPPVRDRDRRPADLHQAGGIRI
RLGHRHRHRGAGLRLRHHAADQRHPGLAAALPGKGVRR

Specific function: Part of the ABC transporter complex CysAWTP (TC 3.A.1.6.1) involved in sulfate/thiosulfate import. Probably responsible for the translocation of the substrate across the membrane [H]

COG id: COG0555

COG function: function code O; ABC-type sulfate transport system, permease component

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1788764, Length=199, Percent_Identity=56.2814070351759, Blast_Score=202, Evalue=1e-53,
Organism=Escherichia coli, GI87082099, Length=189, Percent_Identity=34.3915343915344, Blast_Score=104, Evalue=8e-24,
Organism=Escherichia coli, GI1786980, Length=145, Percent_Identity=33.1034482758621, Blast_Score=73, Evalue=2e-14,
Organism=Escherichia coli, GI1787369, Length=125, Percent_Identity=31.2, Blast_Score=64, Evalue=8e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515
- InterPro:   IPR011865
- InterPro:   IPR005667 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 29950; Mature: 29950

Theoretical pI: Translated: 12.12; Mature: 12.12

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMRARRLLPGFSLTMGMTLAYLGLIVLLPLGAMLVKAAGMSLEGFIAATTGPRALAAYQV
CCCCHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEECCCCHHHHHHHH
TLVSAAEAVIFNGLAGLLFAWVLARYQFAGRNLLDALIDLPFAMPTAVAGLALAAAFAPN
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCHHHHHHHHHHHHHHHHCCC
GWVGQHLEPLGFKVVHAQPGIAIAMAFTSLPFVVRSVQPVIEELETETEEAARTLGASEM
CCCCCCCCCCCEEEEECCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHH
QIFLKVILPTLAPSLLAGMSPGLRPLPGRVRRHHLHRRQPPVRDRDRRPADLHQAGGIRI
HHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCHHHHCCCEEE
RLGHRHRHRGAGLRLRHHAADQRHPGLAAALPGKGVRR
EECCCCCCCCCCCEEHHHCCCCCCCCCEEECCCCCCCC
>Mature Secondary Structure
MMRARRLLPGFSLTMGMTLAYLGLIVLLPLGAMLVKAAGMSLEGFIAATTGPRALAAYQV
CCCCHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEECCCCHHHHHHHH
TLVSAAEAVIFNGLAGLLFAWVLARYQFAGRNLLDALIDLPFAMPTAVAGLALAAAFAPN
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCHHHHHHHHHHHHHHHHCCC
GWVGQHLEPLGFKVVHAQPGIAIAMAFTSLPFVVRSVQPVIEELETETEEAARTLGASEM
CCCCCCCCCCCEEEEECCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHH
QIFLKVILPTLAPSLLAGMSPGLRPLPGRVRRHHLHRRQPPVRDRDRRPADLHQAGGIRI
HHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCHHHHCCCEEE
RLGHRHRHRGAGLRLRHHAADQRHPGLAAALPGKGVRR
EECCCCCCCCCCCEEHHHCCCCCCCCCEEECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; S2O32- [Periplasm]; H2O; SO42- [Periplasm]; ATP [C]

Specific reaction: ATP + S2O32- [Periplasm] + H2O = ADP + phosphate + S2O32- [Cytoplasm] SO42- [Periplasm] + H2O + ATP = SO42- [Cytoplasm] + phosphate + ADP [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 2188958; 9205837; 9278503; 2188959 [H]