Click here to switch to the map view.

The map label for this gene is cysP [H]

Identifier: 83312666

GI number: 83312666

Start: 3921586

End: 3922614

Strand: Reverse

Name: cysP [H]

Synonym: amb3567

Alternate gene names: 83312666

Gene position: 3922614-3921586 (Counterclockwise)

Preceding gene: 83312669

Following gene: 83312665

Centisome position: 78.97

GC content: 65.31

Gene sequence:

>1029_bases
ATGGAGTTTCCCATGGCTTTGCGCTTTCCCACTCTCGCCGTCCTGGCGGCCTTGGCCCTGGTTCCGGCCGGGGCCTCGGC
CGAGGACAAGGTGCCGGGCGAGTTGCTGAACGTGTCCTACGACATCGCCCGCGAACTGTTCCAGCAGATCAACCCCGCCT
TCCAGGCCACCCCGGCGGGCAAGCTCGCCGACGGCAAAGCCATCGAGATCAAGCAGAGCCACGCCGGCTCGTCCAAGCAG
GCCCGCGCCATCCTCGAAGGCCTGCCCGCCGACGTGGTGACCTTCAACCAGGTGACCGACGTCCAGGTCCTGGCCGACAA
GGGCTTCGTGCGCAAGGACTGGCAGACCGCCTTTCCCAGTGGCGCCTCGCCCTATTACTCGCTGCCGGCCTTCGTGGTGC
GAAAGGGCAACCCCAAGAACATCAGGAACTGGGACGATCTGGTCCGCCCGGATGTGAAGGTGGTGTTCCCCAACCCCAAG
ACCTCGGGCAACGGCCGCTACACCTATCTGGCGGCCTATGCCTTCGCGCTGGAGGCGGCCAAGGGCGATCAGGTCCAGGC
CAAGGAATTCATCCGCAAGCTGGTGGCCAACGTGCCGGTGTTCGACACCGGCGGCCGTGGCGCCACCACCACCTTCGTCG
AGCGGGCCACCGGCGACGTGCTGGTCACCTTCGAGGCCGAGGTGGGCGGCGTGATCAAGGAATTCGGCGCCGACAAGGTG
GAAGGCGTGATCCCGCCGGTCAGCATCCTGGCCGAATTCCCGGTGGCGGTGGTGGACAAGGTGGCGGCCAAGAAGGGCTC
GGCCAAGACCGCCAAGGCCTATCTCGACTTCCTGTATTCCGATGACGGACAGGAGATCCTGGCCAAAAACTTCTACCGGG
TGCGCTCGGAGAAGGTGGCGGCCCGGCATGCGGCCGCCTTCCCCAAGGTGCGCCTGTACACCACCGAGCTGTTCGGCGGC
TGGGACAACATCCAGAAGATCCACTTCGCCGAGGGCGGAATCTTCGATCAGGTCTTCGGTAAGAAGTGA

Upstream 100 bases:

>100_bases
TTTAGGTGAAGATGCGTATCCTCGCCGAAATAACTCATAAAAAATGTATTTTTAAAATAAACGACACATGATATTCGTGT
TTTAACCCAAGGATCACCGA

Downstream 100 bases:

>100_bases
TGCGTCTCCGGCGCGATCCGGCTAAAAGGTCTCTCACCCGGCGGTGCTCCGCCGGGTGATGGCCAGACAGGTCCCACCGG
AGTTTGCATGATGCGCGCCC

Product: ABC-type sulfate transport system, periplasmic component

Products: SO42- [Cytoplasm]; phosphate; ADP; S2O32- [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 342; Mature: 342

Protein sequence:

>342_residues
MEFPMALRFPTLAVLAALALVPAGASAEDKVPGELLNVSYDIARELFQQINPAFQATPAGKLADGKAIEIKQSHAGSSKQ
ARAILEGLPADVVTFNQVTDVQVLADKGFVRKDWQTAFPSGASPYYSLPAFVVRKGNPKNIRNWDDLVRPDVKVVFPNPK
TSGNGRYTYLAAYAFALEAAKGDQVQAKEFIRKLVANVPVFDTGGRGATTTFVERATGDVLVTFEAEVGGVIKEFGADKV
EGVIPPVSILAEFPVAVVDKVAAKKGSAKTAKAYLDFLYSDDGQEILAKNFYRVRSEKVAARHAAAFPKVRLYTTELFGG
WDNIQKIHFAEGGIFDQVFGKK

Sequences:

>Translated_342_residues
MEFPMALRFPTLAVLAALALVPAGASAEDKVPGELLNVSYDIARELFQQINPAFQATPAGKLADGKAIEIKQSHAGSSKQ
ARAILEGLPADVVTFNQVTDVQVLADKGFVRKDWQTAFPSGASPYYSLPAFVVRKGNPKNIRNWDDLVRPDVKVVFPNPK
TSGNGRYTYLAAYAFALEAAKGDQVQAKEFIRKLVANVPVFDTGGRGATTTFVERATGDVLVTFEAEVGGVIKEFGADKV
EGVIPPVSILAEFPVAVVDKVAAKKGSAKTAKAYLDFLYSDDGQEILAKNFYRVRSEKVAARHAAAFPKVRLYTTELFGG
WDNIQKIHFAEGGIFDQVFGKK
>Mature_342_residues
MEFPMALRFPTLAVLAALALVPAGASAEDKVPGELLNVSYDIARELFQQINPAFQATPAGKLADGKAIEIKQSHAGSSKQ
ARAILEGLPADVVTFNQVTDVQVLADKGFVRKDWQTAFPSGASPYYSLPAFVVRKGNPKNIRNWDDLVRPDVKVVFPNPK
TSGNGRYTYLAAYAFALEAAKGDQVQAKEFIRKLVANVPVFDTGGRGATTTFVERATGDVLVTFEAEVGGVIKEFGADKV
EGVIPPVSILAEFPVAVVDKVAAKKGSAKTAKAYLDFLYSDDGQEILAKNFYRVRSEKVAARHAAAFPKVRLYTTELFGG
WDNIQKIHFAEGGIFDQVFGKK

Specific function: Part of the ABC transporter complex CysAWTP (TC 3.A.1.6.1) involved in sulfate/thiosulfate import. This protein specifically binds thiosulfate and is involved in its transmembrane transport [H]

COG id: COG4150

COG function: function code P; ABC-type sulfate transport system, periplasmic component

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the prokaryotic sulfate-binding protein family [H]

Homologues:

Organism=Escherichia coli, GI1788765, Length=308, Percent_Identity=54.5454545454545, Blast_Score=336, Evalue=1e-93,
Organism=Escherichia coli, GI1790351, Length=306, Percent_Identity=50.9803921568627, Blast_Score=314, Evalue=6e-87,

Paralogues:

None

Copy number: 440 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006059
- InterPro:   IPR000957
- InterPro:   IPR005669 [H]

Pfam domain/function: PF01547 SBP_bac_1 [H]

EC number: NA

Molecular weight: Translated: 37034; Mature: 37034

Theoretical pI: Translated: 9.47; Mature: 9.47

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
0.6 %Met     (Translated Protein)
0.6 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
0.6 %Met     (Mature Protein)
0.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEFPMALRFPTLAVLAALALVPAGASAEDKVPGELLNVSYDIARELFQQINPAFQATPAG
CCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCHHHHHCCHHHHHHHHHHCCCCCCCCCCC
KLADGKAIEIKQSHAGSSKQARAILEGLPADVVTFNQVTDVQVLADKGFVRKDWQTAFPS
CCCCCCEEEEECCCCCCHHHHHHHHHCCCCCEEEECCCCCEEEEECCCCCCCCHHHCCCC
GASPYYSLPAFVVRKGNPKNIRNWDDLVRPDVKVVFPNPKTSGNGRYTYLAAYAFALEAA
CCCCCCCCCEEEEECCCCCCCCCHHHHCCCCEEEEECCCCCCCCCCEEHHHHHHHHHHHC
KGDQVQAKEFIRKLVANVPVFDTGGRGATTTFVERATGDVLVTFEAEVGGVIKEFGADKV
CCCCHHHHHHHHHHHHCCCEEECCCCCCHHHHHHHCCCCEEEEEEHHHHHHHHHHCCCHH
EGVIPPVSILAEFPVAVVDKVAAKKGSAKTAKAYLDFLYSDDGQEILAKNFYRVRSEKVA
HCCCCCHHHHHHCCHHHHHHHHHCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHH
ARHAAAFPKVRLYTTELFGGWDNIQKIHFAEGGIFDQVFGKK
HHHHHCCCCEEEEEEHHHCCHHHCCEEEECCCCCHHHHHCCC
>Mature Secondary Structure
MEFPMALRFPTLAVLAALALVPAGASAEDKVPGELLNVSYDIARELFQQINPAFQATPAG
CCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCHHHHHCCHHHHHHHHHHCCCCCCCCCCC
KLADGKAIEIKQSHAGSSKQARAILEGLPADVVTFNQVTDVQVLADKGFVRKDWQTAFPS
CCCCCCEEEEECCCCCCHHHHHHHHHCCCCCEEEECCCCCEEEEECCCCCCCCHHHCCCC
GASPYYSLPAFVVRKGNPKNIRNWDDLVRPDVKVVFPNPKTSGNGRYTYLAAYAFALEAA
CCCCCCCCCEEEEECCCCCCCCCHHHHCCCCEEEEECCCCCCCCCCEEHHHHHHHHHHHC
KGDQVQAKEFIRKLVANVPVFDTGGRGATTTFVERATGDVLVTFEAEVGGVIKEFGADKV
CCCCHHHHHHHHHHHHCCCEEECCCCCCHHHHHHHCCCCEEEEEEHHHHHHHHHHCCCHH
EGVIPPVSILAEFPVAVVDKVAAKKGSAKTAKAYLDFLYSDDGQEILAKNFYRVRSEKVA
HCCCCCHHHHHHCCHHHHHHHHHCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHH
ARHAAAFPKVRLYTTELFGGWDNIQKIHFAEGGIFDQVFGKK
HHHHHCCCCEEEEEEHHHCCHHHCCEEEECCCCCHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: SO42- [Periplasm]; H2O; ATP; S2O32- [Periplasm] [C]

Specific reaction: SO42- [Periplasm] + H2O + ATP = SO42- [Cytoplasm] + phosphate + ADP ATP + S2O32- [Periplasm] + H2O = ADP + phosphate + S2O32- [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2188959; 9205837; 9278503; 9298646 [H]