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The map label for this gene is 83312582

Identifier: 83312582

GI number: 83312582

Start: 3828780

End: 3830222

Strand: Reverse

Name: 83312582

Synonym: amb3483

Alternate gene names: NA

Gene position: 3830222-3828780 (Counterclockwise)

Preceding gene: 83312583

Following gene: 83312578

Centisome position: 77.11

GC content: 63.13

Gene sequence:

>1443_bases
ATGAAGAAGTCGCTTTTCCTCAATCCGCCCTCGTTCGAAGGTTATGACGGCGGGGCCGGTGCCCGTTTCCAGGCGAAGCG
CGAGATCAAGTCCAACTGGTATCCCACCTGGCTGGCCCAGCCGGCCGCCATGGTTCCCGGCTCCAAGCTGGTGGACGCCC
CGGCCTCGGGCAAGACCCTGGACGACTGCCTGAAGCTGGCCAAGGATTACGAGCTGCTGGTGATCTATGCCGGCGCCGCC
ACCTATGCCTCCGAGTGCAAGGTGGCCGAGGCCTTCAAGGCCGCCAATCCGAACATCATGATCGGCATGGTCGGCGCCCA
TGTGGCGACGGTGCCCGAGCCCTCGCTGCTGGCGTCGGACGCCATCGACTTCGTGGCGCGGCACGAGTTCGACTACACCA
TCGTCGAAGTGGCCGAGGGCAAGCCCTTCGCCGAGATCGACGGCCTGACCTTCCGGGGCGAGGACGGCAAGCCGGTCCAC
ACCAAGGACCGCGCCATGATCCACGACATGGACGCGCTGCCCTTCGTCGGTCCGGTCTATCGCCGCGACCTCAATCCCGA
CGATTACTTCATCGGCTATATCCGCCATCCCTACATGGCGTTCTATACGGGGCGCGGCTGCAAGTCCAAGTGCAGCTTCT
GCCTGTGGCCGCAGACCATCGGCGGCCGCGTCTACCGCGCCCGCAGCGCCAAGAGCGTCATCGAGGAAGTGACCCTGGCC
CGCCAGATGTTCCCCGAGGTGCGCGAGTTCTTCTTCGACGACGACACCTTCACCGACGATCTGGAGCGGGTCGAGGAAAT
CGCCAAGGGCATCGGCCATCTGGGCGTGCCGTGGTCGTGCAACGCCAAGGCCAACATTCCGCGCAAGACCCTGGAAGTGC
TGAAGGCCAACGGCCTGCGCGTTCTGGTGGTTGGCTATGAATCCGGCGTGCAGGAAATCCTGAACAACATCAGGAAGGGC
CTGCGCATCGATATCATCAAGCGCTTCTCCCAGGACTGCCACGAATTGGGCATCGTGGTGCACGGCACCTTCATCCTGGG
TCTGCCGGGCGAGACCCGCGAGACCATCAAGCAGACCCTGGCCTTCGCCAAGGAGGTCAATCCCCGCACCCTGCAGGTTT
CCATGGCGGCGCCCTATCCCGGCACCGAACTGTACGAGCAGGCCATCGCCAACGGCTGGTTCAAGCAGGACAAGGATCTG
GTGATGAAGGAAACCGGCGGCGGCGGCTGGCAGGTGGCAGCGCTGTCCTATCCCGACCTGTCGTCCGAGGAAATCTTCAA
GGGCGTGGCCGAGTTCTACAAGGCCTTCTATTTCCGCCCATCCAAGATCGGCGAGATCGTCTGGGAGATGCTCCACGACT
GGGACATGATGAAGCGTCGCCTGCGTGAAGGCGTCGAGTTTTTCCAGTTCCTCAGGACCCGCAACAAGCCGATGGAATGC
TAG

Upstream 100 bases:

>100_bases
TCTATGTTGTCGCATGTTGCGGACGGACTGTGGTATGGCGTGGCCGGCGATTCGCCGTCCGTGCCGACGGCACACTCGAT
CAGGTTGAAGGCAGTCAGAC

Downstream 100 bases:

>100_bases
CCGCTTTCCCTTAGGACAAGATGGAGAAGGGGGGCCTCAGCCCCCCTTTTTCATGCGCAGCTTATCCCAGTATTCCAGCC
GCTTCCTGATCTCGCGTTCG

Product: Fe-S oxidoreductase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 480; Mature: 480

Protein sequence:

>480_residues
MKKSLFLNPPSFEGYDGGAGARFQAKREIKSNWYPTWLAQPAAMVPGSKLVDAPASGKTLDDCLKLAKDYELLVIYAGAA
TYASECKVAEAFKAANPNIMIGMVGAHVATVPEPSLLASDAIDFVARHEFDYTIVEVAEGKPFAEIDGLTFRGEDGKPVH
TKDRAMIHDMDALPFVGPVYRRDLNPDDYFIGYIRHPYMAFYTGRGCKSKCSFCLWPQTIGGRVYRARSAKSVIEEVTLA
RQMFPEVREFFFDDDTFTDDLERVEEIAKGIGHLGVPWSCNAKANIPRKTLEVLKANGLRVLVVGYESGVQEILNNIRKG
LRIDIIKRFSQDCHELGIVVHGTFILGLPGETRETIKQTLAFAKEVNPRTLQVSMAAPYPGTELYEQAIANGWFKQDKDL
VMKETGGGGWQVAALSYPDLSSEEIFKGVAEFYKAFYFRPSKIGEIVWEMLHDWDMMKRRLREGVEFFQFLRTRNKPMEC

Sequences:

>Translated_480_residues
MKKSLFLNPPSFEGYDGGAGARFQAKREIKSNWYPTWLAQPAAMVPGSKLVDAPASGKTLDDCLKLAKDYELLVIYAGAA
TYASECKVAEAFKAANPNIMIGMVGAHVATVPEPSLLASDAIDFVARHEFDYTIVEVAEGKPFAEIDGLTFRGEDGKPVH
TKDRAMIHDMDALPFVGPVYRRDLNPDDYFIGYIRHPYMAFYTGRGCKSKCSFCLWPQTIGGRVYRARSAKSVIEEVTLA
RQMFPEVREFFFDDDTFTDDLERVEEIAKGIGHLGVPWSCNAKANIPRKTLEVLKANGLRVLVVGYESGVQEILNNIRKG
LRIDIIKRFSQDCHELGIVVHGTFILGLPGETRETIKQTLAFAKEVNPRTLQVSMAAPYPGTELYEQAIANGWFKQDKDL
VMKETGGGGWQVAALSYPDLSSEEIFKGVAEFYKAFYFRPSKIGEIVWEMLHDWDMMKRRLREGVEFFQFLRTRNKPMEC
>Mature_480_residues
MKKSLFLNPPSFEGYDGGAGARFQAKREIKSNWYPTWLAQPAAMVPGSKLVDAPASGKTLDDCLKLAKDYELLVIYAGAA
TYASECKVAEAFKAANPNIMIGMVGAHVATVPEPSLLASDAIDFVARHEFDYTIVEVAEGKPFAEIDGLTFRGEDGKPVH
TKDRAMIHDMDALPFVGPVYRRDLNPDDYFIGYIRHPYMAFYTGRGCKSKCSFCLWPQTIGGRVYRARSAKSVIEEVTLA
RQMFPEVREFFFDDDTFTDDLERVEEIAKGIGHLGVPWSCNAKANIPRKTLEVLKANGLRVLVVGYESGVQEILNNIRKG
LRIDIIKRFSQDCHELGIVVHGTFILGLPGETRETIKQTLAFAKEVNPRTLQVSMAAPYPGTELYEQAIANGWFKQDKDL
VMKETGGGGWQVAALSYPDLSSEEIFKGVAEFYKAFYFRPSKIGEIVWEMLHDWDMMKRRLREGVEFFQFLRTRNKPMEC

Specific function: Unknown

COG id: COG1032

COG function: function code C; Fe-S oxidoreductase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 B12-binding domain [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006158
- InterPro:   IPR006638
- InterPro:   IPR005839
- InterPro:   IPR007197 [H]

Pfam domain/function: PF02310 B12-binding; PF04055 Radical_SAM [H]

EC number: NA

Molecular weight: Translated: 53972; Mature: 53972

Theoretical pI: Translated: 6.61; Mature: 6.61

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKSLFLNPPSFEGYDGGAGARFQAKREIKSNWYPTWLAQPAAMVPGSKLVDAPASGKTL
CCCCEECCCCCCCCCCCCCCCCHHHHHHHHCCCCCHHHCCCCCCCCCCCEECCCCCCCCH
DDCLKLAKDYELLVIYAGAATYASECKVAEAFKAANPNIMIGMVGAHVATVPEPSLLASD
HHHHHHHCCCEEEEEEECCHHHHHHHHHHHHHHCCCCCEEEEEECCEEECCCCCHHHHHH
AIDFVARHEFDYTIVEVAEGKPFAEIDGLTFRGEDGKPVHTKDRAMIHDMDALPFVGPVY
HHHHHHHCCCCEEEEEEECCCCCEEECCEEEECCCCCCCCCCCCHHHHCCCCCCCCCHHH
RRDLNPDDYFIGYIRHPYMAFYTGRGCKSKCSFCLWPQTIGGRVYRARSAKSVIEEVTLA
HCCCCCCCEEEEEECCCEEEEEECCCCHHCCCEEECCHHHCCEEEEHHHHHHHHHHHHHH
RQMFPEVREFFFDDDTFTDDLERVEEIAKGIGHLGVPWSCNAKANIPRKTLEVLKANGLR
HHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHCCCCE
VLVVGYESGVQEILNNIRKGLRIDIIKRFSQDCHELGIVVHGTFILGLPGETRETIKQTL
EEEEECHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCEEEEEEEEEECCCCHHHHHHHHH
AFAKEVNPRTLQVSMAAPYPGTELYEQAIANGWFKQDKDLVMKETGGGGWQVAALSYPDL
HHHHCCCCCEEEEEEECCCCCHHHHHHHHHCCCCCCCCCEEEEECCCCCEEEEEEECCCC
SSEEIFKGVAEFYKAFYFRPSKIGEIVWEMLHDWDMMKRRLREGVEFFQFLRTRNKPMEC
CHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MKKSLFLNPPSFEGYDGGAGARFQAKREIKSNWYPTWLAQPAAMVPGSKLVDAPASGKTL
CCCCEECCCCCCCCCCCCCCCCHHHHHHHHCCCCCHHHCCCCCCCCCCCEECCCCCCCCH
DDCLKLAKDYELLVIYAGAATYASECKVAEAFKAANPNIMIGMVGAHVATVPEPSLLASD
HHHHHHHCCCEEEEEEECCHHHHHHHHHHHHHHCCCCCEEEEEECCEEECCCCCHHHHHH
AIDFVARHEFDYTIVEVAEGKPFAEIDGLTFRGEDGKPVHTKDRAMIHDMDALPFVGPVY
HHHHHHHCCCCEEEEEEECCCCCEEECCEEEECCCCCCCCCCCCHHHHCCCCCCCCCHHH
RRDLNPDDYFIGYIRHPYMAFYTGRGCKSKCSFCLWPQTIGGRVYRARSAKSVIEEVTLA
HCCCCCCCEEEEEECCCEEEEEECCCCHHCCCEEECCHHHCCEEEEHHHHHHHHHHHHHH
RQMFPEVREFFFDDDTFTDDLERVEEIAKGIGHLGVPWSCNAKANIPRKTLEVLKANGLR
HHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHCCCCE
VLVVGYESGVQEILNNIRKGLRIDIIKRFSQDCHELGIVVHGTFILGLPGETRETIKQTL
EEEEECHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCEEEEEEEEEECCCCHHHHHHHHH
AFAKEVNPRTLQVSMAAPYPGTELYEQAIANGWFKQDKDLVMKETGGGGWQVAALSYPDL
HHHHCCCCCEEEEEEECCCCCHHHHHHHHHCCCCCCCCCEEEEECCCCCEEEEEEECCCC
SSEEIFKGVAEFYKAFYFRPSKIGEIVWEMLHDWDMMKRRLREGVEFFQFLRTRNKPMEC
CHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9679194 [H]