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The map label for this gene is leuA [H]

Identifier: 83312567

GI number: 83312567

Start: 3809338

End: 3810999

Strand: Reverse

Name: leuA [H]

Synonym: amb3468

Alternate gene names: 83312567

Gene position: 3810999-3809338 (Counterclockwise)

Preceding gene: 83312570

Following gene: 83312566

Centisome position: 76.72

GC content: 66.91

Gene sequence:

>1662_bases
ATGATGCCTGCCGCCAGCACCAAGTACCGCGCCTATCCCGCCATCCGCCTCGCCCAGCGCCGCTGGCCCGATGCCACCAT
CACCCGCCCGCCGGTATGGTGCAGCGTCGATTTGCGCGACGGCAATCAGGCGCTGATCGATCCCATGGGCTCGGAGCGCA
AGATGGCCCTGTGGCAGACCCTGATCGGCATGGGCTTCAAGGAGATCGAGGTGGGCTTTCCCGCCGCGTCGCAGACCGAT
TTCGACTTCGTGCGCCTGCTGATCGAGGGCGGGCATATTCCCGAGGACGTCACCATCCAGGTGCTGGTCCAGTCGCGCGA
CGAGTTGATCCGCCGTACCTTCGAGGCGCTGGCCGGTGCCAGGCGGGTGATCGTCCACATGTACAACTCCACCTCCGAGG
CGCAGCGGCGCATCGTGTTCGGCATCGACCGCCAGGGCTGCATCGACCTCGCCCTGTCCGGGGTCAGGCTGGTGCGCGAG
CTGTCCGCCGCCCGGCCGGAAGGCGAGGTGGTGTTCCAGTATTCGCCCGAAAGCTTCACCGGCACCGAGCCGGACTTCGC
CATGGACATCGTCGAGAAGGTGGCCGAGGCCTGGGGCGCCACGCCCGAGCGCAAGATGATCTGCAACCTGCCGGCCACGG
TGGAGATGTCGACCCCCAACATCTACGCCGACGTCATCGAATGGTTCTGTGCCCATGTGAAGAACCGCGACTCCCTCATC
GTTTCGGTCCATCCCCATAATGACCGGGGCACCGGCGTGGCGGCGGCGGAACTGGCGGTGATGGCCGGCGCCGACCGGGT
GGAGGGCACCCTGTTCGGCAATGGCGAGCGCACCGGCAATGTGGACGTGGTCACCCTGGCGCTCAACCTCTATACCCAGG
GCGTCGATCCCGCCCTTGACCTGTCGGACATCGACACGGTGCGGCGCACGGCGGAACAATGCACGGGGCTGCCCGTCCAT
CCCCGCCACCCCTATGCGGGCGAACTGGTCTATACCGCCTTTTCCGGCTCGCACCAGGACGCCATCAAGAAGGGCTTCTC
CGCCCGCAAGAAGGCCAACGACCCGGTGTGGGAGGTTCCGTACCTTCCCATCGATCCGGCCGATGTGGGGCGCAGCTACG
AGGCGGTGATCCGCATCAACAGCCAGTCGGGCAAGGGCGGTATCGCCGCCGTGCTGGAGCGCGACCACGGGCTGGAGATC
CCCCGGCCGCTGCAGATCGACTTCGCCCGGGTGGTGCAGGCCATGGCAGACAAGGTGGGCCGCGAGCTGGATTCGGGTGA
GATCATGGCCGCCTTTTCCCAGACCTATCTCAAGGACTGGCCGCTGGAACTGGTGGATTACGAGACCCATTCCGAAGGAC
GCGGCGCCACGCGGATGCTGAGCGCCACCATCCGCGACAAGGGCGTGGAAAAGCGCATCGAAGGCCGGGGTAACGGCCCG
CTTGACGCCTTCATCCATGCCCTGGAAACCGGCCTCGGCCGCAAGGTCAAGGTGCGGGACTACCACGAGCACGCCCTGGG
CTCGGGCTCGGAGGCTCAGGCTGCCTGCTATGTGGAAATCGCCGGCGAGGGCGGAGGCTCGGTCCACGGCGCCGCCCTGG
ATTCCTCCATCACCATGGCCTCGCTGAAGGCGGTGGTCAGCGCGCTGAACCGGGCGGCATGA

Upstream 100 bases:

>100_bases
TGCGGTCGCCGGGGCTAAGGTCATTCGCGGCCGCCCGGCGGCCGGAAAGACCGGGTCGCGCTCCCCATATCAGCAGTCAG
ACCATAAGGAGGACCAGGCC

Downstream 100 bases:

>100_bases
AAATACGGCGGGGTGGTGTGAAATAATCCGCCCCGCCGATTTATGTAAGACGTAAATGGATTTTACAAATTATTCATATA
CCAAAAGTATGTAAAATATG

Product: 2-isopropylmalate synthase

Products: NA

Alternate protein names: Alpha-IPM synthase; Alpha-isopropylmalate synthase [H]

Number of amino acids: Translated: 553; Mature: 553

Protein sequence:

>553_residues
MMPAASTKYRAYPAIRLAQRRWPDATITRPPVWCSVDLRDGNQALIDPMGSERKMALWQTLIGMGFKEIEVGFPAASQTD
FDFVRLLIEGGHIPEDVTIQVLVQSRDELIRRTFEALAGARRVIVHMYNSTSEAQRRIVFGIDRQGCIDLALSGVRLVRE
LSAARPEGEVVFQYSPESFTGTEPDFAMDIVEKVAEAWGATPERKMICNLPATVEMSTPNIYADVIEWFCAHVKNRDSLI
VSVHPHNDRGTGVAAAELAVMAGADRVEGTLFGNGERTGNVDVVTLALNLYTQGVDPALDLSDIDTVRRTAEQCTGLPVH
PRHPYAGELVYTAFSGSHQDAIKKGFSARKKANDPVWEVPYLPIDPADVGRSYEAVIRINSQSGKGGIAAVLERDHGLEI
PRPLQIDFARVVQAMADKVGRELDSGEIMAAFSQTYLKDWPLELVDYETHSEGRGATRMLSATIRDKGVEKRIEGRGNGP
LDAFIHALETGLGRKVKVRDYHEHALGSGSEAQAACYVEIAGEGGGSVHGAALDSSITMASLKAVVSALNRAA

Sequences:

>Translated_553_residues
MMPAASTKYRAYPAIRLAQRRWPDATITRPPVWCSVDLRDGNQALIDPMGSERKMALWQTLIGMGFKEIEVGFPAASQTD
FDFVRLLIEGGHIPEDVTIQVLVQSRDELIRRTFEALAGARRVIVHMYNSTSEAQRRIVFGIDRQGCIDLALSGVRLVRE
LSAARPEGEVVFQYSPESFTGTEPDFAMDIVEKVAEAWGATPERKMICNLPATVEMSTPNIYADVIEWFCAHVKNRDSLI
VSVHPHNDRGTGVAAAELAVMAGADRVEGTLFGNGERTGNVDVVTLALNLYTQGVDPALDLSDIDTVRRTAEQCTGLPVH
PRHPYAGELVYTAFSGSHQDAIKKGFSARKKANDPVWEVPYLPIDPADVGRSYEAVIRINSQSGKGGIAAVLERDHGLEI
PRPLQIDFARVVQAMADKVGRELDSGEIMAAFSQTYLKDWPLELVDYETHSEGRGATRMLSATIRDKGVEKRIEGRGNGP
LDAFIHALETGLGRKVKVRDYHEHALGSGSEAQAACYVEIAGEGGGSVHGAALDSSITMASLKAVVSALNRAA
>Mature_553_residues
MMPAASTKYRAYPAIRLAQRRWPDATITRPPVWCSVDLRDGNQALIDPMGSERKMALWQTLIGMGFKEIEVGFPAASQTD
FDFVRLLIEGGHIPEDVTIQVLVQSRDELIRRTFEALAGARRVIVHMYNSTSEAQRRIVFGIDRQGCIDLALSGVRLVRE
LSAARPEGEVVFQYSPESFTGTEPDFAMDIVEKVAEAWGATPERKMICNLPATVEMSTPNIYADVIEWFCAHVKNRDSLI
VSVHPHNDRGTGVAAAELAVMAGADRVEGTLFGNGERTGNVDVVTLALNLYTQGVDPALDLSDIDTVRRTAEQCTGLPVH
PRHPYAGELVYTAFSGSHQDAIKKGFSARKKANDPVWEVPYLPIDPADVGRSYEAVIRINSQSGKGGIAAVLERDHGLEI
PRPLQIDFARVVQAMADKVGRELDSGEIMAAFSQTYLKDWPLELVDYETHSEGRGATRMLSATIRDKGVEKRIEGRGNGP
LDAFIHALETGLGRKVKVRDYHEHALGSGSEAQAACYVEIAGEGGGSVHGAALDSSITMASLKAVVSALNRAA

Specific function: Catalyzes the condensation of the acetyl group of acetyl-CoA with 3-methyl-2-oxobutanoate (2-oxoisovalerate) to form 3-carboxy-3-hydroxy-4-methylpentanoate (2-isopropylmalate) [H]

COG id: COG0119

COG function: function code E; Isopropylmalate/homocitrate/citramalate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the alpha-IPM synthase/homocitrate synthase family. LeuA type 2 subfamily [H]

Homologues:

Organism=Escherichia coli, GI1786261, Length=519, Percent_Identity=26.2042389210019, Blast_Score=112, Evalue=6e-26,
Organism=Saccharomyces cerevisiae, GI6324682, Length=569, Percent_Identity=48.506151142355, Blast_Score=547, Evalue=1e-156,
Organism=Saccharomyces cerevisiae, GI6324225, Length=567, Percent_Identity=47.7954144620811, Blast_Score=540, Evalue=1e-154,

Paralogues:

None

Copy number: 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013709
- InterPro:   IPR002034
- InterPro:   IPR013785
- InterPro:   IPR005668
- InterPro:   IPR000891 [H]

Pfam domain/function: PF00682 HMGL-like; PF08502 LeuA_dimer [H]

EC number: =2.3.3.13 [H]

Molecular weight: Translated: 60305; Mature: 60305

Theoretical pI: Translated: 5.64; Mature: 5.64

Prosite motif: PS00815 AIPM_HOMOCIT_SYNTH_1 ; PS00816 AIPM_HOMOCIT_SYNTH_2 ; PS50991 PYR_CT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMPAASTKYRAYPAIRLAQRRWPDATITRPPVWCSVDLRDGNQALIDPMGSERKMALWQT
CCCCCCCCCCCCHHHHHHHHCCCCCCCCCCCEEEEEEECCCCEEEECCCCCCHHHHHHHH
LIGMGFKEIEVGFPAASQTDFDFVRLLIEGGHIPEDVTIQVLVQSRDELIRRTFEALAGA
HHHCCCEEEEECCCCCCCCCHHHHHHHHHCCCCCCCCEEEEEHHHHHHHHHHHHHHHHCC
RRVIVHMYNSTSEAQRRIVFGIDRQGCIDLALSGVRLVRELSAARPEGEVVFQYSPESFT
CEEEEEEECCCHHHCCEEEEECCCCCHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCC
GTEPDFAMDIVEKVAEAWGATPERKMICNLPATVEMSTPNIYADVIEWFCAHVKNRDSLI
CCCCHHHHHHHHHHHHHHCCCCCCCEEECCCCEEEECCCCHHHHHHHHHHHHHCCCCCEE
VSVHPHNDRGTGVAAAELAVMAGADRVEGTLFGNGERTGNVDVVTLALNLYTQGVDPALD
EEEECCCCCCCCHHHHHHHHHCCCCCCCEEEEECCCCCCCEEEEEEEEEHHHCCCCCCCC
LSDIDTVRRTAEQCTGLPVHPRHPYAGELVYTAFSGSHQDAIKKGFSARKKANDPVWEVP
HHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEECCCHHHHHHHCHHHHHCCCCCCEECC
YLPIDPADVGRSYEAVIRINSQSGKGGIAAVLERDHGLEIPRPLQIDFARVVQAMADKVG
CCCCCHHHHCCCEEEEEEEECCCCCCCEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHC
RELDSGEIMAAFSQTYLKDWPLELVDYETHSEGRGATRMLSATIRDKGVEKRIEGRGNGP
CCCCCCHHHHHHHHHHHHCCCEEEEECCCCCCCCCHHHHHHHHHHHCCHHHHHCCCCCCC
LDAFIHALETGLGRKVKVRDYHEHALGSGSEAQAACYVEIAGEGGGSVHGAALDSSITMA
HHHHHHHHHHCCCCEEEEEEHHHHHCCCCCCCCEEEEEEEECCCCCCEECHHCCCHHHHH
SLKAVVSALNRAA
HHHHHHHHHHHCC
>Mature Secondary Structure
MMPAASTKYRAYPAIRLAQRRWPDATITRPPVWCSVDLRDGNQALIDPMGSERKMALWQT
CCCCCCCCCCCCHHHHHHHHCCCCCCCCCCCEEEEEEECCCCEEEECCCCCCHHHHHHHH
LIGMGFKEIEVGFPAASQTDFDFVRLLIEGGHIPEDVTIQVLVQSRDELIRRTFEALAGA
HHHCCCEEEEECCCCCCCCCHHHHHHHHHCCCCCCCCEEEEEHHHHHHHHHHHHHHHHCC
RRVIVHMYNSTSEAQRRIVFGIDRQGCIDLALSGVRLVRELSAARPEGEVVFQYSPESFT
CEEEEEEECCCHHHCCEEEEECCCCCHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCC
GTEPDFAMDIVEKVAEAWGATPERKMICNLPATVEMSTPNIYADVIEWFCAHVKNRDSLI
CCCCHHHHHHHHHHHHHHCCCCCCCEEECCCCEEEECCCCHHHHHHHHHHHHHCCCCCEE
VSVHPHNDRGTGVAAAELAVMAGADRVEGTLFGNGERTGNVDVVTLALNLYTQGVDPALD
EEEECCCCCCCCHHHHHHHHHCCCCCCCEEEEECCCCCCCEEEEEEEEEHHHCCCCCCCC
LSDIDTVRRTAEQCTGLPVHPRHPYAGELVYTAFSGSHQDAIKKGFSARKKANDPVWEVP
HHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEECCCHHHHHHHCHHHHHCCCCCCEECC
YLPIDPADVGRSYEAVIRINSQSGKGGIAAVLERDHGLEIPRPLQIDFARVVQAMADKVG
CCCCCHHHHCCCEEEEEEEECCCCCCCEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHC
RELDSGEIMAAFSQTYLKDWPLELVDYETHSEGRGATRMLSATIRDKGVEKRIEGRGNGP
CCCCCCHHHHHHHHHHHHCCCEEEEECCCCCCCCCHHHHHHHHHHHCCHHHHHCCCCCCC
LDAFIHALETGLGRKVKVRDYHEHALGSGSEAQAACYVEIAGEGGGSVHGAALDSSITMA
HHHHHHHHHHCCCCEEEEEEHHHHHCCCCCCCCEEEEEEEECCCCCCEECHHCCCHHHHH
SLKAVVSALNRAA
HHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA