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The map label for this gene is nifS1 [H]

Identifier: 83312128

GI number: 83312128

Start: 3271350

End: 3272483

Strand: Reverse

Name: nifS1 [H]

Synonym: amb3029

Alternate gene names: 83312128

Gene position: 3272483-3271350 (Counterclockwise)

Preceding gene: 83312129

Following gene: 83312127

Centisome position: 65.88

GC content: 70.63

Gene sequence:

>1134_bases
GTGAGCAGGGCCGTCTACCTCGATTACAACTCCGGCGCCCCCGTGCGCCCCGAGGTGGCGGCCGCCATGGTGGAGTCCCT
GGCCGAGCCGGGCAACCCGTCCTCGGTTCATGCTTTCGGTCGTGCGGCCCGCGCCCGGGTCGAGGCGGCCAGGCGGCAAT
TGGCCGGGCTGGTGGGCGCCGATCCCGCCGGCATCGTCTTCACCTCGGGCGGCACCGAGGCCAATGCCCTGGCGCTTCGC
GGCTGTGGCCGGTCCCGCCAGCTGGTGTCGGCCGTCGAGCATCCCTCTGTCCTTGATGCCGCCGCCGAGGCCGAGCGGAT
TCCCGTCACCCGGGACGGAATCGTCGATCTGGGCGCCCTGGAAAGCCTGCTGGCGGCCAATACCCGCCCGGCGCTGGTGT
CGCTGATGCTGGCCAACAACGAGACCGGAATCGTCCAGCCGGTGGCCGAAGCGGCTCGCATCATTCATGCGCATGGCGCA
TGGCTTCATTGCGATGCCGCCCAGGCCATGGGGCGAATCCCGGTCTCCCTTCGGGACTTGGGGGCGGATATCCTGACGGT
TTCCGGCCATAAGATGGGTGGGCCCGCGGGGGCGGGAGCCTTGATCCTGGCGGAATCCGGCCGGACACTGGCACCGATCT
TGCTGGGCGGAGGCCAGGAACGCCGCCGCCGGGCCGGAACGGAAAACGTGGCCGGCATCGTCGGCCTGGGGGTCGCCGCC
CAACTGGCGGGGGATGATTTGGCTGGCGCCGACACCACATGTGGTGTGGGTGCCTTGCGAGACCGTCTGGAACAAGGCGC
CCTGCGTCTGGTTCCCGACGCAGCGGTGATCGGGGACGGCGCGCCCCGTCTGCCCAACACCACCTGCCTGGTTCTGCCGG
GCGTGGAGGGGCGGACGCAGGTGATGGCGCTGGACCTGGCGGGCGTGGCGGTCAGCGCCGGCGCGGCCTGTTCCTCGGGC
AAGGTTGGTCCCAGTCACGTGCTGGCGGCCATGGGCCTGGAGGAGCGGGTCAAGGGATCGGCCATCCGGGTCAGCCTGGG
CTGGTCCTCGCGCCCGGAGGATGTGGATGCGTTCCTGGCGGCCTGGGTCGGTCTGGCCCGCCACAAGGGATTGAAGGTTT
CTGAGGCCGCCTGA

Upstream 100 bases:

>100_bases
GCGAACGCCGCATCCTGGGCAGCTCGGGCATGTTCCACGGCACCGCCGCTCCCTCTGGAGCCGTGGCGGCGCAATAAGGG
TCGGGAAAGGAAGGAACAGC

Downstream 100 bases:

>100_bases
GGCGGCCTGAATGACAAACGAAGATGTAAGGGATCGATGCAAATGACAGCTCTCGGCAAGACGACCCGCCCCAGCCTGCC
CCGCGCCCCGGGGGCGCCGG

Product: cysteine sulfinate desulfinase/cysteine desulfurase

Products: NA

Alternate protein names: Nitrogenase metalloclusters biosynthesis protein nifS1 [H]

Number of amino acids: Translated: 377; Mature: 376

Protein sequence:

>377_residues
MSRAVYLDYNSGAPVRPEVAAAMVESLAEPGNPSSVHAFGRAARARVEAARRQLAGLVGADPAGIVFTSGGTEANALALR
GCGRSRQLVSAVEHPSVLDAAAEAERIPVTRDGIVDLGALESLLAANTRPALVSLMLANNETGIVQPVAEAARIIHAHGA
WLHCDAAQAMGRIPVSLRDLGADILTVSGHKMGGPAGAGALILAESGRTLAPILLGGGQERRRRAGTENVAGIVGLGVAA
QLAGDDLAGADTTCGVGALRDRLEQGALRLVPDAAVIGDGAPRLPNTTCLVLPGVEGRTQVMALDLAGVAVSAGAACSSG
KVGPSHVLAAMGLEERVKGSAIRVSLGWSSRPEDVDAFLAAWVGLARHKGLKVSEAA

Sequences:

>Translated_377_residues
MSRAVYLDYNSGAPVRPEVAAAMVESLAEPGNPSSVHAFGRAARARVEAARRQLAGLVGADPAGIVFTSGGTEANALALR
GCGRSRQLVSAVEHPSVLDAAAEAERIPVTRDGIVDLGALESLLAANTRPALVSLMLANNETGIVQPVAEAARIIHAHGA
WLHCDAAQAMGRIPVSLRDLGADILTVSGHKMGGPAGAGALILAESGRTLAPILLGGGQERRRRAGTENVAGIVGLGVAA
QLAGDDLAGADTTCGVGALRDRLEQGALRLVPDAAVIGDGAPRLPNTTCLVLPGVEGRTQVMALDLAGVAVSAGAACSSG
KVGPSHVLAAMGLEERVKGSAIRVSLGWSSRPEDVDAFLAAWVGLARHKGLKVSEAA
>Mature_376_residues
SRAVYLDYNSGAPVRPEVAAAMVESLAEPGNPSSVHAFGRAARARVEAARRQLAGLVGADPAGIVFTSGGTEANALALRG
CGRSRQLVSAVEHPSVLDAAAEAERIPVTRDGIVDLGALESLLAANTRPALVSLMLANNETGIVQPVAEAARIIHAHGAW
LHCDAAQAMGRIPVSLRDLGADILTVSGHKMGGPAGAGALILAESGRTLAPILLGGGQERRRRAGTENVAGIVGLGVAAQ
LAGDDLAGADTTCGVGALRDRLEQGALRLVPDAAVIGDGAPRLPNTTCLVLPGVEGRTQVMALDLAGVAVSAGAACSSGK
VGPSHVLAAMGLEERVKGSAIRVSLGWSSRPEDVDAFLAAWVGLARHKGLKVSEAA

Specific function: Catalyzes the removal of elemental sulfur atoms from cysteine to produce alanine. Seems to participate in the biosynthesis of the nitrogenase metalloclusters by providing the inorganic sulfur required for the Fe-S core formation [H]

COG id: COG1104

COG function: function code E; Cysteine sulfinate desulfinase/cysteine desulfurase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-V pyridoxal-phosphate-dependent aminotransferase family. NifS/IscS subfamily [H]

Homologues:

Organism=Homo sapiens, GI32307132, Length=370, Percent_Identity=39.1891891891892, Blast_Score=241, Evalue=5e-64,
Organism=Homo sapiens, GI156713448, Length=407, Percent_Identity=35.8722358722359, Blast_Score=189, Evalue=3e-48,
Organism=Escherichia coli, GI48994898, Length=374, Percent_Identity=39.0374331550802, Blast_Score=230, Evalue=1e-61,
Organism=Escherichia coli, GI1787970, Length=322, Percent_Identity=28.5714285714286, Blast_Score=91, Evalue=1e-19,
Organism=Escherichia coli, GI1789175, Length=397, Percent_Identity=29.2191435768262, Blast_Score=83, Evalue=2e-17,
Organism=Caenorhabditis elegans, GI25143064, Length=370, Percent_Identity=36.2162162162162, Blast_Score=215, Evalue=3e-56,
Organism=Caenorhabditis elegans, GI17533177, Length=330, Percent_Identity=34.5454545454545, Blast_Score=164, Evalue=6e-41,
Organism=Saccharomyces cerevisiae, GI6319831, Length=378, Percent_Identity=34.6560846560847, Blast_Score=206, Evalue=6e-54,
Organism=Drosophila melanogaster, GI20129463, Length=370, Percent_Identity=38.3783783783784, Blast_Score=218, Evalue=6e-57,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000192
- InterPro:   IPR020578
- InterPro:   IPR017772
- InterPro:   IPR016454
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF00266 Aminotran_5 [H]

EC number: =2.8.1.7 [H]

Molecular weight: Translated: 38123; Mature: 37992

Theoretical pI: Translated: 6.94; Mature: 6.94

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSRAVYLDYNSGAPVRPEVAAAMVESLAEPGNPSSVHAFGRAARARVEAARRQLAGLVGA
CCCEEEEECCCCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCC
DPAGIVFTSGGTEANALALRGCGRSRQLVSAVEHPSVLDAAAEAERIPVTRDGIVDLGAL
CCCCEEEECCCCCCCEEEEECCCCHHHHHHHHCCCHHHHHHHHHHCCCCCCCCCCCHHHH
ESLLAANTRPALVSLMLANNETGIVQPVAEAARIIHAHGAWLHCDAAQAMGRIPVSLRDL
HHHHHCCCCCCEEEEEEECCCCCCCHHHHHHHHHHHHCCCEEEECHHHHHCCCCCHHHHC
GADILTVSGHKMGGPAGAGALILAESGRTLAPILLGGGQERRRRAGTENVAGIVGLGVAA
CCCEEEECCCCCCCCCCCCEEEEECCCCEEEEEEECCCHHHHHHCCCCCCHHHHHHHHHH
QLAGDDLAGADTTCGVGALRDRLEQGALRLVPDAAVIGDGAPRLPNTTCLVLPGVEGRTQ
HHCCCCCCCCCCCCCHHHHHHHHHCCCEEECCCHHEECCCCCCCCCCEEEEECCCCCCCE
VMALDLAGVAVSAGAACSSGKVGPSHVLAAMGLEERVKGSAIRVSLGWSSRPEDVDAFLA
EEEEEECCHHHCCCCCCCCCCCCHHHHHHHCCHHHHCCCCEEEEEECCCCCCHHHHHHHH
AWVGLARHKGLKVSEAA
HHHHHHHHCCCCCCCCC
>Mature Secondary Structure 
SRAVYLDYNSGAPVRPEVAAAMVESLAEPGNPSSVHAFGRAARARVEAARRQLAGLVGA
CCEEEEECCCCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCC
DPAGIVFTSGGTEANALALRGCGRSRQLVSAVEHPSVLDAAAEAERIPVTRDGIVDLGAL
CCCCEEEECCCCCCCEEEEECCCCHHHHHHHHCCCHHHHHHHHHHCCCCCCCCCCCHHHH
ESLLAANTRPALVSLMLANNETGIVQPVAEAARIIHAHGAWLHCDAAQAMGRIPVSLRDL
HHHHHCCCCCCEEEEEEECCCCCCCHHHHHHHHHHHHCCCEEEECHHHHHCCCCCHHHHC
GADILTVSGHKMGGPAGAGALILAESGRTLAPILLGGGQERRRRAGTENVAGIVGLGVAA
CCCEEEECCCCCCCCCCCCEEEEECCCCEEEEEEECCCHHHHHHCCCCCCHHHHHHHHHH
QLAGDDLAGADTTCGVGALRDRLEQGALRLVPDAAVIGDGAPRLPNTTCLVLPGVEGRTQ
HHCCCCCCCCCCCCCHHHHHHHHHCCCEEECCCHHEECCCCCCCCCCEEEEECCCCCCCE
VMALDLAGVAVSAGAACSSGKVGPSHVLAAMGLEERVKGSAIRVSLGWSSRPEDVDAFLA
EEEEEECCHHHCCCCCCCCCCCCHHHHHHHCCHHHHCCCCEEEEEECCCCCCHHHHHHHH
AWVGLARHKGLKVSEAA
HHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7568132 [H]