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The map label for this gene is iscS [H]

Identifier: 83312127

GI number: 83312127

Start: 3270051

End: 3271313

Strand: Reverse

Name: iscS [H]

Synonym: amb3028

Alternate gene names: 83312127

Gene position: 3271313-3270051 (Counterclockwise)

Preceding gene: 83312128

Following gene: 83312126

Centisome position: 65.86

GC content: 64.77

Gene sequence:

>1263_bases
ATGCAAATGACAGCTCTCGGCAAGACGACCCGCCCCAGCCTGCCCCGCGCCCCGGGGGCGCCGGTCTATCTGGACTATCA
GGCGACGACGCCTTGCGATCCGCGGGTGGTCGAGGTCATGCTGCCCTGGTTCACCGAGAAATTCGGCAACCCCCATTCGC
GCAACCACCGTTACGGCTGGGAAGCCGAGGAAGCGGTGGAGAAGGCCCGCGAGCAGATCGCCGACATCATCGGCGCCGAT
TCCAAGGAGGTGATCTTCACCTCGGGCGCCACCGAGTCCAACAATCTGGCCATCAAGGGCGTCGCCCATTTCTACAAGGA
AAAGAAGAACCACATCGTCACCGTGGTGACCGAGCACAAATGCGTGCTCGACACCTGCCGCTACCTGGAACAGGAAGGCT
TTGCGGTGACCTATTTGCCGGTCAAGGCCGACGGGCTGATCGATCTGGCCGAGCTGGAGGCCGCCATCACCGACCAGACG
GTCATCGTCTCGGTCATGGCGGTCAACAACGAGATCGGCGTGATCCAGCCCCTGGCCGAGATCGGCGCCCTGTGCCGCAA
GAAGGGTGCCTTCTTTCATACCGACTGTGCCCAAGCGGTAGGCAAGATCCCGCTGGACGTAAACGCCATGAACATCGACC
TGATGAGCATCTCGGGTCACAAGCTGTATGGCCCCAAGGGCATCGGTGCCCTGTACGTCCGCCGCCGCCCGCGCGTCCGG
CTGGTGCCGCTGATCACCGGCGGCGGCCAGGAGCGCGGCATGCGCTCGGGCACCCTGGCCACCCCGCTCTGTGTGGGCTT
CGGCGAGGCCTGCGCCATCGCCAAGGCCGAGATGGGCGCCGAGAACGAGCGCCTGATGGGCCTGCGCAACCGCCTCCTGG
GCGGGTTGAGGCAGCGCCTGCCGGAAATCTACGTCAACGGCGATCTGGATCACCGCATCCCCGGCAACCTCAACATCTCG
TTCGCCTTCGTCGAGGGCGAGGGGCTGATGATGGGGGTCAAGGAACTGGCGGTGTCGTCGGGGTCGGCCTGCACCTCGGC
CTCGCTGGAGCCCTCCTACGTGCTGCGCGCCCTGGGCGTCGACGTGGAGATGGCTCATACGTCGTTGCGCTTCGGCCTGG
GCCGCTTCACCACCGACGAGGACATCGAATTCGCCATCGACCATATCACCGAGGCGGTCGAGCACTTGCGGGCCATGAGC
CCGCTGTGGGACATGCATCTGGAAGGCGTCGATCTCAAGTCCATCGAATGGGCTGAGCATTGA

Upstream 100 bases:

>100_bases
GTTCCTGGCGGCCTGGGTCGGTCTGGCCCGCCACAAGGGATTGAAGGTTTCTGAGGCCGCCTGAGGCGGCCTGAATGACA
AACGAAGATGTAAGGGATCG

Downstream 100 bases:

>100_bases
GGAAAAGGATGGGCATTTCGGTACCGAAATGCCTTAGAATGATTCCGAGCTAAGGCTCGGCCGGACTTTTGGAGGACGTT
ATGGCTTACAGCGACAAGGT

Product: cysteine sulfinate desulfinase/cysteine desulfurase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 420; Mature: 420

Protein sequence:

>420_residues
MQMTALGKTTRPSLPRAPGAPVYLDYQATTPCDPRVVEVMLPWFTEKFGNPHSRNHRYGWEAEEAVEKAREQIADIIGAD
SKEVIFTSGATESNNLAIKGVAHFYKEKKNHIVTVVTEHKCVLDTCRYLEQEGFAVTYLPVKADGLIDLAELEAAITDQT
VIVSVMAVNNEIGVIQPLAEIGALCRKKGAFFHTDCAQAVGKIPLDVNAMNIDLMSISGHKLYGPKGIGALYVRRRPRVR
LVPLITGGGQERGMRSGTLATPLCVGFGEACAIAKAEMGAENERLMGLRNRLLGGLRQRLPEIYVNGDLDHRIPGNLNIS
FAFVEGEGLMMGVKELAVSSGSACTSASLEPSYVLRALGVDVEMAHTSLRFGLGRFTTDEDIEFAIDHITEAVEHLRAMS
PLWDMHLEGVDLKSIEWAEH

Sequences:

>Translated_420_residues
MQMTALGKTTRPSLPRAPGAPVYLDYQATTPCDPRVVEVMLPWFTEKFGNPHSRNHRYGWEAEEAVEKAREQIADIIGAD
SKEVIFTSGATESNNLAIKGVAHFYKEKKNHIVTVVTEHKCVLDTCRYLEQEGFAVTYLPVKADGLIDLAELEAAITDQT
VIVSVMAVNNEIGVIQPLAEIGALCRKKGAFFHTDCAQAVGKIPLDVNAMNIDLMSISGHKLYGPKGIGALYVRRRPRVR
LVPLITGGGQERGMRSGTLATPLCVGFGEACAIAKAEMGAENERLMGLRNRLLGGLRQRLPEIYVNGDLDHRIPGNLNIS
FAFVEGEGLMMGVKELAVSSGSACTSASLEPSYVLRALGVDVEMAHTSLRFGLGRFTTDEDIEFAIDHITEAVEHLRAMS
PLWDMHLEGVDLKSIEWAEH
>Mature_420_residues
MQMTALGKTTRPSLPRAPGAPVYLDYQATTPCDPRVVEVMLPWFTEKFGNPHSRNHRYGWEAEEAVEKAREQIADIIGAD
SKEVIFTSGATESNNLAIKGVAHFYKEKKNHIVTVVTEHKCVLDTCRYLEQEGFAVTYLPVKADGLIDLAELEAAITDQT
VIVSVMAVNNEIGVIQPLAEIGALCRKKGAFFHTDCAQAVGKIPLDVNAMNIDLMSISGHKLYGPKGIGALYVRRRPRVR
LVPLITGGGQERGMRSGTLATPLCVGFGEACAIAKAEMGAENERLMGLRNRLLGGLRQRLPEIYVNGDLDHRIPGNLNIS
FAFVEGEGLMMGVKELAVSSGSACTSASLEPSYVLRALGVDVEMAHTSLRFGLGRFTTDEDIEFAIDHITEAVEHLRAMS
PLWDMHLEGVDLKSIEWAEH

Specific function: Catalyzes the removal of elemental sulfur from cysteine to produce alanine [H]

COG id: COG1104

COG function: function code E; Cysteine sulfinate desulfinase/cysteine desulfurase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-V pyridoxal-phosphate-dependent aminotransferase family. NifS/IscS subfamily [H]

Homologues:

Organism=Homo sapiens, GI32307132, Length=400, Percent_Identity=62.5, Blast_Score=561, Evalue=1e-160,
Organism=Homo sapiens, GI156713448, Length=417, Percent_Identity=34.2925659472422, Blast_Score=214, Evalue=2e-55,
Organism=Escherichia coli, GI48994898, Length=402, Percent_Identity=62.1890547263682, Blast_Score=535, Evalue=1e-153,
Organism=Escherichia coli, GI1789175, Length=240, Percent_Identity=31.25, Blast_Score=107, Evalue=2e-24,
Organism=Escherichia coli, GI1787970, Length=241, Percent_Identity=30.7053941908714, Blast_Score=105, Evalue=5e-24,
Organism=Caenorhabditis elegans, GI25143064, Length=406, Percent_Identity=62.0689655172414, Blast_Score=547, Evalue=1e-156,
Organism=Caenorhabditis elegans, GI17533177, Length=327, Percent_Identity=32.7217125382263, Blast_Score=166, Evalue=3e-41,
Organism=Saccharomyces cerevisiae, GI6319831, Length=400, Percent_Identity=60.25, Blast_Score=516, Evalue=1e-147,
Organism=Drosophila melanogaster, GI20129463, Length=402, Percent_Identity=64.6766169154229, Blast_Score=560, Evalue=1e-160,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000192
- InterPro:   IPR020578
- InterPro:   IPR010240
- InterPro:   IPR016454
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF00266 Aminotran_5 [H]

EC number: =2.8.1.7 [H]

Molecular weight: Translated: 45955; Mature: 45955

Theoretical pI: Translated: 5.92; Mature: 5.92

Prosite motif: PS00595 AA_TRANSFER_CLASS_5

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQMTALGKTTRPSLPRAPGAPVYLDYQATTPCDPRVVEVMLPWFTEKFGNPHSRNHRYGW
CCCCCCCCCCCCCCCCCCCCCEEEEECCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCC
EAEEAVEKAREQIADIIGADSKEVIFTSGATESNNLAIKGVAHFYKEKKNHIVTVVTEHK
CHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCEEEHHHHHHHHHCCCCEEEEEECCH
CVLDTCRYLEQEGFAVTYLPVKADGLIDLAELEAAITDQTVIVSVMAVNNEIGVIQPLAE
HHHHHHHHHHHCCCEEEEEEEECCCCEEHHHHHHHHCCCEEEEEEEEECCCCCHHHHHHH
IGALCRKKGAFFHTDCAQAVGKIPLDVNAMNIDLMSISGHKLYGPKGIGALYVRRRPRVR
HHHHHHHCCCEEECHHHHHHCCCCCEEEEEEEEEEEECCCEEECCCCCCEEEEECCCCEE
LVPLITGGGQERGMRSGTLATPLCVGFGEACAIAKAEMGAENERLMGLRNRLLGGLRQRL
EEEEEECCCHHCCCCCCCCCCHHHHCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHC
PEIYVNGDLDHRIPGNLNISFAFVEGEGLMMGVKELAVSSGSACTSASLEPSYVLRALGV
CEEEECCCCCCCCCCCCEEEEEEEECCCEEEEHHHHHHCCCCCCCCCCCCHHHHHHHHCC
DVEMAHTSLRFGLGRFTTDEDIEFAIDHITEAVEHLRAMSPLWDMHLEGVDLKSIEWAEH
CHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCHHHHCCCCCCCCCCCCCCC
>Mature Secondary Structure
MQMTALGKTTRPSLPRAPGAPVYLDYQATTPCDPRVVEVMLPWFTEKFGNPHSRNHRYGW
CCCCCCCCCCCCCCCCCCCCCEEEEECCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCC
EAEEAVEKAREQIADIIGADSKEVIFTSGATESNNLAIKGVAHFYKEKKNHIVTVVTEHK
CHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCEEEHHHHHHHHHCCCCEEEEEECCH
CVLDTCRYLEQEGFAVTYLPVKADGLIDLAELEAAITDQTVIVSVMAVNNEIGVIQPLAE
HHHHHHHHHHHCCCEEEEEEEECCCCEEHHHHHHHHCCCEEEEEEEEECCCCCHHHHHHH
IGALCRKKGAFFHTDCAQAVGKIPLDVNAMNIDLMSISGHKLYGPKGIGALYVRRRPRVR
HHHHHHHCCCEEECHHHHHHCCCCCEEEEEEEEEEEECCCEEECCCCCCEEEEECCCCEE
LVPLITGGGQERGMRSGTLATPLCVGFGEACAIAKAEMGAENERLMGLRNRLLGGLRQRL
EEEEEECCCHHCCCCCCCCCCHHHHCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHC
PEIYVNGDLDHRIPGNLNISFAFVEGEGLMMGVKELAVSSGSACTSASLEPSYVLRALGV
CEEEECCCCCCCCCCCCEEEEEEEECCCEEEEHHHHHHCCCCCCCCCCCCHHHHHHHHCC
DVEMAHTSLRFGLGRFTTDEDIEFAIDHITEAVEHLRAMSPLWDMHLEGVDLKSIEWAEH
CHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCHHHHCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA