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The map label for this gene is nqo13 [H]

Identifier: 83311874

GI number: 83311874

Start: 2960950

End: 2962464

Strand: Reverse

Name: nqo13 [H]

Synonym: amb2775

Alternate gene names: 83311874

Gene position: 2962464-2960950 (Counterclockwise)

Preceding gene: 83311875

Following gene: 83311873

Centisome position: 59.64

GC content: 62.44

Gene sequence:

>1515_bases
ATGAACGACTGGCCCCTTCTCTCGCTGACCACGTTCCTGCCCCTGGCCGGAGCGCTGTTCATCCTCACCATCCGCGGCGA
GACCGAGGTGGTGGCCCGCAACTCCCGTAACGTGGCGTTGCTGACCTCCATCGCGACCTTCGTGGTGTCCCTGTTCATCC
TGGCGAAGTTCAACGGCGCCTCGCCGGACTTCCAGCTCAAGGAGACGGCCCTTTGGTTCCCTGGAACCACCATCGCCTAT
TCCATGGGCGTGGACGGCATCTCCATGTGGTTCGTGCTGCTGTCGACCTTCCTGACCCCCATCTGCATCCTGGCCGCCTT
CGGCAGCGTGGAGAAGCGGGTCAAGGAATATATGGTCGCCTTCATGATCCTCGAGACCATGATGATCGGCATGTTCTGCG
CCCTTGATCTGGTGCTGTTCTACATCTTCTTCGAGGCCGTCCTGATCCCGATGTTCATCATCATCGGCGTGTGGGGCGGG
CAGCGCCGGGTCTACGCCGCGTTCAAGTTCTTCCTGTACACCCTGCTGGGATCGGTGCTGATGCTGATCGCCATGCTGGC
CATGTACTTCGATGCCCACACCACCGACATTCCGTCTCTGATGGCGCATTCGTTCCCCTTCAAGATGCAGCTGTGGCTGT
GGCTGGCCTTCTTCGCCTCCTTCGCGGTGAAGGTTCCCATGTGGCCGGTGCATACCTGGTTGCCCGACGCCCACGTGGAA
GCGCCCACCGGCGGTTCGGTGATCCTGGCCGGCGTGCTGCTGAAGATGGGGGCCTATGGCTTCATCCGCTTCTCCATTCC
CATGCTGCCGGATGCGTCGGTGTACTTCACGCCGCTGATCTACACCCTGTCCATCGTCGCGGTGATCTACACCTCGCTGG
TGGCGCTGGTGCAGGACGACATGAAGAAGCTGATCGCCTATTCCTCTGTGGCCCACATGGGTTACGTGACCATCGGCATC
TTCTCCATGACCACCCAGGGCGTGGAGGGCGCGGTGTTCCAGATGCTGTCCCACGGCATCGTGGCCAGCGCCCTGTTCCT
CTGCGTCGGCGTGATCTACGACCGCATGCATACCCGCGAGATCGCCCGTTACGGCGGTCTGGCCACCCGCATGCCCATCT
ATGCCGTGGTGTTCATGCTGTTCACCATGGCTTCGGTGGGCCTGCCGGGTACCGCCGGGTTCGTGGGCGAGTTCCTGGTG
CTGATGGGCGTGTTCCAGGTCAACACCTGGGTGGCGTTCTTCGCCGCCACTGGTCTGGTTCTGGGCTGCACCTACATGCT
GTACCTCTATCGCCGGGTGGTGTTCGGCCGCCTGACCCGCGAGGACCTGAAGGGCATCCTGGACATGACCCCGCGTGAAA
TCGCGGTGTTCGCTCCGCTGATCGTCCTGACCATCTGGATGGGTGTCGCTCCCGGCACCTTCCTCGAGCCGATGCATGCG
TCGGTGACCAAGCTGATCTCCAATTACGAGCTTGCCCGGGCCGCCCATGACGGCCTGTCGCTGGCTGCCCGCTGA

Upstream 100 bases:

>100_bases
CGGCTACCTCTACCACTACGCCTACGCCATGCTGATCGGGGTGGCGGCCTTCGTCACCTGGTACATTTTCAACGCGCGCT
GAGGACGAGGAAGACGCCCC

Downstream 100 bases:

>100_bases
GGGGGACCTAAGACGTGATCAAGACTCTCGACCTCGTCCCGGTCCTGCCGGAAATCTTCATGGCAGTGGCCGGTCTGGCC
CTGCTGATGCTGGGCGTGTT

Product: NADH dehydrogenase subunit M

Products: NA

Alternate protein names: NADH dehydrogenase I, chain 13; NDH-1, chain 13 [H]

Number of amino acids: Translated: 504; Mature: 504

Protein sequence:

>504_residues
MNDWPLLSLTTFLPLAGALFILTIRGETEVVARNSRNVALLTSIATFVVSLFILAKFNGASPDFQLKETALWFPGTTIAY
SMGVDGISMWFVLLSTFLTPICILAAFGSVEKRVKEYMVAFMILETMMIGMFCALDLVLFYIFFEAVLIPMFIIIGVWGG
QRRVYAAFKFFLYTLLGSVLMLIAMLAMYFDAHTTDIPSLMAHSFPFKMQLWLWLAFFASFAVKVPMWPVHTWLPDAHVE
APTGGSVILAGVLLKMGAYGFIRFSIPMLPDASVYFTPLIYTLSIVAVIYTSLVALVQDDMKKLIAYSSVAHMGYVTIGI
FSMTTQGVEGAVFQMLSHGIVASALFLCVGVIYDRMHTREIARYGGLATRMPIYAVVFMLFTMASVGLPGTAGFVGEFLV
LMGVFQVNTWVAFFAATGLVLGCTYMLYLYRRVVFGRLTREDLKGILDMTPREIAVFAPLIVLTIWMGVAPGTFLEPMHA
SVTKLISNYELARAAHDGLSLAAR

Sequences:

>Translated_504_residues
MNDWPLLSLTTFLPLAGALFILTIRGETEVVARNSRNVALLTSIATFVVSLFILAKFNGASPDFQLKETALWFPGTTIAY
SMGVDGISMWFVLLSTFLTPICILAAFGSVEKRVKEYMVAFMILETMMIGMFCALDLVLFYIFFEAVLIPMFIIIGVWGG
QRRVYAAFKFFLYTLLGSVLMLIAMLAMYFDAHTTDIPSLMAHSFPFKMQLWLWLAFFASFAVKVPMWPVHTWLPDAHVE
APTGGSVILAGVLLKMGAYGFIRFSIPMLPDASVYFTPLIYTLSIVAVIYTSLVALVQDDMKKLIAYSSVAHMGYVTIGI
FSMTTQGVEGAVFQMLSHGIVASALFLCVGVIYDRMHTREIARYGGLATRMPIYAVVFMLFTMASVGLPGTAGFVGEFLV
LMGVFQVNTWVAFFAATGLVLGCTYMLYLYRRVVFGRLTREDLKGILDMTPREIAVFAPLIVLTIWMGVAPGTFLEPMHA
SVTKLISNYELARAAHDGLSLAAR
>Mature_504_residues
MNDWPLLSLTTFLPLAGALFILTIRGETEVVARNSRNVALLTSIATFVVSLFILAKFNGASPDFQLKETALWFPGTTIAY
SMGVDGISMWFVLLSTFLTPICILAAFGSVEKRVKEYMVAFMILETMMIGMFCALDLVLFYIFFEAVLIPMFIIIGVWGG
QRRVYAAFKFFLYTLLGSVLMLIAMLAMYFDAHTTDIPSLMAHSFPFKMQLWLWLAFFASFAVKVPMWPVHTWLPDAHVE
APTGGSVILAGVLLKMGAYGFIRFSIPMLPDASVYFTPLIYTLSIVAVIYTSLVALVQDDMKKLIAYSSVAHMGYVTIGI
FSMTTQGVEGAVFQMLSHGIVASALFLCVGVIYDRMHTREIARYGGLATRMPIYAVVFMLFTMASVGLPGTAGFVGEFLV
LMGVFQVNTWVAFFAATGLVLGCTYMLYLYRRVVFGRLTREDLKGILDMTPREIAVFAPLIVLTIWMGVAPGTFLEPMHA
SVTKLISNYELARAAHDGLSLAAR

Specific function: NDH-1 shuttles electrons from NADH, via FMN and iron- sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be ubiquinone. Couples the redox reaction to proton translocat

COG id: COG1008

COG function: function code C; NADH:ubiquinone oxidoreductase subunit 4 (chain M)

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I subunit 4 family [H]

Homologues:

Organism=Homo sapiens, GI251831116, Length=368, Percent_Identity=34.7826086956522, Blast_Score=194, Evalue=1e-49,
Organism=Homo sapiens, GI251831117, Length=391, Percent_Identity=25.5754475703325, Blast_Score=87, Evalue=4e-17,
Organism=Escherichia coli, GI1788613, Length=439, Percent_Identity=37.129840546697, Blast_Score=290, Evalue=1e-79,
Organism=Escherichia coli, GI1788827, Length=282, Percent_Identity=26.9503546099291, Blast_Score=124, Evalue=1e-29,
Organism=Escherichia coli, GI1788831, Length=278, Percent_Identity=28.4172661870504, Blast_Score=99, Evalue=5e-22,
Organism=Escherichia coli, GI1788614, Length=481, Percent_Identity=25.7796257796258, Blast_Score=93, Evalue=5e-20,
Organism=Escherichia coli, GI2367154, Length=257, Percent_Identity=24.5136186770428, Blast_Score=80, Evalue=3e-16,
Organism=Escherichia coli, GI145693160, Length=383, Percent_Identity=25.065274151436, Blast_Score=80, Evalue=4e-16,
Organism=Escherichia coli, GI1788829, Length=342, Percent_Identity=24.5614035087719, Blast_Score=74, Evalue=2e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010227
- InterPro:   IPR001750
- InterPro:   IPR003918
- InterPro:   IPR000260 [H]

Pfam domain/function: PF00361 Oxidored_q1; PF01059 Oxidored_q5_N [H]

EC number: =1.6.99.5 [H]

Molecular weight: Translated: 55775; Mature: 55775

Theoretical pI: Translated: 7.98; Mature: 7.98

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
6.0 %Met     (Translated Protein)
6.7 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
6.0 %Met     (Mature Protein)
6.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNDWPLLSLTTFLPLAGALFILTIRGETEVVARNSRNVALLTSIATFVVSLFILAKFNGA
CCCCCHHHHHHHHHHHHHHEEEEECCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHCCCC
SPDFQLKETALWFPGTTIAYSMGVDGISMWFVLLSTFLTPICILAAFGSVEKRVKEYMVA
CCCCEECCCEEECCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FMILETMMIGMFCALDLVLFYIFFEAVLIPMFIIIGVWGGQRRVYAAFKFFLYTLLGSVL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHH
MLIAMLAMYFDAHTTDIPSLMAHSFPFKMQLWLWLAFFASFAVKVPMWPVHTWLPDAHVE
HHHHHHHHHHCCCCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCHHHCCCCCCCC
APTGGSVILAGVLLKMGAYGFIRFSIPMLPDASVYFTPLIYTLSIVAVIYTSLVALVQDD
CCCCCHHHHHHHHHHHCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
MKKLIAYSSVAHMGYVTIGIFSMTTQGVEGAVFQMLSHGIVASALFLCVGVIYDRMHTRE
HHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IARYGGLATRMPIYAVVFMLFTMASVGLPGTAGFVGEFLVLMGVFQVNTWVAFFAATGLV
HHHHCCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LGCTYMLYLYRRVVFGRLTREDLKGILDMTPREIAVFAPLIVLTIWMGVAPGTFLEPMHA
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHH
SVTKLISNYELARAAHDGLSLAAR
HHHHHHHHHHHHHHHHCCCHHCCC
>Mature Secondary Structure
MNDWPLLSLTTFLPLAGALFILTIRGETEVVARNSRNVALLTSIATFVVSLFILAKFNGA
CCCCCHHHHHHHHHHHHHHEEEEECCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHCCCC
SPDFQLKETALWFPGTTIAYSMGVDGISMWFVLLSTFLTPICILAAFGSVEKRVKEYMVA
CCCCEECCCEEECCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FMILETMMIGMFCALDLVLFYIFFEAVLIPMFIIIGVWGGQRRVYAAFKFFLYTLLGSVL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHH
MLIAMLAMYFDAHTTDIPSLMAHSFPFKMQLWLWLAFFASFAVKVPMWPVHTWLPDAHVE
HHHHHHHHHHCCCCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCHHHCCCCCCCC
APTGGSVILAGVLLKMGAYGFIRFSIPMLPDASVYFTPLIYTLSIVAVIYTSLVALVQDD
CCCCCHHHHHHHHHHHCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
MKKLIAYSSVAHMGYVTIGIFSMTTQGVEGAVFQMLSHGIVASALFLCVGVIYDRMHTRE
HHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IARYGGLATRMPIYAVVFMLFTMASVGLPGTAGFVGEFLVLMGVFQVNTWVAFFAATGLV
HHHHCCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LGCTYMLYLYRRVVFGRLTREDLKGILDMTPREIAVFAPLIVLTIWMGVAPGTFLEPMHA
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHH
SVTKLISNYELARAAHDGLSLAAR
HHHHHHHHHHHHHHHHCCCHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8422400 [H]