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The map label for this gene is nuoN

Identifier: 83311873

GI number: 83311873

Start: 2959472

End: 2960935

Strand: Reverse

Name: nuoN

Synonym: amb2774

Alternate gene names: 83311873

Gene position: 2960935-2959472 (Counterclockwise)

Preceding gene: 83311874

Following gene: 83311872

Centisome position: 59.61

GC content: 64.55

Gene sequence:

>1464_bases
GTGATCAAGACTCTCGACCTCGTCCCGGTCCTGCCGGAAATCTTCATGGCAGTGGCCGGTCTGGCCCTGCTGATGCTGGG
CGTGTTCCGCAAGGAGGACAGCACCAAGTCGGTGTCGGTCCTGGTGATCCTGGCGCTTGGCGCCGCCATGGTGCTGGTGT
CGTCCCTGGGGGGCGAGCGGCTGACGGCCTTCAACGGCCTGTTCGTCGCCGACCGCTTCGCCGGCTTCGCCAAGGGGCTG
GTGCTGGTGGCCTCGGCCATCGCCACCGCCATGTCGCTGCCCTATCTGGAACGCGAGAAGATCGGCCGCTTCGAGTATCC
GGTGCTGGTGCTGTTCGCCACCCTGGGCATGATGATGATGATTTCGGCCAACGATTTCATCGCACTGTACCTGGGCCTCG
AACTGCAGAGCCTGGCGCTCTATGTGCTGGCCGCCTATAACCGCGACAACGCCCGCGCCACCGAGGCGGGCCTCAAGTAC
TTCGTGCTGGGCTCGCTGGCCTCGGGCCTGCTGCTGTACGGCATCTCGCTGCTCTACGGCTTTGCCGGCACCACCTCGTT
CGAGGGGCTGGCCAATCTGTTCGCCGGCGGCCACGACCATCCGATCAAGCCCAATATGGGCATCATCGCCGGTTTGGTCT
TCGTGCTGGCCGGTCTGTCGTTCAAGGTCTCGGCGGTGCCGTTCCACATGTGGGCGCCCGACGTCTACGAGGGCGCACCC
ACCCCGGTGACCTCCTTCTTCGCCGTGGCCCCCAAGATCGCCGCCCTGTGCCTGCTGGTGCGCGTCATGACCGGTCCCTT
CGCCGATCTGGTGGAGCAGTGGCGTCAGGTGGTGACCTTCATCGCCATCGGCTCCATGTTCGTCGGCTCCTTCGCCGCCG
TGGTCCAGACCAACATCAAGCGTCTGATGGCCTATTCCTCCATCGGCCATGTGGGCTTCGTGCTGGTCGGCATCGCCGCC
GGGTCGACGCTCGGCATCCAGGGCGTGCTGATCTATCTCGCCATCTACCTGTTCATGAATGTGGGCGCCTTCGCGGTGAT
CCTGTCCATGCGCCAGAAGGGCCGGATGGTCGAGGGCATCGATGACCTGGCCGGTCTGTCCAAGACCCACCCCATGATGG
CCTTCGTCATGGCGGTGCTGATGTTCTCCATGGCGGGCGTGCCGCCGCTGGCCGGGTTCTGGGGCAAGTTCTACGTGTTC
ATGGCCGCCATCGAGTCGGGTCTTTACACCCTGTCCATCCTGGGCGTCCTGTCCTCGGTGGTGAGCACCTATTACTACCT
GCGCATCGTCAAGGTCATGTACTTCGACGAGCCGGTCGAGGCTTTCGACAAGCCGGTGGGCACCTCCATGACCCTGGTGA
TGGCGGTTTCCACCATCGTCATCCTAGCCTTTACCCTGATCCCGGCCCCGCTGGTGACCAGCGCCAAGGCCGCCGCCCAG
GTTCTGTTCCCGGCGGCGGGATGA

Upstream 100 bases:

>100_bases
CGATGCATGCGTCGGTGACCAAGCTGATCTCCAATTACGAGCTTGCCCGGGCCGCCCATGACGGCCTGTCGCTGGCTGCC
CGCTGAGGGGGACCTAAGAC

Downstream 100 bases:

>100_bases
CAACCTTCGATCTGCCGGCGCCGTTCAGCCATGTGGAGCTGGACAGCGTCGGCAGCACCAATGACGAGGTTCGGCGGCGG
GTCGAGGACGGCTCCGCCGC

Product: NADH dehydrogenase subunit N

Products: NA

Alternate protein names: NADH dehydrogenase I subunit N; NDH-1 subunit N

Number of amino acids: Translated: 487; Mature: 487

Protein sequence:

>487_residues
MIKTLDLVPVLPEIFMAVAGLALLMLGVFRKEDSTKSVSVLVILALGAAMVLVSSLGGERLTAFNGLFVADRFAGFAKGL
VLVASAIATAMSLPYLEREKIGRFEYPVLVLFATLGMMMMISANDFIALYLGLELQSLALYVLAAYNRDNARATEAGLKY
FVLGSLASGLLLYGISLLYGFAGTTSFEGLANLFAGGHDHPIKPNMGIIAGLVFVLAGLSFKVSAVPFHMWAPDVYEGAP
TPVTSFFAVAPKIAALCLLVRVMTGPFADLVEQWRQVVTFIAIGSMFVGSFAAVVQTNIKRLMAYSSIGHVGFVLVGIAA
GSTLGIQGVLIYLAIYLFMNVGAFAVILSMRQKGRMVEGIDDLAGLSKTHPMMAFVMAVLMFSMAGVPPLAGFWGKFYVF
MAAIESGLYTLSILGVLSSVVSTYYYLRIVKVMYFDEPVEAFDKPVGTSMTLVMAVSTIVILAFTLIPAPLVTSAKAAAQ
VLFPAAG

Sequences:

>Translated_487_residues
MIKTLDLVPVLPEIFMAVAGLALLMLGVFRKEDSTKSVSVLVILALGAAMVLVSSLGGERLTAFNGLFVADRFAGFAKGL
VLVASAIATAMSLPYLEREKIGRFEYPVLVLFATLGMMMMISANDFIALYLGLELQSLALYVLAAYNRDNARATEAGLKY
FVLGSLASGLLLYGISLLYGFAGTTSFEGLANLFAGGHDHPIKPNMGIIAGLVFVLAGLSFKVSAVPFHMWAPDVYEGAP
TPVTSFFAVAPKIAALCLLVRVMTGPFADLVEQWRQVVTFIAIGSMFVGSFAAVVQTNIKRLMAYSSIGHVGFVLVGIAA
GSTLGIQGVLIYLAIYLFMNVGAFAVILSMRQKGRMVEGIDDLAGLSKTHPMMAFVMAVLMFSMAGVPPLAGFWGKFYVF
MAAIESGLYTLSILGVLSSVVSTYYYLRIVKVMYFDEPVEAFDKPVGTSMTLVMAVSTIVILAFTLIPAPLVTSAKAAAQ
VLFPAAG
>Mature_487_residues
MIKTLDLVPVLPEIFMAVAGLALLMLGVFRKEDSTKSVSVLVILALGAAMVLVSSLGGERLTAFNGLFVADRFAGFAKGL
VLVASAIATAMSLPYLEREKIGRFEYPVLVLFATLGMMMMISANDFIALYLGLELQSLALYVLAAYNRDNARATEAGLKY
FVLGSLASGLLLYGISLLYGFAGTTSFEGLANLFAGGHDHPIKPNMGIIAGLVFVLAGLSFKVSAVPFHMWAPDVYEGAP
TPVTSFFAVAPKIAALCLLVRVMTGPFADLVEQWRQVVTFIAIGSMFVGSFAAVVQTNIKRLMAYSSIGHVGFVLVGIAA
GSTLGIQGVLIYLAIYLFMNVGAFAVILSMRQKGRMVEGIDDLAGLSKTHPMMAFVMAVLMFSMAGVPPLAGFWGKFYVF
MAAIESGLYTLSILGVLSSVVSTYYYLRIVKVMYFDEPVEAFDKPVGTSMTLVMAVSTIVILAFTLIPAPLVTSAKAAAQ
VLFPAAG

Specific function: NDH-1 shuttles electrons from NADH, via FMN and iron- sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be ubiquinone. Couples the redox reaction to proton translocat

COG id: COG1007

COG function: function code C; NADH:ubiquinone oxidoreductase subunit 2 (chain N)

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I subunit 2 family

Homologues:

Organism=Homo sapiens, GI251831108, Length=240, Percent_Identity=22.9166666666667, Blast_Score=74, Evalue=4e-13,
Organism=Escherichia coli, GI145693160, Length=487, Percent_Identity=35.1129363449692, Blast_Score=253, Evalue=3e-68,
Organism=Escherichia coli, GI1788614, Length=351, Percent_Identity=25.3561253561254, Blast_Score=87, Evalue=3e-18,
Organism=Escherichia coli, GI1788831, Length=483, Percent_Identity=22.7743271221532, Blast_Score=84, Evalue=3e-17,
Organism=Escherichia coli, GI1788613, Length=219, Percent_Identity=28.310502283105, Blast_Score=74, Evalue=2e-14,
Organism=Escherichia coli, GI2367154, Length=334, Percent_Identity=26.9461077844311, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI1788827, Length=312, Percent_Identity=25, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI1788829, Length=387, Percent_Identity=23.7726098191214, Blast_Score=63, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NUON_MAGSA (Q2W3J7)

Other databases:

- EMBL:   AP007255
- RefSeq:   YP_422137.1
- STRING:   Q2W3J7
- GeneID:   3803864
- GenomeReviews:   AP007255_GR
- KEGG:   mag:amb2774
- NMPDR:   fig|342108.5.peg.2461
- HOGENOM:   HBG747830
- OMA:   EYPVLVL
- PhylomeDB:   Q2W3J7
- ProtClustDB:   PRK05777
- BioCyc:   MMAG342108:AMB2774-MONOMER
- GO:   GO:0006810
- HAMAP:   MF_00445
- InterPro:   IPR010096
- InterPro:   IPR001750
- TIGRFAMs:   TIGR01770

Pfam domain/function: PF00361 Oxidored_q1

EC number: =1.6.99.5

Molecular weight: Translated: 51859; Mature: 51859

Theoretical pI: Translated: 8.71; Mature: 8.71

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

HASH(0x138834c8)-; HASH(0x1375369c)-; HASH(0x13378b80)-; HASH(0x13c4b4c4)-; HASH(0x13b5feb8)-; HASH(0x1341f00c)-; HASH(0x13819bec)-; HASH(0xb731154)-; HASH(0x139ba880)-; HASH(0x133d4b10)-; HASH(0x13e68dd4)-; HASH(0x13948c18)-; HASH(0x1320550c)-;

Cys/Met content:

0.2 %Cys     (Translated Protein)
5.3 %Met     (Translated Protein)
5.5 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
5.3 %Met     (Mature Protein)
5.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIKTLDLVPVLPEIFMAVAGLALLMLGVFRKEDSTKSVSVLVILALGAAMVLVSSLGGER
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCCH
LTAFNGLFVADRFAGFAKGLVLVASAIATAMSLPYLEREKIGRFEYPVLVLFATLGMMMM
HHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHCCCCCCHHHHHHHHHHHHHH
ISANDFIALYLGLELQSLALYVLAAYNRDNARATEAGLKYFVLGSLASGLLLYGISLLYG
HCCCCCEEEEECHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FAGTTSFEGLANLFAGGHDHPIKPNMGIIAGLVFVLAGLSFKVSAVPFHMWAPDVYEGAP
HCCCCHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHCCCEEEEECEEECCCCHHCCCCC
TPVTSFFAVAPKIAALCLLVRVMTGPFADLVEQWRQVVTFIAIGSMFVGSFAAVVQTNIK
CCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RLMAYSSIGHVGFVLVGIAAGSTLGIQGVLIYLAIYLFMNVGAFAVILSMRQKGRMVEGI
HHHHHHHCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHCH
DDLAGLSKTHPMMAFVMAVLMFSMAGVPPLAGFWGKFYVFMAAIESGLYTLSILGVLSSV
HHHHCCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHH
VSTYYYLRIVKVMYFDEPVEAFDKPVGTSMTLVMAVSTIVILAFTLIPAPLVTSAKAAAQ
HHHHHHHHHHHHHHCCCHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHH
VLFPAAG
HHCCCCH
>Mature Secondary Structure
MIKTLDLVPVLPEIFMAVAGLALLMLGVFRKEDSTKSVSVLVILALGAAMVLVSSLGGER
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCCH
LTAFNGLFVADRFAGFAKGLVLVASAIATAMSLPYLEREKIGRFEYPVLVLFATLGMMMM
HHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHCCCCCCHHHHHHHHHHHHHH
ISANDFIALYLGLELQSLALYVLAAYNRDNARATEAGLKYFVLGSLASGLLLYGISLLYG
HCCCCCEEEEECHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FAGTTSFEGLANLFAGGHDHPIKPNMGIIAGLVFVLAGLSFKVSAVPFHMWAPDVYEGAP
HCCCCHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHCCCEEEEECEEECCCCHHCCCCC
TPVTSFFAVAPKIAALCLLVRVMTGPFADLVEQWRQVVTFIAIGSMFVGSFAAVVQTNIK
CCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RLMAYSSIGHVGFVLVGIAAGSTLGIQGVLIYLAIYLFMNVGAFAVILSMRQKGRMVEGI
HHHHHHHCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHCH
DDLAGLSKTHPMMAFVMAVLMFSMAGVPPLAGFWGKFYVFMAAIESGLYTLSILGVLSSV
HHHHCCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHH
VSTYYYLRIVKVMYFDEPVEAFDKPVGTSMTLVMAVSTIVILAFTLIPAPLVTSAKAAAQ
HHHHHHHHHHHHHHCCCHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHH
VLFPAAG
HHCCCCH

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA