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The map label for this gene is dosC [H]

Identifier: 83311629

GI number: 83311629

Start: 2713248

End: 2714408

Strand: Reverse

Name: dosC [H]

Synonym: amb2530

Alternate gene names: 83311629

Gene position: 2714408-2713248 (Counterclockwise)

Preceding gene: 83311633

Following gene: 83311628

Centisome position: 54.65

GC content: 66.75

Gene sequence:

>1161_bases
TTGATCCTTGATCTGCATACCATGCTGGTGGCGGTCGCCGTGGCGACGGCGTGCTGCGCCATTGCCCGAATCGTCTTGTA
CATCCTGCATCCGGGCCTGGCCGGGCTGGGGTATTGGGCCTGGGCCAGCGTGATCGGGGCGGCAAGCTTCGCCGTGGCGG
GAACCGGGGCGGGAATGCCCGAGGGCTGGTCCCTGTCCCTGGCCCACGGGCTGGTGGTGGCGGGGTTCTGCCTGGTCTGG
GACGGGTTTCGCCGCTTTCTCGGGCGCCCGGGGCTGACGTCCCGGTTCTATCTCGGGCTCGGCGGCGCGGCCGTGGTGAT
GATCGTCGGGAGTCATCTGGCCGGATCGCTGCACCTGCGCGCCACCTTCAACTCAGCCCTGGTGATGGCGATTTCCGCGG
CCATCGCCCGGGAATTGCTGTGGCGCACGCCGCCCCATCGTCTGGCCATGCGTCTGGCCGGGTGGGTCTATTTCGCCAAT
GCGCTGTTCTTCCTGGTGCGCGGGCTCTCCATCGGCTTCGATCTCGAATTCATGGCCGGCAAGATGTCCTACGGCATGAC
GGTGGTCGCCAGCATGTGGTGGCTGTGCGTCACCATCTCGGTGACGCTGTGCATGGTGCTGATGGCCAGCGAGCGGCTGC
AGGCGGATTTGAACGAACAGGCCAGCCGCGATCCGCTGACCGGGGCGCTGAACCGGCGGGCCTTCGCCTCCCTGGGGGAG
AGAGAGATCGTGCGGGCCCGGCGCACGGCCAGCCCGTTGTCGCTGCTGATGATCGACATGGATCATTTCAAGCAGATCAA
CGACTGCCTGGGCCATGGGGGCGGTGACGAGGTGCTGGTGCTGTTCGCCACCCTGGCGGGACGTATCCTGAGGGCCGAGG
ATTTGTTCTGTCGCTTTGGCGGCGAGGAGTTTCTGGCACTGCTGCCCAGCAGCTCCCTTATGGAGGCCATGGGGGTGGCG
GAGCGCCTGCGCGCCGCTTTTTCCGCCGAAGGCGCCGGGCTGGATTCGGAGGGAAAATTGCCGTTTCCCATGACGCTCAG
TGTCGGCATCGCCGCTCTGGCGGCCGACGAGGACATGGAGGCGGCCATCCGCCGCGCCGATGCCGCCTTGTACCGGGCCA
AGGATTTGGGGCGTAACCGTTGTGAACTGGCCGGGGGATAG

Upstream 100 bases:

>100_bases
CCAATGCCGAATTCAAGTCATGGTTCGTCGGTTGCATATATGAGCCTGGGCTTATAGTCTCTCACAGTTGAAGGGTTCCA
ACCTGGGGAAGGACCCCGTC

Downstream 100 bases:

>100_bases
TTGCGTTCACCGTGCTTGGGCGCTAGTGTCCCGGACATGACCGAGTCCCGCTTCCATACCCTGGCCCCCTATGCCTGCCG
GCCCGAGGAATCGCGCGGCC

Product: GGDEF domain-containing protein

Products: NA

Alternate protein names: DGC [H]

Number of amino acids: Translated: 386; Mature: 386

Protein sequence:

>386_residues
MILDLHTMLVAVAVATACCAIARIVLYILHPGLAGLGYWAWASVIGAASFAVAGTGAGMPEGWSLSLAHGLVVAGFCLVW
DGFRRFLGRPGLTSRFYLGLGGAAVVMIVGSHLAGSLHLRATFNSALVMAISAAIARELLWRTPPHRLAMRLAGWVYFAN
ALFFLVRGLSIGFDLEFMAGKMSYGMTVVASMWWLCVTISVTLCMVLMASERLQADLNEQASRDPLTGALNRRAFASLGE
REIVRARRTASPLSLLMIDMDHFKQINDCLGHGGGDEVLVLFATLAGRILRAEDLFCRFGGEEFLALLPSSSLMEAMGVA
ERLRAAFSAEGAGLDSEGKLPFPMTLSVGIAALAADEDMEAAIRRADAALYRAKDLGRNRCELAGG

Sequences:

>Translated_386_residues
MILDLHTMLVAVAVATACCAIARIVLYILHPGLAGLGYWAWASVIGAASFAVAGTGAGMPEGWSLSLAHGLVVAGFCLVW
DGFRRFLGRPGLTSRFYLGLGGAAVVMIVGSHLAGSLHLRATFNSALVMAISAAIARELLWRTPPHRLAMRLAGWVYFAN
ALFFLVRGLSIGFDLEFMAGKMSYGMTVVASMWWLCVTISVTLCMVLMASERLQADLNEQASRDPLTGALNRRAFASLGE
REIVRARRTASPLSLLMIDMDHFKQINDCLGHGGGDEVLVLFATLAGRILRAEDLFCRFGGEEFLALLPSSSLMEAMGVA
ERLRAAFSAEGAGLDSEGKLPFPMTLSVGIAALAADEDMEAAIRRADAALYRAKDLGRNRCELAGG
>Mature_386_residues
MILDLHTMLVAVAVATACCAIARIVLYILHPGLAGLGYWAWASVIGAASFAVAGTGAGMPEGWSLSLAHGLVVAGFCLVW
DGFRRFLGRPGLTSRFYLGLGGAAVVMIVGSHLAGSLHLRATFNSALVMAISAAIARELLWRTPPHRLAMRLAGWVYFAN
ALFFLVRGLSIGFDLEFMAGKMSYGMTVVASMWWLCVTISVTLCMVLMASERLQADLNEQASRDPLTGALNRRAFASLGE
REIVRARRTASPLSLLMIDMDHFKQINDCLGHGGGDEVLVLFATLAGRILRAEDLFCRFGGEEFLALLPSSSLMEAMGVA
ERLRAAFSAEGAGLDSEGKLPFPMTLSVGIAALAADEDMEAAIRRADAALYRAKDLGRNRCELAGG

Specific function: Overexpression leads to an increased level of c-di-GMP, which leads to changes in the cell surface, to abnormal cell division, increased biofilm formation and decreased swimming (the latter 2 in strain W3110). In a strain able to produce cellulose (strain

COG id: COG2199

COG function: function code T; FOG: GGDEF domain

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 GGDEF domain [H]

Homologues:

Organism=Escherichia coli, GI145693134, Length=173, Percent_Identity=41.0404624277457, Blast_Score=122, Evalue=5e-29,
Organism=Escherichia coli, GI87081881, Length=180, Percent_Identity=41.1111111111111, Blast_Score=119, Evalue=5e-28,
Organism=Escherichia coli, GI1787262, Length=202, Percent_Identity=33.6633663366337, Blast_Score=110, Evalue=1e-25,
Organism=Escherichia coli, GI87082007, Length=238, Percent_Identity=33.6134453781513, Blast_Score=103, Evalue=2e-23,
Organism=Escherichia coli, GI1786584, Length=204, Percent_Identity=33.8235294117647, Blast_Score=101, Evalue=1e-22,
Organism=Escherichia coli, GI1787802, Length=194, Percent_Identity=30.9278350515464, Blast_Score=83, Evalue=3e-17,
Organism=Escherichia coli, GI1787816, Length=164, Percent_Identity=32.9268292682927, Blast_Score=82, Evalue=7e-17,
Organism=Escherichia coli, GI87081974, Length=190, Percent_Identity=27.8947368421053, Blast_Score=78, Evalue=1e-15,
Organism=Escherichia coli, GI1788381, Length=184, Percent_Identity=32.6086956521739, Blast_Score=76, Evalue=4e-15,
Organism=Escherichia coli, GI1788085, Length=161, Percent_Identity=30.4347826086957, Blast_Score=75, Evalue=5e-15,
Organism=Escherichia coli, GI1787541, Length=177, Percent_Identity=33.3333333333333, Blast_Score=70, Evalue=3e-13,
Organism=Escherichia coli, GI1788956, Length=167, Percent_Identity=33.5329341317365, Blast_Score=62, Evalue=8e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001054
- InterPro:   IPR000160
- InterPro:   IPR012292
- InterPro:   IPR009050 [H]

Pfam domain/function: PF00990 GGDEF [H]

EC number: =2.7.7.65 [H]

Molecular weight: Translated: 41277; Mature: 41277

Theoretical pI: Translated: 7.48; Mature: 7.48

Prosite motif: PS50887 GGDEF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
4.7 %Met     (Translated Protein)
6.7 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
4.7 %Met     (Mature Protein)
6.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MILDLHTMLVAVAVATACCAIARIVLYILHPGLAGLGYWAWASVIGAASFAVAGTGAGMP
CEEHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHEECCCCCCC
EGWSLSLAHGLVVAGFCLVWDGFRRFLGRPGLTSRFYLGLGGAAVVMIVGSHLAGSLHLR
CCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCHHHHHHHHHHHCCCCEEEE
ATFNSALVMAISAAIARELLWRTPPHRLAMRLAGWVYFANALFFLVRGLSIGFDLEFMAG
EHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHH
KMSYGMTVVASMWWLCVTISVTLCMVLMASERLQADLNEQASRDPLTGALNRRAFASLGE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCH
REIVRARRTASPLSLLMIDMDHFKQINDCLGHGGGDEVLVLFATLAGRILRAEDLFCRFG
HHHHHHHHCCCCHHHEEECHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHCC
GEEFLALLPSSSLMEAMGVAERLRAAFSAEGAGLDSEGKLPFPMTLSVGIAALAADEDME
CHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEHHHHHHHHHCCCHHH
AAIRRADAALYRAKDLGRNRCELAGG
HHHHHHHHHHHHHHHCCCCCCCCCCC
>Mature Secondary Structure
MILDLHTMLVAVAVATACCAIARIVLYILHPGLAGLGYWAWASVIGAASFAVAGTGAGMP
CEEHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHEECCCCCCC
EGWSLSLAHGLVVAGFCLVWDGFRRFLGRPGLTSRFYLGLGGAAVVMIVGSHLAGSLHLR
CCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCHHHHHHHHHHHCCCCEEEE
ATFNSALVMAISAAIARELLWRTPPHRLAMRLAGWVYFANALFFLVRGLSIGFDLEFMAG
EHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHH
KMSYGMTVVASMWWLCVTISVTLCMVLMASERLQADLNEQASRDPLTGALNRRAFASLGE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCH
REIVRARRTASPLSLLMIDMDHFKQINDCLGHGGGDEVLVLFATLAGRILRAEDLFCRFG
HHHHHHHHCCCCHHHEEECHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHCC
GEEFLALLPSSSLMEAMGVAERLRAAFSAEGAGLDSEGKLPFPMTLSVGIAALAADEDME
CHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEHHHHHHHHHCCCHHH
AAIRRADAALYRAKDLGRNRCELAGG
HHHHHHHHHHHHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503 [H]