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The map label for this gene is rpfG [H]

Identifier: 83310938

GI number: 83310938

Start: 1976331

End: 1977440

Strand: Reverse

Name: rpfG [H]

Synonym: amb1839

Alternate gene names: 83310938

Gene position: 1977440-1976331 (Counterclockwise)

Preceding gene: 83310939

Following gene: 83310936

Centisome position: 39.81

GC content: 63.15

Gene sequence:

>1110_bases
ATGCTGCCTGCGGATTTCATGGACAGGGCCACCATTCTGGTGGTGGACGACAGCCCCGATGACGTCTGGCTGATGAGCGA
ATTGCTCAAGGCCGATTACCGGGTCAAGGTGGCCAACAGCGGCGAAAGAGGCCTGAAGATCGCCATGGGCAACGAGCGGC
CCGACCTGGTCCTGCTCGACGTGATGATGCCCGACATCGACGGCTACGAAGTCTGCCGGCAACTCAAGACCGATTCCCGG
ACCCACGACATTCCAGTAATCTTCCTGACCAGCAAGACGCGCATGGCCGAGGAGAAGCGTGGCCTGGACATGGGCGCGGT
GGACTACATCACCAAGCCGTTCAGCCCCTCCATCGTGCTGGCCCGGGTCAAGGCGCACCTGCGGCTCAAGTCCTCGGCCG
ACTTCTTGCGCGACCAGAATCGCTTTTTGGAGCGCGAGGTCGCCAAGCGGACCAAGGACGTTACCGCCGTCAAGGACGTC
ACCATCCTGGCCATGGCCTCCCTGGCCGAGACCCGCGACCTGATGACCGGCAACCATATCCGCCGGACCCAATACTACAT
GAGCGCCCTGGCCACCGCGCTGCAGGACCACCCCCGCTTCGCCCCTCTGCTGGATGAAGCTGCCATCACCATGCTGTTCA
AATCGGCACCGCTGCACGATATCGGCAAGATCGGCATTCCCGACTGTATCTTGCTGAAGCCTGGGCGCCTGACGCCGGAG
GAATTCGAGGTCATGAAGACCCACGCCGCCCTCGGCCGCAACGCCATTGAATCCGCCGAACGCTGGCTGGGAACCCGCCT
GGAATTCCTGACAATGGCCAAGGAAATCACCCATTACCACCACGAGCGGTGGGACGGCGACGGCTACCCCGAGGGCCTGT
CCGGCGACGACATCCCCGTTTCCGCCCGCCTGATGGCCGTCGCCGATGTCTACGACGCATTGATCAGCCGTCGGGTCTAC
AAGGAGCCCATCCCCCACGCCGAGGCGGTGGACATCATCATCCGGGACAAGGGAGGGCGTTTCGACCCCGATGTGGTCGA
TGCCTTCGTCGGCATCCAGGACGAGTTCCGAGCCATCGCCCGGCAATATGCCGATAGGCCCACTCCCTGA

Upstream 100 bases:

>100_bases
CCGACGAGCATTCCGATTCCGATGCGGACCTGAAGCGCAAGAAGGATCTGCTCTCCATTTCGGGAACGGAAATGCCCGCT
CACCAGAAGGCGACCTCGTG

Downstream 100 bases:

>100_bases
GCCTCACGGCCGCCTTGTCCGGCGAGTAAAGGAACAAGGCCAGCCATCCCCTGAAGATGACGGGCCTTCCGTTCCGGCGG
TGGGGCCGCCCCGTCCTCAG

Product: response regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 369; Mature: 369

Protein sequence:

>369_residues
MLPADFMDRATILVVDDSPDDVWLMSELLKADYRVKVANSGERGLKIAMGNERPDLVLLDVMMPDIDGYEVCRQLKTDSR
THDIPVIFLTSKTRMAEEKRGLDMGAVDYITKPFSPSIVLARVKAHLRLKSSADFLRDQNRFLEREVAKRTKDVTAVKDV
TILAMASLAETRDLMTGNHIRRTQYYMSALATALQDHPRFAPLLDEAAITMLFKSAPLHDIGKIGIPDCILLKPGRLTPE
EFEVMKTHAALGRNAIESAERWLGTRLEFLTMAKEITHYHHERWDGDGYPEGLSGDDIPVSARLMAVADVYDALISRRVY
KEPIPHAEAVDIIIRDKGGRFDPDVVDAFVGIQDEFRAIARQYADRPTP

Sequences:

>Translated_369_residues
MLPADFMDRATILVVDDSPDDVWLMSELLKADYRVKVANSGERGLKIAMGNERPDLVLLDVMMPDIDGYEVCRQLKTDSR
THDIPVIFLTSKTRMAEEKRGLDMGAVDYITKPFSPSIVLARVKAHLRLKSSADFLRDQNRFLEREVAKRTKDVTAVKDV
TILAMASLAETRDLMTGNHIRRTQYYMSALATALQDHPRFAPLLDEAAITMLFKSAPLHDIGKIGIPDCILLKPGRLTPE
EFEVMKTHAALGRNAIESAERWLGTRLEFLTMAKEITHYHHERWDGDGYPEGLSGDDIPVSARLMAVADVYDALISRRVY
KEPIPHAEAVDIIIRDKGGRFDPDVVDAFVGIQDEFRAIARQYADRPTP
>Mature_369_residues
MLPADFMDRATILVVDDSPDDVWLMSELLKADYRVKVANSGERGLKIAMGNERPDLVLLDVMMPDIDGYEVCRQLKTDSR
THDIPVIFLTSKTRMAEEKRGLDMGAVDYITKPFSPSIVLARVKAHLRLKSSADFLRDQNRFLEREVAKRTKDVTAVKDV
TILAMASLAETRDLMTGNHIRRTQYYMSALATALQDHPRFAPLLDEAAITMLFKSAPLHDIGKIGIPDCILLKPGRLTPE
EFEVMKTHAALGRNAIESAERWLGTRLEFLTMAKEITHYHHERWDGDGYPEGLSGDDIPVSARLMAVADVYDALISRRVY
KEPIPHAEAVDIIIRDKGGRFDPDVVDAFVGIQDEFRAIARQYADRPTP

Specific function: Positively regulates the synthesis of extracellular enzymes and extracellular polysaccharides. The cell-cell signaling system encoded by rpf genes is required for full virulence and is implicated in regulation of the formation and dispersal of biofilms [H

COG id: COG3437

COG function: function code KT; Response regulator containing a CheY-like receiver domain and an HD-GYP domain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain [H]

Homologues:

Organism=Escherichia coli, GI87082012, Length=117, Percent_Identity=38.4615384615385, Blast_Score=85, Evalue=9e-18,
Organism=Escherichia coli, GI1788394, Length=130, Percent_Identity=37.6923076923077, Blast_Score=80, Evalue=2e-16,
Organism=Escherichia coli, GI1786599, Length=75, Percent_Identity=50.6666666666667, Blast_Score=79, Evalue=5e-16,
Organism=Escherichia coli, GI1786784, Length=117, Percent_Identity=34.1880341880342, Blast_Score=77, Evalue=1e-15,
Organism=Escherichia coli, GI1790860, Length=125, Percent_Identity=38.4, Blast_Score=77, Evalue=2e-15,
Organism=Escherichia coli, GI1789809, Length=122, Percent_Identity=35.2459016393443, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI1786911, Length=123, Percent_Identity=34.1463414634146, Blast_Score=65, Evalue=6e-12,
Organism=Escherichia coli, GI1787229, Length=117, Percent_Identity=35.8974358974359, Blast_Score=65, Evalue=9e-12,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011006
- InterPro:   IPR003607
- InterPro:   IPR001789 [H]

Pfam domain/function: PF00072 Response_reg [H]

EC number: NA

Molecular weight: Translated: 41575; Mature: 41575

Theoretical pI: Translated: 5.84; Mature: 5.84

Prosite motif: PS50110 RESPONSE_REGULATORY

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
4.1 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLPADFMDRATILVVDDSPDDVWLMSELLKADYRVKVANSGERGLKIAMGNERPDLVLLD
CCCCCCCCCEEEEEEECCCCCHHHHHHHHCCCCEEEEECCCCCCEEEEECCCCCCEEEEE
VMMPDIDGYEVCRQLKTDSRTHDIPVIFLTSKTRMAEEKRGLDMGAVDYITKPFSPSIVL
EECCCCCHHHHHHHHHCCCCCCCCEEEEEECCHHHHHHHCCCCCCHHHHHCCCCCCHHHH
ARVKAHLRLKSSADFLRDQNRFLEREVAKRTKDVTAVKDVTILAMASLAETRDLMTGNHI
HHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHH
RRTQYYMSALATALQDHPRFAPLLDEAAITMLFKSAPLHDIGKIGIPDCILLKPGRLTPE
HHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCHHHCCCCCCCEEEECCCCCCHH
EFEVMKTHAALGRNAIESAERWLGTRLEFLTMAKEITHYHHERWDGDGYPEGLSGDDIPV
HHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCH
SARLMAVADVYDALISRRVYKEPIPHAEAVDIIIRDKGGRFDPDVVDAFVGIQDEFRAIA
HHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEECCCCCCCHHHHHHHHCCHHHHHHHH
RQYADRPTP
HHHCCCCCC
>Mature Secondary Structure
MLPADFMDRATILVVDDSPDDVWLMSELLKADYRVKVANSGERGLKIAMGNERPDLVLLD
CCCCCCCCCEEEEEEECCCCCHHHHHHHHCCCCEEEEECCCCCCEEEEECCCCCCEEEEE
VMMPDIDGYEVCRQLKTDSRTHDIPVIFLTSKTRMAEEKRGLDMGAVDYITKPFSPSIVL
EECCCCCHHHHHHHHHCCCCCCCCEEEEEECCHHHHHHHCCCCCCHHHHHCCCCCCHHHH
ARVKAHLRLKSSADFLRDQNRFLEREVAKRTKDVTAVKDVTILAMASLAETRDLMTGNHI
HHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHH
RRTQYYMSALATALQDHPRFAPLLDEAAITMLFKSAPLHDIGKIGIPDCILLKPGRLTPE
HHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCHHHCCCCCCCEEEECCCCCCHH
EFEVMKTHAALGRNAIESAERWLGTRLEFLTMAKEITHYHHERWDGDGYPEGLSGDDIPV
HHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCH
SARLMAVADVYDALISRRVYKEPIPHAEAVDIIIRDKGGRFDPDVVDAFVGIQDEFRAIA
HHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEECCCCCCCHHHHHHHHCCHHHHHHHH
RQYADRPTP
HHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11123673 [H]