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The map label for this gene is rpfG [H]

Identifier: 83310939

GI number: 83310939

Start: 1977440

End: 1978621

Strand: Reverse

Name: rpfG [H]

Synonym: amb1840

Alternate gene names: 83310939

Gene position: 1978621-1977440 (Counterclockwise)

Preceding gene: 83310940

Following gene: 83310938

Centisome position: 39.83

GC content: 63.28

Gene sequence:

>1182_bases
GTGATGGACGCATCTGACGTCATCGAGAGAGCCACCATCCTGGTTGTGGACGACACGCCGGACAATCTGGCGGTGCTGAG
CAGCCTGCTCAAGGACGAGTACCGGGTGAAGGTGGCCAGCTCGGGCGAGAAGGCCCTTAAGGTCGCCATGGCCGAAACGT
CCCCCGACCTGATCCTGCTGGACATCATGATGCCGGGGCTGGACGGCTACGAGGTCTGCCGGCGCCTCAAGGCCGAACCC
AGGACGCACCATATTCCGGTCATCTTCCTGACCGCCAAAGCCGAGGTGGACGCCGAGAAGACGGGGCTGGACCTGGGTGC
CGTGGACTACATCACCAAGCCCATCAGCCCGCCCATCGTCCTTGCCCGGGTCAAGACCCACCTGAAACTCAAGGCCACCG
CCGATTTTCTCCGCGACAAGAACGACTTTCTGGAACACGAGGTCGCCAAGCGCACCGAGGAGGTGATGGCCATCCAGGAC
GTCACCATCCTGGCCATGGCGTCCCTGGCCGAAACCCGCGACCTGGAAACCGGCAACCACATCCGCCGCACCCAATTCTA
TGTCAAGGCCCTGGCCACCCGGCTGCGCACCCATCCCCGCTTCGCCGCCCATCTGGACGACGGCACCATCGATATGCTGT
TCAAATCGGCGCCGCTCCACGATATCGGCAAGGTGGGCATTCCCGACCGCATCCTGCTCAAGCCCGGGCGCTTCGTCCCG
GAAGAGTTCGAGATCATGAAGACTCACACCACGCTGGGCCGCGAGGCCATCGAACATGCCGAGCACTCCTTGGGAACCAA
GGTGGAGTTCCTGACCACCGCCAAGGAAATCGCCTTCGGCCACCAGGAGAAGTGGGACGGCAGCGGCTACCCCCAAGGGC
TGGCCGGCGACGACATTCCCCTGTCCGCCCGCCTGATGGCGGTGGCCGACGTTTACGACGCCCTGATCAGCCGGCGTGTC
TACAAGGAGGGCATGCCCCACGAAAAGGCGGTGGAAATCATCGTCCAGGGACGGGGCCAGCATTTCGACCCCGATATCGT
CGATGCCTTCCTGGCCATCCAGGACGAGTTCCGCGCCATCGCCGACGAGCATTCCGATTCCGATGCGGACCTGAAGCGCA
AGAAGGATCTGCTCTCCATTTCGGGAACGGAAATGCCCGCTCACCAGAAGGCGACCTCGTGA

Upstream 100 bases:

>100_bases
CCCCGCATCCGGGCAGCCATCAAGGATTTCAACTTCGATCAGGCCCTGGCCACCCTGACCGAGGCCGTGCAACGGAACCG
TGCCCCCGCCTGAGGAACCC

Downstream 100 bases:

>100_bases
TGCTGCCTGCGGATTTCATGGACAGGGCCACCATTCTGGTGGTGGACGACAGCCCCGATGACGTCTGGCTGATGAGCGAA
TTGCTCAAGGCCGATTACCG

Product: response regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 393; Mature: 393

Protein sequence:

>393_residues
MMDASDVIERATILVVDDTPDNLAVLSSLLKDEYRVKVASSGEKALKVAMAETSPDLILLDIMMPGLDGYEVCRRLKAEP
RTHHIPVIFLTAKAEVDAEKTGLDLGAVDYITKPISPPIVLARVKTHLKLKATADFLRDKNDFLEHEVAKRTEEVMAIQD
VTILAMASLAETRDLETGNHIRRTQFYVKALATRLRTHPRFAAHLDDGTIDMLFKSAPLHDIGKVGIPDRILLKPGRFVP
EEFEIMKTHTTLGREAIEHAEHSLGTKVEFLTTAKEIAFGHQEKWDGSGYPQGLAGDDIPLSARLMAVADVYDALISRRV
YKEGMPHEKAVEIIVQGRGQHFDPDIVDAFLAIQDEFRAIADEHSDSDADLKRKKDLLSISGTEMPAHQKATS

Sequences:

>Translated_393_residues
MMDASDVIERATILVVDDTPDNLAVLSSLLKDEYRVKVASSGEKALKVAMAETSPDLILLDIMMPGLDGYEVCRRLKAEP
RTHHIPVIFLTAKAEVDAEKTGLDLGAVDYITKPISPPIVLARVKTHLKLKATADFLRDKNDFLEHEVAKRTEEVMAIQD
VTILAMASLAETRDLETGNHIRRTQFYVKALATRLRTHPRFAAHLDDGTIDMLFKSAPLHDIGKVGIPDRILLKPGRFVP
EEFEIMKTHTTLGREAIEHAEHSLGTKVEFLTTAKEIAFGHQEKWDGSGYPQGLAGDDIPLSARLMAVADVYDALISRRV
YKEGMPHEKAVEIIVQGRGQHFDPDIVDAFLAIQDEFRAIADEHSDSDADLKRKKDLLSISGTEMPAHQKATS
>Mature_393_residues
MMDASDVIERATILVVDDTPDNLAVLSSLLKDEYRVKVASSGEKALKVAMAETSPDLILLDIMMPGLDGYEVCRRLKAEP
RTHHIPVIFLTAKAEVDAEKTGLDLGAVDYITKPISPPIVLARVKTHLKLKATADFLRDKNDFLEHEVAKRTEEVMAIQD
VTILAMASLAETRDLETGNHIRRTQFYVKALATRLRTHPRFAAHLDDGTIDMLFKSAPLHDIGKVGIPDRILLKPGRFVP
EEFEIMKTHTTLGREAIEHAEHSLGTKVEFLTTAKEIAFGHQEKWDGSGYPQGLAGDDIPLSARLMAVADVYDALISRRV
YKEGMPHEKAVEIIVQGRGQHFDPDIVDAFLAIQDEFRAIADEHSDSDADLKRKKDLLSISGTEMPAHQKATS

Specific function: Positively regulates the synthesis of extracellular enzymes and extracellular polysaccharides. The cell-cell signaling system encoded by rpf genes is required for full virulence and is implicated in regulation of the formation and dispersal of biofilms [H

COG id: COG3437

COG function: function code KT; Response regulator containing a CheY-like receiver domain and an HD-GYP domain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain [H]

Homologues:

Organism=Escherichia coli, GI1788394, Length=118, Percent_Identity=43.2203389830509, Blast_Score=83, Evalue=3e-17,
Organism=Escherichia coli, GI1789809, Length=126, Percent_Identity=37.3015873015873, Blast_Score=80, Evalue=3e-16,
Organism=Escherichia coli, GI1786599, Length=120, Percent_Identity=38.3333333333333, Blast_Score=79, Evalue=7e-16,
Organism=Escherichia coli, GI87082012, Length=142, Percent_Identity=34.5070422535211, Blast_Score=77, Evalue=2e-15,
Organism=Escherichia coli, GI1790860, Length=124, Percent_Identity=37.9032258064516, Blast_Score=75, Evalue=7e-15,
Organism=Escherichia coli, GI1789149, Length=106, Percent_Identity=39.622641509434, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI1786784, Length=118, Percent_Identity=33.8983050847458, Blast_Score=66, Evalue=4e-12,
Organism=Escherichia coli, GI1788550, Length=104, Percent_Identity=37.5, Blast_Score=63, Evalue=3e-11,
Organism=Escherichia coli, GI1790437, Length=104, Percent_Identity=37.5, Blast_Score=62, Evalue=6e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011006
- InterPro:   IPR003607
- InterPro:   IPR001789 [H]

Pfam domain/function: PF00072 Response_reg [H]

EC number: NA

Molecular weight: Translated: 43626; Mature: 43626

Theoretical pI: Translated: 5.71; Mature: 5.71

Prosite motif: PS50110 RESPONSE_REGULATORY

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMDASDVIERATILVVDDTPDNLAVLSSLLKDEYRVKVASSGEKALKVAMAETSPDLILL
CCCHHHHHCCEEEEEEECCCCHHHHHHHHHHHHHEEEEECCCCHHEEEEEECCCCCEEEE
DIMMPGLDGYEVCRRLKAEPRTHHIPVIFLTAKAEVDAEKTGLDLGAVDYITKPISPPIV
EECCCCCCHHHHHHHHHCCCCCCCCCEEEEEECCCCCHHHCCCCCCHHHHHHCCCCCCEE
LARVKTHLKLKATADFLRDKNDFLEHEVAKRTEEVMAIQDVTILAMASLAETRDLETGNH
EEEHHHHEEEHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
IRRTQFYVKALATRLRTHPRFAAHLDDGTIDMLFKSAPLHDIGKVGIPDRILLKPGRFVP
HHHHHHHHHHHHHHHHCCCCEEEECCCCHHHHHCCCCCCHHHCCCCCCCEEEECCCCCCC
EEFEIMKTHTTLGREAIEHAEHSLGTKVEFLTTAKEIAFGHQEKWDGSGYPQGLAGDDIP
HHHHHHHHHHHHHHHHHHHHHHHCCCEEHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCC
LSARLMAVADVYDALISRRVYKEGMPHEKAVEIIVQGRGQHFDPDIVDAFLAIQDEFRAI
HHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHEEEECCCCCCCHHHHHHHHHHHHHHHHH
ADEHSDSDADLKRKKDLLSISGTEMPAHQKATS
HHCCCCCCHHHHHHHHHHEECCCCCCCCCCCCC
>Mature Secondary Structure
MMDASDVIERATILVVDDTPDNLAVLSSLLKDEYRVKVASSGEKALKVAMAETSPDLILL
CCCHHHHHCCEEEEEEECCCCHHHHHHHHHHHHHEEEEECCCCHHEEEEEECCCCCEEEE
DIMMPGLDGYEVCRRLKAEPRTHHIPVIFLTAKAEVDAEKTGLDLGAVDYITKPISPPIV
EECCCCCCHHHHHHHHHCCCCCCCCCEEEEEECCCCCHHHCCCCCCHHHHHHCCCCCCEE
LARVKTHLKLKATADFLRDKNDFLEHEVAKRTEEVMAIQDVTILAMASLAETRDLETGNH
EEEHHHHEEEHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
IRRTQFYVKALATRLRTHPRFAAHLDDGTIDMLFKSAPLHDIGKVGIPDRILLKPGRFVP
HHHHHHHHHHHHHHHHCCCCEEEECCCCHHHHHCCCCCCHHHCCCCCCCEEEECCCCCCC
EEFEIMKTHTTLGREAIEHAEHSLGTKVEFLTTAKEIAFGHQEKWDGSGYPQGLAGDDIP
HHHHHHHHHHHHHHHHHHHHHHHCCCEEHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCC
LSARLMAVADVYDALISRRVYKEGMPHEKAVEIIVQGRGQHFDPDIVDAFLAIQDEFRAI
HHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHEEEECCCCCCCHHHHHHHHHHHHHHHHH
ADEHSDSDADLKRKKDLLSISGTEMPAHQKATS
HHCCCCCCHHHHHHHHHHEECCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11123673 [H]