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The map label for this gene is nifD1 [H]

Identifier: 83310672

GI number: 83310672

Start: 1702403

End: 1703860

Strand: Reverse

Name: nifD1 [H]

Synonym: amb1573

Alternate gene names: 83310672

Gene position: 1703860-1702403 (Counterclockwise)

Preceding gene: 83310673

Following gene: 83310671

Centisome position: 34.3

GC content: 62.41

Gene sequence:

>1458_bases
ATGAGCCTCGAATACACCAACGACACCGCCGCCAACCAGGCGCTGATCAAGGAAGTCCTCGATCAGTACCCGGAAAAGTC
GGCCAAGAAGCGCGCCCGCCACCTCGGCACCGTCAAGACCGAAGAGAACGGCACCCCGACCTGCGGCGTGAAGTCCAACA
TCAAGTCGGTTCCCGGCGTCATGACCGTTCGCGGTTGCGCCTATGCCGGCGCCAAGGGCGTGGTGTGGGGTCCGGTCAAG
GACATGGTCCACATCTCCCACGGTCCGATCGGCTGCGGTCAGTATTCCTGGTCCCAGCGTCGCAACTACTTCACCGGCAC
CGTCGGCATCGACAGCTTCGTCACCATGCAGGTGACCTCGGATTTCCAGGAACGCGACATCGTTTTCGGCGGTGACAAGA
ACCTGGAAGTGATGATCGACGAGCTGGACGGGCTGTTCCCGCTGAACAACGGCATCTCGGTGCTGTCCGAGTGCCCCATC
GGCCTGATCGGCGACGACATTGAAGCCGTCGCCAAGAAGAAGGCCAAGGACATCGGCAAGAAGGTCGTTCCGGTCCGCTG
CGAAGGCTTCCGTGGCGTGTCCCAGTCCCTGGGCCACCACATCGCCAACGACGCCATCCGGGATTGGGTCTTCGAGAAGG
GCACCGAGGAAGAGTGGGCGACCGGCCCCTACGACGTGAACCTGATCGCCGACTACAACATCGGCGGCGATGCCTGGCCG
AGCCGCAAGCTCCTGGAAGAGATCGGCCTGCGGGTCATCGGTTCCTGGTCCGGCGACGCCACCCTGGCCGAGATCGAGCG
CGCCCCCAAGGCCAAGCTGAACCTCATCCATTGCTATCGGTCGATGAACTACATCTGCCGTCACATGGAAGAGAAGTACG
GCATGCCCTGGATCGAATACAACTTCTTCGGTCCGACCCAGATCGCCGCTTCGCTCCGCAAGATCGCCGCCGCCTTCGAC
GAGACCATCCAGAAGAAGGCCGAAGAGGTCATCGCCAAGTATCAGCCACTGGTGGACGCGGTCACCGCCCGCTTCAAGCC
GCGGCTGGATGGCAAGACGGTCATGCTGTATGTGGGCGGCCTGCGCCCCCGTCACACCGCCACCGCCTACGAAGACCTGG
GCATGAAGATCGTGGGCTCGGGCTACGAGTTCGCTCACAACGACGACTATCAGCGCACCGCCACCTACCTCAAGGAAGGC
ACGCTGATTTATGACGACGTCAGCGAGTTCGAGCTGGAGAAGTTCATCGAGCAGCTCCGTCCCGACCTGGTCGGTTCGGG
CATCAAGGAGAAGTACTCGACCCAGAAGATGGGCGTGCCCTTCCGCCAGATGCACTCGTGGGACTACTCGGGCCCCTATC
ACGGCTATGACGGCTTCGCCATCTTCGCCCGTGACATGGACATGGCCATCAACGCCCCGGTGTGGGGCAAGCTCAAGGCC
CCGTGGAAGGCCGCCTAA

Upstream 100 bases:

>100_bases
CCGGTTGATCGGCATCAGCGCCTAACCCGCCCCCGCCGGCCCGGTTCACGGGTCGGCGGACCGGGCGGGCAAAGTGAAGC
TCTAGAGAAGGAGAAGGGGC

Downstream 100 bases:

>100_bases
GCCCTTTCACACGGACCCTGATCTGGTTTTAGGAAATCCCCTCCCCGCGTGTCGCCCAATATCCGGGGGCGCGGGGAGGA
ATAAGGAGAGACTGCCATGC

Product: nitrogenase molybdenum-iron protein alpha chain

Products: NA

Alternate protein names: Dinitrogenase; Nitrogenase component I [H]

Number of amino acids: Translated: 485; Mature: 484

Protein sequence:

>485_residues
MSLEYTNDTAANQALIKEVLDQYPEKSAKKRARHLGTVKTEENGTPTCGVKSNIKSVPGVMTVRGCAYAGAKGVVWGPVK
DMVHISHGPIGCGQYSWSQRRNYFTGTVGIDSFVTMQVTSDFQERDIVFGGDKNLEVMIDELDGLFPLNNGISVLSECPI
GLIGDDIEAVAKKKAKDIGKKVVPVRCEGFRGVSQSLGHHIANDAIRDWVFEKGTEEEWATGPYDVNLIADYNIGGDAWP
SRKLLEEIGLRVIGSWSGDATLAEIERAPKAKLNLIHCYRSMNYICRHMEEKYGMPWIEYNFFGPTQIAASLRKIAAAFD
ETIQKKAEEVIAKYQPLVDAVTARFKPRLDGKTVMLYVGGLRPRHTATAYEDLGMKIVGSGYEFAHNDDYQRTATYLKEG
TLIYDDVSEFELEKFIEQLRPDLVGSGIKEKYSTQKMGVPFRQMHSWDYSGPYHGYDGFAIFARDMDMAINAPVWGKLKA
PWKAA

Sequences:

>Translated_485_residues
MSLEYTNDTAANQALIKEVLDQYPEKSAKKRARHLGTVKTEENGTPTCGVKSNIKSVPGVMTVRGCAYAGAKGVVWGPVK
DMVHISHGPIGCGQYSWSQRRNYFTGTVGIDSFVTMQVTSDFQERDIVFGGDKNLEVMIDELDGLFPLNNGISVLSECPI
GLIGDDIEAVAKKKAKDIGKKVVPVRCEGFRGVSQSLGHHIANDAIRDWVFEKGTEEEWATGPYDVNLIADYNIGGDAWP
SRKLLEEIGLRVIGSWSGDATLAEIERAPKAKLNLIHCYRSMNYICRHMEEKYGMPWIEYNFFGPTQIAASLRKIAAAFD
ETIQKKAEEVIAKYQPLVDAVTARFKPRLDGKTVMLYVGGLRPRHTATAYEDLGMKIVGSGYEFAHNDDYQRTATYLKEG
TLIYDDVSEFELEKFIEQLRPDLVGSGIKEKYSTQKMGVPFRQMHSWDYSGPYHGYDGFAIFARDMDMAINAPVWGKLKA
PWKAA
>Mature_484_residues
SLEYTNDTAANQALIKEVLDQYPEKSAKKRARHLGTVKTEENGTPTCGVKSNIKSVPGVMTVRGCAYAGAKGVVWGPVKD
MVHISHGPIGCGQYSWSQRRNYFTGTVGIDSFVTMQVTSDFQERDIVFGGDKNLEVMIDELDGLFPLNNGISVLSECPIG
LIGDDIEAVAKKKAKDIGKKVVPVRCEGFRGVSQSLGHHIANDAIRDWVFEKGTEEEWATGPYDVNLIADYNIGGDAWPS
RKLLEEIGLRVIGSWSGDATLAEIERAPKAKLNLIHCYRSMNYICRHMEEKYGMPWIEYNFFGPTQIAASLRKIAAAFDE
TIQKKAEEVIAKYQPLVDAVTARFKPRLDGKTVMLYVGGLRPRHTATAYEDLGMKIVGSGYEFAHNDDYQRTATYLKEGT
LIYDDVSEFELEKFIEQLRPDLVGSGIKEKYSTQKMGVPFRQMHSWDYSGPYHGYDGFAIFARDMDMAINAPVWGKLKAP
WKAA

Specific function: This molybdenum-iron protein is part of the nitrogenase complex that catalyzes the key enzymatic reactions in nitrogen fixation [H]

COG id: COG2710

COG function: function code C; Nitrogenase molybdenum-iron protein, alpha and beta chains

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the nifD/nifK/nifE/nifN family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000510
- InterPro:   IPR010143
- InterPro:   IPR000318
- InterPro:   IPR005972 [H]

Pfam domain/function: PF00148 Oxidored_nitro [H]

EC number: =1.18.6.1 [H]

Molecular weight: Translated: 54104; Mature: 53973

Theoretical pI: Translated: 6.59; Mature: 6.59

Prosite motif: PS00699 NITROGENASE_1_1 ; PS00090 NITROGENASE_1_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSLEYTNDTAANQALIKEVLDQYPEKSAKKRARHLGTVKTEENGTPTCGVKSNIKSVPGV
CCCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCEECCCCCCCCCCCCCHHHCCCC
MTVRGCAYAGAKGVVWGPVKDMVHISHGPIGCGQYSWSQRRNYFTGTVGIDSFVTMQVTS
EEECHHHHCCCCCCEECCHHHHEEECCCCCCCCCCCHHHCCCEEEEEECCCCEEEEEECC
DFQERDIVFGGDKNLEVMIDELDGLFPLNNGISVLSECPIGLIGDDIEAVAKKKAKDIGK
CCCCCCEEECCCCCCEEEEEHHCCCEECCCCHHHHHHCCCCCCCCCHHHHHHHHHHHHCC
KVVPVRCEGFRGVSQSLGHHIANDAIRDWVFEKGTEEEWATGPYDVNLIADYNIGGDAWP
CEEEEEECCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEEEECCCCCCCCC
SRKLLEEIGLRVIGSWSGDATLAEIERAPKAKLNLIHCYRSMNYICRHMEEKYGMPWIEY
HHHHHHHCCCEEEECCCCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCEEEE
NFFGPTQIAASLRKIAAAFDETIQKKAEEVIAKYQPLVDAVTARFKPRLDGKTVMLYVGG
ECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEECC
LRPRHTATAYEDLGMKIVGSGYEFAHNDDYQRTATYLKEGTLIYDDVSEFELEKFIEQLR
CCCCCCHHHHHHCCCEEEECCCCCCCCCCHHHHHHHHHCCCEEECCHHHHHHHHHHHHHC
PDLVGSGIKEKYSTQKMGVPFRQMHSWDYSGPYHGYDGFAIFARDMDMAINAPVWGKLKA
HHHHCCCHHHHHHHHHCCCCHHHHHCCCCCCCCCCCCCEEEEEECCCCEECCCCCCCCCC
PWKAA
CCCCC
>Mature Secondary Structure 
SLEYTNDTAANQALIKEVLDQYPEKSAKKRARHLGTVKTEENGTPTCGVKSNIKSVPGV
CCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCEECCCCCCCCCCCCCHHHCCCC
MTVRGCAYAGAKGVVWGPVKDMVHISHGPIGCGQYSWSQRRNYFTGTVGIDSFVTMQVTS
EEECHHHHCCCCCCEECCHHHHEEECCCCCCCCCCCHHHCCCEEEEEECCCCEEEEEECC
DFQERDIVFGGDKNLEVMIDELDGLFPLNNGISVLSECPIGLIGDDIEAVAKKKAKDIGK
CCCCCCEEECCCCCCEEEEEHHCCCEECCCCHHHHHHCCCCCCCCCHHHHHHHHHHHHCC
KVVPVRCEGFRGVSQSLGHHIANDAIRDWVFEKGTEEEWATGPYDVNLIADYNIGGDAWP
CEEEEEECCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEEEECCCCCCCCC
SRKLLEEIGLRVIGSWSGDATLAEIERAPKAKLNLIHCYRSMNYICRHMEEKYGMPWIEY
HHHHHHHCCCEEEECCCCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCEEEE
NFFGPTQIAASLRKIAAAFDETIQKKAEEVIAKYQPLVDAVTARFKPRLDGKTVMLYVGG
ECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEECC
LRPRHTATAYEDLGMKIVGSGYEFAHNDDYQRTATYLKEGTLIYDDVSEFELEKFIEQLR
CCCCCCHHHHHHCCCEEEECCCCCCCCCCHHHHHHHHHCCCEEECCHHHHHHHHHHHHHC
PDLVGSGIKEKYSTQKMGVPFRQMHSWDYSGPYHGYDGFAIFARDMDMAINAPVWGKLKA
HHHHCCCHHHHHHHHHCCCCHHHHHCCCCCCCCCCCCCEEEEEECCCCEECCCCCCCCCC
PWKAA
CCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9163424; 2744485 [H]