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The map label for this gene is nifH

Identifier: 83310673

GI number: 83310673

Start: 1703953

End: 1704840

Strand: Reverse

Name: nifH

Synonym: amb1574

Alternate gene names: 83310673

Gene position: 1704840-1703953 (Counterclockwise)

Preceding gene: 83310677

Following gene: 83310672

Centisome position: 34.32

GC content: 64.3

Gene sequence:

>888_bases
ATGGTTCGTCAGATCGCTTTCTACGGCAAGGGCGGTATCGGTAAGTCCACCACCTCGCAGAACACCCTGGCCGCTCTCGT
CGAGATGGGTCAGAAGATCCTCATCGTCGGCTGCGACCCCAAGGCCGACTCCACCCGCCTGATCCTCAACACCAAGCTGC
AGGACACCGTGCTGCACCTGGCCGCCAAGGCCGGCTCGGTGGAAGACCTCGAGCTCGAGGACGTGATGAAGATCGGCTAC
AAGGGCATCAAGTGCACCGAGTCGGGCGGCCCCGAGCCGGGCGTCGGCTGCGCCGGCCGTGGCGTGATCACCGCCATCAA
CTTCCTGGAAGAGAACGGCGCCTATGACGACGTGGACTACGTGTCCTACGACGTGCTGGGCGACGTGGTGTGCGGCGGCT
TCGCCATGCCGATCCGCGAGAACAAGGCTCAGGAAATCTACATCGTCATGTCCGGCGAGATGATGGCCCTGTTCGCCGCC
AACAACATCGCCAAGGGCATTCTGAAGTATGCCGGTTCGGGCGGCGTCCGCCTGGGCGGCCTGATCTGCAACGAGCGCCA
GACCGACCGCGAGCTGGAGCTGGCCGAGGCCCTGGCCAAGAAGCTGAACACCCAGATGATCCACTTCGTGCCGCGTGACA
ACGGCGTGCAGCACGCCGAGCTGCGTCGCCAGACCGTCATCCAGTACGCCCCCGACTCCAAGCAGGCCGGCGAATACCGC
GCCCTGGCCACCAAGATCCACAACAACGGCGGCAAGGGCACCATCCCGACCCCGATCACCATGGAAGAGCTCGAGGACAT
GCTGCTCGAGTTCGGCATCATGAAGTCCGACGAGCAGGCCCTGGCCGAGCTCGAGGCCAAGGAAAAGACCCTCTGCAGCG
CCGGTTGA

Upstream 100 bases:

>100_bases
GCGCACACCAAACTGGCACGCCCATTGCGATGAGGAGGCCGTGGCGGTGACCCAGTCCTCGCCGAGCAAATTTTGAAGGC
CAACCTGAAAGGAAATTCCC

Downstream 100 bases:

>100_bases
TCGGCATCAGCGCCTAACCCGCCCCCGCCGGCCCGGTTCACGGGTCGGCGGACCGGGCGGGCAAAGTGAAGCTCTAGAGA
AGGAGAAGGGGCATGAGCCT

Product: nitrogenase reductase

Products: NA

Alternate protein names: Nitrogenase Fe protein; Nitrogenase component II; Nitrogenase reductase [H]

Number of amino acids: Translated: 295; Mature: 295

Protein sequence:

>295_residues
MVRQIAFYGKGGIGKSTTSQNTLAALVEMGQKILIVGCDPKADSTRLILNTKLQDTVLHLAAKAGSVEDLELEDVMKIGY
KGIKCTESGGPEPGVGCAGRGVITAINFLEENGAYDDVDYVSYDVLGDVVCGGFAMPIRENKAQEIYIVMSGEMMALFAA
NNIAKGILKYAGSGGVRLGGLICNERQTDRELELAEALAKKLNTQMIHFVPRDNGVQHAELRRQTVIQYAPDSKQAGEYR
ALATKIHNNGGKGTIPTPITMEELEDMLLEFGIMKSDEQALAELEAKEKTLCSAG

Sequences:

>Translated_295_residues
MVRQIAFYGKGGIGKSTTSQNTLAALVEMGQKILIVGCDPKADSTRLILNTKLQDTVLHLAAKAGSVEDLELEDVMKIGY
KGIKCTESGGPEPGVGCAGRGVITAINFLEENGAYDDVDYVSYDVLGDVVCGGFAMPIRENKAQEIYIVMSGEMMALFAA
NNIAKGILKYAGSGGVRLGGLICNERQTDRELELAEALAKKLNTQMIHFVPRDNGVQHAELRRQTVIQYAPDSKQAGEYR
ALATKIHNNGGKGTIPTPITMEELEDMLLEFGIMKSDEQALAELEAKEKTLCSAG
>Mature_295_residues
MVRQIAFYGKGGIGKSTTSQNTLAALVEMGQKILIVGCDPKADSTRLILNTKLQDTVLHLAAKAGSVEDLELEDVMKIGY
KGIKCTESGGPEPGVGCAGRGVITAINFLEENGAYDDVDYVSYDVLGDVVCGGFAMPIRENKAQEIYIVMSGEMMALFAA
NNIAKGILKYAGSGGVRLGGLICNERQTDRELELAEALAKKLNTQMIHFVPRDNGVQHAELRRQTVIQYAPDSKQAGEYR
ALATKIHNNGGKGTIPTPITMEELEDMLLEFGIMKSDEQALAELEAKEKTLCSAG

Specific function: The key enzymatic reactions in nitrogen fixation are catalyzed by the nitrogenase complex, which has 2 components:the iron protein and the molybdenum-iron protein [H]

COG id: COG1348

COG function: function code P; Nitrogenase subunit NifH (ATPase)

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the NifH/BchL/ChlL family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000392
- InterPro:   IPR005977 [H]

Pfam domain/function: PF00142 Fer4_NifH [H]

EC number: =1.18.6.1 [H]

Molecular weight: Translated: 31749; Mature: 31749

Theoretical pI: Translated: 4.87; Mature: 4.87

Prosite motif: PS00746 NIFH_FRXC_1 ; PS00692 NIFH_FRXC_2 ; PS51026 NIFH_FRXC_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
5.8 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
5.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVRQIAFYGKGGIGKSTTSQNTLAALVEMGQKILIVGCDPKADSTRLILNTKLQDTVLHL
CCCEEEEECCCCCCCCCCCHHHHHHHHHCCCEEEEEECCCCCCCEEEEEECCHHHHHHHH
AAKAGSVEDLELEDVMKIGYKGIKCTESGGPEPGVGCAGRGVITAINFLEENGAYDDVDY
HHHCCCCCCCCHHHHHHHCCCCEEEECCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCHHH
VSYDVLGDVVCGGFAMPIRENKAQEIYIVMSGEMMALFAANNIAKGILKYAGSGGVRLGG
HHHHHHHHHHHCCEECCCCCCCCCEEEEEECCCEEEEEHHHHHHHHHHHHCCCCCCEECC
LICNERQTDRELELAEALAKKLNTQMIHFVPRDNGVQHAELRRQTVIQYAPDSKQAGEYR
EEECCCCCCHHHHHHHHHHHHCCCEEEEEEECCCCCCHHHHHHHHHEEECCCCCCCCHHH
ALATKIHNNGGKGTIPTPITMEELEDMLLEFGIMKSDEQALAELEAKEKTLCSAG
HHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MVRQIAFYGKGGIGKSTTSQNTLAALVEMGQKILIVGCDPKADSTRLILNTKLQDTVLHL
CCCEEEEECCCCCCCCCCCHHHHHHHHHCCCEEEEEECCCCCCCEEEEEECCHHHHHHHH
AAKAGSVEDLELEDVMKIGYKGIKCTESGGPEPGVGCAGRGVITAINFLEENGAYDDVDY
HHHCCCCCCCCHHHHHHHCCCCEEEECCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCHHH
VSYDVLGDVVCGGFAMPIRENKAQEIYIVMSGEMMALFAANNIAKGILKYAGSGGVRLGG
HHHHHHHHHHHCCEECCCCCCCCCEEEEEECCCEEEEEHHHHHHHHHHHHCCCCCCEECC
LICNERQTDRELELAEALAKKLNTQMIHFVPRDNGVQHAELRRQTVIQYAPDSKQAGEYR
EEECCCCCCHHHHHHHHHHHHCCCEEEEEEECCCCCCHHHHHHHHHEEECCCCCCCCHHH
ALATKIHNNGGKGTIPTPITMEELEDMLLEFGIMKSDEQALAELEAKEKTLCSAG
HHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1979299 [H]