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The map label for this gene is nifK1 [H]

Identifier: 83310671

GI number: 83310671

Start: 1700771

End: 1702306

Strand: Reverse

Name: nifK1 [H]

Synonym: amb1572

Alternate gene names: 83310671

Gene position: 1702306-1700771 (Counterclockwise)

Preceding gene: 83310672

Following gene: 83310669

Centisome position: 34.27

GC content: 63.28

Gene sequence:

>1536_bases
ATGCCGCAGAGCGCTGAAAACATCAAGGACCACATCTCCCTGTTCTCCGAGCCCGAGTACAAGGAATTGTTGGCCCAGAA
GAAGGCCAATTACGAGTGCCGCCCGTCTGACGAAAAGGTCAACGAGCAGCTCGAATACTCCAAGACCTGGGAGTATCGCG
AGAAGAACTTCGCCCGTACCAAGGCCGTCATCAACCCGCACAAGGCCTGTCAGCCGCTCGGTGCCGTGTTCGCCGCCGCC
GGCTTCGAAGAGACCCTGCCCTACGTGCATGGCTCCCAGGGCTGCGCCGCCTACTTCCGCTCGCACCTCGCCCGTCACTT
CAAGGAAGCTTCGGCCTGCGTGTCGGACTCCATGACCGAAGACGCGGCCGTGTTCGGCGGCCTGAACAACCTGGTGGAGG
GCCTGCTCAACGCCAAGCACCTCTACAAGCCGAAGATGATCGCCGTCTCCACCACCTGCATGGCGGAAGTCATCGGCGAC
GATCTCAGCGCCTTCATCGCCACCGCCAAGGAAAAGGAATCGGTCCCGGCCGACTTCCCCGTCCCCTTCGCCCACACTCC
GTCGTTCGTCGGCAGCCACACCGTCGGCTACGACAACATGATCAAGGGCATCTTCAACTACTTCTGGGACCGCGAGAAGG
GCGAGGTCAAGACCGGCGCGTCCAACGGCAAGATCAACATCATCCCCGGCTTCGACGGTTACGCGGTCGCCAACAACCGC
GAACTGAAGCGCTACCTGGATGCCATGGGCGTCGATTACACCCTGGTCGGCGACGTCTCGGAGGTGTTCGACACCCCCAC
CGATGGCACCTTCCGCATGTATGACGGCGGCACCAAGCTCGATGACGTCCGCGACGCCATCAACGCCAAGGCCACCCTCG
GCCTGCAGCACTACTGCTCGGACAAGACCCTGGAATACGTCGCGTCCAAGGGTCAGGCCACCGCGTCCCTGAACTACCCC
ATGGGCGTGTCCGGGACCGACGCCATGCTGATGAAGATCTCCGAGCTGTCGGGCAAGGCGATCCCCGAGGCCATCGAAAA
GGAACGCGGCCGTCTGGTCGACGCCATCGCCGACAGCCAGGGCTGGCTGCACGGCAAGAAGGCCGCTATCTTCGGCGATC
CCGACTTCGTGCTGTCCATGGCCTCCTTCCTGATGGAGCTGGGCATTGAACCCACTCACTGCCTGTGCACCAACGCCGGC
AAGGAATGGGACGCCCAGATGAAGGCCGTCCTGGCCGCGTCGCCCTTCGGCGCCGATTGCCAGGTGTGGGCGGGCAAGGA
CCTCTGGCACCTGCGCTCCCTGCTCTTCACCGAGCCGACCGACATGATCATCGGCAGCTCCTACGGCAAGTATCTGGAGA
AGGACACCGGCATCCCCCTGATCCGTCTGACCTTCCCCGTGTTCGACCGTCACCACCACCACCGCTTCCCCACCATGGGC
TACCAGGGTGCGCTGCGGACCCTGGTCGAGATCCTCGACCGGATCTTCGAGGTGGCCGACGCGTCGAGCGACATCTCCTA
CGACCTCACGCGTTGA

Upstream 100 bases:

>100_bases
CTAAGCCCTTTCACACGGACCCTGATCTGGTTTTAGGAAATCCCCTCCCCGCGTGTCGCCCAATATCCGGGGGCGCGGGG
AGGAATAAGGAGAGACTGCC

Downstream 100 bases:

>100_bases
TCTCCCAACCCTTCCATAACGCGTGAGACCCGCCCCGGAGAGGAACCCGCCCTCTCCGGGGCTTCTCGTTTGGGGGATGA
GTTGCGAAGGGTGCCGCCCT

Product: nitrogenase molybdenum-iron protein alpha and beta chains

Products: NA

Alternate protein names: Dinitrogenase; Nitrogenase component I [H]

Number of amino acids: Translated: 511; Mature: 510

Protein sequence:

>511_residues
MPQSAENIKDHISLFSEPEYKELLAQKKANYECRPSDEKVNEQLEYSKTWEYREKNFARTKAVINPHKACQPLGAVFAAA
GFEETLPYVHGSQGCAAYFRSHLARHFKEASACVSDSMTEDAAVFGGLNNLVEGLLNAKHLYKPKMIAVSTTCMAEVIGD
DLSAFIATAKEKESVPADFPVPFAHTPSFVGSHTVGYDNMIKGIFNYFWDREKGEVKTGASNGKINIIPGFDGYAVANNR
ELKRYLDAMGVDYTLVGDVSEVFDTPTDGTFRMYDGGTKLDDVRDAINAKATLGLQHYCSDKTLEYVASKGQATASLNYP
MGVSGTDAMLMKISELSGKAIPEAIEKERGRLVDAIADSQGWLHGKKAAIFGDPDFVLSMASFLMELGIEPTHCLCTNAG
KEWDAQMKAVLAASPFGADCQVWAGKDLWHLRSLLFTEPTDMIIGSSYGKYLEKDTGIPLIRLTFPVFDRHHHHRFPTMG
YQGALRTLVEILDRIFEVADASSDISYDLTR

Sequences:

>Translated_511_residues
MPQSAENIKDHISLFSEPEYKELLAQKKANYECRPSDEKVNEQLEYSKTWEYREKNFARTKAVINPHKACQPLGAVFAAA
GFEETLPYVHGSQGCAAYFRSHLARHFKEASACVSDSMTEDAAVFGGLNNLVEGLLNAKHLYKPKMIAVSTTCMAEVIGD
DLSAFIATAKEKESVPADFPVPFAHTPSFVGSHTVGYDNMIKGIFNYFWDREKGEVKTGASNGKINIIPGFDGYAVANNR
ELKRYLDAMGVDYTLVGDVSEVFDTPTDGTFRMYDGGTKLDDVRDAINAKATLGLQHYCSDKTLEYVASKGQATASLNYP
MGVSGTDAMLMKISELSGKAIPEAIEKERGRLVDAIADSQGWLHGKKAAIFGDPDFVLSMASFLMELGIEPTHCLCTNAG
KEWDAQMKAVLAASPFGADCQVWAGKDLWHLRSLLFTEPTDMIIGSSYGKYLEKDTGIPLIRLTFPVFDRHHHHRFPTMG
YQGALRTLVEILDRIFEVADASSDISYDLTR
>Mature_510_residues
PQSAENIKDHISLFSEPEYKELLAQKKANYECRPSDEKVNEQLEYSKTWEYREKNFARTKAVINPHKACQPLGAVFAAAG
FEETLPYVHGSQGCAAYFRSHLARHFKEASACVSDSMTEDAAVFGGLNNLVEGLLNAKHLYKPKMIAVSTTCMAEVIGDD
LSAFIATAKEKESVPADFPVPFAHTPSFVGSHTVGYDNMIKGIFNYFWDREKGEVKTGASNGKINIIPGFDGYAVANNRE
LKRYLDAMGVDYTLVGDVSEVFDTPTDGTFRMYDGGTKLDDVRDAINAKATLGLQHYCSDKTLEYVASKGQATASLNYPM
GVSGTDAMLMKISELSGKAIPEAIEKERGRLVDAIADSQGWLHGKKAAIFGDPDFVLSMASFLMELGIEPTHCLCTNAGK
EWDAQMKAVLAASPFGADCQVWAGKDLWHLRSLLFTEPTDMIIGSSYGKYLEKDTGIPLIRLTFPVFDRHHHHRFPTMGY
QGALRTLVEILDRIFEVADASSDISYDLTR

Specific function: This molybdenum-iron protein is part of the nitrogenase complex that catalyzes the key enzymatic reactions in nitrogen fixation [H]

COG id: COG2710

COG function: function code C; Nitrogenase molybdenum-iron protein, alpha and beta chains

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the nifD/nifK/nifE/nifN family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000510
- InterPro:   IPR000318
- InterPro:   IPR005976 [H]

Pfam domain/function: PF00148 Oxidored_nitro [H]

EC number: =1.18.6.1 [H]

Molecular weight: Translated: 56438; Mature: 56307

Theoretical pI: Translated: 5.59; Mature: 5.59

Prosite motif: PS00699 NITROGENASE_1_1 ; PS00090 NITROGENASE_1_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPQSAENIKDHISLFSEPEYKELLAQKKANYECRPSDEKVNEQLEYSKTWEYREKNFART
CCCCHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
KAVINPHKACQPLGAVFAAAGFEETLPYVHGSQGCAAYFRSHLARHFKEASACVSDSMTE
HHHCCCHHHHHHHHHHHHHCCHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCH
DAAVFGGLNNLVEGLLNAKHLYKPKMIAVSTTCMAEVIGDDLSAFIATAKEKESVPADFP
HHHHHCCHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
VPFAHTPSFVGSHTVGYDNMIKGIFNYFWDREKGEVKTGASNGKINIIPGFDGYAVANNR
CCCCCCCCHHCCCCCCHHHHHHHHHHHHCCCCCCCEEECCCCCEEEEEECCCCEEECCCH
ELKRYLDAMGVDYTLVGDVSEVFDTPTDGTFRMYDGGTKLDDVRDAINAKATLGLQHYCS
HHHHHHHHHCCCEEEECCHHHHHCCCCCCEEEEECCCCCHHHHHHHHCCHHHHCHHHHCC
DKTLEYVASKGQATASLNYPMGVSGTDAMLMKISELSGKAIPEAIEKERGRLVDAIADSQ
CHHHHHHHCCCCCEEEECCCCCCCCCHHHHHHHHHHCCCCCHHHHHHHHCCEEHHHHCCC
GWLHGKKAAIFGDPDFVLSMASFLMELGIEPTHCLCTNAGKEWDAQMKAVLAASPFGADC
CCCCCCCEEEECCHHHHHHHHHHHHHHCCCCCEEEECCCCCHHHHHHHHHHHCCCCCCCC
QVWAGKDLWHLRSLLFTEPTDMIIGSSYGKYLEKDTGIPLIRLTFPVFDRHHHHRFPTMG
EEECCCCHHHHHHHHHCCCCHHEECCHHHHHHHHCCCCCEEEEECHHHHCCCCCCCCCCC
YQGALRTLVEILDRIFEVADASSDISYDLTR
HHHHHHHHHHHHHHHHHHHCCCCCCCCCCCC
>Mature Secondary Structure 
PQSAENIKDHISLFSEPEYKELLAQKKANYECRPSDEKVNEQLEYSKTWEYREKNFART
CCCHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
KAVINPHKACQPLGAVFAAAGFEETLPYVHGSQGCAAYFRSHLARHFKEASACVSDSMTE
HHHCCCHHHHHHHHHHHHHCCHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCH
DAAVFGGLNNLVEGLLNAKHLYKPKMIAVSTTCMAEVIGDDLSAFIATAKEKESVPADFP
HHHHHCCHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
VPFAHTPSFVGSHTVGYDNMIKGIFNYFWDREKGEVKTGASNGKINIIPGFDGYAVANNR
CCCCCCCCHHCCCCCCHHHHHHHHHHHHCCCCCCCEEECCCCCEEEEEECCCCEEECCCH
ELKRYLDAMGVDYTLVGDVSEVFDTPTDGTFRMYDGGTKLDDVRDAINAKATLGLQHYCS
HHHHHHHHHCCCEEEECCHHHHHCCCCCCEEEEECCCCCHHHHHHHHCCHHHHCHHHHCC
DKTLEYVASKGQATASLNYPMGVSGTDAMLMKISELSGKAIPEAIEKERGRLVDAIADSQ
CHHHHHHHCCCCCEEEECCCCCCCCCHHHHHHHHHHCCCCCHHHHHHHHCCEEHHHHCCC
GWLHGKKAAIFGDPDFVLSMASFLMELGIEPTHCLCTNAGKEWDAQMKAVLAASPFGADC
CCCCCCCEEEECCHHHHHHHHHHHHHHCCCCCEEEECCCCCHHHHHHHHHHHCCCCCCCC
QVWAGKDLWHLRSLLFTEPTDMIIGSSYGKYLEKDTGIPLIRLTFPVFDRHHHHRFPTMG
EEECCCCHHHHHHHHHCCCCHHEECCHHHHHHHHCCCCCEEEEECHHHHCCCCCCCCCCC
YQGALRTLVEILDRIFEVADASSDISYDLTR
HHHHHHHHHHHHHHHHHHHCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9163424; 2744485 [H]