Click here to switch to the map view.

The map label for this gene is cysK [H]

Identifier: 83310399

GI number: 83310399

Start: 1419551

End: 1420273

Strand: Reverse

Name: cysK [H]

Synonym: amb1300

Alternate gene names: 83310399

Gene position: 1420273-1419551 (Counterclockwise)

Preceding gene: 83310400

Following gene: 83310398

Centisome position: 28.59

GC content: 63.49

Gene sequence:

>723_bases
ATGCCCGAGACCCAAAGCCAGGAAAAGAAGGACATGCTGCGGCTGGTGGGCGCCGACCTGCGTCTGGTTCCGGCCGTGCC
CTACGCCAATCCCGGCAATTACGTGCGCTATTCCGAGACCCTGGCCAACGAACTGGCCAAGACCGAGCCCAATGGCGTGC
TGTGGGCCAACCAGTTCGACAACACCGCCAACCGCATGGGCCACTTCAACACCACCGGCCAGGAGATCTGGCGCCAGACC
GACGGCACGGTGGATGCCTTCACCTGCGCGGTGGGGACCGGCGGCACCCTGGCGGGCACCGGCATGGCGCTGAAGGAGCA
CAACAAGGACATCAAGGTGGTGCTGGCCGACCCCATGGGCTCGGCGCTGTACCACCACTATGCCCATGGCGAGCTGAAGG
CCGAGGGCACCTCCATCACCGAAGGCATCGGCCAGGGCCGCATCACCCGCAATCTGGACGGCGCCCCCATCGACGATCAG
GTCCAGGTGACCGATCACGAGGCGCTGCCGCTGATTTTCGATCTGGTTCGGCAGGAGGGACTGGTTCTGGGAGGATCGAG
CGGAATCAATGTGATGGCCGCCATCAAGGTGGCGCGCATATTAGGACCCGGACACACAGTGGTGACAGTGCTCTGCGATT
ATGGTACCCGCTATCAAAGCAAGCTGTTCAATCCGGCCTTCCTGCGGGAACGCAATCTGCCGGTGCCCCATTGGCTGGAG
TGA

Upstream 100 bases:

>100_bases
GCTGAAGCCCGGCGGCGTGATCGTCGAGGGCACAGCGGGCAATACCGGCATCGGCCTGGCCCTGGTGGCCAATGCCCTGG
GCTACAGGACGGTCATCGTC

Downstream 100 bases:

>100_bases
GCGGCGACCAAACAAAAGCAAACCCGCAAGGGGGGAATTGAAGCATGATGCATCCCAATTCGGACGCCCTCGTCAGCACC
GAGTGGCTGGCCTCGCACCT

Product: cysteine synthase

Products: NA

Alternate protein names: CSase; O-acetylserine (thiol)-lyase; OAS-TL; O-acetylserine sulfhydrylase [H]

Number of amino acids: Translated: 240; Mature: 239

Protein sequence:

>240_residues
MPETQSQEKKDMLRLVGADLRLVPAVPYANPGNYVRYSETLANELAKTEPNGVLWANQFDNTANRMGHFNTTGQEIWRQT
DGTVDAFTCAVGTGGTLAGTGMALKEHNKDIKVVLADPMGSALYHHYAHGELKAEGTSITEGIGQGRITRNLDGAPIDDQ
VQVTDHEALPLIFDLVRQEGLVLGGSSGINVMAAIKVARILGPGHTVVTVLCDYGTRYQSKLFNPAFLRERNLPVPHWLE

Sequences:

>Translated_240_residues
MPETQSQEKKDMLRLVGADLRLVPAVPYANPGNYVRYSETLANELAKTEPNGVLWANQFDNTANRMGHFNTTGQEIWRQT
DGTVDAFTCAVGTGGTLAGTGMALKEHNKDIKVVLADPMGSALYHHYAHGELKAEGTSITEGIGQGRITRNLDGAPIDDQ
VQVTDHEALPLIFDLVRQEGLVLGGSSGINVMAAIKVARILGPGHTVVTVLCDYGTRYQSKLFNPAFLRERNLPVPHWLE
>Mature_239_residues
PETQSQEKKDMLRLVGADLRLVPAVPYANPGNYVRYSETLANELAKTEPNGVLWANQFDNTANRMGHFNTTGQEIWRQTD
GTVDAFTCAVGTGGTLAGTGMALKEHNKDIKVVLADPMGSALYHHYAHGELKAEGTSITEGIGQGRITRNLDGAPIDDQV
QVTDHEALPLIFDLVRQEGLVLGGSSGINVMAAIKVARILGPGHTVVTVLCDYGTRYQSKLFNPAFLRERNLPVPHWLE

Specific function: Cysteine biosynthesis. [C]

COG id: COG0031

COG function: function code E; Cysteine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the cysteine synthase/cystathionine beta- synthase family [H]

Homologues:

Organism=Homo sapiens, GI295821202, Length=240, Percent_Identity=34.1666666666667, Blast_Score=125, Evalue=4e-29,
Organism=Homo sapiens, GI295821200, Length=240, Percent_Identity=34.1666666666667, Blast_Score=125, Evalue=4e-29,
Organism=Homo sapiens, GI4557415, Length=240, Percent_Identity=34.1666666666667, Blast_Score=125, Evalue=4e-29,
Organism=Escherichia coli, GI1788754, Length=236, Percent_Identity=33.8983050847458, Blast_Score=96, Evalue=1e-21,
Organism=Escherichia coli, GI2367138, Length=221, Percent_Identity=30.316742081448, Blast_Score=89, Evalue=2e-19,
Organism=Caenorhabditis elegans, GI17535051, Length=240, Percent_Identity=33.75, Blast_Score=117, Evalue=4e-27,
Organism=Caenorhabditis elegans, GI32566672, Length=220, Percent_Identity=34.5454545454545, Blast_Score=111, Evalue=4e-25,
Organism=Caenorhabditis elegans, GI17562970, Length=220, Percent_Identity=34.5454545454545, Blast_Score=110, Evalue=5e-25,
Organism=Caenorhabditis elegans, GI32566674, Length=220, Percent_Identity=34.5454545454545, Blast_Score=110, Evalue=5e-25,
Organism=Caenorhabditis elegans, GI115535073, Length=221, Percent_Identity=36.1990950226244, Blast_Score=102, Evalue=2e-22,
Organism=Caenorhabditis elegans, GI17561720, Length=240, Percent_Identity=33.3333333333333, Blast_Score=86, Evalue=2e-17,
Organism=Caenorhabditis elegans, GI17534315, Length=237, Percent_Identity=29.1139240506329, Blast_Score=75, Evalue=4e-14,
Organism=Caenorhabditis elegans, GI25147552, Length=237, Percent_Identity=28.6919831223629, Blast_Score=66, Evalue=1e-11,
Organism=Caenorhabditis elegans, GI71996324, Length=241, Percent_Identity=27.8008298755187, Blast_Score=65, Evalue=4e-11,
Organism=Saccharomyces cerevisiae, GI6321449, Length=242, Percent_Identity=38.8429752066116, Blast_Score=134, Evalue=1e-32,
Organism=Saccharomyces cerevisiae, GI6321594, Length=246, Percent_Identity=30.4878048780488, Blast_Score=96, Evalue=3e-21,
Organism=Drosophila melanogaster, GI24643623, Length=244, Percent_Identity=30.7377049180328, Blast_Score=93, Evalue=1e-19,
Organism=Drosophila melanogaster, GI20129101, Length=244, Percent_Identity=30.7377049180328, Blast_Score=93, Evalue=1e-19,

Paralogues:

None

Copy number: 2820 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 240 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001216
- InterPro:   IPR005856
- InterPro:   IPR005859
- InterPro:   IPR001926 [H]

Pfam domain/function: PF00291 PALP [H]

EC number: =2.5.1.47 [H]

Molecular weight: Translated: 26135; Mature: 26004

Theoretical pI: Translated: 6.09; Mature: 6.09

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPETQSQEKKDMLRLVGADLRLVPAVPYANPGNYVRYSETLANELAKTEPNGVLWANQFD
CCCCCHHHHHHHHHHHCCCEEEEECCCCCCCCCEEEHHHHHHHHHHHCCCCCEEEECCCC
NTANRMGHFNTTGQEIWRQTDGTVDAFTCAVGTGGTLAGTGMALKEHNKDIKVVLADPMG
CHHHHCCCCCCCHHHHHHHCCCCEEEEEEECCCCCCCCCCCCCHHHCCCCEEEEEECCCC
SALYHHYAHGELKAEGTSITEGIGQGRITRNLDGAPIDDQVQVTDHEALPLIFDLVRQEG
HHHHHHHCCCEEEECCCHHHHHCCCCCEEECCCCCCCCCCEEECCCCCHHHHHHHHHHCC
LVLGGSSGINVMAAIKVARILGPGHTVVTVLCDYGTRYQSKLFNPAFLRERNLPVPHWLE
EEEECCCCCHHHHHHHHHHHHCCCCEEEEEEECCCCHHHHHCCCHHHHHCCCCCCCCCCC
>Mature Secondary Structure 
PETQSQEKKDMLRLVGADLRLVPAVPYANPGNYVRYSETLANELAKTEPNGVLWANQFD
CCCCHHHHHHHHHHHCCCEEEEECCCCCCCCCEEEHHHHHHHHHHHCCCCCEEEECCCC
NTANRMGHFNTTGQEIWRQTDGTVDAFTCAVGTGGTLAGTGMALKEHNKDIKVVLADPMG
CHHHHCCCCCCCHHHHHHHCCCCEEEEEEECCCCCCCCCCCCCHHHCCCCEEEEEECCCC
SALYHHYAHGELKAEGTSITEGIGQGRITRNLDGAPIDDQVQVTDHEALPLIFDLVRQEG
HHHHHHHCCCEEEECCCHHHHHCCCCCEEECCCCCCCCCCEEECCCCCHHHHHHHHHHCC
LVLGGSSGINVMAAIKVARILGPGHTVVTVLCDYGTRYQSKLFNPAFLRERNLPVPHWLE
EEEECCCCCHHHHHHHHHHHHCCCCEEEEEEECCCCHHHHHCCCHHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA