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The map label for this gene is sseA [H]

Identifier: 83310398

GI number: 83310398

Start: 1418652

End: 1419506

Strand: Reverse

Name: sseA [H]

Synonym: amb1299

Alternate gene names: 83310398

Gene position: 1419506-1418652 (Counterclockwise)

Preceding gene: 83310399

Following gene: 83310394

Centisome position: 28.58

GC content: 67.72

Gene sequence:

>855_bases
ATGATGCATCCCAATTCGGACGCCCTCGTCAGCACCGAGTGGCTGGCCTCGCACCTCAGCGCGCCCGACGTGCGCGTGGT
CGACGCCAGCTGGTACACGCCCGGCCAGAACCGCAACGCCCGCGAGGAATATGACGCCGAGCATATCCCCGGTGCCGTGT
TCTTCGACATCGACGAGATCGCCGACACCGATTCCACCCTGCCCCATATGCTGCCCGCGCCGGAGAAGTTCTCCAGCAAG
GTGCGCAAGCTGGGCCTGGGCAACGGCAACAAGATCGTGATCTACGACGGCTCGGGCTTTGCCAGCGCCGCGGCGCGGGC
CTGGTGGATGTTCCGCACCTTCGGCCACCGCGACGTGTCGGTGCTGGACGGCGGCTTCCCCAAGTGGCTGCGCGAAGGCC
TGCCGGTGGAAGACCTGCCGCCGGTGCCGCGCACCCGGCACTTCATCAGCCACTACAACCATCTGCTGGTCCGCGACCTG
GACCACATGAAGGCCAATCTGGAGAGCAAGCGCGAGTTGGTGATCGACGCCCGCGCCCCCGCCCGCTTCAAGGGCGAGGC
CGCCGAGCCTCGCCCCACCAAGCATCAGGGCCATATCCCCGGCTCGGTCAACGTGCCCTTCGCCGACCTGATCGACGAGC
GCACCCGCTGCATGCTGCCCACCGAGCAGTTGAAGGCCCGCTTCGACGCCGCCGGCATCGATTCCAAGCAGCCGGTGACC
ATCTCCTGCGGCTCGGGCGTCACCGCCTGCACCGTGGCCCTGGCGCTGCATCTGGTGGGGCACGAGAACGTGGCGGTCTA
TGACGGCTCCTGGGCCGAATGGGGCAACCGCGACGACACGCCCATCGAGAAGTAG

Upstream 100 bases:

>100_bases
TCAATCCGGCCTTCCTGCGGGAACGCAATCTGCCGGTGCCCCATTGGCTGGAGTGAGCGGCGACCAAACAAAAGCAAACC
CGCAAGGGGGGAATTGAAGC

Downstream 100 bases:

>100_bases
GTCCACGGCCAAGCCCGGACCAGGATGGATCAGCCCCCGCCCTCGCCGGCGGGGGTTTTTCGTTCAAGACGCCCGAATGG
CCGAGGATGGCGCGGCCTCG

Product: rhodanese-related sulfurtransferase

Products: NA

Alternate protein names: MST; Rhodanese-like protein [H]

Number of amino acids: Translated: 284; Mature: 284

Protein sequence:

>284_residues
MMHPNSDALVSTEWLASHLSAPDVRVVDASWYTPGQNRNAREEYDAEHIPGAVFFDIDEIADTDSTLPHMLPAPEKFSSK
VRKLGLGNGNKIVIYDGSGFASAAARAWWMFRTFGHRDVSVLDGGFPKWLREGLPVEDLPPVPRTRHFISHYNHLLVRDL
DHMKANLESKRELVIDARAPARFKGEAAEPRPTKHQGHIPGSVNVPFADLIDERTRCMLPTEQLKARFDAAGIDSKQPVT
ISCGSGVTACTVALALHLVGHENVAVYDGSWAEWGNRDDTPIEK

Sequences:

>Translated_284_residues
MMHPNSDALVSTEWLASHLSAPDVRVVDASWYTPGQNRNAREEYDAEHIPGAVFFDIDEIADTDSTLPHMLPAPEKFSSK
VRKLGLGNGNKIVIYDGSGFASAAARAWWMFRTFGHRDVSVLDGGFPKWLREGLPVEDLPPVPRTRHFISHYNHLLVRDL
DHMKANLESKRELVIDARAPARFKGEAAEPRPTKHQGHIPGSVNVPFADLIDERTRCMLPTEQLKARFDAAGIDSKQPVT
ISCGSGVTACTVALALHLVGHENVAVYDGSWAEWGNRDDTPIEK
>Mature_284_residues
MMHPNSDALVSTEWLASHLSAPDVRVVDASWYTPGQNRNAREEYDAEHIPGAVFFDIDEIADTDSTLPHMLPAPEKFSSK
VRKLGLGNGNKIVIYDGSGFASAAARAWWMFRTFGHRDVSVLDGGFPKWLREGLPVEDLPPVPRTRHFISHYNHLLVRDL
DHMKANLESKRELVIDARAPARFKGEAAEPRPTKHQGHIPGSVNVPFADLIDERTRCMLPTEQLKARFDAAGIDSKQPVT
ISCGSGVTACTVALALHLVGHENVAVYDGSWAEWGNRDDTPIEK

Specific function: Transfers a sulfur ion to cyanide or to other thiol compounds. Also has weak rhodanese activity. Its participation in detoxification of cyanide may be small. May be involved in the enhancement of serine sensitivity [H]

COG id: COG2897

COG function: function code P; Rhodanese-related sulfurtransferase

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 rhodanese domains [H]

Homologues:

Organism=Homo sapiens, GI194473668, Length=276, Percent_Identity=44.5652173913043, Blast_Score=241, Evalue=5e-64,
Organism=Homo sapiens, GI61835204, Length=276, Percent_Identity=44.5652173913043, Blast_Score=241, Evalue=6e-64,
Organism=Homo sapiens, GI194473681, Length=276, Percent_Identity=44.5652173913043, Blast_Score=241, Evalue=6e-64,
Organism=Homo sapiens, GI17402865, Length=276, Percent_Identity=47.1014492753623, Blast_Score=240, Evalue=1e-63,
Organism=Escherichia coli, GI87082121, Length=277, Percent_Identity=43.3212996389892, Blast_Score=227, Evalue=6e-61,
Organism=Escherichia coli, GI87081967, Length=298, Percent_Identity=24.8322147651007, Blast_Score=83, Evalue=2e-17,
Organism=Caenorhabditis elegans, GI17561888, Length=298, Percent_Identity=28.8590604026846, Blast_Score=119, Evalue=2e-27,
Organism=Caenorhabditis elegans, GI71997283, Length=302, Percent_Identity=25.4966887417219, Blast_Score=102, Evalue=3e-22,
Organism=Caenorhabditis elegans, GI17543836, Length=279, Percent_Identity=29.3906810035842, Blast_Score=96, Evalue=2e-20,
Organism=Caenorhabditis elegans, GI17543838, Length=279, Percent_Identity=29.3906810035842, Blast_Score=96, Evalue=2e-20,
Organism=Caenorhabditis elegans, GI115534702, Length=249, Percent_Identity=27.710843373494, Blast_Score=87, Evalue=1e-17,
Organism=Caenorhabditis elegans, GI17533113, Length=153, Percent_Identity=33.9869281045752, Blast_Score=82, Evalue=4e-16,
Organism=Caenorhabditis elegans, GI17559150, Length=251, Percent_Identity=25.8964143426295, Blast_Score=79, Evalue=3e-15,
Organism=Saccharomyces cerevisiae, GI6324825, Length=266, Percent_Identity=31.5789473684211, Blast_Score=132, Evalue=6e-32,

Paralogues:

None

Copy number: 20 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001763
- InterPro:   IPR001307 [H]

Pfam domain/function: PF00581 Rhodanese [H]

EC number: =2.8.1.2 [H]

Molecular weight: Translated: 31478; Mature: 31478

Theoretical pI: Translated: 6.32; Mature: 6.32

Prosite motif: PS00380 RHODANESE_1 ; PS00683 RHODANESE_2 ; PS50206 RHODANESE_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMHPNSDALVSTEWLASHLSAPDVRVVDASWYTPGQNRNAREEYDAEHIPGAVFFDIDEI
CCCCCCCCEEEHHHHHHHCCCCCEEEEECCCCCCCCCCCCHHHCCHHHCCCEEEEEHHHH
ADTDSTLPHMLPAPEKFSSKVRKLGLGNGNKIVIYDGSGFASAAARAWWMFRTFGHRDVS
CCCCCCCCCCCCCCHHHHHHHHHHCCCCCCEEEEECCCCHHHHHHHHHHHHHHCCCCCEE
VLDGGFPKWLREGLPVEDLPPVPRTRHFISHYNHLLVRDLDHMKANLESKRELVIDARAP
ECCCCCHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCC
ARFKGEAAEPRPTKHQGHIPGSVNVPFADLIDERTRCMLPTEQLKARFDAAGIDSKQPVT
CCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCCEE
ISCGSGVTACTVALALHLVGHENVAVYDGSWAEWGNRDDTPIEK
EECCCCHHHHHHHHHHHHHCCCCEEEECCCHHHCCCCCCCCCCC
>Mature Secondary Structure
MMHPNSDALVSTEWLASHLSAPDVRVVDASWYTPGQNRNAREEYDAEHIPGAVFFDIDEI
CCCCCCCCEEEHHHHHHHCCCCCEEEEECCCCCCCCCCCCHHHCCHHHCCCEEEEEHHHH
ADTDSTLPHMLPAPEKFSSKVRKLGLGNGNKIVIYDGSGFASAAARAWWMFRTFGHRDVS
CCCCCCCCCCCCCCHHHHHHHHHHCCCCCCEEEEECCCCHHHHHHHHHHHHHHCCCCCEE
VLDGGFPKWLREGLPVEDLPPVPRTRHFISHYNHLLVRDLDHMKANLESKRELVIDARAP
ECCCCCHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCC
ARFKGEAAEPRPTKHQGHIPGSVNVPFADLIDERTRCMLPTEQLKARFDAAGIDSKQPVT
CCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCCEE
ISCGSGVTACTVALALHLVGHENVAVYDGSWAEWGNRDDTPIEK
EECCCCHHHHHHHHHHHHHCCCCEEEECCCHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]