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The map label for this gene is hydA [H]

Identifier: 83310214

GI number: 83310214

Start: 1189416

End: 1190570

Strand: Reverse

Name: hydA [H]

Synonym: amb1115

Alternate gene names: 83310214

Gene position: 1190570-1189416 (Counterclockwise)

Preceding gene: 83310217

Following gene: 83310213

Centisome position: 23.97

GC content: 61.65

Gene sequence:

>1155_bases
ATGTCCCGGTTCATCTCGTTATCCTCGACCAACAAAATCCTGGCCATGATCGCCCCCGGGCAACGGCGCCCCATCAACTG
GAAGGGTACATATTCAAAGAAGCTATTTGATCGGCCTATTGCTTGCAACTACAATCACCCGCGCCAAATCGCCATAAACT
TCGAGCCATACAACAAATATATGATGAAAGAAGTGGCGGGTGGACGGAGGCCGGGGATGAATTTAAATTTATACTGGTTA
CAATGTGGCGGATGCGGTGGCGACACCATGTCTTTCCTGAATTCTGAGACTCCCGATATCTTGCAGTTATTCTCGGGACT
TGGGATCGATGTTGTTTATCACCCATCCCTCTCCAACACCTCGCAGGCCGAGCAGCACTCTCAACTTGAGGTTATATCTT
CCGGCGAGATGCCCCTCGACATCCTGGTGGTAGAGGGCGCAGTCCTTCGCGGTCCGGCCGGCACCGGGATGTACGACACC
AAGGGCCGCCGGCCGAAGAAGGATATCGTCGCGGCCCTGGCGCGTCAGGCGCGTTTCGTGGTCGCCGCCGGGACCTGCGC
CGCGTTCGGCGGATTCGGCGCCCGCACCGAGATCGAGGCCACCGGCCTGCAGTTTCACCGTTCCGAGCCCGGTGGGTTCC
TCGGCGCCGATTTCCGCGCGAAATCCGGCCTGCCGGTCCTCAACCTGTCGGGCTGTCCCTGCCACCCCCAGGTGCTGTCC
AGCGTGCTGACCGCCCTGGCCACCGGCGCCGAGTTGCCCCTCGCCCCCCTGAACCGCCCGCTGCCATGGTACGGCATGAT
GGTTCACCAGGGCTGCACCAGAAACGAGTATCACGAGTACAGGGTCGAGGAAGACAATTTCGGCGAGAAGGGCTGCCTGT
TCTTCCACATGGGCTGCCACGGCCCCCTGGTCACCGGCCCCTGCAACAAGCTTCTGTGGAACCAGCGCTCATCCAAGACC
CGGGCCGGCGTCCCCTGCTTCGGCTGTACCAGCCCGGAATTCCCGCAGGACCACGCCTTCTTCGAGACCCGCAACATCGA
GGGCATCCCCCTTGACCTCCCCAACGGGATCAACCGCGCCCATTACATGGTCTACAAGGTCATGGCCGCCGCCGCCGCGC
CCGAGCGCCTGCGCCAGCGCCGCACCGACATTTAG

Upstream 100 bases:

>100_bases
GATCAGGTCGGGATTCTCGCTGGCCGCCATGGCGACGCCTTCGCCGCCATCCACCGCCATCACCACCTCGTAGCCCTTGC
GCTGCAACCGGCGGGACAAC

Downstream 100 bases:

>100_bases
GGGGGGGGAAAACCGATGGCCGTTCGCAAGATACTCGATGTTCCCGTCAACCGGGTCGAGGGCGACCTGGAACTGCGCCT
GGAGATCGCCGACGGCATGG

Product: Ni,Fe-hydrogenase I small subunit

Products: NA

Alternate protein names: NiFe hydrogenlyase small chain [H]

Number of amino acids: Translated: 384; Mature: 383

Protein sequence:

>384_residues
MSRFISLSSTNKILAMIAPGQRRPINWKGTYSKKLFDRPIACNYNHPRQIAINFEPYNKYMMKEVAGGRRPGMNLNLYWL
QCGGCGGDTMSFLNSETPDILQLFSGLGIDVVYHPSLSNTSQAEQHSQLEVISSGEMPLDILVVEGAVLRGPAGTGMYDT
KGRRPKKDIVAALARQARFVVAAGTCAAFGGFGARTEIEATGLQFHRSEPGGFLGADFRAKSGLPVLNLSGCPCHPQVLS
SVLTALATGAELPLAPLNRPLPWYGMMVHQGCTRNEYHEYRVEEDNFGEKGCLFFHMGCHGPLVTGPCNKLLWNQRSSKT
RAGVPCFGCTSPEFPQDHAFFETRNIEGIPLDLPNGINRAHYMVYKVMAAAAAPERLRQRRTDI

Sequences:

>Translated_384_residues
MSRFISLSSTNKILAMIAPGQRRPINWKGTYSKKLFDRPIACNYNHPRQIAINFEPYNKYMMKEVAGGRRPGMNLNLYWL
QCGGCGGDTMSFLNSETPDILQLFSGLGIDVVYHPSLSNTSQAEQHSQLEVISSGEMPLDILVVEGAVLRGPAGTGMYDT
KGRRPKKDIVAALARQARFVVAAGTCAAFGGFGARTEIEATGLQFHRSEPGGFLGADFRAKSGLPVLNLSGCPCHPQVLS
SVLTALATGAELPLAPLNRPLPWYGMMVHQGCTRNEYHEYRVEEDNFGEKGCLFFHMGCHGPLVTGPCNKLLWNQRSSKT
RAGVPCFGCTSPEFPQDHAFFETRNIEGIPLDLPNGINRAHYMVYKVMAAAAAPERLRQRRTDI
>Mature_383_residues
SRFISLSSTNKILAMIAPGQRRPINWKGTYSKKLFDRPIACNYNHPRQIAINFEPYNKYMMKEVAGGRRPGMNLNLYWLQ
CGGCGGDTMSFLNSETPDILQLFSGLGIDVVYHPSLSNTSQAEQHSQLEVISSGEMPLDILVVEGAVLRGPAGTGMYDTK
GRRPKKDIVAALARQARFVVAAGTCAAFGGFGARTEIEATGLQFHRSEPGGFLGADFRAKSGLPVLNLSGCPCHPQVLSS
VLTALATGAELPLAPLNRPLPWYGMMVHQGCTRNEYHEYRVEEDNFGEKGCLFFHMGCHGPLVTGPCNKLLWNQRSSKTR
AGVPCFGCTSPEFPQDHAFFETRNIEGIPLDLPNGINRAHYMVYKVMAAAAAPERLRQRRTDI

Specific function: This Is One Of Three E.Coli Hydrogenases Synthesized In Response To Different Physiological Conditions. Hyd1 Is Believed To Have A Role In Hydrogen Cycling During Fermentative Growth. [C]

COG id: COG1740

COG function: function code C; Ni,Fe-hydrogenase I small subunit

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the [NiFe]/[NiFeSe] hydrogenase small subunit family [H]

Homologues:

Organism=Escherichia coli, GI1787206, Length=293, Percent_Identity=25.938566552901, Blast_Score=79, Evalue=4e-16,
Organism=Escherichia coli, GI1789371, Length=299, Percent_Identity=26.0869565217391, Blast_Score=71, Evalue=1e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006137
- InterPro:   IPR001821 [H]

Pfam domain/function: PF01058 Oxidored_q6 [H]

EC number: =1.12.2.1 [H]

Molecular weight: Translated: 42228; Mature: 42097

Theoretical pI: Translated: 8.65; Mature: 8.65

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.1 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
6.5 %Cys+Met (Translated Protein)
3.1 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
6.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSRFISLSSTNKILAMIAPGQRRPINWKGTYSKKLFDRPIACNYNHPRQIAINFEPYNKY
CCCEEEECCCCEEEEEECCCCCCCCCCCCCHHHHHHCCCEEECCCCCEEEEEEECHHHHH
MMKEVAGGRRPGMNLNLYWLQCGGCGGDTMSFLNSETPDILQLFSGLGIDVVYHPSLSNT
HHHHHCCCCCCCCEEEEEEEEECCCCCHHHHHHCCCCHHHHHHHHCCCEEEEECCCCCCC
SQAEQHSQLEVISSGEMPLDILVVEGAVLRGPAGTGMYDTKGRRPKKDIVAALARQARFV
HHHHHHCCEEEEECCCCCEEEEEECCEEEECCCCCCCCCCCCCCCHHHHHHHHHHHCEEE
VAAGTCAAFGGFGARTEIEATGLQFHRSEPGGFLGADFRAKSGLPVLNLSGCPCHPQVLS
EEECCHHHHCCCCCCCEEEECCEEEECCCCCCEECCCCCCCCCCCEEECCCCCCCHHHHH
SVLTALATGAELPLAPLNRPLPWYGMMVHQGCTRNEYHEYRVEEDNFGEKGCLFFHMGCH
HHHHHHHCCCCCCCCCCCCCCCHHHHHHHCCCCCCCCHHEECCCCCCCCCCEEEEEECCC
GPLVTGPCNKLLWNQRSSKTRAGVPCFGCTSPEFPQDHAFFETRNIEGIPLDLPNGINRA
CCEEECCHHHHHHCCCCCCCCCCCCEECCCCCCCCCCCCEEEECCCCCCCCCCCCCCCHH
HYMVYKVMAAAAAPERLRQRRTDI
HHHHHHHHHHHCCHHHHHHHHCCC
>Mature Secondary Structure 
SRFISLSSTNKILAMIAPGQRRPINWKGTYSKKLFDRPIACNYNHPRQIAINFEPYNKY
CCEEEECCCCEEEEEECCCCCCCCCCCCCHHHHHHCCCEEECCCCCEEEEEEECHHHHH
MMKEVAGGRRPGMNLNLYWLQCGGCGGDTMSFLNSETPDILQLFSGLGIDVVYHPSLSNT
HHHHHCCCCCCCCEEEEEEEEECCCCCHHHHHHCCCCHHHHHHHHCCCEEEEECCCCCCC
SQAEQHSQLEVISSGEMPLDILVVEGAVLRGPAGTGMYDTKGRRPKKDIVAALARQARFV
HHHHHHCCEEEEECCCCCEEEEEECCEEEECCCCCCCCCCCCCCCHHHHHHHHHHHCEEE
VAAGTCAAFGGFGARTEIEATGLQFHRSEPGGFLGADFRAKSGLPVLNLSGCPCHPQVLS
EEECCHHHHCCCCCCCEEEECCEEEECCCCCCEECCCCCCCCCCCEEECCCCCCCHHHHH
SVLTALATGAELPLAPLNRPLPWYGMMVHQGCTRNEYHEYRVEEDNFGEKGCLFFHMGCH
HHHHHHHCCCCCCCCCCCCCCCHHHHHHHCCCCCCCCHHEECCCCCCCCCCEEEEEECCC
GPLVTGPCNKLLWNQRSSKTRAGVPCFGCTSPEFPQDHAFFETRNIEGIPLDLPNGINRA
CCEEECCHHHHHHCCCCCCCCCCCCEECCCCCCCCCCCCEEEECCCCCCCCCCCCCCCHH
HYMVYKVMAAAAAPERLRQRRTDI
HHHHHHHHHHHCCHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 3322743; 2651421; 3322275; 3134950; 7854413 [H]