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The map label for this gene is dacF [H]

Identifier: 83310217

GI number: 83310217

Start: 1192966

End: 1194273

Strand: Reverse

Name: dacF [H]

Synonym: amb1118

Alternate gene names: 83310217

Gene position: 1194273-1192966 (Counterclockwise)

Preceding gene: 83310219

Following gene: 83310214

Centisome position: 24.04

GC content: 67.51

Gene sequence:

>1308_bases
TTGCACCTGAGCCGCACCGGATTCCTGAACCGCCTTCGCCTGATTGCCGTCGCCCTGGGCGTCGCGATCATCGCCGTGGC
CCAGCCGGCCGAGGCCGGACGCTACGCCTCCATCGTCATCGATGCGGAAAACGGTTCCGTCCTGCATGCGGCCAATCCCG
ACACGAGATCCTATCCGGCATCCCTGACCAAGGTGATGACCCTGTTCCTGCTGTTCGACGAGCTGGATTCCGGGCGCATC
CGGCTGGATTCCAAGTTCACCACCTCGGCCCATGCCGCCGCCCAGTCGCCGTCCAAGCTGGGGCTCGAACCCGGCGAAAA
GATTTCCGTGGAAAGCCTGATCCTCGCCCTGGTCACCAAGTCGGCCAACGATGCCGCCGTGGTGGCGGCCGAAGGCGTGG
GCGGGACCGAGACCCGCTTCGCCCATATGATGACCCAGAAGGCTCATGCGCTGGGCATGCGCAACACGGTCTACCGCAAC
GCCTCGGGGCTGCCCGATACCGGCCAGGTGTCCACGGTGCGCGACCAGGCCACCCTGGCCCGCGCCCTGATCCGCAGCCA
TCCCGGCTATTACAAATATTTTTCCACCCGTCAGTTCGTCTATGACGGCCAGCCCATCAACACCCATAACCGACTGATGT
TGCGCTATCAGGGCGCCGACGGCATCAAGACCGGCTATATCCACGCCTCGGGCTTCAATCTCATCTCGTCGGCCAAGCGC
GGCGACAAGCGCATCATCGGCGTGGTGTTCGGCGGCAGCACCGCCTCGTCGCGCGACAACCACATGGGCCAGCTGCTGGA
CAAGGGCTTCGCCAAGCTGCGGCGGGGCGGCGGCGTCGAGATGGCGGCCGCCGAGACCGAGACCGAGGGCAACGACGACC
TGCCCGACCTGGACGACCTGGAGGCCGCCGCCCAGGCGGCCAAGGCCCCGGCCAAGGCTCCGGCCAAGGCCGTCGCCAAG
GCCAAGCCGGCTCCCGCCAAGAAGGTCGTCCTGCGTGCCCCCGCCCGCGCCGCCGATGACGACGACGACGACGACGACGC
GGTGGGCGATGCCGACCCGTCGGGCTGGGCCATCCAGGTGGGGGCGTTCAGCGAGTACCGTCCGGCCCACAAGGCGGCCA
GCGACGCGGCCAAGAAGCTGGGTGGGCTGGTGTCCAAGGCCTCCATCGACATCGACAAGGCCGGCAAGGGCGCCAAGGCC
CTCTATCGCGCCCGCATTTCCGGCTTTACCGAGGATCAGGCCCGTGCCGCCTGCAAGCGCCTGGGCAAGGCCGGAAAGTC
CTGCAAGACGGTCAATCCCAACGCCTGA

Upstream 100 bases:

>100_bases
GGGAATCGGGCGTCCGCGACGCTTTACACCGATTCGCAAAATCTTTAAGGTTTGCGCGATACCCTAAACCAACTCAGTCG
TTGAACGGAAGGTCGAGACG

Downstream 100 bases:

>100_bases
GGCGATCGGCCAGCCCCGGCGGCGTCACAGCGCCGCCAGCCCCACGTCGACCGATGGATGAACCTTGAAGATGCGCAGCA
GGCCGCTGACCTCGAACACT

Product: D-alanyl-D-alanine carboxypeptidase

Products: NA

Alternate protein names: DD-carboxypeptidase; DD-peptidase; Penicillin-binding protein dacF; PBP [H]

Number of amino acids: Translated: 435; Mature: 435

Protein sequence:

>435_residues
MHLSRTGFLNRLRLIAVALGVAIIAVAQPAEAGRYASIVIDAENGSVLHAANPDTRSYPASLTKVMTLFLLFDELDSGRI
RLDSKFTTSAHAAAQSPSKLGLEPGEKISVESLILALVTKSANDAAVVAAEGVGGTETRFAHMMTQKAHALGMRNTVYRN
ASGLPDTGQVSTVRDQATLARALIRSHPGYYKYFSTRQFVYDGQPINTHNRLMLRYQGADGIKTGYIHASGFNLISSAKR
GDKRIIGVVFGGSTASSRDNHMGQLLDKGFAKLRRGGGVEMAAAETETEGNDDLPDLDDLEAAAQAAKAPAKAPAKAVAK
AKPAPAKKVVLRAPARAADDDDDDDDAVGDADPSGWAIQVGAFSEYRPAHKAASDAAKKLGGLVSKASIDIDKAGKGAKA
LYRARISGFTEDQARAACKRLGKAGKSCKTVNPNA

Sequences:

>Translated_435_residues
MHLSRTGFLNRLRLIAVALGVAIIAVAQPAEAGRYASIVIDAENGSVLHAANPDTRSYPASLTKVMTLFLLFDELDSGRI
RLDSKFTTSAHAAAQSPSKLGLEPGEKISVESLILALVTKSANDAAVVAAEGVGGTETRFAHMMTQKAHALGMRNTVYRN
ASGLPDTGQVSTVRDQATLARALIRSHPGYYKYFSTRQFVYDGQPINTHNRLMLRYQGADGIKTGYIHASGFNLISSAKR
GDKRIIGVVFGGSTASSRDNHMGQLLDKGFAKLRRGGGVEMAAAETETEGNDDLPDLDDLEAAAQAAKAPAKAPAKAVAK
AKPAPAKKVVLRAPARAADDDDDDDDAVGDADPSGWAIQVGAFSEYRPAHKAASDAAKKLGGLVSKASIDIDKAGKGAKA
LYRARISGFTEDQARAACKRLGKAGKSCKTVNPNA
>Mature_435_residues
MHLSRTGFLNRLRLIAVALGVAIIAVAQPAEAGRYASIVIDAENGSVLHAANPDTRSYPASLTKVMTLFLLFDELDSGRI
RLDSKFTTSAHAAAQSPSKLGLEPGEKISVESLILALVTKSANDAAVVAAEGVGGTETRFAHMMTQKAHALGMRNTVYRN
ASGLPDTGQVSTVRDQATLARALIRSHPGYYKYFSTRQFVYDGQPINTHNRLMLRYQGADGIKTGYIHASGFNLISSAKR
GDKRIIGVVFGGSTASSRDNHMGQLLDKGFAKLRRGGGVEMAAAETETEGNDDLPDLDDLEAAAQAAKAPAKAPAKAVAK
AKPAPAKKVVLRAPARAADDDDDDDDAVGDADPSGWAIQVGAFSEYRPAHKAASDAAKKLGGLVSKASIDIDKAGKGAKA
LYRARISGFTEDQARAACKRLGKAGKSCKTVNPNA

Specific function: Removes C-terminal D-alanyl residues from sugar-peptide cell wall precursors [H]

COG id: COG1686

COG function: function code M; D-alanyl-D-alanine carboxypeptidase

Gene ontology:

Cell location: Secreted (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase S11 family [H]

Homologues:

Organism=Escherichia coli, GI1787062, Length=242, Percent_Identity=35.1239669421488, Blast_Score=141, Evalue=9e-35,
Organism=Escherichia coli, GI1786851, Length=240, Percent_Identity=34.5833333333333, Blast_Score=141, Evalue=1e-34,
Organism=Escherichia coli, GI87082022, Length=249, Percent_Identity=34.136546184739, Blast_Score=139, Evalue=5e-34,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012338
- InterPro:   IPR015956
- InterPro:   IPR018044
- InterPro:   IPR012907
- InterPro:   IPR001967 [H]

Pfam domain/function: PF07943 PBP5_C; PF00768 Peptidase_S11 [H]

EC number: =3.4.16.4 [H]

Molecular weight: Translated: 45813; Mature: 45813

Theoretical pI: Translated: 9.93; Mature: 9.93

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHLSRTGFLNRLRLIAVALGVAIIAVAQPAEAGRYASIVIDAENGSVLHAANPDTRSYPA
CCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEEECCCCCEEEECCCCCCCCHH
SLTKVMTLFLLFDELDSGRIRLDSKFTTSAHAAAQSPSKLGLEPGEKISVESLILALVTK
HHHHHHHHHHHHHCCCCCEEEECCCCCCHHHHHHCCCHHCCCCCCCCCCHHHHHHHHHHC
SANDAAVVAAEGVGGTETRFAHMMTQKAHALGMRNTVYRNASGLPDTGQVSTVRDQATLA
CCCCCEEEEECCCCCCHHHHHHHHHHHHHHHCCHHHHHCCCCCCCCCCCCCHHHHHHHHH
RALIRSHPGYYKYFSTRQFVYDGQPINTHNRLMLRYQGADGIKTGYIHASGFNLISSAKR
HHHHHCCCCCEEEECCEEEEECCCCCCCCCEEEEEECCCCCCEEEEEEECCCHHHHHHCC
GDKRIIGVVFGGSTASSRDNHMGQLLDKGFAKLRRGGGVEMAAAETETEGNDDLPDLDDL
CCCEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCCCCCCCCHHHH
EAAAQAAKAPAKAPAKAVAKAKPAPAKKVVLRAPARAADDDDDDDDAVGDADPSGWAIQV
HHHHHHHCCCCCCCHHHHHHCCCCCCHHEEEECCCCCCCCCCCCCCCCCCCCCCCCEEEE
GAFSEYRPAHKAASDAAKKLGGLVSKASIDIDKAGKGAKALYRARISGFTEDQARAACKR
CCCHHCCHHHHHHHHHHHHHHHHHHHHCCCHHCCCCCHHHHHHHHHCCCCHHHHHHHHHH
LGKAGKSCKTVNPNA
HHHCCCCCCCCCCCC
>Mature Secondary Structure
MHLSRTGFLNRLRLIAVALGVAIIAVAQPAEAGRYASIVIDAENGSVLHAANPDTRSYPA
CCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEEECCCCCEEEECCCCCCCCHH
SLTKVMTLFLLFDELDSGRIRLDSKFTTSAHAAAQSPSKLGLEPGEKISVESLILALVTK
HHHHHHHHHHHHHCCCCCEEEECCCCCCHHHHHHCCCHHCCCCCCCCCCHHHHHHHHHHC
SANDAAVVAAEGVGGTETRFAHMMTQKAHALGMRNTVYRNASGLPDTGQVSTVRDQATLA
CCCCCEEEEECCCCCCHHHHHHHHHHHHHHHCCHHHHHCCCCCCCCCCCCCHHHHHHHHH
RALIRSHPGYYKYFSTRQFVYDGQPINTHNRLMLRYQGADGIKTGYIHASGFNLISSAKR
HHHHHCCCCCEEEECCEEEEECCCCCCCCCEEEEEECCCCCCEEEEEEECCCHHHHHHCC
GDKRIIGVVFGGSTASSRDNHMGQLLDKGFAKLRRGGGVEMAAAETETEGNDDLPDLDDL
CCCEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCCCCCCCCHHHH
EAAAQAAKAPAKAPAKAVAKAKPAPAKKVVLRAPARAADDDDDDDDAVGDADPSGWAIQV
HHHHHHHCCCCCCCHHHHHHCCCCCCHHEEEECCCCCCCCCCCCCCCCCCCCCCCCEEEE
GAFSEYRPAHKAASDAAKKLGGLVSKASIDIDKAGKGAKALYRARISGFTEDQARAACKR
CCCHHCCHHHHHHHHHHHHHHHHHHHHCCCHHCCCCCHHHHHHHHHCCCCHHHHHHHHHH
LGKAGKSCKTVNPNA
HHHCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1629150; 8969508; 9384377 [H]