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The map label for this gene is hydB [H]

Identifier: 83310213

GI number: 83310213

Start: 1187862

End: 1189400

Strand: Reverse

Name: hydB [H]

Synonym: amb1114

Alternate gene names: 83310213

Gene position: 1189400-1187862 (Counterclockwise)

Preceding gene: 83310214

Following gene: 83310212

Centisome position: 23.95

GC content: 71.09

Gene sequence:

>1539_bases
ATGGCCGTTCGCAAGATACTCGATGTTCCCGTCAACCGGGTCGAGGGCGACCTGGAACTGCGCCTGGAGATCGCCGACGG
CATGGTGGTCGACGCCTGGAGCGCCGGCACCATGTTCCGCGGCTTCGAACGCCTGCTGATCGGGCGGGGCGCCCTGGACG
GGCTGGTGGTGACGCCCCGCATCTGCGGCATCTGCAGCACCACCCACCTGATGACGGCGGCCAAGGCGCTCGACGCGGTG
GCCGGGGCCAAGGTGCCCGACAACGGCGTCCGGCTGCGCAACCTCGCCCTGATGGCCGAGCATGTCCAAAGCGACGTTCG
CCAGGGCATCCTGATGTTCCTGGTGGATTTCGCCAATCCCGCCTACCGCGCCCAGCCGCTGTATGACGAGGCGGTGCGCC
GCTATGCCCCGCTGGCCGGCGAGGCCGTCGCCGAGGCGGTGCGCGAGACCAAGCATGTGGTCGAGATCATCGCCATCATC
GGCGGCCAGTGGCCCCACAGCTCGTTCATGGTACCGGGCGGCCTGTCCTATGCCCCGCCGGTGGCCGAGTTGCTGCAGTG
CCGCCATGTCCTCGATGGCTTCCGGGGGTGGTACGAGCGGCGCATCCTCGGTTGTTCCCTGGACCGCTGGCGAGCGGTGG
ACAGCCTGGAGGCGCTGGACCTCTGGCTGGAGGAATCCGCCGCCCATCGCGACAGCGACGTGGGCTTCCTGCTGCGCTTC
GGCCGGACCATCGGGCTGGACCGCCTGGGAGGGGGGCACGGCAATTTCCTGTCCTGCGGCTCGCTGGACCTGCCGGCGGA
GACGGCGGCGCGGGGCGTCGGCGGCCATCTGGTCCCGTCGGGCTTCGCCCGGGGCGCGGTGGTGGAGGGCTTCGACCCAG
CGGCCATTGCCGAGCATGTGGCGGCGTCCTGGTACGCGGCCTACGAGGGCGGGCGCCACCCGGCCGAGGGCGAGACCGAG
CCCTATGCCTCGGGCGAGGAGGGACGCCGCTATTCCTGGGTCAAGGCCGCCCGCTATCTCGGCACCCCGGCGGAGACCGG
CCCCTTCGCCGAGCGGATCGTGGCGGGCGACCCGCTGTTCACGGACCTGCTGCGCCGGCAGGGGCCCAATGCCCTGGCCC
GCGAGCTGGCCCGCCTGACCCGGCCCGCCACGCTGCTGCCCGCCATGGCGTTGTGGCTTGGCGAGGTCATCGCCAGCGGC
GGCCACGACATCTATGCCGATCCCGGCCCCATCCCCGACGGCGCCGGCATCGGCATGATTCAGGCGGCGCGGGGCGCGCT
GGGTCACTGGATAGCCATCAAGGACGGACGGATCGAACGCTACCAGATCATCACGCCCACCGTGTGGAACGGCTCGCCCC
GGGATTCGGGCGGGGTGCGCGGTCCGTGGGAGGAAGCCCTGATCGGCACGCCGGTGGCGGACGCAGACAATCCCGTGGAG
GTCGGCCATGTGATCCGCTCCTTCGACCCCTGTCTGGTCTGTTCCGTCCATACGGTACGCAAGGGCCGAAGCCTCGGCCG
CGTGCGTCTGGAGGCCTGA

Upstream 100 bases:

>100_bases
CAACCGCGCCCATTACATGGTCTACAAGGTCATGGCCGCCGCCGCCGCGCCCGAGCGCCTGCGCCAGCGCCGCACCGACA
TTTAGGGGGGGGGAAAACCG

Downstream 100 bases:

>100_bases
GATGGGCCGCCCGCGCCGCATCATCTGCGTCGGCAACCGCCATCGTCCCGACGACGATCTCGGCCCCCGCGTCCACGACC
TGCTGGCCGGGGCGGCACTG

Product: Ni,Fe-hydrogenase I large subunit

Products: NA

Alternate protein names: NiFe hydrogenlyase large chain [H]

Number of amino acids: Translated: 512; Mature: 511

Protein sequence:

>512_residues
MAVRKILDVPVNRVEGDLELRLEIADGMVVDAWSAGTMFRGFERLLIGRGALDGLVVTPRICGICSTTHLMTAAKALDAV
AGAKVPDNGVRLRNLALMAEHVQSDVRQGILMFLVDFANPAYRAQPLYDEAVRRYAPLAGEAVAEAVRETKHVVEIIAII
GGQWPHSSFMVPGGLSYAPPVAELLQCRHVLDGFRGWYERRILGCSLDRWRAVDSLEALDLWLEESAAHRDSDVGFLLRF
GRTIGLDRLGGGHGNFLSCGSLDLPAETAARGVGGHLVPSGFARGAVVEGFDPAAIAEHVAASWYAAYEGGRHPAEGETE
PYASGEEGRRYSWVKAARYLGTPAETGPFAERIVAGDPLFTDLLRRQGPNALARELARLTRPATLLPAMALWLGEVIASG
GHDIYADPGPIPDGAGIGMIQAARGALGHWIAIKDGRIERYQIITPTVWNGSPRDSGGVRGPWEEALIGTPVADADNPVE
VGHVIRSFDPCLVCSVHTVRKGRSLGRVRLEA

Sequences:

>Translated_512_residues
MAVRKILDVPVNRVEGDLELRLEIADGMVVDAWSAGTMFRGFERLLIGRGALDGLVVTPRICGICSTTHLMTAAKALDAV
AGAKVPDNGVRLRNLALMAEHVQSDVRQGILMFLVDFANPAYRAQPLYDEAVRRYAPLAGEAVAEAVRETKHVVEIIAII
GGQWPHSSFMVPGGLSYAPPVAELLQCRHVLDGFRGWYERRILGCSLDRWRAVDSLEALDLWLEESAAHRDSDVGFLLRF
GRTIGLDRLGGGHGNFLSCGSLDLPAETAARGVGGHLVPSGFARGAVVEGFDPAAIAEHVAASWYAAYEGGRHPAEGETE
PYASGEEGRRYSWVKAARYLGTPAETGPFAERIVAGDPLFTDLLRRQGPNALARELARLTRPATLLPAMALWLGEVIASG
GHDIYADPGPIPDGAGIGMIQAARGALGHWIAIKDGRIERYQIITPTVWNGSPRDSGGVRGPWEEALIGTPVADADNPVE
VGHVIRSFDPCLVCSVHTVRKGRSLGRVRLEA
>Mature_511_residues
AVRKILDVPVNRVEGDLELRLEIADGMVVDAWSAGTMFRGFERLLIGRGALDGLVVTPRICGICSTTHLMTAAKALDAVA
GAKVPDNGVRLRNLALMAEHVQSDVRQGILMFLVDFANPAYRAQPLYDEAVRRYAPLAGEAVAEAVRETKHVVEIIAIIG
GQWPHSSFMVPGGLSYAPPVAELLQCRHVLDGFRGWYERRILGCSLDRWRAVDSLEALDLWLEESAAHRDSDVGFLLRFG
RTIGLDRLGGGHGNFLSCGSLDLPAETAARGVGGHLVPSGFARGAVVEGFDPAAIAEHVAASWYAAYEGGRHPAEGETEP
YASGEEGRRYSWVKAARYLGTPAETGPFAERIVAGDPLFTDLLRRQGPNALARELARLTRPATLLPAMALWLGEVIASGG
HDIYADPGPIPDGAGIGMIQAARGALGHWIAIKDGRIERYQIITPTVWNGSPRDSGGVRGPWEEALIGTPVADADNPVEV
GHVIRSFDPCLVCSVHTVRKGRSLGRVRLEA

Specific function: Catalyzes the reversible oxidoreduction of molecular hydrogen, in conjunction with a specific electron acceptor, cytochrome c3 [H]

COG id: COG0374

COG function: function code C; Ni,Fe-hydrogenase I large subunit

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the [NiFe]/[NiFeSe] hydrogenase large subunit family [H]

Homologues:

Organism=Escherichia coli, GI1789368, Length=582, Percent_Identity=27.6632302405498, Blast_Score=196, Evalue=4e-51,
Organism=Escherichia coli, GI1787207, Length=577, Percent_Identity=27.0363951473137, Blast_Score=181, Evalue=1e-46,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001501
- InterPro:   IPR018194 [H]

Pfam domain/function: PF00374 NiFeSe_Hases [H]

EC number: =1.12.2.1 [H]

Molecular weight: Translated: 55096; Mature: 54964

Theoretical pI: Translated: 6.28; Mature: 6.28

Prosite motif: PS00507 NI_HGENASE_L_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAVRKILDVPVNRVEGDLELRLEIADGMVVDAWSAGTMFRGFERLLIGRGALDGLVVTPR
CCHHHHHHCCHHHCCCCEEEEEEECCCEEEECCCCCHHHHHHHHHHHCCCCCCCEEECCH
ICGICSTTHLMTAAKALDAVAGAKVPDNGVRLRNLALMAEHVQSDVRQGILMFLVDFANP
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
AYRAQPLYDEAVRRYAPLAGEAVAEAVRETKHVVEIIAIIGGQWPHSSFMVPGGLSYAPP
CCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEECCCCCCCCCH
VAELLQCRHVLDGFRGWYERRILGCSLDRWRAVDSLEALDLWLEESAAHRDSDVGFLLRF
HHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHH
GRTIGLDRLGGGHGNFLSCGSLDLPAETAARGVGGHLVPSGFARGAVVEGFDPAAIAEHV
CCHHCCCCCCCCCCCEEECCCCCCCHHHHHCCCCCCCCCCCCCCCCEECCCCHHHHHHHH
AASWYAAYEGGRHPAEGETEPYASGEEGRRYSWVKAARYLGTPAETGPFAERIVAGDPLF
HHHHHHHHCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCCCHHHHHHHCCCHHH
TDLLRRQGPNALARELARLTRPATLLPAMALWLGEVIASGGHDIYADPGPIPDGAGIGMI
HHHHHHCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCEECCCCCCCCCCCCCCHH
QAARGALGHWIAIKDGRIERYQIITPTVWNGSPRDSGGVRGPWEEALIGTPVADADNPVE
HHHCCCCEEEEEEECCCCEEEEEEECEEECCCCCCCCCCCCCHHHHHCCCCCCCCCCHHH
VGHVIRSFDPCLVCSVHTVRKGRSLGRVRLEA
HHHHHHHCCCCEEEEHHHHHCCCCCCCEEECC
>Mature Secondary Structure 
AVRKILDVPVNRVEGDLELRLEIADGMVVDAWSAGTMFRGFERLLIGRGALDGLVVTPR
CHHHHHHCCHHHCCCCEEEEEEECCCEEEECCCCCHHHHHHHHHHHCCCCCCCEEECCH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HHHHHHHCCCCEEEEHHHHHCCCCCCCEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2269874; 9438867 [H]